• Molecules and Cells
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• v.21 no.3
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• pp.356-359
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• 2006

The transcription factor E2F1 coordinates cell cycle progression and induces apoptosis in response to DNA damage stress. Aside from DNA damage, the role of E2F1 in the endoplasmic reticulum (ER) stress signaling pathways is unclear. We found that $E2F1^{-/-}$ murine embryonic fibroblasts (MEFs) are resistant to apoptosis triggered by the ER stress inducer thapsigargin. In addition, E2F1 deficiency results in enhanced phosphorylation of eukaryotic translation initiation factor $2{\alpha}$ ($elF2{\alpha}$). These results therefore indicate that E2F1 deficiency increases phosphorylation of $elF2{\alpha}$ in response to ER stress triggered by thapsigargin, and suggest that the reduction in ER stress-induced apoptosis in E2F1-deficient cells is related to the high level of $elF2{\alpha}$ phosphorylation.

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.265.1-265
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• 1997

No Abstract, See Full Text

• Microbiology and Biotechnology Letters
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• v.36 no.4
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• pp.353-359
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• 2008

Hepatitis C virus (HCV) is known as the causative agent of blood transmitted hepatitis. Two viral proteins, E2 and NS5A, are known to exert interferon resistance of HCV via PKR pathway. Here, we report a third protein, the RNA-dependent RNA polymerase (NS5B) of HCV, induced interferon resistance inhibiting p56 pathway. p56 was shown to interact with p48 subunit of eukaryotic initiation factor 3 (eIF3). This interaction inhibited formation of ternary complex in translation initiation. Using dual reporter assay system, we observed that the translation decreased when interferon alpha was added to the culture. But, in the presence of HCV NS5B, the translation partly recovered. NS5B and p48 subunit of eIF3 were shown to interact. This interaction seems to inhibit the interaction between p48 and p56. This is the first report that a virus exerts interferon resistance via p56 pathway.

• Molecules and Cells
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• v.42 no.6
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• pp.495-500
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• 2019

Brain cytoplasmic 200 RNA (BC200 RNA), originally identified as a neuron-specific non-coding RNA, is also observed in various cancer cells that originate from non-neural cells. Studies have revealed diverse functions of BC200 RNA in cancer cells. Accordingly, we hypothesized that BC200 RNA might be modified in cancer cells to generate cancerous BC200 RNA responsible for its cancer-specific functions. Here, we report that BC200 RNA sequences are highly heterogeneous in cancer cells by virtue of multiple adenine nucleotide insertions in the internal A-rich region. The insertion of adenine nucleotides enhances BC200 RNA-mediated translation inhibition, possibly by increasing the binding affinity of BC200 RNA for eIF4A (eukaryotic translation initiation factor 4A).

• International Journal of Internet, Broadcasting and Communication
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• v.13 no.2
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• pp.27-35
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• 2021

NAND flash memory-based SSD needs an internal software, Flash Translation Layer(FTL) to provide traditional block device interface to the host because of its physical constraints, such as erase-before-write and large erase block. However, because useful host-side information cannot be delivered to FTL through the narrow block device interface, SSDs suffer from a variety of problems such as increasing garbage collection overhead, large tail-latency, and unpredictable I/O latency. Otherwise, the new type of SSD, open-channel SSD exposes the internal structure of SSD to the host so that underlying NAND flash memory can be managed directly by the host-level FTL. Especially, I/O data classification by using host-side information can achieve the reduction of garbage collection overhead. In this paper, we propose a new scheme to reduce garbage collection overhead of open-channel SSD by separating the journal from other file data for the journaling filesystem. Because journal has different lifespan with other file data, the Write Amplification Factor (WAF) caused by garbage collection can be reduced. The proposed scheme is implemented by modifying the host-level FTL of Linux and evaluated with both Fio and Filebench. According to the experiment results, the proposed scheme improves I/O performance by 46%~50% while reducing the WAF of open-channel SSDs by more than 33% compared to the previous one.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.15 no.3
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• pp.312-320
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• 2008

Purpose: The purpose of this study was to verify the validity and reliability of the Type D Scale-14 (DS14). Methods: The participants were 288 patients who were diagnosed with angina pectoris, myocardial infarction, and hypertension. DS14 was developed by Denollet(2005) and consists of two domains, 7 items on negative affectivity (NA) and 7 items on social inhibition (SI). The Korean version of DS14 was developed through translation-reversed translation and a preliminary test. Data were collected using a self-report questionnaire. Cronbach $\alpha$ and Guttman split-half were used to test reliability and item analysis and factor analysis for validity. The SPSS program was used. Results: 35.8% of the participants were classified as Type D. Mean score for NA was 16.80 and for SI, 14.10 in Type D participants. For reliability of NA, Cronbach $\alpha$=0.771, and for SI, 0.707. Factor analysis on 12 items(numbers 1 & 3 were excluded as the corrected item-total correlations were below r=0.3) yielded two factors for NA (6 items) and SI (6 items). Number 7 in the NA domain was sorted into the SI domain. Conclusion: The results indicate, the cultural differences were between Europeans and Koreans. Repetition of the research is needed for generalization of DS14.

• Research in Plant Disease
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• v.21 no.1
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• pp.24-26
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• 2015

In April 2014, seedlings of Fallopia multiflora showing wilt symptom were first found at a greenhouse in Punggi-eup, Yeongju-si, Korea. A Fusarium-like fungus was isolated from the wilted plant and it was identified as Fusarium oxysporum based on morphological characteristics and nucleotide sequence data of translation elongation factor 1-${\alpha}$. The fungus isolated from the diseased plant was revealed to be pathogenic to the host plant through pathogenicity tests, and the reisolation of the pathogen confirmed Koch's postulates. This is the first report of Fusarium wilt occurring on Fallopia multiflora in the world.

• The Korean Journal of Mycology
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• v.46 no.3
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• pp.241-248
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• 2018

We isolated endophytic fungi from the coniferous leaves of Abies koreana and Taxus cuspidata inhabiting Mt. Hallasan, Korea. The isolated fungal strains were identified based on a phylogenetic analysis using nucleotide sequences of the internal transcribed spacer region, large subunit region of ribosomal DNA, and translation elongation factor region. Results confirmed four prevously unreported endophytic fungi in Korea: Lachnellula hyalina, Ochrocladosporium elatum, Phacidium lacerum, and Phyllosticta cussoniae. In this report, we describe the morphological characteristics of these fungi and the results of the phylogenetic analysis.

• Korean Journal of Adult Nursing
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• v.21 no.2
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• pp.235-244
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• 2009

Purpose: This research was carried out to evaluate the validity and reliability of the Korean version of the Summary of Diabetes Self-Care Activities Questionnaire (SDSCA) for Korean older adults with type 2 diabetes. Methods: Translation and back-translation were performed to develop the Korean version of the SDSCA. Then the Korean version SDSCA was applied to a sample of 112 older adults who had participated in diabetes self management education in Seoul. The internal consistency and the test-retest reliability were examined to test the reliability. Factor analysis was used to examine the construct validity. Results: The internal consistency measured with Cronbach's alpha was .77 and the total test-retest reliability was .68 with items ranging from .21 to 1.00. As the result of the factor analysis, six factors -foot care, diet, exercise, blood sugar test, medication, and smoking- were revealed as the original instrument subcategories. These six factors explained 81.17% of total variance. Conclusion: The reliability and validity of the Korean version SDSCA Questionnaire was supported for use in older patients with type 2 diabetes in Korea.

• Molecules and Cells
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• v.25 no.4
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• pp.538-544
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• 2008

Activity-dependent local translation in the dendrites of brain neurons plays an important role in the synapse-specific provision of proteins necessary for strengthening synaptic connections. In this study we carried out combined fluorescence in situ hybridization (FISH) and immunocytochemistry (IC) and showed that more than half of the eukaryotic elongation factor 1A (eEF1A) mRNA clusters overlapped with or were immediately adjacent to clusters of PSD-95, a postsynaptic marker, in the dendrites of cultured rat hippocampal neurons. Treatment of the neurons with KCl increased the density of the dendritic eEF1A mRNA clusters more than two-fold. FISH combined with IC revealed that the KCl treatment increased the density of eEF1A mRNA clusters that overlapped with or were immediately adjacent to PSD-95 clusters. These results indicate that KCl treatment increases both the density of eEF1A mRNA clusters and their synaptic association in dendrites of cultured neurons.