Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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The Oogenesis of Glow-light Tetra, Characidae, Teleost

경골어류 카라신과 glow-light tetra의 난자형성과정

  • Lee, Kyu-Jae (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Chang, Byung-Soo (Department of Cosmetology, Hanseo University) ;
  • Teng, Yung-Chien (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Kim, Dong-Heui (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University)
  • 이규재 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 장병수 (한서대학교 보건학부 피부미용학과) ;
  • 등영건 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실)
  • Published : 2008.12.31
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Abstract

Glow-light tetra, Hemigrammus erythrozonus is a teleost belonging to Characidae. The natural habitate of this fish is the wild in the Essequibo river, Guyana and South America. The oogenesis of glow-light tetra was investigated by light microscopy to compare with those of other families. A pair of ovary was located between swim bladder and intestines. The ovary was of white color and ellipsoidal shape with the major axis 11 mm and the minor axis 4 mm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The amount of basophilic substance was decreased. In case of matured egg, thickness of egg envelope and size of egg were increased, basophilic substance was distributed in only around the egg envelope. The yolk vesicles were changed to yolk mass in accordance with development. In conclusion, the oogenesis of glow-light tetra, Hemigrammus erythrozonus was characterized by the increase in cell size, the formation and accumulation of yolk, and the decrease of basophilia a in the cytoplasm. The oogenesis of glow-light tetra seems to share common patterns in Characidae, teleost and have a similar pattern with other teleost.

조기어강 (Actinopterygii), 카라신목 (Characiformes), 카라신과(Characidae)에 속하는 담수산 경골어류에 속하는 Glow-light tetra (Hemigrammus erythrozonus)의 난자형성과정을 다른 어류와의 차이점을 확인하기 위하여 광학현미경으로 관찰한 결과 난원세포의 세포질은 호염기성이었고 핵 내에 다수의 이형염색질들이 분포하고 있었다. 제1난모세포의 경우 난황포가 단지 난세포 가장자리에만 배열되어 있었고 난막의 형성은 관찰되지 않았다. 제2난모세포에서는 난막이 형성되었고 제1난모세포에 비해서 난황포가 점점 핵 쪽으로 증식된 경향을 보였다. 발생이 진행됨에 따라서 호염기성 물질들은 점점 감소하는 경향을 보였고 후에 난막 주위에만 국한적으로 분포하였으며, 난막의 두께와 난자의 크기는 점점 증가되었다. 이상과 같이 glow-light tetra의 난자형성과정은 난세포의 크기 증가, 난황낭의 축적, 염기성 물질의 감소, 난막발달 및 두께의 증가로 요약될 수 있으며 일반적인 다른 과의 담수경골어류와 큰 차이는 없는 것으로 확인되었다. 본 연구는 광학현미경상의 조직학적 결과이므로 앞으로 정확한 난자형성과정을 단계적으로 확인하기 위한 투과전자현미경을 이용한 미세구조적 연구가 필요하다.

Keywords

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