• Korean Journal of Plant Resources
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• v.10 no.3
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• pp.213-220
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• 1997

The present study has been undertaken to characterize availability of citrus as a medicinal plant with antineoplastic property. The crude extracts from 40 species of fruits with 12 species of the local Citrus in Cheju island were evaluated on their potential activities against mouse P388 lymphocytic leukaemia in vitro. The percent cytotoxicity varied from 25.40 to 97.94% at a concentration of $100{\mu}g/mL$. Among 40 spp., 8 species showed high toxicity more than 90% against P388 cells and Cheongkyool(C. nippokoreana) exhibited the most cytotoxicity as 97.94%($IC_{50}=20.2{\mu}g/mL$). Nine varieties of C. junos were showed insiginicant cytotoxicity. In trifoliate orange, immature fruit was stronger than mature and peel extract showed higher cytotoxicity($IC_{50}=18{\mu}g/mL$) than the other tissues. Hexane fraction from methanol(MeOH) extract of trifoliate orange showed highly significant inhibition of cell growth($IC_{50}=3.9{\mu}g/mL$). In addition, its cytotoxicity increased remarkably from 3.95 to $0.40{\mu}g/mL$ as exposure time legthened. Cytotoxic activities of crude extracts were decreased considerably during a six months storage period. It was apparent that there is considerable variation in cytotoxicity, depending upon species, maturity and storage time of extracts. There was no meaningful cytotoxic difference between archicitrus and metacitrus in the genus Citrus.

• Journal of the Korea Society of Computer and Information
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• v.26 no.12
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• pp.237-245
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• 2021

This study investigated the Antimicrobial activity of Rutaceae fruit ethanol extracts against microorganisms-induced skin inflammation in cosmetic materials. Rutaceae fruits were separated in two parts of whole fruit (pulp, pulp fegment membrane, peel) and peel, and extracted with 70% ehtanol. The results demonstrated that Rutaceae fruit ethanol extracts showed antimicrobial activity in 5 strains except Staphylococcus aureus. In particular, the antimicrobial activity against Staphylococcus epidermidis was the best in fresh lemons whole fruit. The antimicrobial activity against Escherichia coli was shown only in fresh lemon peel and fresh trifoliate peel. Additionally, antimicrobial activity against Propionibacterium acnes was shown only in the dried lemon peel. In the results of antimicrobial activity against Pityrosporum ovale, in the case of fresh fruits, citron whole fruits showed the highest effect, followed by lemon whole fruits and mandarin orange peel. And in the case of dried fruits, orange peel showed the highest effect, followed by trifoliate peel, mandarin orange peel and lemon peel. Therefore, it is considered that lemon, which shows antimicrobial activity against all skin inflammation-causing microorganisms, can be used as a natural material for improving skin inflammation in cosmetics.

• Korean journal of applied entomology
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• v.9 no.1
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• pp.33-35
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• 1970

The author collected 2 species of spider mites from persimmon and citrus trees in Taegu and Chun Chon during the summer of 1961 and 1969. Tenuipalpus japonicus collected from persimmon trees and Panonychus citri collected from citrus trees in and from trifoliate orange trees. In 1958 and 1961 Dr. E. S. Lee recorded four phytophagous species, i.e. Panonychus ulmi, Tetranychus pacificus, Tetranychus urticae and Bryobia praetiosa.

• Journal of Life Science
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• v.23 no.9
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• pp.1079-1087
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• 2013

Speed germination success and robust vegetative growth of citrus rootstock through improved sowing methods and fertilizer inputs offer the usage of root system for the citrus. The current study evaluated the influence of seed coat removal and different fertilizer concentrations on plant germination and plant growth of spontaneous rootstock siblings. Decoated and coated seeds of diploid and tetraploid plants were sown in tubes. Commercial fertilizer concentrations of 0, 2, 4, 6, 8 and $10g{\cdot}l^{-1}$ were added. The experimental layout followed a randomized block $2{\times}6$ factorial design (seed coat removal ${\times}$ fertilizer concentration) for each rootstock. Fertilizer concentrations were 0, 10, 20 and $30g{\cdot}l^{-1}$ of the fertilizer for the resistance of the strength on the salt level. The germination rate of seeds without testa sown in vitro was improved (67-80%) compared to that of nontreated seeds. The eventual tree height of the seeds without testa in the diploid group was increased due to higher fertilization compared to that in the nontreated group. The removal of seed testa promoted the seed germination of both diploid and tetraploid trifoliate orange and resulted in greater height. Their vegetative development was also increased due to the increased fertilization of the rootstock. The Fv/Fm value for the diploid plants was 0.4 and 0.8 for the tetraploid ones under salt stress after 11 days of treatment. The removal of seed testa may improve the seed germination of trifoliate orange. Tetraploid trifoliate orange appears to possess resistance to salt stress compared to the diploid variety.

• Korean journal of applied entomology
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• v.3
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• pp.31-39
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• 1964

The authors studied on the bionomics of the Florida wax scale, Ceroplastes floridensis Comstock and bionomics of two kind of parasites which attack on this Florida wax scale in 1963 to 1964. 1. Ceroplastes floridensis Comstock was heavily infested on the persimmon tree planted in the southern part of Korea, specially at the center of Taegu city. Parasitic ratio of important parasite of Ceroplastes floridensis Comstock, Microterys clauseni was $66.67\%$ and appeared low parasited ratio at the center of the city. 2. The developed eggs of the Ceroplastes floridensis Comstock was appear며 from mid-May to early July. The peak of it was late-May to early June. Average stored eggs inside of a body is 2030. $2030.77\pm0.49.$. 3. The hatching period of the Ceroplastes floridensis Comstock begins from early June to early July. Mid-June was the peak period of it. Pupation period of the male was August 14 to September 7 and adult was emerged on August 20 to September 8. Mean longevity for adult male was 3 days in August but 4 days in September. 4. On the differences of the body- length parasited on the three host plants, persimmon, platanus, and trifoliate orange, the body length parasited on the persimmon was the largest, medium on platanus and the smallest on the trifoliate orange. 5. Microterys clauseni H. Compere parasite on tile Ceroplastes floridensis Comstock with 5 generations in a year. Hibernate in the body of the host as tan·at stage. The first adult appears in middle April. Fifteen days was required for one generation in $21^{\circ}$. The authors observed on the form ovary, female with 6 ovarioles, and mean number of eggs per female was $47.19\pm0.55$. 6. On the parasited individuals of the Microterys clauseni H. Compere a larva was the most numerous and was found 2 individuals or 4 individuals parasite on the one Ceroplastes floridensis Comstock. 7. Coccophagus app. parasited on the only male body of the Ceroplastes floridensis Comstock. The parasitic ratio was $14.831\%$ on persimmon tree in Taegu city.

• Journal of the Korean Applied Science and Technology
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• v.28 no.2
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• pp.196-202
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• 2011

Coumarin derivatives were shown to possess valuable pharmacological properties such as anticancer/anti carcinogenic, anti-inflammatory, anti helicobacter, anti genotoxic, neuroprotective and dietary effect. In this study, novel coumarin derivatives structurally related to 7-geranyloxycoumarin were effectively synthesised in good yields by $Cs_2CO_3$/acetonitrile in mild condition. The synthesis of geranyloxycoumarin derivatives in weak base($Na_2CO_3$, $K_2CO_3$, $Cs_2CO_3$ etc)/$CH_3CN$ at room temperature obtained in good yield. On the other hand, the reaction of geranyloxycoumarin formation in strong base(NaOH, KOH, CsOH etc)/$CH_3CN$ at reflux condition obtained in low yield.

• Korean Journal of Plant Resources
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• v.31 no.6
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• pp.684-694
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• 2018

This study describes the successful establishment of a cryopreservation protocol for Citrus limon cultivars: 'Frost Eureka limon' and 'Cook Eureka limon', using a droplet-vitrification method. The shoot tips that were excised from in vitro grown seedlings of the two cultivars were preserved in liquid nitrogen (LN) and successfully regenerated into whole plants. Excised shoot tips were pre-cultured for 1 or 2 days in 0.3 M and 0.5 M sucrose solutions at $25^{\circ}C$ and incubated in a loading solution (LS) composed of 17.5% glycerol + 17.5% sucrose in Murashige and Skoog (MS) medium for 40 min at $25^{\circ}C$. Prior to direct immersion in LN for 1 h, the shoot tips were dehydrated with plant vitrification solution 2 (PVS2) at $0^{\circ}C$ or PVS3 at $25^{\circ}C$. The frozen shoot tips were re-warmed and unloaded with 1.2 M sucrose in $\text\tiny{^1/_2}$ MS for 30 min at $25^{\circ}C$. Shoot tips were post-cultured overnight on survival medium and then micrografted onto 'trifoliate orange' (Poncirus trifoliate (L.) Raf. seedling rootstocks for recovery and to produce whole plants. The highest regrowth rates were 53.5% and 50.3% for cryopreserved shoot tips of 'Frost Eureka limon' and 'Cook Eureka limon', respectively, when pre-cultured in 0.3 M and 0.5 M sucrose concentrations in a sequencing manner, with LS and treated with PVS2 for 60 min at $0^{\circ}C$. We also investigated whether the ammonium ion concentration on post-culture medium affected the viability of the cryopreserved Citrus shoot tips. The viability of cooled samples, following culturing on woody plant media (WPM) containing $\text\tiny{^1/_4}$ ammonium nitrate overnight before micrografting, was the highest (70.3%) in 'Frost Eureka limon'. The study described here is a cost-effective and safe method to conserve Citrus fruit cultivars, for the improvement and large-scale multiplication of fruit plants and for breeding disease resistance.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.41 no.1
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• pp.35-46
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• 2023

This study investigated and analyzed ancient records on the type, planting background, and construction process of Gwanbang forest(關防林) planned for military defense during the Joseon Dynasty to find out the purpose, location, and planting species of Gwanbang forest. The research results were as follows. During the Joseon Dynasty, Gwanbang forests were created around various government facilities(關防施設), such as Eupseong(邑城), major government offices, camps, and fortifications, for the purpose of defending against enemies. Gwanbang forest includes Yeongaeglim(嶺阨林), which was created on the crest of a strategically important hill, and Military Forest created for military purposes. Most of the spirit forest was designated as Geumsan(禁山) and protected and managed, and the Gwanbang forest was created for various purposes such as shielding, flood damage and river bank erosion prevention as well as external defense. In addition, in order to continuously and efficiently produce wood, which is a material for ships, buildings, and agricultural tools, in most cases, large areas were created as mixed forests. As for the species constituting the Gwanbang forest, there are records of tangerine tree, which is effective for defense because it has thorns, and deciduous broad-leaved trees such as zelkova, elm, willow, david hemiptelea, and oak appear. In the case of Ganghwa island, which served as the defense of the capital and the royal family during the Joseon Dynasty, several records have confirmed that a forest densely planted with trifoliate orange was created for the purpose of Gwanbang forest to reinforce the defense of the outer fortress. Based on historical research in the literature, assuming that the natural monument 'Gapgotri tangerine tree in Ganghwa Island' was planted in the 30th year of King Sukjong(1704), the first record of planting trifoliate orange in Ganghwa Island, the maximum age is estimated to be more than 319 years.

• Korean Journal of Environmental Agriculture
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• v.19 no.3
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• pp.206-212
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• 2000

The effects of two phosphorus sources (fused phosphate and rock phosphate), applied at different rates, on growth, arbuscular-mycorrhizae(AM) formation in roots and nutrient contents of trifoliate orange grown in an uncultivated volcanic ash soil were investigated in a greenhouse. The seedlings were either inoculated with AM fungi or left uninnoculated. Growth of seedlings were best in the treatments of 156-272 mg P/kg with fused phosphate. Although the applied P in the rack phosphate treatments were nearly same or much higher comparing to the fused phosphate treatments, seedling growth were significantly less. Soil available P in the treatment of 272 mg P/kg of fused phosphate was maintained in the range of 3-5 mg/kg during the experiment, and the AM formation was about 60% in average. In the treatments of lower rates of fused phosphate application or of rock phosphate application, soil available P were lower than 3 mg P/kg and AM formations were less than 30%. Significant increases were found in seedling growth and nutrient absorption due to AM fungi inoculation, and the effects were much more significant in the treatments of higher AM formation. In most of citrus groves in Cheju island, soil available P is much higher than 200 mg P/kg, and average AM formation in citrus roots is less than 30%. Results obtained in this study show that the formation of AM can be increased at much lower level of available P than the present levels found in citrus groves.

• Plant Resources
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• v.5 no.1
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• pp.29-44
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• 2002

In this study, plant regeneration through in vitro culture from plantlet stems of Yooja (C. junos Sieb.) and trifoliate orange (P. trifoliata Rafin.) was attempted to make mass-production system of virus-free plants having the same genotype with mother plant. In order to investigate physiological change depending on the developmental stage of plant regeneration, the changes of total protein, peroxidase and esterase activity and their isozyme patterns as well were examined in 1/2 MS medium. The results are as follows : 1. The MS medium for the optimal callus induction and shoot formation was utilized. The medium was supplemented either with 2,4-D and Kinetin or with BA and NAA. The optimal concentrations were the combination of 1.0mg/ 2,4-D +0.3mg/ Kinetin and 1.0mg BA +0.3mg NAA in callus induction and shoot formation, respectively. 2. For the plant regeneration from somatic embryos, 1/2 MS medium was used with supplements of growth regulators (free, 1.0mg/ IBA +1.0mg/ BA ,0.5mg/ IBA +0.5mg/ BA). Shooting and rooting were the best in the treatment of 0.5mg/ IBA and 0.5mg/ BA combination. 3. The total protein content has a tendency of increase with the developmental stage of embryo, but it was decreased at the plantlet. Also it was the highest at 8 and 6 weeks stage in C. junos Sieb. and P. trioliata Rafin, respectively. In the SDS-PAGE pattern of protein, C. junos Sieb. showed bands of 29.0 and 40kDa at 10 weeks. The 45,66 and 97.4 kDa bands at 10 weeks of culture were shown in P. trifoliata Rafin. 4. The highest esterase activity was shown at the 6 and 8 weeks of culture in C.junos Sieb. and P. trifoliata Rafin.., respectively. 5. Esterase isozyme patterns were shown difference according to the developmental stage. In C. junos Sieb. a new band was observed at pl 7.7 following 4 weeks culture. On the other hand, new bands in P. trifoliata Rafin. were observed at pl 7.5~6.5 following 4 and 6 weeks culture, respectively.