• Journal of Microbiology and Biotechnology
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• v.32 no.8
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• pp.1054-1063
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• 2022

Trehalose is a non-conventional sugar with potent applications in the food, healthcare and biopharma industries. In this study, trehalose was synthesized from maltose using whole-cell Pseudomonas monteilii TBRC 1196 producing trehalose synthase (TreS) as the biocatalyst. The reaction condition was optimized using 1% Triton X-100 permeabilized cells. According to our central composite design (CCD) experiment, the optimal process was achieved at 35℃ and pH 8.0 for 24 h, resulting in the maximum trehalose yield of 51.60 g/g after 12 h using an initial cell loading of 94 g/l. Scale-up production in a lab-scale bioreactor led to the final trehalose concentration of 51.91 g/l with a yield of 51.60 g/g and productivity of 4.37 g/l/h together with 8.24 g/l glucose as a byproduct. A one-pot process integrating trehalose production and byproduct bioremoval showed 53.35% trehalose yield from 107.4 g/l after 15 h by permeabilized P. moteilii cells. The residual maltose and glucose were subsequently removed by Saccharomyces cerevisiae TBRC 12153, resulting in trehalose recovery of 99.23% with 24.85 g/l ethanol obtained as a co-product. The present work provides an integrated alternative process for trehalose production from maltose syrup in bio-industry.

• Wind and Structures
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• v.37 no.2
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• pp.79-94
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• 2023

This paper traces the drafting of the new EN 1991-1-4 Eurocode 1 - Actions on structures - Part 1-4: General actions - Wind actions within Mandate M/515 of the European Commission to CEN, for the evolution of structural Eurocodes towards their Second Generation. Work of the Project Team started in August 2017 and ended in April 2020, with delivery of a final draft for public enquiry. The revised document contains several modifications with respect to the existing 2005 version, and new sections were added, covering aspect not dealt with in the previous version. It has a renovated structure, with a main text limited in size and containing only fundamental material; all the remaining information, either normative or informative is arranged into thirteen annexes. Common to other Eurocode Parts, general requests from CEN were those of reducing the number of Nationally Determined Parameters and of enhancing the ease of use. More specific requests were those of (a) the drafting of a European design wind map, (b) improving wind models, (c) reviewing force and pressure coefficients, (d) reviewing the procedures for evaluation of the dynamic response, as well as (e) making editorial improvements aimed at a more user friendly document. The author had the privilege to serve as Project Team member for the drafting of the new document, and this paper brings his personal view concerning some general aspects of wind code writing, and some more specific aspects about the particular document.

• International conference on construction engineering and project management
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• 2015.10a
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• pp.236-240
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• 2015

The accelerating adoption of BIM (Building Information Modeling) is challenging collaboration practices established in the construction industry. The implementation of BIM involves changes in participants work, organization, processes and collaboration methods. Therefore there is a need to be able to measure effectively and accurately collaboration, in order to analyze and determine current practices and their performances in organizations (company, team project) as well as changes required. Previous researches scope from evaluating BIM maturity of an organization to BIM collaboration requirements but lack of proper tools and methods to analyze collaboration performances. This is especially true when it comes to evaluate the efficiency and collaboration performances of processes rather than systems or organizations. Thus this research aims to analyze systematically and comprehensively previous researches proposing diversified methods to evaluate BIM performances and collaboration. Furthermore it aims to suggest key indicators to evaluate collaboration performances of processes and project organizations. This research may contribute to better understanding of collaboration performances within organizations using BIM and further development of evaluation method for analyzing BIM design project.

• The Journal of Korean Medical History
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• v.36 no.2
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• pp.1-12
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• 2023

Immediately after liberation, Shin Kwang-ryul served as the director of the Shinbukcheong People's General Hospital, and defected to South Korea alone in December 1945 with hostility to the Soviet military government. Later, he joined the political operation team (政治工作隊) and was dispatched to the Sinbuk Office as a member of Hamgyeong-do's committee. However, after this was revealed, his wife was taken to the security and tortured to death. Later, when he learned about this, he left a Wolnam Yuseo (越南遺書) and decided to commit suicide. He left politics and started a new family while running a pharmacy. In 1950, he fled Dangjin, Chungnam, during the Korean War and opened an East Asian medical clinic. In 1955, he passed the Korean Medical Examination and opened Cheongpa Oriental Medicine Clinic in Asan, Chungcheongnam-do. In 1969, he ran an East Asian medical clinic in Dohwa-dong, Mapo, Seoul, and moved to Hongeun-dong in 1972 to open Hamnam Oriental Clinic. At this time he wrote a Cheongpa Pharmacy Summary (靑坡驗方要訣). In his later years, he treated poor patients for free, and he continued to work even though he was unwell due to a broken spine. He died in 1980 leaving behind a "proud mind".

• The Journal of Korean Academic Society of Nursing Education
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• v.30 no.1
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• pp.82-95
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• 2024

Purpose: This study aimed to identify the needs for the managerial competencies of nurse managers in general hospitals. Methods: A cross-sectional design was used. The participants were 203 nurse managers, 141 head nurses or unit managers, and 62 directors or team managers from 17 general hospitals. Data were collected by a self-administered questionnaire with 42 nursing management competencies and 181 behavioral indicators. Participants rated the importance and performance of each indicator. Data were analyzed using a paired t-test, independent t-test, importance-performance analysis, Borich's needs assessment model, and locus for focus model. Results: In all 42 competencies, importance was rated significantly higher than performance. The head nurses or unit managers had high demands for "human resource development," "performance management," and "information management," while the directors or team managers had high demands for "change management." Competencies in high demand in both groups were "development of nursing standards" and "self-management." Conclusion: Based on the results, it is necessary to select priorities and prepare educational content when developing educational programs to strengthen the nursing management competencies of general hospitals' nurse managers.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.4
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• pp.907-913
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• 2008

Taraxci Herba (TH; Pogongyoung in Korean) has been used in traditional oriental medicine for the treatment of various ailments. The biological activity of this plant is not yet evaluated systematically. This study was conducted to evaluate the inhibitory effects of TH on the production of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-activated Raw264.7 cells. The aim of the present work is to investigate a potential anti-inflammatory activity of TH. The Raw264.7 cells were cultured in DMEM medium for 24 h. After serum starvation for 12 h, the cells were treated with TH for 1 h, followed by stimulating NO production with LPS ($2{\mu}g/ml$). As result of this study, TH inhibited the levels of NO, PGE2, $TNF-{\alpha}$, IL-6 and $IL-1{\beta}$, and the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) activated by LPS. These inhibitory effects were mediated though the inhibition of phosphorylation of inhibitory kappa B ($I{\kappa}B$). These findings showed that TH could have some anti-inflammatory effects.

• Korean Journal of Materials Research
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• v.20 no.11
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• pp.559-569
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• 2010

The liquid-phase sintering method was used to prepare a glass lens forming core composed of SiC-$Al_2O_3-Y_2O_3$. Spark plasma sintering was used to obtain dense sintered bodies. The sintering characteristics of different SiC sources and compositions of additives were studied. Results revealed that, owing to its initial larger surface area, $\alpha$-SiC offers sinterability that is superior to that of $\beta$-SiC. A maximum density of $3.32\;g/cm^3$ (theoretical density [TD] of 99.7%) was obtained in $\alpha$-SiC-10 wt% ($6Al_2O_3-4Y_2O_3$) sintered at $1850^{\circ}C$ without high-energy ball milling. The maximum hardness and compression stress of the sintered body reached 2870 Hv and 1110 MPa, respectively. The optimum ultra-precision machining parameters were a grinding speed of 1243 m/min, work spindle rotation rate of 100 rpm, feed rate of 0.5 mm/min, and depth of cut of $0.2\;{\mu}m$. The surface roughnesses of the thus prepared final products were Ra = 4.3 nm and Rt = 55.3 nm for the aspheric lens forming core and Ra = 4.4 nm and Rt = 41.9 for the spherical lens forming core. These values were found to be sufficiently low, and the cores showed good compatibility between SiC and the diamond-like carbon (DLC) coating material. Thus, these glass lens forming cores have great potential for application in the lens industry.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.6
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• pp.717-729
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• 2013

Bioelectrical Impedance Analysis (BIA) is a non-invasive and low-cost technique that estimates body composition based on the distribution of water and electrolytes in the body by analyzing body's electrical responses to source voltages. In this work, we carried out a systematic literature review on BIA researches in traditional East Asian medicine (TEAM). For comparison, firstly we introduced the concept and principle of BIA, and offered a general overview of research trends in western medical perspectives. We searched through the databases of Oriental Medicine Advanced Searching Integrated System and DataBase Periodical Information Academic for the articles published between 1994 and 2013, with keywords such as 'BIA', 'bioelectrical impedance' and 'impedance'. Among the rough-searched 274 articles, we finally selected 21 articles appropriate to the intended research field. The selected articles were categorized into diagnosis in Sasang medicine, impedance analysis in meridian system, and change of body composition after taking herbal medicine. We found that most of BIA researches in TEAM were preliminary and remained in the peripheral levels which is far behind the western medical research activities. Therefore, more efforts are needed to study BIA in association with major subjects such as pattern identification or physiological/pathological phenomena. In addition, methodological breakthrough of BIA is possible by applying the diagnostic concepts of the TEAM in relation to the balance of Qi and Blood.

• The Journal of Korean Association of Computer Education
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• v.19 no.4
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• pp.45-58
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• 2016

This paper tried to analyze cases of one year team-based project of gifted students who spent two year programs in a math-IT integrated class as part of formative evaluation and extracted the factors associated with future enhancement for the program. The researcher as an advisory professor tried to guide the students as minimally as possible considering their levels of IT skills so that they could self-directedly perform the IT-fused project on a team basis. The data collection included documents, annual report, photos, video, artifacts and interview data with the students for the whole team project carried out between February and December, 2015. The overall pattern of the project activities has been stabilized in the middle of the course compared to the initial stages of brainstorming and design work even though the students revealed the differences of their programming skills and preferences toward the project theme. Their project outcomes were qualitatively analyzed according to the 9 steps of R&E model and has shown individual differences according to low, middle and high level. At the end, the analysis suggested several implications for further improvement of the mentorship program.

• Journal of Engineering Education Research
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• v.19 no.2
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• pp.34-44
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• 2016

This study was conducted to explore the applicability and effects of Smart Support System for Creative Problem Solving(S3CPS) in engineering education. S3CPS is an online system developed to support learners' creative problem solving activities based on the CPS model proposed by Treffinger et al.(2000). In this study, it was applied in an engineering course in which students work in teams to design and produce creative robots with the purpose of verifying its effects on creative problem solving and team projects. To address the purpose, interviews and surveys were carried out to examine the effects of S3CPS. The qualitative data were analyzed through a generic coding process. Three ways to apply S3CPS in an engineering course based on team project were derived and effects of the system were also analyzed. It implies that specific design guidelines are required for the optimal use of the system for existing engineering courses.