• Journal of Plant Biotechnology
• /
• 제41권1호
• /
• pp.10-18
• /
• 2014

식물지방은 인간의 필수지방산과 영양 공급원 이외에 산업원료와 바이오 연료로 사용되고 있다. 식물지방은 색소체에서 지방산이 합성되어 소포체로 이동되어 최종적으로 중성지방으로 합성 축적되는 복잡한 대사과정을 거친다. 최근 연구결과에 의하면 종자지방 합성에 있어서 기존에 알려진 케네디 경로 이외에 세포막의 주요 인지질인 PC가 종자지방 합성에 있어 중요한 역할을 하며 종자지방에 존재하는 지방산의 불포화도를 조절하는데 핵심 역할을 함이 밝혀졌다. 또한 색소체로부터 소포체로의 지방산 이동에 PC가 직접적인 운반체 역할을 할 가능성이 제시되고 있다. PC가 종자지방 합성에 중요한 역할을 한다는 결과는 앞으로 PC가 어떤 기작에 의해 지방산을 종자로 이동시키며 소포체의 어느 특정부위에서 지방을 합성하는지에 대한 연구가 이루어져야 한다.

• 한국응용과학기술학회지
• /
• 제12권1호
• /
• pp.119-124
• /
• 1995

Conventional alkaline catalytic procedure, including sodium methoxide-methanol N, N, N', N'-tetramethyl guanidine-methanol, and acid-catalytic methods of $BF_{3}-methanol$ and HCI-methanol, have been applied for preparing methyl esters from the triacylglycerols of Trichosanthes kirilowiil seeds containing conjugated trienoic acids. The alkaline catalytic methods produce the methyl esters quantitatively without isomerization of the conjugated trienoic acids, but the acid-catalytic ones destroy almost the molecules of conjugated trienoic acids during transesterification of the triacylglycerols although the molar ratios of monoenoic and dienoic acids (non-conjugated) to saturated acids are in good agreement with those obtained from the alkaline methods.

• Journal of Plant Biotechnology
• /
• 제41권3호
• /
• pp.107-115
• /
• 2014

식물지방은 주로 종자에서 생산되는데 인류에게 필수 지방산을 공급하는 식품 뿐 아니라 바이오디젤 등 산업원료로 그 이용가치가 크다. 식물지방의 수요 증가에 따른 식물지방의 생산증대가 필요하다. 식물지방을 종자 이외의 바이오매스가 큰 식물의 잎에서 생산한다면 식물지방 생산 증진이 가능할 것이다. 잎은 지방을 생산하는 기관이 아니며 주로 광합성을 통해 탄소를 고정하여 다른 기관으로 탄소를 공급하는 기능을 하고 있어 지방을 생산 축적하는 기관으로 전환하는 데는 많은 고려가 필요하다. 그럼에도 불구하고 최근 지방합성 조절인자인 WRI 유전자, 지방을 생성하는 acyltransferase인 DGAT 유전자의 발현에 의해 잎에서 지방을 합성할 수 있었다. 또한 지방의 분해를 안정화하는 올레오신 단백질의 추가 도입으로 잎에서 건조중량당 15%의 중성지방 생산을 보여 잎에서 지방생산 가능성을 보여주었다(Vanhercke et al. 2014). 앞으로 바이오매스에서 지방을 생산하는 연구가 활발할 것으로 예측되며 이 기술을 식용작물이 아닌 비식용이며 바이오매스가 큰 거대억새 등에 도입하여 농지로 적합하지 않은 열악한 토지 및 간척지 등에 재배하여 실용화한다면 미래 지속 생산 가능 친환경 바이오 원료 생산 자원으로 사용 가능하리라 사료된다.

• 한국응용과학기술학회지
• /
• 제4권1호
• /
• pp.77-81
• /
• 1987

No difference was observed on autoxidative stability of triacylglycerols before and after randomyl interesterification. It indicate that randomization of the glyceride composition has no significant effect on the autoxidative stability. On the other hand, the autoxidation of randomyl interesterified vegetable oil was accelerated, caused mainly by decrease of tocopherols during the series of interesterification procedures.

• BMB Reports
• /
• 제33권6호
• /
• pp.499-505
• /
• 2000

The level of long-chain n-3 fatty acids in chicken and pork can be increased by changing the diet of the animals. Increased levels of these essential fatty acids improve cardiovascular health in humans. The purpose of this study was to study the effects of the consumption of pork and chicken enriched in docosahexaenoic acid (DHA) on plasma lipids. The consumption of these products decreased the levels of two cardiovascular risk factors, LDL-cholesterol and triacylglycerols, in the plasma of female college students. The effect on LDL-cholesterol differed from that of fish oil, which does not affect the level of LDL-cholesterol. The proportions of DHA in the triacylglycerols and the glycerophospholipids were increased markedly. The greatest changes in the glycerophospholipids were in the ether types of the ethanolamine glycerophospholipids. Dietary DHA appears to be incorporated preferentially into the plasma ethanolamine plasmalogens, which can act as antioxidants. This agrees with our hypothesis that DHA stimulated the transcription of the genes for peroxisomal enzymes that are required for plasmalogen synthesis.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2000년도 춘계학술발표대회
• /
• pp.29-32
• /
• 2000

• 미생물학회지
• /
• 제30권3호
• /
• pp.171-186
• /
• 1992

Lipolytic characteristics of candia cylindracea lipase was studied by various triacylglycerols with different fatty acyl chains as substrate. The substrate was emulsified with gum arabic and the rate of hydrolysis was determined by pH stat method. The effects of gum concentration, pH, temperature, and $Ca^{2+}$ ion on the enzyme activities were examined. The results show that the effect of these factors are markedly depending on the structurla nature of substrates. The triolein was the best substrate among tested. Present study demonstrates that for characterization of lipolytic enzymes, it is critically important to select proper substrate and activator.r.

• 한국응용과학기술학회지
• /
• 제10권1호
• /
• pp.1-8
• /
• 1993

The possibilities for HPLC analysis of lipids have been revolutionised by the availability of evaporative light-scattering detectors, with which the response is independent of the nature of the mobile phase and does not depend On the presence of specific chromophores in the lipids. It was thus possible to develop an HPLC procedure, involving ternary gradient elution, for separating all the lipid classes in animal tissues in a single step. Although reversed-phase HPLC has been widely used for the analysis of molecular species of lipids, sliver ion chromatography can be a valuable alternative. For example, a stable silver ion column for HPLC was developed which permitted resolution of molecular species of triacylglycerols, even from such complex samples as fish oils, again With light-scattering detection and gradient elution. The capacity for HPLC resolution of diastereomeric diacyl-sn-glycerol derivatives, prepared from triacylglycerols. has lead to a new simple method for stereospecific analysis of the latter.

• 한국물환경학회지
• /
• 제31권2호
• /
• pp.134-141
• /
• 2015

The focus of this study was to observe the growth of Chlorella vulgaris and Botryococcus braunii under mixotrophic conditions (i.e., added acorn) with the aim of increasing biomass and triacylglycerols (TAGs) content. The result of investigation indicated that the acorn contains a lot of carbonate (87.29%) and glucose (97.99 mg%). A significant growth of biomass was obtained when grown in acorn rich environment comparing to autotrophic conditions. 3 g/L acorn yielded the highest biomass concentration for these strains. Thus, the biomass productivity with 3 g/L acorn was obtained 2.31 times and 2.10 times higher than that of authotrophic conditions for Chlorella vulgaris and Botryococcus braunii, respectively. The maximum amount of TAGs was reached 14.35% and 18.41% for Chlorella vulgaris and Botryococcus braunii, respectively, in the growth medium with 5 g/L acorn. The effect of acorn could enhance the investigated microalgae growth, biomass productivity and TAGs content. This provides a feasible way to reduce the cost of bioenergy production from microalgae.

• Bulletin of the Korean Chemical Society
• /
• 제24권4호
• /
• pp.527-530
• /
• 2003