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Effects of Artificial Shading on Flowering and Growth of Maesa japonica Seedlings (차광 처리가 빌레나무(Maesa japonica)의 개화 및 생장에 미치는 영향)

  • Park, Min Ji;Seo, Yeon Ok;Choi, Hyung Soon;Choi, Byoung Ki;Im, Eun Young;Yang, Ju Eun;Lee, Chae bin
    • Korean Journal of Plant Resources
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    • v.34 no.5
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    • pp.462-469
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    • 2021
  • Maesa japonica (Thunb.) Moritzi & Zoll. is a rare evergreen shrub that occurs in west Gotjawal, Jeju island, Korea. This study was conducted to investigate effects of an artificial shading on flowering and growth characteristics of M. japonica seedlings. The level of shading had been set to be 35%, 55% and 75% using shading net. The stage of flowering and flowering ratio, seedling height, the number of leaves and stems, leaf area were measured. Flowering timings were delayed by shading. The number of flowering seedlings per plot and inflorescences per seedling were the most in 55% shading level. Tendency of decreased seedling height with increased shading level was shown. The number of leaves and stems were the fewest values in 75% shading level. The increased biomass with decreased shading level was statistically significant. Aboveground and underground biomass were 2.1 and 1.7 times higher in shading 35% than in shading 75%, respectively. Meanwhile, all seedlings in non-shading plots were dead in winter. Such might be speculated as results of the light stress. As a result, we conclude that M. japonica vitally demands the shading for growth and the optimal shading levels for growth and flowering are 35% and 55%, respectively.

Diagnosis of the growth status and actual condition of the remaining old trees in the village - Focused on Hyeongok-myeon in Gyeongju-si - (마을 내 잔존 노거수의 생육현황 및 실태진단 - 경주시 현곡면을 중심으로 -)

  • Kim, Young-Hun;Deng, Bei-Jia;Chen, Geng;You, Ju-Han
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.23 no.6
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    • pp.109-123
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    • 2020
  • The purpose of this study is to provide basic data for the establishment of future countermeasures by diagnosing the growth status and actual condition of undesignated old trees distributed in Hyeongok-myeon, Gyeongju, Gyeongsangbuk-do. The number of old trees surveyed was 2 weeks in Gajeong-ri, 2 weeks in Haguri, 3 weeks in Nae-Tae-ri, 1 week in Nawon-ri, 6 weeks in Oryu-ri, 3 weeks in Sangguri, and 2 weeks in Sohyeon-ri, The trees species composition was 6 trees Celtis sinensis Pers., 1 Diospyros lotus L. trees, 4 trees Salix chaenomeloides Kimura trees, 2 Styphnolobium japonicum L. trees, and 7 Zelkova serrata (Thunb.) Makino trees. Growth status is 7.1~22.0m in height, 14.6~25.1m in long axis, 10.2~19.2m in short axis, root diameter is 76.0~236.4cm, diamter at breast height is 67.0~220.0cm, soil acidity is pH4.9~7.0, soil The hardness was measured to be 4.0-27.0mm. The result grade of the scoring data of health information is represented by monitoring generally, monitoring critically, and absolute monitoring, and it was confirmed that out of the 20 trees population in Hyeongok-myeon, the general monitoring grade was 7 weeks, the major monitoring grade was 13 weeks, and there was no absolute monitoring grade. Accordingly, the number of old trees of the general surveillance level was maintained at the current level, and ecological surgical operations were introduced for the major surveillance level, but the case of village forests should be different, and sequential treatments were the old tree urgently needed. The level and bark of the target tree, the state of the crown, the root exposure, the presence of pests and pests, the vitality and the ground condition, the degree of pollution are normal, poor, or very poor, operation and protection management, soil improvement, removal of cover, and disinfection were urgently needed for the old trees with the surveyed data. In order to compensate for these matters, continuous monitoring and management measures for the old number should be sought.

Genotoxicity Study of Immature Green Persimmon Extract (풋감 주정 추출물의 유전독성 연구)

  • Ham, Young-Min;Yoon, Seon-A;Hyun, Ho Bong;Go, Boram;Jung, Yong-Hwan;Oh, Dae-Ju;Yoon, Weon-Jong
    • Journal of Food Hygiene and Safety
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    • v.35 no.6
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    • pp.567-573
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    • 2020
  • The persimmon is commonly cultivated in temperate regions of the world, including China, Korea, Japan, Brazil, Turkey, and Italy. In some Asian cultures, consumers are aware of the health claims related to the persimmon and its functional ingredients. The rich phytochemistry of the persimmon has opened new avenues of research on diet-based regimens designed to cure various ailments. This study was conducted to identify the genotoxicity of immature green persimmon (Diospyros kaki THUNB.) extract (DKA). The bacterial reverse mutation assay, the chromosomal aberration assay, and the mammalian micronucleus test were performed to determine the DKA genotoxicity. The result of the bacterial reverse mutation assay revealed that the DKA did not induce mutagenicity in Salmonella typhimurium TA98, TA100, TA1535, TA1537 and Escherichia coli WP2uvrA with or without metabolic activation of S9 mixture. The oral administration of DKA also caused no significant increase in the number of micronucleated polychromatic erythrocytes or in the mean ratio of polychromatic to total erythrocytes. In addition, DKA did not cause a significant chromosome aberration on CHL cells in the presence or absence of S9 activation. In conclusion, DKA could be considered as a reliable and safe functional food since no toxicity was found under the condition of this study.

A Study on 𝛽-glucan, Ginsenoside Content, 2,2-diphenyl-1-picrylhydrazyl Free Radical Scavenging Activity, Anti-inflammatory Activity and Safety of Herbal Medicine Mix - Iksooyoungjingogami with Scutellariae Radix and Houttuynia cordata Thunb (황금, 어성초를 배합한 익수영진고가미 한약재배합물의 베타글루칸, 진세노사이드 함량, 2,2-diphenyl-1-picrylhydrazyl Free Radical 소거 활성, 항염 활성 및 안전성 연구)

  • Kim, Myeong-Hun;Moon, Yang-Seon;Kang, Sang-Mi;Kim, Heyong-Seok;Kim, Seon-Jong;Na, Chang-Su
    • Journal of Korean Medicine Rehabilitation
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    • v.32 no.2
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    • pp.1-17
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    • 2022
  • Objectives This study was conducted to investigate the beta-glucan & ginsenoside content, antioxidant activity, anti-inflammatory effect and safety of herbal medicine mix. Methods The marker compounds contents, antioxidant activity and safety of herbal medicine mix were tested. The contents of beta-glucan and ginsenoside Rg3 were measured, the antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, anti-inflammatory and a safety test was conducted via single dose toxicity assessment. Results Analyzing the contents of marker compounds showed 362.3 mg/g of beta-glucan, and 0.4184 mg/g of ginsenoside Rg3. In the DPPH free radical scavenging activity, the IC50 of herbal medicine mix, was 0.146%. The scavenging activity of herbal medicine mix was 88.28% activity at 0.5% concentration, and 90.61% activity at 5% concentration. In the lipopolysaccharides (LPS) anti-inflammatory test, the herbal remix showed a significant decrease in tumor necrosis factor-alpha (TNF-𝛼) and interleukin-6 (IL-6) compared to the LPS-induced group. In the single dose toxicity test of herbal medicine mix, a dose of 2,000 mg/kg body weight (BW) was set at its highest capacity and observed after oral administration to female and male rats. No toxicological findings were recognized. It was observed that the resulting lethal dose can be set to 2,000 mg/kg BW or higher for both females and males. Conclusions The results of the experiment on herbal medicine mix showed that the marker compounds contents were beta-glucan and ginsenoside Rg3, that antioxidant activity was observed through the DPPH free radical scavenging activity, anti-inflammatory effect was observed through TNF-𝛼 and IL-6 measurement, and safety was confirmed through the single dose toxicity assessment.

Characteristics of the Species Composition by Plant Community in the Shincheon Wetland of Mangyeong River, Jeonbuk (만경강 신천습지의 식물군락별 종조성적 특성)

  • Kwang-Jin, Cho;Jung-A, Lee;Jeoncheol, Lim;Yeounsu, Chu
    • Journal of Environmental Impact Assessment
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    • v.31 no.6
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    • pp.409-422
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    • 2022
  • Riverine wetlands are an important element of the river ecosystem and account for approximately 38% of the inland wetlands surveyed so far. The Shincheon Wetland located in Mangyeong River is also a channel wetland as the flow rate is slowed by the constructed weirs, leading to sediment accumulation. To identify the conservation value and ecological characteristics of Shincheon Wetland, its vegetation and plant diversity were identified using a phytosociological method, and a total of 45 vegetation-related datasets were collected. Overall, 24 plant communities, comprising a total of 153 taxa (49 families, 117 genera, 146 species, 2 subspecies, 5 varieties) were identified. The plant with the highest appearance rate in the communities was Humulus japonicus Siebold & Zucc. In addition, annual herb species, including Rumex crispus L., Bromusjaponicus Thunb., Erigeron annuus (L.) Pers., and Artemisia indica Willd. were frequently observed to be growing in the secondary grassland. Naturalized plants were surveyed in the 38 taxa; the urbanization index was 10.3% and the naturalized index was 24.8%. Plant communities were largely classified into submerged vegetation, floating and floating-leaved vegetation, annual and biennial vegetation, perennial herb vegetation, and woody vegetation. The distribution of plant communities reflecting various habitats, including the lentic and lotic zone maintaining a constant water depth, littoral zone experiencing intermittent water level fluctuations, and dry floodplain environment was also confirmed. Overall, plant community development plays an important role in the habitat for wild animals; therefore, it is expected to positively impact biodiversity enhancement.

Effect of Pruning on the Yield and Fruit Quality of 'Godongsi' Persimmon (전정처리가 감나무 '고동시'의 수확량과 과실품질에 미치는 영향)

  • Hui-Won, Yoo;Chul-Woo, Kim;Seong-In, Park;Uk, Lee
    • Journal of Korean Society of Forest Science
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    • v.111 no.4
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    • pp.557-566
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    • 2022
  • This study aimed to obtain basic data on high-quality fruit production and efficient cultivation of persimmon cultivar "Godongsi". In the second year of pruning, the number of fruits per tree according to the number of fruit-bearing mother shoots (300, 400, 500, and 700 [control] treatments) was 676.3, 786.0, 866.7, and 1238.7, respectively. Persimmon cultivar "Godongsi" had a decrease in number of fruits per tree and increase in fruit weight as the pruning intensity increased. The average fruit weight in the 300 treatment group was 212.2 g, which was 1.5 times heavier than that in the control. For a high-quality dried persimmon processing, the rate of grade L fruits (170 g<x≤200 g) and over according to the number of fruit-bearing mother shoots was 86.9%, 48.6%, 33.5%, and 8.1%, respectively. The fruit grade of the pruned treatment group was significantly higher than that of the control. Based on the selling price of dried persimmon, the production amount was significantly higher in the 300 treatment than that in the control. When there were many fruits per tree, there was a decrease in quality and increase in cost of harvesting and processing. In conclusion, pruning is essential to increase the fruit quality (fruit weight and grade) of persimmon cultivar "Godongsi". Moreover, the suitable number of fruit-bearing mother shoots was 300.

Effect of SAL5 on chronic ethanol-induced fatty liver model (흰쥐에서 SAL5의 알코올성 지방간 형성에 미치는 영향)

  • Kim, Bok-Kyu;Yang, Won-Kyung;Park, Yang-Chun;Jung, Ga-Young;Shin, Eun-Ju;Do, Seon-Gil;Kim, Seung-Hyung
    • The Korea Journal of Herbology
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    • v.33 no.1
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    • pp.17-26
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    • 2018
  • Objective : In this study, we investigated the effect of SAL5(mixing extracts of Schisandra chinensis Baillon, Artemisia capillaris Thunb., and Aloe vera Linne) on chronic ethanol-induced fatty liver model. Methods : Sprague-Dawley male rats were fed Liber-DeCarli (normal), ethanol liquid diet (control), SAL5 (200 mg/kg). We administrated the SAL5 on chronic ethanol-induced fatty liver model for 5 weeks. We measured alkaline phosphtase (ALP), alanine transminase (ALT), aspartate transminase (AST) and ${\gamma}-glutamyl$ transpeptase (${\gamma}-GTP$) in serum and triglyceride (TG), superoxide dismutase (SOD), catalase, glutathione (GSH) and malondialdehyde (MDA) level in liver. Liver histopathology was examined by Hematoxylin-eosin and Oil red O staining of the fixed liver tissues. Real-time PCR was performed to measure the mRNA expression of inflammatory cytokines and MMP-2, MMP-9. Results : SAL5 administration resulted in significantly decreased liver marker enzymes activities of alanine transminase (ALT), ${\gamma}-glutamyl$ transpeptase (${\gamma}-GTP$) in serum and triglyceride (TG) activities in liver. The control group decreased the activities of superoxide dismutase (SOD), catalase (CAT) with the reduced level of glutathione (GSH) in liver. On the other hand, SAL5 group increased the activities of SOD, CAT and the level of GSH. SAL5 delayed the development of an alcoholic fatty liver by reversing fat accumulation in the liver, as evidenced in histological observations. The gene expression of mRNA were significantly decreased at the $IL-1{\beta}$, $TNF-{\alpha}$, NOS-II and MMP-2 by SAL5. Conclusions : These results indicate that SAL5 might have protective effect chronic ethanol-induced fatty liver models.

Antioxidant activity of ten Lamiaceae plant seed extracts (10종 꿀풀과(Lamiaceae) 식물 종자 추출물의 항산화 활성)

  • Kim, JunHyeok;Lee, Hee Ho;Park, Chung Youl;Kim, Hyun Min;Jung, Young Ho;Kim, Sae Hyun;Na, Chae Sun
    • Journal of Applied Biological Chemistry
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    • v.65 no.3
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    • pp.121-128
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    • 2022
  • This study explored plant-derived natural antioxidants by evaluating the antioxidant activity of Lamiaceae plant seed extracts. Plants with the percentage of filled seeds at or above 90% and seed germination at or above 50% were selected. Of the ten species studied, the total phenolic content of the seeds was high in the species Phlomis umbrosa Turcz. (6.2 mg GAEs/g of seeds) and Elsholtzia ciliata (Thunb.) Hyl. (4.5 mg GAEs/g of seeds). The total flavonoid content of the seeds was high in E. ciliata (1.0 mg QEs/g of seeds) and P. umbrosa (0.6 mg QEs/g of seeds). Based on the EC50 value of the seed extracts, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity was high in the seeds of the plants E. ciliata (27.5 ㎍/mL), Mosla dianthera (Buch.-Ham. ex Roxb.) Maxim. (29.2 ㎍/mL), and Prunella vulgaris var. lilacina Nakai (33.3 ㎍/mL). In addition, 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity was high in P. vulgaris var. lilacina (25.6 ㎍/mL), E. ciliata (25.9 ㎍/mL), and M. dianthera (27.6 ㎍/mL) seeds. The ferric reducing antioxidant power of the seed extracts was high in P. vulgaris var. lilacina (2910.4 µM Fe(II)/g of extract), E. ciliata (2836.2 µM Fe(II)/g of extract), and M. dianthera (2754.4 µM Fe(II)/g of extract). According to the cluster analysis based on antioxidant activity, the seeds of the ten species were classified into three groups, from group 1 with low antioxidant activity to group 3 with high antioxidant activity; E. ciliata, M. dianthera, and P. vulgaris var. lilacina were classified as group 3.

Comparison of the Effectiveness of Herbal Medicine Eye Drops on Conjunctivitis : A Systematic Literature Review and Meta-Analysis (결막염에 대한 한방 점안액의 효과 비교 : 체계적 문헌 고찰과 메타 분석)

  • Lim, Hui-Yeong;Kim, Jee-Hee;Ko, Woo-Shin;Yoon, Hwa-Jung
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.35 no.3
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    • pp.48-65
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    • 2022
  • Objectives : This study was conducted to find out the effectiveness and safety of herbal medicine eye drops on conjunctivitis. Methods : We searched randomized controlled trials on conjunctivitis through 10 DBs from the start to April 27, 2022. Study collection and data extraction, and evaluation of risk of bias were conducted by two independent researchers. The evaluation of the risk of bias in included RCTs was carried out by using Cochrane risk-of-bias tool. And the data synthesis was conducted by using Review Manager(RevMan, ver.5.4). Results : Total of 106 studies are researched and 7 studies of them are finally included. 1. The herbal medicine eye drops are more effective on than the western medicine eye drops, but the heterogeneity was very high. So we conducted sensitivity analysis and compared to the herbal medicine eye drops and the western medicine eye drops, the total effective rate was higher in the herbal medicine eye drops and the heterogeneity was somewhat reduced. 2. In the subgroup analysis, Fufang Xiongdan eye drops and Houttuynia cordata Thunb eye drops are more effective than the western medicine. It may represent substantial heterogeneity. 3. In the subgroup analysis, the herbal medicine eye drops are more effective than antibiotic and antiviral eye drops on acute bacterial conjunctivitis, but showed high heterogeneity. On the other hand, the herbal medicine eye drops are more effective than antibiotic and antiviral eyed drops on acute viral conjuctivitis and showed low heterogeneity. 4. In the subgroup analysis, we compared the effectiveness of herbal and western medicine eye drops according to total effectiveness rate evaluation methods. When comparing the studies calculating the total effective rate by the sum of 'Cured', 'Significantly Impaired', and 'Improved', the effective rate of the herbal medicine eye drops was higher than that of the western medicine eye drops, and the heterogeneity was very low. 5. In most studies, side effects of herbal medicine eye drops were absent or mild. Conclusions : This study shows that herbal medicine eye drops may be more effective than western medicine eye drops and have safety. But further researches are needed to resolve the heterogeneity of this study.

Studies on the Variation of Vegetation and Rice Root Formation Accompanied with the Desaltation at the Reclaimed! Tidal Fields (간척지의 제염정도에 따른 식생의 변이의 수도근모형성에 관한 연구)

  • Kwon. H.J.;Chung, W.I.;Cho, J.Y.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.3
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    • pp.305-309
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    • 1983
  • To know the effect of desaltation in the reclaimed tidal fields on the succession of vegetation and on the root hair formation of paddy rice, reclaimed fields in Kang Hwa Island, Nam-Yang Bay and Ke-Hwa Island were investigated during summer crop season in 1982. The obtained results can be summarized as followings. l) In the highly salty and water logged areas of reclaimed tidal fields, the first dominating species of higher plant were observed to be Salicornia herbacea L. and Suaeda japonica Makino. With the continued desaltation, Chemopodium virgatum Thumb. occurred in the vegetation. After this transition, the dominating species were composed of Scirpus maitimus L. and Phragmites communis Trin. At the S. maitimus and P. communis dominating salinity level, rice cultivation was safe from the salt damage. 2) In the water logged area, Artemisia capillaria Thunb. and Aster tripolium L. took the place of dominating species after S. herbacea and S. japonica. At this salinity level, graminaceous weeds began to immigrate. 3) In dry areas, Suaeda asparagoides Makino and Suaeda maritima were the first appearing dominant species. Atriplex subcordata Kitakawa was also observed in sucy dry areas, but colony formation was not observed. 4) Plants immigrated slowly into dry areas from the already vegetated water logged areas with the continuation of desaltation. 5) The high soil salinity level affected the root hair formation of rice by reducing both the rate of root hair formation and the length of root hairs.

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