• Journal of fish pathology
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• v.33 no.2
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• pp.103-109
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• 2020

In order to use nervous necrosis virus (NNV) virus-like particles (VLPs) as a delivery tool for heterologous antigens or plasmids, we attempted to produce red-spotted grouper nervous necrosis virus (RGNNV) VLPs displaying a partial region of viral hemorrhagic septicemia virus (VHSV) glycoprotein at the surface and VLPs that are harboring DNA vaccine plasmids within the VLP. A peptide encoding 105 amino acids of VHSV glycoprotein was genetically inserted in the loop region of NNV capsid gene, and VLPs expressing the partial part of VHSV glycoprotein were successfully produced. However, in the transmission electron microscope analysis, the shape and size of the partial VHSV glycoprotein-expressing NNV VLPs were irregular and variable, respectively, indicating that the normal assembly of capsid proteins was inhibited by the relatively long foreign peptide (105 aa) on the loop region. To encapsulate by simultaneous transformation with both NNV capsid gene expressing plasmids and DNA vaccine plasmids (having an eGFP expressing cassette under the CMV promoter), NNV VLPs containing plasmids were produced. The encapsulation of plasmids in the NNV VLPs was demonstrated by PCR and cells exposed to the VLPs encapsulating DNA vaccine plasmids showed fluorescence. These results suggest that the encapsulation of plasmids in NNV VLPs can be done with a simple one-step process, excluding the process of disassembly-reassembly of VLPs, and NNV VLPs can be used as a delivery tool for DNA vaccine vectors.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.287-287
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• 2023

Surface water-groundwater interaction (SWGI) is an important hydrological process that influences both the quantity and quality of water resources. However, regional scale SWGI model calibration and uncertainty analysis have been a challenge because integrated models inherently carry a vast number of parameters, modeling assumptions, and inputs, potentially leaving little time and budget to explore questions related to model performance and forecasting. In this study, we have proposed the application of iterative ensemble smoother (IES) for uncertainty analysis and calibration of the widely used integrated surface-subsurface model, SWAT-MODFLOW. SWAT-MODFLOW integrates Soil and Water Assessment Tool (SWAT) and a three-dimensional finite difference model (MODFLOW). The model was calibrated using a parameter estimation tool (PEST). The major advantage of the employed IES is that the number of model runs required for the calibration of an ensemble is independent of the number of adjustable parameters. The pilot point approach was followed to calibrate the aquifer parameters, namely hydraulic conductivity, specific storage, and specific yield. The parameter estimation process for the SWAT model focused primarily on surface-related parameters. The uncertainties both in the streamflow and groundwater level were assessed. The work presented provides valuable insights for future endeavors in coupled surface-subsurface modeling, data collection, model development, and informed decision-making.

• Journal of Microbiology and Biotechnology
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• v.33 no.11
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• pp.1457-1466
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• 2023

Enteric fever is caused by typhoidal Salmonella serovars (Typhi, Paratyphi A, Paratyphi B, and Paratyphi C). Owing to the importance of Salmonella serovars in clinics and public hygiene, reliable diagnostics for typhoidal serovars are crucial. This study aimed to develop a novel diagnostic tool for typhoidal Salmonella serovars and evaluate the use of human blood for clinically diagnosing enteric fever. Five genes were selected to produce specific PCR results against typhoidal Salmonella serovars based on the genes of Salmonella Typhi. Heptaplex PCR, including genetic markers of generic Salmonella, Salmonella enterica subsp. enterica, and typhoidal Salmonella serovars, was developed. Typhoidal Salmonella heptaplex PCR using genomic DNAs from 200 Salmonella strains (112 serovars) provided specifically amplified PCR products for each typhoidal Salmonella serovar. These results suggest that heptaplex PCR can sufficiently discriminate between typhoidal and non-typhoidal Salmonella serovars. Heptaplex PCR was applied to Salmonella-spiked blood cultures directly and provided diagnostic results after 12- or 13.5-h blood culture. Additionally, it demonstrated diagnostic performance with colonies recovered from a 6-h blood culture. This study provides a reliable DNA-based tool for diagnosing typhoidal Salmonella serovars that may be useful in clinical microbiology and epidemiology.

• Journal of the Korean Professional Engineers Association
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• v.21 no.1
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• pp.5-12
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• 1988

The purpose of this paper is to investigate on the optimum coating thickness layer of TiC-Al$_2$O$_3$ coated cemented carbide tool. Chemical Vapor Deposition (CVD) of a thick film of TiC-A1$_2$O$_3$ on a cemented carbide produces an intermediate layer, $1.5mutextrm{m}$, 4.5${\mu}{\textrm}{m}$, 7.5${\mu}{\textrm}{m}$ 10.5${\mu}{\textrm}{m}$, 4 kind of TiC between the substrate and the $1.5mutextrm{m}$ constant thick A1$_2$O$_3$ coating. Experiments were carried out with the test relationship between coating thickness and shear angle, surface roughness, cutting force, microphotograph of crater wear, flank wear, tool life. From the experimental results, it was found that the optimum coating thickness of TiC-A1$_2$O$_3$ is 6${\mu}{\textrm}{m}$. Although the coating thickness layer 9${\mu}{\textrm}{m}$. 12${\mu}{\textrm}{m}$ have a much loger tool wear than an 3${\mu}{\textrm}{m}$, 6${\mu}{\textrm}{m}$ coating tool in cutting condition feed 0.05mm/rev, and the condition of feed 0.2mm/rev, 0.3mm/rev has upon in the shot time phenomenon of chipping.

• The Korean Journal of Applied Statistics
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• v.18 no.2
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• pp.487-497
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• 2005

The instruction assessment, which can be a tool to realize a class of high quality, evaluates instruction system and process. The result data of the instruction assessment is analysed and used to reorganize lecture contents or to improve teaching method. It enhances qualitative level of university education. The goal of the instruction assessment is to be a tool that provides high qualitative education to raise students' satisfaction. It is important to improve education service quality through instruction assessment as a useful tool for measuring service quality. The suggestion of valid tool for improving instruction service quality is described in this paper.

• Journal of the Korean Society for Precision Engineering
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• v.22 no.6 s.171
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• pp.222-231
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• 2005

Recently, life cycle and lead-time of products have been shortened with the demand of customers. Therefore, it is important to reduce time and cost at the stage of manufacturing trial molds. In order to realize a three-dimensional shape on CAD, the machining process has been widely used because it offers practical advantages such as precision and versatility. However, the traditional machining process needs a large amount of time in cutting a product and the remained material causes trouble such as inconvenience for clarity. In this work, a new rapid manufacturing process using the hot tool, Rapid Heat Ablation process, has been developed to overcome such limitations. While the hot tool moves the predetermined path, the heat of the tool decomposes the remained material. The radius of heat affect ed zone related to process parameters was investigated through experiments to improve the quality of ablated parts. In order to examine the applicability of the proposed process, three-dimensional shapes such as hemisphere and standard test part, wereablatedutilizingtheapparatus.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2001.04a
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• pp.40-45
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• 2001

The relationship between structural geometry and number of life cycles to failure is investigated to improve the fatigue life of structural components. The linear elastic fracture mechanics(LEFM) approach is integrated with shape optimal design methodology. The primary objective of this study is to decide an optimal shape for enhancing the life of the structure. The results from LEFM analyses are used in the fatigue model to predict the life of the structure before failure is occurred. The shape of the structure is optimized by using the growth strain method. Relevant issues such as problem formulation, finite element modeling are explained. Three design examples are solved, and the results show that, with proper shape changes, the life of structural systems subjected to fatigue loads can be enhanced significantly.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 1998.03a
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• pp.269-273
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• 1998

The failure of material structures or mechanical system is considered as a direct or indirect result of fatigue. In the design of mechanical structure for estimating of reliability, the prediction of failure life is the most important failure mode to be considered. However, because of a complicated behavior of fatigue in mechanical structure, the analysis of fatigue is in need of much researches on life prediction. This document presents a prediction of fatigue life of the SAPH45 steel, which is extensively for vehicle frame. The method using lethargy coefficient and stress distribution factor at pediction of fatigue life based on the consideration of the failure characteristics from the tensile test should be provided in this study.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.17 no.1
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• pp.137-143
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• 2008

Thermal fatigue life prediction for solder joints becomes the most critical issue in present microelectronic packaging industry. And lead-free solder is quickly becoming a reality in electronic manufacturing fields. This trend requires life prediction models for new solder alloy systems. This paper describes the life prediction models for SnAgCu and SnPb solder joints, based upon non-linear finite element analysis (FEA). In case of analyses of the SnAgCu solder joints, two kinds of shapes are used. As a result, it is found that the SnAgCu solder has longer fatigue life than the SnPb solder in temperature cycling analyses.

• Communications for Statistical Applications and Methods
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• v.29 no.5
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• pp.513-532
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• 2022

In this paper, the use of Bayes factors and the deviance information criterion for model selection are compared in a Bayesian accelerated life testing setup. In Bayesian accelerated life testing, the most used tool for model comparison is the deviance information criterion. An alternative and more formal approach is to use Bayes factors to compare models. However, Bayesian accelerated life testing models with more than one stressor often have mathematically intractable posterior distributions and Markov chain Monte Carlo methods are employed to obtain posterior samples to base inference on. The computation of the marginal likelihood is challenging when working with such complex models. In this paper, methods for approximating the marginal likelihood and the application thereof in the accelerated life testing paradigm are explored for dual-stress models. A simulation study is also included, where Bayes factors using the different approximation methods and the deviance information are compared.