Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Availability of Passiflora Caerulea Extract as Inner Beauty Material

이너뷰티 소재로서의 시계꽃 추출물의 활용 가능성

  • Lee, Jae-Nam (Department of Cosmetology, Graduate School of Engineering, Konkuk University) ;
  • Kem, Young-Sam (Dept. of Image Industry, Graduate School of Engineering, Konkuk University)
  • 이재남 (건국학교 산업대학원 향장학과) ;
  • 김영삼 (건국학교 산업대학원 이미지산업학과)
  • Received : 2020.09.30
  • Accepted : 2020.10.29
  • Published : 2020.10.31
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Abstract

This study attempted to investigate the physiological activities of Passiflora caerulea extract and provide basic data needed to verify its availability as an inner beauty material. To examine its anti-oxidant, anti-inflammatory and anti-aging effects, the followings were measured: total polyphenol contents, total flavonoid contents, DPPH radical scavenging activity, cytotoxicity and NO inhibition effects in RAW 264.7 macrophage cells and HDF cells and MMP-1 inhibitory effects in HDF cells. The results found the followings: First, polyphenol and flavonoid contents were great with 157 mg/g and 173.5 mg/g respectively at 10mg/mL, and DPPH radical scavenging activity was confirmed. In RAW 264.7 macrophage cells and HDF cells, no cytotoxicity was observed for Passiflora caerulea extract. In addition, 69.3% anti-inflammatory effects were found at 100 ㎍/mL through NO inhibition in RAW 264.7 macrophage cells. In addition, a significant inhibition of MMP-1 expression in HDF cell was confirmed. The above results confirmed the anti-oxidant and anti-inflammatory effects of Passiflora caerulea extract, low toxicity on skin cells and its anti-aging effects by inhibiting MMP-1 expression. Therefore, it appears that Passiflora caerulea extract would be available as diverse inner beauty materials.

본 연구는 시계꽃 추출물의 생리활성 효능을 확인하여 이너뷰티 소재로서의 활용 가능성을 검증하여 기초자료를 제공하고자 한다. 실험 방법으로는 항산화, 항염증, 항노화 효과를 확인하기 위하여 총폴리페놀 함량, 총 플라보노이드 함량, DPPH radical 소거능, RAW 264.7 대식세포와 HDF 세포 내에서의 세포 독성 및 NO 생성 억제 효과, HDF 세포에서의 MMP-1의 발현 억제능 효과를 측정하였다. 실험결과, 시계꽃 추출물의 폴리페놀함량과 플라보노이드 함량은 10 mg/mL의 농도에서 각 157 mg/g, 173.5 mg/g의 우수한 함량을 확인하였고, DPPH radical 소거능을 확인하였다. RAW 264.7 대식세포와 HDF 세포에서는 시계꽃 추출물에 대해 세포독성은 나타나지 않았다. 또한 RAW 264.7 대식세포에서의 NO 생성억제를 통해 100 ㎍/mL에서 69.3%의 항염증 효과를 확인하였으며, HDF 세포에서 MMP-1의 발현 억제능이 유의하게 억제되는 것을 확인하였다. 이상과 같이 본 연구의 시계꽃 추출물은 항산화 효과와 항염증 효과, 피부세포에 대한 낮은 독성, MMP-1의 발현 억제를 통한 항노화 효과가 확인됨에 따라 다양한 이너뷰티 소재로서의 활용이 가능할 것으로 사료된다.

Keywords

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