Proceedings of the SCSK Conference (대한화장품학회:학술대회논문집)
- 2003.09b
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- Pages.32-43
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- 2003
The effect of plant extracts on the activity and the expression of MMPs (matrix metalloprotease) induced by UVA
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Lee, Dong-hwan
(R & D Center, Hanbul Cosmetics Co.) ;
- Lee, Bum-chun (R & D Center, Hanbul Cosmetics Co) ;
- Yoon, Eun-jeong (R & D Center, Hanbul Cosmetics Co) ;
- Lee, Kyung-eun (R & D Center, Hanbul Cosmetics Co) ;
- Park, Sung-min (R & D Center, Hanbul Cosmetics Co) ;
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Pyo, Hyeong-bae
(R & D Center, Hanbul Cosmetics Co) ;
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Choe, Tae-boo
(Section of Chemical and Biological Engineering, Konkuk University)
- Published : 2003.09.01
Abstract
UV irradiation on a skin brings about the qualitative and quantitative alterations of the extracellular matrix. Repeated-UV irradiation suppressed the synthesis of collagen and activated the expression of the matrix metalloprotease (MMP). In this paper, on the purpose of development of novel anti-aging agents from natural sources, effects of several natural products on in vitro MMP-1 activity and UVA induced MMP-1 synthesis in human dermal fibroblast (HDF) culture were studied. We measured MMP-1 activities by fluorescence assay using gelatin as substrates. As a result, the extract of Dicentra spectabilis, and flower buds of Tussilago farfara showed strong inhibitory effect. Among them, the extract of flower buds of Tussilago fartara and Dicentra spectabilis inhibited MMP-1 activity by 92% and 87% at 0.05% (w/v). And UVA induced MMP-1 expression were analyzed by enzyme-linked immunosorbent assay (ELISA) and gelatin-based zymography in HDF culture. The extract of flower buds of Tussilago farfara and Dicentra spectabilis suppressed the UVA induced expression of MMP-1 by similar level of Vitamin C 200