• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.32-43
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• 2003

UV irradiation on a skin brings about the qualitative and quantitative alterations of the extracellular matrix. Repeated-UV irradiation suppressed the synthesis of collagen and activated the expression of the matrix metalloprotease (MMP). In this paper, on the purpose of development of novel anti-aging agents from natural sources, effects of several natural products on in vitro MMP-1 activity and UVA induced MMP-1 synthesis in human dermal fibroblast (HDF) culture were studied. We measured MMP-1 activities by fluorescence assay using gelatin as substrates. As a result, the extract of Dicentra spectabilis, and flower buds of Tussilago farfara showed strong inhibitory effect. Among them, the extract of flower buds of Tussilago fartara and Dicentra spectabilis inhibited MMP-1 activity by 92% and 87% at 0.05% (w/v). And UVA induced MMP-1 expression were analyzed by enzyme-linked immunosorbent assay (ELISA) and gelatin-based zymography in HDF culture. The extract of flower buds of Tussilago farfara and Dicentra spectabilis suppressed the UVA induced expression of MMP-1 by similar level of Vitamin C 200$\mu$M at 0.1% (w/v). These results suggest that the extract of Dicentra spectabilis, and flower buds of Tussilago farfara effectively prevent skin from the UV-induced photoaging. So the extracts are thought to have potential as effective raw materials for anti-aging cosmetics.

• 농업과학연구
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• 제41권3호
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• pp.177-185
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• 2014

Aspiring to the new raw materials of insecticides is one of the plant extracts. The material structure of a variety of plant extracts have because the material to the defense of the plant itself, the case of insecticides, using plant extracts, safe, has low toxicity and has the advantage of highly distinctive and fall. Coltsfoot (Tussilago farfara L.) is belonging to the family Compositae, and distributed in all parts of China as medicinal plants and indigenous plants used. Coltsfoot is known that it is effect to respiratory disease and has an antiviral effect. However, the reported insecticidal activity of coltsfoot could be not found. Fortunately, I found insecticidal activities when I was screening the bioassay against several insects with a lot of plant extracts. Using the ethanol extract of the Tussilago farfara, there were insecticidal activities against Culex pipiens pallens and Tetranychus urticae. There were several fractions in ethanol extract of coltsfoot by using various organic solvents. Hexane fraction showed a higher insecticidal activity than any other fraction. It is confirmed that hexane fraction contained pyrethrin by using HPLC analysis. So, it might be suggested that extract of coltsfoot has an insecticidal activity and its effect due to a ingredient of pyrethrin.

• Food Science and Biotechnology
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• 제17권2호
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• pp.308-312
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• 2008

The flower buds of Tussilago farfara (TF) have been traditionally used in oriental medicine for the treatment of bronchitis and asthma. In our study, the primary objective was to determine the mechanisms that are inherent to TF-induced cytotoxicity and apoptosis, using the methanolic extract of TF (TFM) in HT-29 human colon cancer cells. We found that TFM-induced induced cytotoxicity in HT-29 cells in a dose-dependent manner. This effect was verified via an MTT reduction assay, an lactate dehydrogenase (LDH) release assay, and a colony formation assay. Interestingly, we also detected apoptotic bodies on Hoechst staining, and attempted to determine whether TFM-induced apoptosis involved the caspase pathway using a caspase-3/7 activity assay. Overall, the results indicate that TFM contain chemotherapeutic agents and potential candidates use for against human colon cancer cells.

• 한국축산식품학회지
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• 제30권2호
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• pp.345-350
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• 2010

Various extracts from 30 medicinal plants were evaluated for their antiviral activity against influenza virus A/Puerto Rico/8/34 (H1N1) and cytotoxicity in MDCK cell culture. The plant material (30 g) was extracted with methanol (300 mL) at room temperature for 24 h, after which the methanolic extracts were filtered, evaporated, and subsequently lyophilized. Evaluation of the potential antiviral activity was conducted by a viral replication inhibition test. Among these medicinal plants, Tussilago farfara, Brassica juncea, Prunus armeniaca, Astragalus membranaceus, Patrinia villosa, and Citrus unshiu showed marked antiviral activity against influenza virus A/H1N1 at concentrations ranging from 0.15625 mg/mL to 1.25 mg/mL, 0.3125 mg/mL to 10 mg/mL, 5 mg/mL to 10 mg/mL, 0.625 mg/mL to 10 mg/mL, 0.625 mg/mL to 10 mg/mL, and 0.3125 mg/mL to 5 mg/mL, respectively. The extracts of Tussilago farfara showed cytotoxicity at concentrations greater than 2.5 mg/mL, whereas the other five main extracts showed no cytotoxicity at concentrations of 10 mg/mL. Taken together, the present results indicated that methanolic extracts of the six main plants might be useful for the treatment of influenza virus H1N1.

• 만화애니메이션 연구
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• 통권39호
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• pp.111-132
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• 2015

로토스코핑 기법은 20세기 초부터 실제 인물의 움직임과 같은 자연스럽고 매끈한 움직임 구사를 위해 오랫동안 활용되어 왔음에도, 이미 촬영된 이미지를 단순히 모방한다는 이유로 예술적 가치가 평가절하 되며 일종의 편법이나 속임수 등으로 여겨져 왔다. 하지만 무한 융합이 가능해진 디지털 시대 속에서 실사와 애니메이션의 경계를 자유롭게 넘나들 수 있는 로토스코핑 기법의 활용은 오히려 리얼리티의 확장적 재현이라는 새로운 미학적 가능성을 제시하고 끊임없이 새로운 애니메이션적 체험의 층위들을 만들어내고 있다. 또한 이러한 로토스코핑의 다양한 융합적 특성들을 적극 활용하고 실험하고 있는 요나스 오델의 작품들은 로토스코핑 이미지가 스타일적인 측면 외에도, 내러티브를 강화하는 수단으로 역할 할 수 있음을 증명할 수 있는 대표적 사례이다. 본 연구는 요나스 오델 감독의 독특한 철학적, 미학적 스타일을 완성시킨 다큐멘터리 형식의 3가지 옴니버스 단편들 -<투실라고 Tussilago>(2010), <거짓말 Lies>(2008), <첫경험 Never like the first time!>(2006) -을 분석하고, 실존 인물의 인터뷰 형식으로 진행되는 내러티브와 실사 이미지와의 구조와 관계 속에서 로토스코핑 이미지들이 어떠한 독특한 역할로 의미 작용을 하는지를 살펴본다. 이는 세 가지 특성- 청각적 감성을 매개하는 내적 리얼리티의 형이상학적 기록으로서의 이미지, 특정 인물에 대한 존재적 표상으로서 익명화된 이미지, 물리적 시공간을 담아내는 기억의 표상으로서 신체 이미지-을 기준으로 분류되고 분석된다. 이를 통해 21세기 디지털 시대에 로토스코핑 이미지는 비단 자연스러운 움직임의 재현과 독특한 스타일을 위한 것을 넘어, 어떠한 새로운 가치들을 획득하게 되는지를 밝힐 것이다. 본 연구가 예술적인 면에서 평가절하 되어왔던 로토스코핑 이미지의 가치를 재발견, 재위치 시킬 수 있는 밑거름으로서 역할하고, 뿐만 아니라 미국, 일본, 혹은 특정 유럽국에 치우친 애니메이션 작가와 스타일 연구의 환경 속에서 세계적으로 주목받고 있는 스웨덴 작가와 그의 독특한 스타일을 새롭게 소개할 수 있는 좋은 계기가 될 수 있기를 기대한다.

• 혜화의학회지
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• 제19권1호
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• pp.49-54
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• 2010

Inhalational drug is an attractive modality for local therapy of pulmonary diseases as well as systemic drug delivery. Flower of Lonicera japonica (FLJ) and flower of Tussilag farfara (FTF) are medicinal herbs for respiratory disease in traditional Korean medicine. As a preliminary study for effective inhalable formulation of FLJ and FTF, this study was to provide the toxicity and anti-inflammatory effect on murine macrophages. The dried FLJ and FTF were extracted with distilled water, filtered and freeze-dried. After treatment with FLJ and FTF extract on RAW 264.7 cells, the cell viabilities were measured by MTT assay. FLJ and FTF did not show cytotoxicity on RAW 264.7 cells. LPS stimulated RAW 264.7 cells were treated with 3 and $30\;{\mu}g/ml$ of FLJ or FTF. FLJ and FTF did not inhibit TNF-a and IL-6 secretion in both concentration of treatment. We suggest that FLJ and FTF may be useful drugs for respiratory disease. Future work will focus on the physical characteristics for inhalable formulation.

• Natural Product Sciences
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• 제11권1호
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• pp.16-21
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• 2005

Nitric Oxide (NO), derived from L-arginine, is produced by two types (constitutive and inducible) of nitric oxide synthase (NOS: cNOS and iNOS). The NO produced in large amounts by the iNOS is known to be responsible for the vasodilation and hypotension observed in septic shock, cancer metastasis and inflammation. The inhibitors of iNOS, thus, may be useful candidates for the treatment of inflammatory diseases accompanied by the overproduction of NO. We prepared alcoholic extracts of herbal drugs which have been used for the treatment of inflammation in oriental medicine. We have screened the inhibitory activity of NO production in lipopolysaccharide (LPS)-activated macrophages after the treatment of these extracts. Among 82 kinds of extracts of herbal drugs, 35 extracts showed the potent inhibitory activity of NO production above 50% at the concentration of $50\;{\mu}g/mL$. The inhibitory activities of NO production were also evaluated for several solvent fractions at two different concentrations. Especially, hexane and EtOAc fractions of Alpinia officinarum, Angelica gigas, Ostericum koreanum, Saussurea lappa, Torilis japonica, and hexane fractions of Agrimonia pilosa, Machilus thunbergii, Hydrangea serrata, Magnolia obovata, Prunella vulgaris, Tussilago farfara, and EtOAC fractions of Perilla frutescence showed a significant activity at 10 and/or $25\;{\mu}g/mL$. In Western blot analysis, the hexane fractions ($5\;{\mu}g/mL$) of Magnolia obovata and Saussurea lappa, and EtOAc fractions ($20\;{\mu}g/mL$) of Hydrangea Serrata, Perilla frutescence and Torilis japonica inhibited the expression of iNOS protein in LPS-activated macrophages. These plants may be promising candidates for the study of the activity-guided purification of active compounds and might be useful for the treatment of inflammatory diseases and endotoxemia accompanying overproduction of NO.

• The Korean Journal of Physiology and Pharmacology
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• 제22권6호
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• pp.671-677
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• 2018

In the present study, we investigated whether tussilagone, a natural product derived from Tussilago farfara, significantly affects the production and gene expression of airway MUC5AC mucin. Confluent NCI-H292 cells were pretreated with tussilagone for 30 min and then stimulated with EGF (epidermal growth factor) or PMA (phorbol 12-myristate 13-acetate) for 24 h or the indicated periods. The MUC5AC mucin gene expression was measured by RT-PCR. Production of MUC5AC mucin protein was measured by ELISA. To elucidate the action mechanism of tussilagone, effect of tussilagone on PMA-induced $NF-{\kappa}B$ signaling pathway was investigated by western blot analysis. Tussilagone significantly inhibited the production of MUC5AC mucin protein and down-regulated the expression of MUC5AC mucin gene, induced by EGF or PMA. Tussilagone inhibited PMA-induced activation (phosphorylation) of inhibitory kappa B kinase (IKK), and thus phosphorylation and degradation of inhibitory kappa Ba ($I{\kappa}B{\alpha}$). Tussilagone inhibited PMA-induced phosphorylation and nuclear translocation of nuclear factor kappa B ($NF-{\kappa}B$) p65. This, in turn, led to the down-regulation of MUC5AC protein production in NCI-H292 cells. These results suggest that tussilagone can regulate the production and gene expression of mucin by acting on airway epithelial cells through regulation of $NF-{\kappa}B$ signaling pathway.