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New Records of Endophytic Fungi Isolated from Leaves of Abies koreana and Taxus cuspidata in Korea

구상나무와 주목의 침엽에서 분리된 국내 미기록 내생균

  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Kim, Dong-Yeo (Department of Biology Education, Korea National University of Education) ;
  • Kim, Sae-Rom (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 박혁 (한국교원대학교 생물교육과) ;
  • 김동여 (한국교원대학교 생물교육과) ;
  • 김새롬 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2018.08.14
  • Accepted : 2018.08.25
  • Published : 2018.09.01

Abstract

We isolated endophytic fungi from the coniferous leaves of Abies koreana and Taxus cuspidata inhabiting Mt. Hallasan, Korea. The isolated fungal strains were identified based on a phylogenetic analysis using nucleotide sequences of the internal transcribed spacer region, large subunit region of ribosomal DNA, and translation elongation factor region. Results confirmed four prevously unreported endophytic fungi in Korea: Lachnellula hyalina, Ochrocladosporium elatum, Phacidium lacerum, and Phyllosticta cussoniae. In this report, we describe the morphological characteristics of these fungi and the results of the phylogenetic analysis.

제주도 한라산의 구상나무와 주목의 침엽에서 내생균을 분리하였다. 분리된 균주들은 형태적 특성 및 internal transcribed spacer 영역, large subunitrDNA 영역 및 translation elongation factor 영역 염기서열의 계통분석을 통해 종을 동정하였다. 그 결과 4종의 국내 미기록 내생균을 확인하였고, 확인된 종은 각각 Lachnellula hyalina, Ochrocladosporium elatum, Phacidium lacerum, Phyllosticta cussoniae이다. 확인된 4종의 미기록 내생균 균주의 형태적 특성 및 염기서열 계통분석의 결과에 대해 기술하였다.

Keywords

References

  1. Carroll G. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9. https://doi.org/10.2307/1943154
  2. Schulz B, Boyle C, Draeger S, Rommert AK, Krohn K. Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol Res 2002;106:996-1004. https://doi.org/10.1017/S0953756202006342
  3. Christensen MJ. Antifungal activity in grasses infected with Acremonium and Epichloe endophytes. Australas Plant Pathol 1996;25:186-91. https://doi.org/10.1071/AP96032
  4. Strobel GA, Hess WM, Ford E, Sidhu RS, Yang X. Taxol from fungal endophytes and the issue of biodiversity. J Ind Microbiol Biotechnol 1996;17:417-23. https://doi.org/10.1007/BF01574772
  5. Findlay JA, Buthelezi S, Lavoie R, Pena-Rodriguez L, Miller JD. Bioactive isocoumarins and related metabolites from conifer endophytes. J Nat Prod 1995;58:1759-66. https://doi.org/10.1021/np50125a021
  6. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
  7. Moncalvo JM, Lutzoni FM, Rehner SA, Johnson J, Vilgalys R. Phylogenetic relationships of agaric fungi based on nuclear large subunit ribosomal DNA sequences. Syst Biol 2000;49:278-305. https://doi.org/10.1093/sysbio/49.2.278
  8. Rehner SA, Buckley E. A Beauveria phylogeny inferred from nuclear ITS and EF1-${\alpha}$ sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 2005;97:84-98.
  9. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
  10. Dharne CG. Taxonomic investigations on the discomycetous genus Lachnellula Karst. J Phytopathol 1965;53:101-44. https://doi.org/10.1111/j.1439-0434.1965.tb02194.x
  11. Crous PW, Braun U, Schubert K, Groenewald JZ. Delimiting Cladosporium from morphologically similar genera. Stud Mycol 2007;58:33-56. https://doi.org/10.3114/sim.2007.58.02
  12. Crous PW, Quaedvlieg W, Hansen K, Hawksworth DL, Groenewald JZ. Phacidium and Ceuthospora (Phacidiaceae) are congeneric: taxonomic and nomenclatural implications. IMA Fungus 2014;5:173-93. https://doi.org/10.5598/imafungus.2014.05.02.02
  13. Fries E. Observationes mycologicae praecipue ad illustrandam Floram Suecicam. Copenhagen: Gerhardi Bonnier; 1818.
  14. Cejp K. Some members of the Sphaeropsidales from South Africa. Bothalia 1971;10:341-5.
  15. Wikee S, Lombard L, Nakashima C, Motohashi K, Chukeatirote E, Cheewangkoon R, McKenzie EH, Hyde KD, Crous PW. A phylogenetic re-evaluation of Phyllosticta (Botryosphaeriales). Stud Mycol 2013;76:1-29. https://doi.org/10.3114/sim0019