• Journal of the Korean Society for Nondestructive Testing
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• v.28 no.5
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• pp.407-415
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• 2008

The tubes in heat exchanger are typically made from copper alloy, stainless steel, carbon steel, titanium alloy material. type-439 ferritic stainless steel is ferromagnetic material, and furnish higher heat transfer rates than austenitic stainless steels and higher resistance to corrosion-induced flaws. Ferritic stainless steel can typically be found in low-pressure(LP) feedwater heaters and moisture separator reheaters(MSRs). LP feedwater heaters generally utilize thin wall type-439 stainless steel tubing, whereas MSRs typically employ a heavier wall tubing with integral fins. Service-induced damage can occur on the OD(outside diameter) surface of type-439 ferritic stainless steel tubing which is employed for MSRs tubing, and the most typical damage mechanism is vibration-induced tube-to-TSP(tube support plate) wear and fatigue cracking. The wear has been reported that occurs mainly on the OD surface. Accordingly, in this study, we have evaluated the flaw sizing capability of magnetic saturation eddy current technique using magnetic saturation probe and flawed specimen.

• Journal of Korean Society of Steel Construction
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• v.29 no.6
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• pp.411-422
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• 2017

In this study, to investigate the seismic performance of beam-column joints using concrete-encased and -filled circular steel tube(CEFT) columns, two types of tests were performed: (1) column - flange tension test and (2) beam - column joint cyclic load test. In column - flange tension test, test parameters were concrete encasement and connection details: flange width and strengthening rebar. Five specimens were tested to investigate the load-carrying capacity and the failure mode. Test results showed that increase of flange width from 200mm to 350mm result in increase of connection strength and stiffness by 61% and 56%, respectively. Structural performances were further improved with addition of tensile rebars by 35% and 92%, respectively. In cyclic loading test, three exterior beam-column joints were prepared. Test parameters were strengthening details including additional tensile rebars, thickened steel tube, and vertical plate connection. In all joint specimens, flexural yielding of beam was occurred with limited damages in the connection regions. In particular, flexural capacity of beam-column joint was increased due to additional load transfer through tube - beam web connection. Also, connection details such as increase of tube thickness and using vertical plate connection were effective in improving the resistance of panel zone.

• Korean Journal of Soil Science and Fertilizer
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• v.25 no.4
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• pp.387-393
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• 1992

The fate of inoculum strain of Bradyrhizobium japonicum was studied by using genetically marked strain. RJB6 $str^rnal^rneo^r$. A spontaneous mutant of B. japonicum isolated from nodules was made to have antibiotic resistance against streptomycin and nalidixic acid. In order to make genetically marked strain, neomycine resistant gene(Tn5) was introduced into this spontaneous mutant by conjugation with E. coli containing pSUP2021. The southern hybridization was carried out to confirm the plasmid insertion. Hybridization of chromosome DNA using pSUP2021(Tn5) as a probe showed that Tn5 was located on the 4.9kb fragment of chromosome. Soybean seeds were planted into a soil previously cultivated with soybean and inoculated with different cell densities of marked strain. Fourty days after planting, the inoculation effects on nodule number, nodule fresh weight, plant height and nitrogen content in the plot inoculated with heavy cell suspension was a little better than those in the plot with low inoculation. The recovery percentage of the marked strains was about 12% in the plot inoculated with heavy density cell suspension, while 5% in the plot inoculated with low cell suspension.

• Journal of Korean Tunnelling and Underground Space Association
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• v.15 no.3
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• pp.289-300
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• 2013

This study numerically considered the characteristic of smoke movement and the effect of hot smoke gas on tunnel wall surface temperature during road tunnel fire under boundary condition of fire growth curve that is applied to fire analysis in road tunnels. The maximum heat release rate were 20 MW and 100 MW and tunnel air velocities were 2.5 m/s and velocity induced by thermal buoyancy respectively, also the cooling effect of tunnel wall was considered. As results, when tunnel air velocity was constant at 2.5 m/s during tunnel fire, due to the cooling effect of tunnel wall, the smoke layer was rapidly descent after some distance and it flowed the same patterns at the downstream. When heat release rate was 100 MW (and jet fan was not installed), the maximum temperature of tunnel wall surface has risen up to $615^{\circ}C$. The heat transfer coefficient of tunnel wall surface was varied from 13 to $23W/m^2^{\circ}C$ approximately.

• Reproductive and Developmental Biology
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• v.33 no.2
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• pp.107-111
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• 2009

The transfection efficiency of a transgene into pig and goat fetal fibroblast cells (PFF and GFF, respectively) was tested using co-transfection of a human tissue-type plasminogen activator (tPA) transgene and neomycin-resistant ($Neo^r$) gene, followed by G418 selection. To initially test G418 resistance, GFF and PFF were incubated in culture medium containing different concentration of G418 for 2 weeks, and cell survival was monitored over time. Based on the obtained results, the concentrations chosen for G418 selection were 800 ug/ml and 200 ug/ml for GFF and PFF, respectively. For co-transfection experiments, the pBC1/tPA and $Neo^r$ vectors were co-transfected into GFF and PFF ($1{\times}10^6$ cells in each case) using the FuGENE6 transfection reagent, and resistant colonies were obtained following 14 days of G418 selection. We obtained 96 and 93 drug-resistant colonies of GFF and PFF, respectively, only 54 and 39 of which, respectively, continued proliferating after drug selection. PCR-based screening revealed that 23 out of 54 analyzed GFF colonies and 5 out of 39 analyzed PFF colonies contained insertion of the tPA gene. Thus, the experimentally determined transfection efficiencies for tPA gene co-transfection with the $Neo^r$ gene were 42.6% for GFF and 12.8% for PFF. These findings suggest that co-transfection of a transgene with the $Neo^r$ gene can aid in the successful integration of the transgene into fetal fibroblast cells.

• Fisheries and Aquatic Sciences
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• v.22 no.12
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• pp.30.1-30.11
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• 2019

Marine natural products isolated from the sponge Fascaplysinopsis cf reticulata, in French Polynesia, were investigated as an alternative to antibiotics to control pathogens in aquaculture. The overuse of antibiotics in aquaculture is largely considered to be an environmental pollution, because it supports the transfer of antibiotic resistance genes within the aquatic environment. One environmentally friendly alternative to antibiotics is the use of quorum sensing inhibitors (QSIs). Quorum sensing (QS) is a regulatory mechanism in bacteria which control virulence factors through the secretion of autoinducers (AIs), such as acyl-homoserine lactone (AHL) in gram-negative bacteria. Vibrio harveyi QS is controlled through three parallel pathways: HAI-1, AI-2, and CAI-1. Bioassay-guided purification of F. cf reticulata extract was conducted on two bacterial species, i.e., Tenacibaculum maritimum and V. harveyi for antibiotic and QS inhibition bioactivities. Toxicity bioassay of fractions was also evaluated on the freshwater fish Poecilia reticulata and the marine fish Acanthurus triostegus. Cyclohexanic and dichloromethane fractions of F. cf reticulata exhibited QS inhibition on V. harveyi and antibiotic bioactivities on V. harveyi and T. maritimum, respectively. Palauolide (1) and fascaplysin (2) were purified as major molecules from the cyclohexanic and dichloromethane fractions, respectively. Palauolide inhibited QS of V. harveyi through HAI-1 QS pathway at 50 ㎍ ml^-1 (26 μM), while fascaplysin affected the bacterial growth of V. harveyi (50 ㎍ ml^-1) and T. maritimum (0.25 ㎍). The toxicity of fascaplysin-enriched fraction (FEF) was evaluated and exhibited a toxic effect against fish at 50 ㎍ ml^-1. This study demonstrated for the first time the QSI potential of palauolide (1). Future research may assess the toxicity of both the cyclohexanic fraction of the sponge and palauolide (1) on fish, to confirm their potential as alternative to antibiotics in fish farming.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.3
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• pp.209-214
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• 2017

Most of the present potential transformers of train vehicles are of the oil-type filled with insulating oil and are susceptible to problems such as explosion due to the increase in the internal pressure during train operation and poor reliability near the end of their life cycle. As a solution to this problem, it is necessary to develop a molded dry-type potential transformer with excellent pressure-resistance performance using insulating resin. In order to localize the product, the Korea Railroad Research Institute has been developing a molded dry-type potential transformer. As part of this research, it is necessary to analyze the vibration characteristics of the developed product and to check the transformer performance in a vibration environment. In this study, a resonance test and simulated long-term life test of the developed product were conducted according to the KS R 9144 and IEC 61373 standards, respectively, which are vibration test methods for railway vehicle parts. Their natural frequencies were analyzed by comparing the results of the numerical modal analysis and resonance test, in order to confirm their adherence to the standards. Also, the performance test after the simulated long-term life test confirmed that the operation of the developed transformer was not problematic even in a long-time vibration environment.

• Journal of the Korean Chemical Society
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• v.30 no.3
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• pp.273-281
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• 1986

This study is focused on the correct measurement of the equations for the determination of the impedance parameters-the differential capacity of the double layer $C_d$, solution resistance $R_Q$, transfer resitance $R_i$, and adsorption pseudcapacity $C_{\phi}$/ The application of only an imaginary part of complex function of ${\omega}$ at the sinusoidal steady state indicates the following equations of total impedance: at low frequency $|Z_{LF}|=1/{\omega}_1\;C_{\phi}\;{\sqrt{1+{{\omega}_1}^2/{\omega}^2}$, at high frequency $|Z_{HF}|={\omega}_2/({\omega}_1{\omega}_3C{\phi})({\omega}^2+{{\omega}_2}^2)\;{\sqrt{{({\omega}^2+{\omega}_2{\omega}_3)}^2+{({\omega}_2{\omega}-{\omega_3{\omega})^2}}$. The values of the total impedance of cell, phase angle, and cell current that are necessary for the calculations of impedance parameters were experimentally measured from 200 to 6000Hz for the following supporting electrolytes, 0.5M $Na_2SO_4$, 1M NaCl, 19.373% sea water, 1M HCl, 1M $KNO_3$ and for $10^{-2}M$ KI and 60mM DBNA (Di-iso-Butylnitrosoamine) in these supporting electrolytes. The derived equations in this study shows that the values of impedance parameters of $C_d,\;C_{\phi},\;R_i\;and\;R_Q\;are\;15{\sim}40\;{\mu}F/cm^2,\;162{\sim}758\;{\mu}F/cm^2\;11.5{\sim}57.6\;ohm{\cdot}cm^2\;and\;0.5{\times}10^{-2}{\sim}4.1{\times}10^{-2}\;ohm{\cdot}cm^2$ respectively.

• Journal of Korea Port Economic Association
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• v.29 no.1
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• pp.99-122
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• 2013

International Exposition Yeosu Korea 2012 was held from May 12th 2012 to August 12th 2012 in New Port area of Yeosu-city, Jeollanam-do, Korea. 105 Nations and 10 international organizations participated in this mega event, and the total number of visitors amounts to 8.2 million. This study appraises the effectiveness of preparation for Yeosu Exposition. The main focus of this study is not on criticizing the problems that occurred in the transport measures. Instead, it looks at the current aspects of establishment and execution process and suggests how they can be applied differently in future transport management for international mega events held in Korea. The suggestions of this study are as follows. First, when establishing transport management plans for international mega events, the geographical features and trip resistance of the venue must be taken into consideration. Second, tasks should be distributed distinctly between the central government and the local government in order to prevent any dissension or inefficiency. Third, transport management needs to be consistent and transport guidance should be efficient in an effort to minimize visitor inconvenience. Last, transport management must be regarded as objective and rational from the outside view and the budget should follow timely based on this account.

• Korean Chemical Engineering Research
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• v.49 no.2
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• pp.129-136
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• 2011

Simulated Moving Bed(SMB) process consists of multiple chromatographic columns, which are usually partitioned into four zones. Such a process characteristic allows a continuous binary separations those are impracticable in conventional batch chromatographic processes. Compared with batch chromatography, SMB has advantages of continuity, high purity and productivity. Various researches have been reported for the integration of reaction and recovery during process operation on the purpose of economics and effectiveness. Simulated Moving Bed Reactor(SMBR) is introduced to combine SMB as a continuous separation process and reactor. Several cases of SMBR have been reported for diverse reactions with catalytic, enzymatic and chemical reaction on ion exchange resin as main streams. With an early type of fixed bed using catalyst, SMBR has been developed as SMB using fluidized enzyme, SMB with immobilized enzyme and SMB with discrete reaction region. For simple modeling and optimization of SMBR, a method considering convection only is possible. A complex method considering axial dispersion and mass transfer resistance is needed to explain the real behavior of solutes in SMBR. By combining reaction and separation, SMBR has benefits of lower installation cost by minimizing equipment use, higher purity and yield by avoiding the equilibrium restriction in case of reversible reaction.