Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Cytotoxic and Apoptotic Activities of Tussilago farfara Extract in HT-29 Human Colon Cancer Cells

  • Lee, Mi-Ra (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Cha, Mi-Ran (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Jo, Kyung-Jin (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Yoon, Mi-Young (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
  • Published : 2008.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

The flower buds of Tussilago farfara (TF) have been traditionally used in oriental medicine for the treatment of bronchitis and asthma. In our study, the primary objective was to determine the mechanisms that are inherent to TF-induced cytotoxicity and apoptosis, using the methanolic extract of TF (TFM) in HT-29 human colon cancer cells. We found that TFM-induced induced cytotoxicity in HT-29 cells in a dose-dependent manner. This effect was verified via an MTT reduction assay, an lactate dehydrogenase (LDH) release assay, and a colony formation assay. Interestingly, we also detected apoptotic bodies on Hoechst staining, and attempted to determine whether TFM-induced apoptosis involved the caspase pathway using a caspase-3/7 activity assay. Overall, the results indicate that TFM contain chemotherapeutic agents and potential candidates use for against human colon cancer cells.

Keywords

References

  1. Cha MR, Yoon MY, Kim JY, Hwang JH, Park HR. Induction of cytotoxicity and apoptosis in HT-29 human colon carcinoma cells by a Gleditsiae Semen extracts. Food Sci. Biotechnol. 16: 260-264 (2007)
  2. Kozlenko M, Vol A, Zusman I. Stability-variability conflict as a reason for cancer: Physical and social aspects. Med. Hypotheses 57: 648-654 (2001) https://doi.org/10.1054/mehy.2001.1434
  3. Chattopadhyay S, Bisaria VS, Panda AK, Srivastava AK. Cytotoxicity of in vitro produced podophyllotoxin from Podophyllum hexandrum on human cancer cell line. Nat. Prod. Res. 18: 51-57 (2004) https://doi.org/10.1080/1057563031000122095
  4. Kim EJ, Lee YJ, Shin HK, Park JHY. Induction of apoptosis by the aqueous extract of Rubus coreanum in HT-29 human colon cancer cells. Nutrition 21: 1141-1148 (2005) https://doi.org/10.1016/j.nut.2005.02.012
  5. Park HR, Tomida A, Sato S, Tsukumo Y, Yun J, Yamori T, Hayakawa Y, Tsuruo T, Shin-ya K. Effect on tumor cells of blocking survival response to glucose deprivation. J. Natl. Cancer I. 96: 1300-1310 (2004) https://doi.org/10.1093/jnci/djh243
  6. Chen T, Pengetnze Y, Taylor CC. Src inhibition enhances paclitaxel cytotoxicity in ovarian cancer cells by caspase-9-independent activation of caspase-3. Mol. Cancer Ther. 4: 217-224 (2005)
  7. Rhoo IJ, Park HR, Choo SJ, Hwang JH, Park YM, Bae KH, Shin-ya K, Yoo ID. Selective cytotoxic activity of valinomycin against HT- 29 human colon carcinoma cells via down-regulation of GRP78. Biol. Pharm. Bull. 29: 817-820 (2006) https://doi.org/10.1248/bpb.29.817
  8. Sausville EA. Versipelostain: Unfolding an unsweetened death. J. Natl. Cancer I. 96: 1266-1267 (2004) https://doi.org/10.1093/jnci/djh276
  9. Lim SM, Lee GJ, Park SM, Ahn DH, Im DS. Chracterization of Lactobacillus cellobiosus D37 isolated from soybean paste as a probiotic with anticancer and antimicrobial properties. Food Sci. Biotechnol. 15: 792-798 (2006)
  10. Volate SR, Davenport DM, Muga SJ, Wargovich MJ. Modulation of aberrant crypt foci and apoptosis by dietary herbal supplements (quercetin, curcumin, silymarin, ginseng, and rutin). Carcinogenesis 26: 1450-1456 (2005) https://doi.org/10.1093/carcin/bgi089
  11. Huerta S, Arteaga JR, Irwin RW, Ikezoe T, Heber D, Koeffler HP. PC-SPES inhibits colon cancer growth in vitro and in vivo. Cancer Res. 62: 5204-5209 (2002)
  12. Eisenberg DM, Kessler RC, Foster C, Norlock FE, Calkins DR, Delbanco TL. Unconventional medicine in the United States. Prevalence, costs, and patterns of use. New Engl. J. Med. 328: 246-252 (1993) https://doi.org/10.1056/NEJM199301283280406
  13. Risberg T, Lund E, Wist E, Kaasa S, Wilsgaard T. Cancer patients use of nonproven therapy: A 5-year follow-up study. J. Clin. Oncol. 16: 6-12 (1998) https://doi.org/10.1200/JCO.1998.16.1.6
  14. Gouaze V, Yu JY, Bleicher RJ, Han TY, Liu YY, Wang H, Gottesman MM, Bitterman A, Giuliano AE, Catbot MC. Overexpression of glucosylceramide synthase and P-glycoprotein in cancer cells selected for resistance to natural product chemotherapy. Mol. Cancer Ther. 3: 633-639 (2004)
  15. Oh CH, Kang PS, Kim JW, Kwon J, Oh SH. Water extracts of cultured mountain ginseng stimulate immune cells and inhibit cancer cell proliferation. Food Sci. Biotechnol. 15: 369-373 (2006)
  16. Hong J, Yang CS. Effect of purified green tea catechins on cytosolic phospholipase A2 and arachidonic acid release in human grastointestinal cancer cell lines. Food Sci. Biotechnol. 15: 799-804 (2006)
  17. Kodach LL, Bos CL, Duran N, Peppelenbosch MP, Ferreira CV, Hardwick JCH. Violacein synergistically increases 5-fluoruracill cytotoxicity, induces apoptosis, and inhibits Akt-mediated signal transduction in human colorectal cancer cells. Carcinogenesis 27: 508-516 (2006) https://doi.org/10.1093/carcin/bgi307
  18. Hong KJ, Dunn DM, Shen CL, Pence BC. Effects of Ganoderma lucidum on apoptotic and anti-inflammatory function in HT-29 human colonic carcinoma cells. Phytother. Res. 18: 768-770 (2004) https://doi.org/10.1002/ptr.1495
  19. Namba T. The Encyclopedia of Wakan-Yaku. Vol. II. Hoikusha, Tokyo, Japan. p. 525 (1980)
  20. Cho JS, Kim HY, Ryu JH, Jeong YS, Lee YS, Jin CB. Neuroprotective and antioxidant effects of the ethyl acetate fraction prepared from Tussilago farfara L. Biol. Pharm. Bull. 28: 455-460 (2005) https://doi.org/10.1248/bpb.28.455
  21. Hwang SB, Chang MN, Garcia ML, Han QQ, Huang L, King VF, Kaczorowski GJ, Winquist RJ. L-652,469-a dual receptor antagonist of platelet activating factor and dihydropyridines from Tussilago farfara L. Eur. J. Pharmacol. 141: 269-281 (1987) https://doi.org/10.1016/0014-2999(87)90272-X
  22. Kokoska L, Polesny Z, Rada V, Nepovim A, Vanek T. Screening of some Siberian medicinal plants for antimicrobial activity. J. Ethnopharmacol. 82: 51-53 (2002) https://doi.org/10.1016/S0378-8741(02)00143-5
  23. Ryu JH, Jeong YS, Sohn DH. A new bisabolene epoxide from Tussilago farfara, and inhibition of nitric oxide synthesis in LPSactivated macrophages. J. Nat. Prod. 62: 1437-1438 (1999) https://doi.org/10.1021/np990116c
  24. Kim MR, Lee JY, Lee HY, Aryal DK, Kim YG, Kim SK, Woo ER, Kang KW. Antioxidative effects of quercetin-glycosides isolated from the flower budes of Tussilago farfara L. Food Chem. Toxicol. 44: 1299-1307 (2006) https://doi.org/10.1016/j.fct.2006.02.007
  25. Huang Q, Deveraux QL, Maeda S, Salvesen GS, Stennicke HR, Hammock BD, Reed JC. Evolutionary conservation of apoptosis mechanisms: Lepidopteran and baculoviral inhibitor of apoptosis proteins are inhibitors of mammalian caspase-9. P. Natl. Acad. Sci. USA 97: 1427-1432 (2000) https://doi.org/10.1073/pnas.97.4.1427
  26. Suzuki H, Tomida A, Tsuruo T. Dephosphorylated hypoxia-inducible factor 1a as a mediator of p53-dependent apoptosis during hypoxia. Oncogene 20: 5779-5788 (2001) https://doi.org/10.1038/sj.onc.1204742
  27. Ogiso Y, Tomida A, Lei S, Omura S, Tsuruo T. Proteasome inhibition circumvents solid tumor resistance to topoisomerase - directed drugs. Cancer Res. 60: 2429-2434 (2000)
  28. Lee H, Kim YJ, Kim HW, Lee DH, Sung MK, Park T. Induction of apoptosis by Cordyceps militaris through activation of caspase-3 in leukemia HL-60 cells. Biol. Pharm. Bull. 29: 670-674 (2006) https://doi.org/10.1248/bpb.29.670