Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
권호 표지

The Spermatogenesis of Chinese minnow, Leuciscinae, Teleostei

경골어류 황어아과 버들치의 정자형성과정

  • Kim, Dong-Heui (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Chang, Byung-Soo (Department of Cosmetology, Hanseo University) ;
  • Kim, Wan-Jong (Department of Life Science, Soonchunhyang University) ;
  • Lee, Myeong-Seon (Department of Genetic Engineering, Cheongju University) ;
  • Teng, Yung-Chien (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Kim, Seok (Department of Environmental Medical Biology, Central Research Lab, Wonju College of Medicine, Yonsei University) ;
  • Lee, Kyu-Jae (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University)
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소) ;
  • 장병수 (한서대학교 보건학부 피부미용학과) ;
  • 김완종 (순천향대학교 자연과학대학 생명과학과) ;
  • 이명선 (청주대학교 이공대학 유전공학과) ;
  • 등영건 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소) ;
  • 김석 (연세대학교 원주의과대학 환경의생물학교실, 중앙연구실) ;
  • 이규재 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소)
  • Received : 2010.01.05
  • Accepted : 2010.03.20
  • Published : 2010.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The ultrastructure of spermatogenesis and sperm in Chinese minnow, Rhynchocypris oxycephalus belonging to Leuciscinae was investigated by light and electron microscopes. The whitish testis was located between intestine and air bladder. The size of testis was major axis 2.3 cm, minor axis 6 mm. The testis contained numerous testicular cysts, and spermatogenesis was non-synchronized in these testicular cysts. In the case of spermatogonium, the nucleus was comparatively large ellipsoidal, and mitochondria showed a marked development. The size of primary spermatocyte was smaller than that of spermatogonia, and secondary spermatocyte was smaller than primary spermatocyte. The chromatin of spermatocyte was highly condensed according to their development. The nucleus with electron-dense was round shape. In spermiogenesis, flagella started to be formed and chromatin was more condensed. The mitochondria were rearranged in a middle piece. The sperm was formed by loss of cytoplasm. The head of mature sperm was a spherical shape and have not acrosome. The microtubules of flagella were arranged 9+2 structure. Also, the tail of sperm have not lateral fins.

경골어류 잉어과(Cyprinidae), 황어아과(Leuciscinae)에 속하는 버들치(Rhynchocypris oxycephalus)의 정자형성과정과 정자의 형태를 광학현미경과 전자현미경을 이용하여 관찰하였다. 버들치의 정소는 부레와 창자사이에 위치하고 있었고 장축 약 2.3 cm, 단축 약 6 mm 정도로 팽창된 긴방추형이었고 흰색을 띠고 있었다. 정자형성과정은 정소낭(testicular cyst)에서 이루어졌으며, 각 정소낭 내에서 다양한 분화시기의 생식세포가 분포하고 있었다. 정원세포는 전자밀도가 매우 낮고 미토콘드리아의 발달이 현저하였다. 제1정모세포는 원형으로 정원세포보다 크기가 작았고 제2정모세포는 제1정모세포보다 더 작아졌고 핵의 전자 밀도는 더 높았다. 정세포의 초기발달시기에는 세포의 크기가 정모세포보다 작아졌고 두부의 전자밀도가 높아졌으며 편모가 형성되기 시작하였고 미토콘드리아는 핵 주변에 분포하였다. 정자 완성과정 말기에는 핵의 염색질 응축이 뚜렷하였으며 핵은 세포질 한쪽에 치우쳐 있었고, 미토콘드리아는 편모 주변에 집중되었다. 완전히 성숙한 정자의 경우 두부형태는 구형이었고 두부에서 첨체는 관찰되지 않았으며 편모의 미세소관 배열은 9+2구조를 이루고 있었다. 또한 편모 양쪽으로 lateral fin은 관찰되지 않았다.

Keywords

References

  1. Bayarri MJ, Zanuy S, Yilmaz O, Carrillo M: Effects of continuous light on the reproductive system of European sea bass gauged by alterations of circadian variations during their first reproductive cycle. Chronobiol Int 26(2) : 84-99, 2009.
  2. Billard R: Spermiogenesis in the rainbow trout (Salmo gairdneri): An ultrastructural study. Cell Tissue Res 233 : 265-284, 1983.
  3. Brusle S: Ultrastructure of spermatogenesis in Liza aurata Risso, 1810 (Teleostei, Mugilidae). Cell Tissue Res 217 : 415-424, 1981.
  4. Chung EY: Ultrastructure of germ cells, the Leydig cells, and Sertoli cells during spermatogenesis in Boleophthalmus pectinirostris (Teleostei, Perciformes, Gobiidae). Tissue and Cell 40 : 195-205, 2008. https://doi.org/10.1016/j.tice.2007.11.003
  5. Deung YK, Kim DH, Reu DS: Ultrastructure of gametes in the threespine stickleback, Gasterosteus aculeatus aculeatus. Kor J Electron Microscopy 29(2) : 177-178, 1999 (Korean).
  6. Gwo JC, Gwo HH: Spermatogenesis in the black porgy Acanthopagrus schlegeli (Teleostei: Perciformaes: Sparidae). Mol Rep Dev 36 : 75-83, 1993. https://doi.org/10.1002/mrd.1080360111
  7. Kim DH, Deung YK, Kim WJ, Reu DS, Kang SJ: Comparative ultrastructures of the fertilized egg envelopes from three-spot gourami, pearl gourami and marble gourami, Belontiidae, Teleost, Kor J Electron Microscopy 29(3) : 343-351, 1999 (Korean).
  8. Kim DH, Lee KJ, Kim S, Teng YC: The Spermatogenesis of Coreoleuciscus splendidus, Cyprinidae, Teleost. Kor J Microscopy 39(3) : 227-236, 2009a (Korean).
  9. Kim DH, Reu DS, Deung YK: An ultrastructural study on the spermatogenesis of Xiphophorus maculatus. Kor J Electron Microscopy 33(4) : 267-274, 2003 (Korean).
  10. Kim IS, Park JY: Freshwater fishes of Korea, Kyo-Hak Publishing Co., Ltd., 166, 2002 (Korean).
  11. Kim JG, Kim DH, Reu DS: A Study on the Reproductive Cells in Testes of Microphysogobio yaluensis. Kor J Microscopy 39(3) : 245-252, 2009b (Korean).
  12. Lee KJ, Chang BS, Teng YC, Kim S, Song MS, Joo KB, Kim DH: The Spermatogenesis of Cichlasoma managuensis, Cichlidae, Teleost. Kor J Microscopy 39 (3) : 219-226, 2009 (Korean).
  13. Miura T, Yamauchi K, Nagahama Y, Takahashi H: Introduction of permatogenesis in male Japanese eel, Anguilla japonica, by a single injection of human chorionic gonadotropin, Zool Sci 8 : 63-73, 1991.
  14. Rossouw GJ, Vanessen LD: Spermatogenesis in the male whitespotted houdshark, Mustelus palubes Smith. South African J Sci 89 : 244-246, 1993.
  15. Sprando RL, Russell LD: Spermiogenesis in the bluegill (Lepomis macrohirus): A study of cytoplasmic events including cell volume changes and cytoplasmic elimination. J Morphol 198: 165-177, 1988. https://doi.org/10.1002/jmor.1051980204
  16. Todd PR: Ultrastructure of the spermatozoa and spermiogenesis in New Zealand freshwater eels (Anguillidae), Cell Tissue Res 171 : 224-232, 1976.
  17. van Deurs B, Lastein U: Ultrastructure of the spermatozoa of the teleost, Pantodon buchholzi Peters, with particular reference to the midpiece. J Ultrastruct Res 42(5) : 517-533, 1973. https://doi.org/10.1016/S0022-5320(73)80024-3
  18. Wolenski JS, Hart NH: Scanning electron microscope studies of sperm incorporation into the zebrafish egg. J Exp Zool 243 : 259-273, 1987. https://doi.org/10.1002/jez.1402430211
  19. Yoneyama H, Iwasawa H: Annual changes in the testis and accessory sex organs of the bullfrog Rana catesbeiana. Zool Sci 2 : 229-237, 1985.