• 제목/요약/키워드: Tissue Engineering

검색결과 1,867건 처리시간 0.547초

Effects of Testosterone on Adipose Tissue Metabolism (지방조직대사에 대한 testosterone의 영향)

  • Jeong, Sunhyo
    • Journal of the Korea Institute of Information and Communication Engineering
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    • 제17권12호
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    • pp.2995-3000
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    • 2013

  • We investigated the effects of testosterone on the improvement of white adipose tissue explant and its molecular mechanism in adipose tissue of high fat diet-fed male castrated (CAST) mice. The CAST mice treated with testosterone had lower adipose tissue weights, the average size of adipocytes and mRNA levels of $C/EBP{\alpha}$ as well as adipocyte marker genes than the vehicle-treated CAST mice. These results suggest that testosterone prevent the expression of $C/EBP{\alpha}$ and $C/EBP{\alpha}$-mediated adipocyte marker genes, resulting in decreased adipose tissue mass and adipocyte metabolism in male CAST mice. Moreover, this study give a valuable molecular and biological knowledge on testosterone therapy in obese hypogonadal men.

  • https://doi.org/10.6109/jkiice.2013.17.12.2995 인용 PDF KSCI

Local Silencing of Connective Tissue Growth Factor by siRNA/Peptide Improves Dermal Collagen Arrangements

  • Cho Lee, Ae-Ri;Woo, Inhae
    • Tissue Engineering and Regenerative Medicine
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    • 제15권6호
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    • pp.711-719
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    • 2018

  • BACKGROUND: Collagen organization within tissues has a critical role in wound regeneration. Collagen fibril diameter, arrangements and maturity between connective tissue growth factor (CTGF) small interfering RNA (siRNA) and mismatch scrambled siRNA-treated wound were compared to evaluate the efficacy of CTGF siRNA as a future implement for scar preventive medicine. METHODS: Nanocomplexes of CTGF small interfering RNA (CTGF siRNA) with cell penetrating peptides (KALA and $MPG^{{\Delta}NLS}$) were formulated and their effects on CTGF downregulation, collagen fibril diameter and arrangement were investigated. Various ratios of CTGF siRNA and peptide complexes were prepared and down-regulation were evaluated by immunoblot analysis. Control and CTGF siRNA modified cells-populated collagen lattices were prepared and rates of contraction measured. Collagen organization in rabbit ear 8 mm biopsy punch wound at 1 day to 8 wks post injury time were investigated by transmission electron microscopy and histology was investigated with Olympus System and TS-Auto software. CONCLUSION: CTGF expression was down-regulated to 40% of control by CTGF siRNA/KALA (1:24) complexes (p<0.01) and collagen lattice contraction was inhibited. However, down-regulated of CTGF by CTGF $siRNA/MPG^{{\Delta}NLS}$ complexes was not statistically significant. CTGF KALA-treated wound appeared with well formed-basket weave pattern of collagen fibrils with mean diameter of $128{\pm}22nm$ (n = 821). Mismatch siRNA/KALA-treated wound showed a high frequency of parallel small diameter fibrils (mean $90{\pm}20nm$, n = 563). CONCLUSION: Controlling over-expression of CTGF by peptide-mediated siRNA delivery could improve the collagen orientation and tissue remodeling in full thickness rabbit ear wound.

  • https://doi.org/10.1007/s13770-018-0166-2 인용

Fabrication of Nanfiber-based Medical scaffolds and their Prospective Application (나노파이버 기반의 의료용 지지체 제작 기술 및 응용)

  • 신호준;이창훈;조인희;김인애;이용재;박기동;신정욱
    • Proceedings of the Korean Society of Machine Tool Engineers Conference
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    • 한국공작기계학회 2004년도 춘계학술대회 논문집
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    • pp.518-523
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    • 2004

  • PLGA was suggested. Under various conditions, their diameters and porosity as well as mechanical strength were evaluated. In addition to those, cell(chondrocyte) proliferation and formation of extracelluar matrices were also investigated along with the conventional membrane type PLGA scaffolds for the potential use in tissue engineering. As conclusions, this type of scaffold showed a potential of application to tissue engineering in view of mechanical stability as well as cellular responses.

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Development of a Multipurpose Cure-appliance Using Microwave (마이크로파를 이용한 다목적 치료기의 개발)

  • 박병욱;정병선
    • Journal of Biomedical Engineering Research
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    • 제10권2호
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    • pp.179-184
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    • 1989

  • There are microwave hyperthermia, microwave surgical knife, and microwave tissue coagulator etc. as the cure-appliance using microwave. The object of this paper is to develope the multipurpose cure-appliance which has the functions that can be used intestinal tumor as well as the existing superficial tumor. And this also have the function of surgical knife and tissue coagulator with endoscope. And then ascertain its functions through the experiments, and to suggest the direction for reserch hereafter.

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