• Journal of the Korean Applied Science and Technology
• /
• v.38 no.6
• /
• pp.1370-1382
• /
• 2021

Previous studies have been conducted on domestic materials as a single extract. Research on complex mixtures for maximizing plant characteristics by individual extraction and potential interference with effects is insufficient. Therefore, this study confirmed the GC-MSD according to the extraction of essential oils for Agastache rugosa O. Kuntze(AR), Pinus densiflora Sieb. et Zuccarini(PD), Curcuma longa, Curcuma domestica(CC), Zingiber officinale Roscoe(ZR), Foeniculum vulgare Miller(FV), and Citrus medica L. var. sarcodactylis Swingle(CS). The cytotoxicity, antioxidant, and anti-inflammatory properties of the blending oil were confirmed to confirm its potential as a cosmetic material. As a result of analyzing GC-MSD aroma components, the main components were estragole of AR, à-Pinene for PD, Zingiberene for CC and ZR, Anethole from FV, and D-Limonene for CS. At a concentration of 100 uL/mL with no confirmed cytotoxicity, NO production was inhibited by 70.62%, DPPH radical scavenging activity was 64.03%, and ABTS radical scavenging activity was 89.55%. Through this, blended essential oil suggests the possibility of useful application as a raw material with antioxidant and anti-inflammatory effects in the cosmetic and food industries.

• Korean Journal of Food Science and Technology
• /
• v.51 no.4
• /
• pp.301-308
• /
• 2019

The antioxidant properties of Eriobotrya japonica leaf extract were investigated using DPPH and ABTS radical scavenging assay. The 80% ethanol extract of leaves ($IC_{50}$ values for DPPH and ABTS were 13.9 and $10.9{\mu}g/mL$, respectively) and young leaves ($IC_{50}$ values for DPPH and ABTS were 20.7 and $17.3{\mu}g/mL$, respectively) showed high radical scavenging activity. Additionally, the quantitative method for estimation of ellagic acid and chlorogenic acid from E. japonica leaves was optimized by HPLC/DAD. This method showed high linearity of the calibration curve with a coefficient of correlation ($R^2$) equal to 0.999. The LOD values for ellagic acid and chlorogenic acid were 2.35 and $0.73{\mu}g/mL$, respectively, whereas LOQ values were 7.13 and $2.22{\mu}g/mL$, respectively. Recovery of the two compounds was 99.7-108.0% with RSD values less than 5.31%. These results suggest that 80% ethanol extract of E. japonica leaves could serve as a potential source of natural antioxidant for us in various industrial applications.

• Journal of the Korean Applied Science and Technology
• /
• v.37 no.5
• /
• pp.1180-1189
• /
• 2020

This study attempted to investigate the physiological activities of Passiflora caerulea extract and provide basic data needed to verify its availability as an inner beauty material. To examine its anti-oxidant, anti-inflammatory and anti-aging effects, the followings were measured: total polyphenol contents, total flavonoid contents, DPPH radical scavenging activity, cytotoxicity and NO inhibition effects in RAW 264.7 macrophage cells and HDF cells and MMP-1 inhibitory effects in HDF cells. The results found the followings: First, polyphenol and flavonoid contents were great with 157 mg/g and 173.5 mg/g respectively at 10mg/mL, and DPPH radical scavenging activity was confirmed. In RAW 264.7 macrophage cells and HDF cells, no cytotoxicity was observed for Passiflora caerulea extract. In addition, 69.3% anti-inflammatory effects were found at 100 ㎍/mL through NO inhibition in RAW 264.7 macrophage cells. In addition, a significant inhibition of MMP-1 expression in HDF cell was confirmed. The above results confirmed the anti-oxidant and anti-inflammatory effects of Passiflora caerulea extract, low toxicity on skin cells and its anti-aging effects by inhibiting MMP-1 expression. Therefore, it appears that Passiflora caerulea extract would be available as diverse inner beauty materials.

• Biomedical Science Letters
• /
• v.13 no.1
• /
• pp.25-32
• /
• 2007

The purpose of this study was to investigate the effect of the high voltage pulsed Current (HVPC) stimulation on the healing rate and the proliferative activity of keratinocytes and IGF-I mRNA expression of an incisional wound in rat skin. Twenty male Sprague-Dawley rats ($265{\sim}290g$) were randomly divided into HVPC (n=10) and control group (n=10). Rats received 10 mm length of full-thickness incision wound on the back under the anesthesia. The HVPC group received electrical stimulation with a Current intensity of 50 V at 100 pps for a duration of 30 minutes, while the control group was given the same treatment without electricity for a week. Polarity was negative in first three days and positive thereafter. The wound length was measured and evaluated as percentage. The mean number of nucleolar organizer regions (NORs) per nucleus and level of IGF-I mRNA expression were calculated. The mean percent of wound closure were $51.17{\pm}17.76%$ and $80.71{\pm}11.91%$, respectively, in the sham treated control and HVPC stimulated groups (t=-4.308, P<0.001). The mean NOR number per nucleus of the keratinocytes in the control and HVPC group were $1.85{\pm}0.20$ and $2.70{\pm}0.23$, respectively (t=8.638, P<0.001). The IGF-I mRNA level were $0.76{\pm}0.44$ and $1.32{\pm}0.41$, respectively, in the control and HVPC stimulated wounds (t=2.906, P<0.01). There was a positive correlation between the mean NOR number per nucleus and IGF-l mRNA level with a Pearson product moment correlation coefficient of 0.72 (P<0.05). These findings suggest that the HVPC may activate the rRNA of the basal keratinocytes and upregulate the IGF-I mRNA levels by alteration of the electrical environment, and it may increase proliferative activity of the keratinocytes in the skin wound of the rat.

• Applied Microscopy
• /
• v.34 no.2
• /
• pp.145-157
• /
• 2004

The aim of the present study was to examine in detail, both at light and electron microscopical levels, the morphological variations in ameloblast of the fetal rat incisor enamel organ. Rats were started on distilled water at the beginning of pregnancy. The pups were sacrificed 11 days after delivery and animals were perfused intravascularly with glutaraldehyde and the incisors were removed. To examine on the ultrastructure of the ameloblast, the study employed primary light microscopy but electron microscopy was used to clarify some of the light microscopic finding. Longitudinal sections through the incisors of the rat show a continuous layer of ameloblasts on the labial surface of the tooth. This layer contains the entire sequence of developmental stages in enamel production. The ameloblast layer was divided into three main zones: 1) Presecretory zone, region of ameloblasts facing pulp. 2) Secretory zone, region of inner and outer enamel secretion. 3) Maturation zone, region of reduced ameloblasts. In particularly, the present study has shown that two distinctively different types of ameloblasts appear in the enamel organ during enamel maturation in the rat incisor. These two types have been designated ruffle-ended ameloblasts (rAB) and smooth-ended ameloblasts (sAB). The fluoride produces marked alteration in the fine structure of ameloblast from teeth of young rats, such as large confluent distensions of the endoplasmic reticulum and swelling of isolated mitochondria. This experimental data suggested that exposure prolonged of animal to high level of fluoride appears to induce a few dramatic changes in the normal appositional growth and initial mineralization of enamel created during amelogenesis.

• Applied Microscopy
• /
• v.39 no.3
• /
• pp.237-243
• /
• 2009

Chinese minnow, Rhynchocypris oxycephalus is a teleost belonging to Leuciscinae, Cyprinidae. The oogenesis and ultrastructure of egg envelope in Chinese minnow were investigated by light and electron microscopes. The ovary was of white yellowish and ellipsoidal shape with the major axis 30 mm and the minor axis 7mm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The basophilic substance of cytoplasm was changed to acidic. In case of matured egg, thickness of egg envelope and size of egg were increased. The yolk vesicles were changed to yolk mass in accordance with development. The outer surface of egg envelope was covered by microvilli-structures, and had a micropyle on the area of animal pole. Egg envelope consisted with 2 layers, an adhesive outer layer with microvilli-structures and fibrillar inner layer. In conclusion, the oogenesis of Chinese minnow was characterized by the increase in cell size, the formation and accumulation of yolk, and the decrease of basophilic substance in the cytoplasm. The oogenesis of Chinese minnow seems to share common patterns in Cyprinidae, but these ultrastructural unique characters of egg envelope can be utilized in taxonomy of teleost.

• Applied Microscopy
• /
• v.38 no.4
• /
• pp.315-319
• /
• 2008

Glow-light tetra, Hemigrammus erythrozonus is a teleost belonging to Characidae. The natural habitate of this fish is the wild in the Essequibo river, Guyana and South America. The oogenesis of glow-light tetra was investigated by light microscopy to compare with those of other families. A pair of ovary was located between swim bladder and intestines. The ovary was of white color and ellipsoidal shape with the major axis 11 mm and the minor axis 4 mm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The amount of basophilic substance was decreased. In case of matured egg, thickness of egg envelope and size of egg were increased, basophilic substance was distributed in only around the egg envelope. The yolk vesicles were changed to yolk mass in accordance with development. In conclusion, the oogenesis of glow-light tetra, Hemigrammus erythrozonus was characterized by the increase in cell size, the formation and accumulation of yolk, and the decrease of basophilia a in the cytoplasm. The oogenesis of glow-light tetra seems to share common patterns in Characidae, teleost and have a similar pattern with other teleost.

• Applied Microscopy
• /
• v.41 no.3
• /
• pp.155-161
• /
• 2011

Three spot gourami (Trichogaster trichopterus Pallas, 1770) is a teleost belonging to Belontiidae. The oogenesis of three spot gourami was investigated by light microscope. The ovary was of light peach color and ellipsoidal shape with the major axis 2 cm and the minor axis 1 cm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocyte, lipid droplets were distributed only in the marginal area first, than at nuclear envelope near. In secondary oocyte, the egg envelope was formed and yolk vesicles was formed in the marginal area. The basophilic substance of cytoplasm was changed to acidic. In case of matured egg, thickness of egg envelope and size of egg were increased. The yolk vesicles were changed to yolk mass in accordance with development. The fertilized eggs were the colorless, transparent, spherical, adhesive and pelagic type. A large oil droplet was located in vitelline membrane of the fertilized egg. In conclusion, the oogenesis of three spot gourami was characterized by the increase in cell size, the formations of lipid droplets and yolk, the decrease of basophilic substance in the cytoplasm, and formation of one large oil droplets.

• Applied Microscopy
• /
• v.37 no.2
• /
• pp.135-142
• /
• 2007

This research investigated morphological changes of ovarian follicle according to dose of irradiation when adult mice were exposed to X-rays from 6 MeV LINAC. At day 3 after irradiation of 200 cGy, 400 cGy and 600 cGy X-ray to the hole body of mice, the ovaries collected and stained with TUNEL. The normal follicles and atretic follicles were identified to apoptosis by the staining with TUNEL. In the atretic follicles of the normal ovary, the apoptotic bodies were well appeared and stained brown color. Almost of the follicles following irradiation are stained with TUNEL, but the sensitivity of reaction is weaker than that in irradiation of 400 cGy and 600 cGy X-ray. The granulosa cells of the radiated normal follicle by 400 cGV are shown brown color. In this stage, the nucleus of granulosa cells in the atrectic follicles are condensed and picknotic feature. The size of the radiated follicle by 600 cGy are decreased than the normal follicles. The atropic follicles are filled with apoptotic bodies which change of granulosa cells and theca cells by influence of X-ray. All of cell in the follicles are strongly positive stained with TUNEL by irradiation of 600 cGy.

• Applied Microscopy
• /
• v.40 no.1
• /
• pp.1-8
• /
• 2010

The ultrastructure of spermatogenesis and sperm in Chinese minnow, Rhynchocypris oxycephalus belonging to Leuciscinae was investigated by light and electron microscopes. The whitish testis was located between intestine and air bladder. The size of testis was major axis 2.3 cm, minor axis 6 mm. The testis contained numerous testicular cysts, and spermatogenesis was non-synchronized in these testicular cysts. In the case of spermatogonium, the nucleus was comparatively large ellipsoidal, and mitochondria showed a marked development. The size of primary spermatocyte was smaller than that of spermatogonia, and secondary spermatocyte was smaller than primary spermatocyte. The chromatin of spermatocyte was highly condensed according to their development. The nucleus with electron-dense was round shape. In spermiogenesis, flagella started to be formed and chromatin was more condensed. The mitochondria were rearranged in a middle piece. The sperm was formed by loss of cytoplasm. The head of mature sperm was a spherical shape and have not acrosome. The microtubules of flagella were arranged 9+2 structure. Also, the tail of sperm have not lateral fins.