Browse > Article

Effects of aqueous extracts from Lonicera japonica and Tussilago farfara on RAW 264.7 Macrophages  

Lee, Eung-Seok (Div. of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Daejeon University)
Yang, Su-Young (Div. of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Daejeon University)
Park, Yang-Chun (Div. of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Daejeon University)
Oh, Young-Seon (Hyehwa Hostpital, Daejeon University)
Lee, Jin-Woo (Hyehwa Hostpital, Daejeon University)
Lee, Yong-Koo (Hyehwa Hostpital, Daejeon University)
Publication Information
Journal of Haehwa Medicine / v.19, no.1, 2010 , pp. 49-54 More about this Journal
Abstract
Inhalational drug is an attractive modality for local therapy of pulmonary diseases as well as systemic drug delivery. Flower of Lonicera japonica (FLJ) and flower of Tussilag farfara (FTF) are medicinal herbs for respiratory disease in traditional Korean medicine. As a preliminary study for effective inhalable formulation of FLJ and FTF, this study was to provide the toxicity and anti-inflammatory effect on murine macrophages. The dried FLJ and FTF were extracted with distilled water, filtered and freeze-dried. After treatment with FLJ and FTF extract on RAW 264.7 cells, the cell viabilities were measured by MTT assay. FLJ and FTF did not show cytotoxicity on RAW 264.7 cells. LPS stimulated RAW 264.7 cells were treated with 3 and $30\;{\mu}g/ml$ of FLJ or FTF. FLJ and FTF did not inhibit TNF-a and IL-6 secretion in both concentration of treatment. We suggest that FLJ and FTF may be useful drugs for respiratory disease. Future work will focus on the physical characteristics for inhalable formulation.
Keywords
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Tayab ZR, Hochhaus G. Pharmacokinetic/pharmacodynamic evaluation of inhalation drugs: application to targeted pulmonary delivery systems. Expert Opin Drug Deliv. 2005;2(3):519-32.   DOI   ScienceOn
2 Han JH, Kang SY, Yu KS, Jin SS, Ha KH, Lee KJ. Farfarea Flos inhitits histamine-induced contractile reponses of airway smooth muscle. Korean J Oriental Int Med, 17(1):210-7, 1996.
3 Kwak SK, Choi SM, Park YC. Effect of Farfarae Flos extract drug on immune cells and cytokines in BALF of OVA-induced asthmatic mice. Korean J Oriental Pathol & Physiol, 19(3):716-21.
4 Choi HJ, Bang NY, Song BW, Kim NJ, Rhyu BH. Survey on the preference for the dosage forms of oriental herbal medicine. Kyunghee Medicine, 20(1): 46-57, 2004.
5 Hillery AM, Lloyd AW, Swarbrick J. Drug delivery and targeting; for pharmacists and pharmaceutical scientists. London, Taylor and Francis, p2, 2001.
6 Derendorf H, Nave R, Drollmann A, Cerasoli F, Wurst W. Relevance of pharmacokinetics and pharmacodynamics of inhaled corticosteroids to asthma. Eur Respir J, 28(5):1042-50, 2006.   DOI   ScienceOn
7 Yun YG, Kim GM, Lee SJ, Ryu SH, Jang SI. Inhibitory effect of aqueous extract from Lonicera japonica flower on LPS-induced inflammatory mediators in RAW 264.7 macrophages. Korea J Herbology, 22(3):117-25, 2007.   과학기술학회마을
8 Lee HJ, Kim JS, Jina C, Ryu JH. Inhibitory activity of medicinal herbs on nitric oxide synthesis in activated macrophages. Natural Product Sciences. 11(1):16-21, 2005.   과학기술학회마을
9 Hwangbo C, Lee HS, Park J, Choe J, Lee JH. The anti-inflammatory effect of tussilagone, from Tussilago farfara, is mediated by the induction of heme oxygenase-1 in murine macrophages. Int Immunopharmacol. 9(13-14):1578-84, 2009.   DOI   ScienceOn
10 Cho JS, Kim HY, Ryu JH, Jeong YS, Lee YS, Jin CB. Neuro-protective and antioxidant effects of the ethyl acetate fraction prepared from Tussilag farfara L. Biol Pharm Bull, 28:455-60, 2005.   DOI   ScienceOn
11 Lee DE, Lee JR, Kim YW, Kwon YK, Byun SH, Shin SW et al. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-a, IL-1b and COX-2 expression by flower and whole plant of Lonicera japonica. Korean J Oriental Physiol & Pathol, 19(2):481-9, 2005.
12 Suhr SS, Jung SK. Antiviral effects of fermented Lonicerae Flos on A type influenza virus. Korean J Oriental Int Med, 30(3):465-80, 2009.   과학기술학회마을
13 Kim YD. Effects of lactobacillus fermented flos lonicerae on the murine model of chronic obstructive pulmonary disease. Seoul, Kyunghee Univ., 2009.
14 Lee JS, Jung HJ, Jung SK, Rhee HK. The effects of Lonicerae Flos on bleomycin-induced lung fibrosis. Korean J Oriental Int Med, 25(1):71-80, 2004.   과학기술학회마을
15 Namba T. The encyclopedia of Wakan-Yaku (Vol. 2). Tokyo, Hoikusha, p525, 1980.
16 Jung HJ, Park SK, Jung SK, Rhee HK. The inhibitory effects of Lonicerae Flos and Paeoniae Radix on IL-6, IL-16, GM-CSF mRNA level by BEAS-2B human epithelial cells. J Korean Oriental Med, 24(3):145-54, 2003.   과학기술학회마을
17 Chung KJ, Jung HJ, Jung SK, Rhee HK. Lonicerae Flos contributes to the chemotaxis of eosinophils and secretion of cytokines in A549 human epithelial cells. Korean J Oriental Int Med, 26(1):129-42, 2005.   과학기술학회마을
18 Kim SH, Lee KH, Lee YJ, An DK, Lee SI, Seo YB, et al. Herbalogy. 3rd ed. Seoul, Younglimsa, pp198-9, 482-3, 1995.
19 Lee DJ. Effects of Lonicera japonica on the murine model of chronic obstructive pulmonary disease. Seoul, Kyunghee Univ., 2009.