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http://dx.doi.org/10.5352/JLS.2013.23.9.1079

Improvement of Seed Germination in a Spontaneous Autotetraploid of Poncirus and Chlorophyll Fluorescence of Seedlings in Salt Stress  

Chae, Chi Won (Citrus Research Station, NHRI, RDA)
Yun, Su Hyun (Citrus Research Station, NHRI, RDA)
Park, Jae Ho (Citrus Research Station, NHRI, RDA)
Kim, Min Ju (Citrus Research Station, NHRI, RDA)
Han, Seung Gab (Citrus Research Station, NHRI, RDA)
Kang, Seok Beom (Citrus Research Station, NHRI, RDA)
Koh, Sang Wook (Citrus Research Station, NHRI, RDA)
Han, Sang Heon (Faculty of Bioscience and industry, College of Applied Life Sciences, Jeju National University)
Publication Information
Journal of Life Science / v.23, no.9, 2013 , pp. 1079-1087 More about this Journal
Abstract
Speed germination success and robust vegetative growth of citrus rootstock through improved sowing methods and fertilizer inputs offer the usage of root system for the citrus. The current study evaluated the influence of seed coat removal and different fertilizer concentrations on plant germination and plant growth of spontaneous rootstock siblings. Decoated and coated seeds of diploid and tetraploid plants were sown in tubes. Commercial fertilizer concentrations of 0, 2, 4, 6, 8 and $10g{\cdot}l^{-1}$ were added. The experimental layout followed a randomized block $2{\times}6$ factorial design (seed coat removal ${\times}$ fertilizer concentration) for each rootstock. Fertilizer concentrations were 0, 10, 20 and $30g{\cdot}l^{-1}$ of the fertilizer for the resistance of the strength on the salt level. The germination rate of seeds without testa sown in vitro was improved (67-80%) compared to that of nontreated seeds. The eventual tree height of the seeds without testa in the diploid group was increased due to higher fertilization compared to that in the nontreated group. The removal of seed testa promoted the seed germination of both diploid and tetraploid trifoliate orange and resulted in greater height. Their vegetative development was also increased due to the increased fertilization of the rootstock. The Fv/Fm value for the diploid plants was 0.4 and 0.8 for the tetraploid ones under salt stress after 11 days of treatment. The removal of seed testa may improve the seed germination of trifoliate orange. Tetraploid trifoliate orange appears to possess resistance to salt stress compared to the diploid variety.
Keywords
Fv/Fm; germination rate; sulfuric acid; testa treatment; trifoliate orange;
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