Browse > Article

Inhibitory Effect of Taraxci Herba Methanol Extract on Pro-inflammatory Mediator in Lipopolysaccharide;Activated Raw 264.7 cells  

Jo, Mi-Jeong (College of Oriental Medicine, BK21 Team, Daegu Haany University)
Chu, Yan-Hui (Department of Biochemistry, Key Laboratory of Heilongjiang Province, Mudanjiang Medical University)
Back, Young-Doo (College of Oriental Medicine, Daegu Haany University)
Lee, Byung-Wook (College of Oriental Medicine, Daegu Haany University)
Shin, Soon-Shik (College of Oriental Medicine, Dongeui University)
Kwon, Young-Kyu (College of Oriental Medicine, BK21 Team, Daegu Haany University)
Kim, Sang-Chan (College of Oriental Medicine, BK21 Team, Daegu Haany University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.4, 2008 , pp. 907-913 More about this Journal
Abstract
Taraxci Herba (TH; Pogongyoung in Korean) has been used in traditional oriental medicine for the treatment of various ailments. The biological activity of this plant is not yet evaluated systematically. This study was conducted to evaluate the inhibitory effects of TH on the production of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-activated Raw264.7 cells. The aim of the present work is to investigate a potential anti-inflammatory activity of TH. The Raw264.7 cells were cultured in DMEM medium for 24 h. After serum starvation for 12 h, the cells were treated with TH for 1 h, followed by stimulating NO production with LPS ($2{\mu}g/ml$). As result of this study, TH inhibited the levels of NO, PGE2, $TNF-{\alpha}$, IL-6 and $IL-1{\beta}$, and the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) activated by LPS. These inhibitory effects were mediated though the inhibition of phosphorylation of inhibitory kappa B ($I{\kappa}B$). These findings showed that TH could have some anti-inflammatory effects.
Keywords
Taraxci Herba; iNOS; COX-2;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Alderton, W.K., Cooper, C.E. and Knowles, R.G. Nitric oxide synthases: structure, function and inhibition. Biochem. J. 357: 593-615, 2001   DOI   ScienceOn
2 MacMicking, J., Xie, Q.W. and Nathan, C. Nitric oxide and macrophage fuction. Annu. Rev. Immunol. 15: 323-350, 1997   DOI   ScienceOn
3 Steinbach, G., Lynch, P.M., Phillips, R.K., Wallace, M.H., Hawk, E., Gordon, G.B., Wakabayashi, N., Saunders, B., Shen, Y., Fujimura, T., Su, L.K. and Levin, B. The effect of celecoxib, a cyclooxygenase-2 inhibitor, in familial adenomatous polyposis. Engl J Med. 342: 1946-1952, 2000   DOI   ScienceOn
4 Jean-Baptiste, E. Cellular mechanisms in sepsis. J Intensive Care Med. 22(2):63-72, 2007   DOI   ScienceOn
5 Kim, N.D., Kim, E.M., Kang, K.W., Cho, M.K., Choi, S.Y. and Kim, S.G. Ginsenoide Rg3 inhibits phenylephrine-induced vascular contraction though induction of nitric oxide synthase. Br. J. Pharmacol. 140: 661-670, 2003   DOI   ScienceOn
6 Yun, S.I., Cho, H.R. and Choi, H.S. Anticoagulant from Taraxacum platycarpum. Biosci Biotechnol Biochem. 66: 1859-1864, 2002   DOI   ScienceOn
7 Chen, F., Castranova, V. and Shi, X. New insight into the role of nuclear factor-kappaB in cell growth regulation. Am J Pathol. 159: 387-397, 2001   DOI   ScienceOn
8 Park, E.K., Shin, Y.W., Lee, H.U., Kim, S.S., Lee, Y.C., Lee, B.Y. and Kim, D.H. Inhibitory effect of ginsenoside Rb1 and compound K on NO and prostaglandin E2 biosyntheses of Raw264.7 cells induced by lipopolysaccharide. Biol. Pharm. Bull. 28(4):652-656, 2005   DOI   ScienceOn
9 Puskas, B.L., Menke, N.E., Huie, P., Song, Y., Ecklund, K., Trindade, M.C., Smith, R.L. and Goodman, S.B. Expression of nitric oxide, peroxynitrite, and apoptosis in loose total hip replacements. J. Biomed Mater Res. A. 66: 541-549, 2003
10 Shishodia, S. and Aggarwal, B.B. Nuclear factor-kappaB: a friend or a foe in cancer?. Biochem Pharmacol. 68: 1071-1080, 2004   DOI   ScienceOn
11 Lee, A.K., Sung, S.H., Kim, Y.C. and Kim, S.G. Inhibition of lipopolysaccharide- inducible nitric oxide synthase, TNF-alpha and COX-2 expression by sauchinone effects on I-kappaBalpha phosphorylation, C/EBP and AP-1 activation. Br J Pharmacol. 139: 11-20, 2003   DOI   ScienceOn
12 Miljkovic, D. and Trajkovic, V. Inducible nitric oxide synthase activation by interleukin-17. Cytokine Growth Factor Rev. 15: 21-32, 2004   DOI   ScienceOn
13 Bogdan, C. Nitric oxide and the immune response. Nat Immunol. 2(10):907-916, 2001   DOI   ScienceOn
14 Ravi, R. and Bedi, A. NF-kappa B in cancer-a friend turned foe. Drug Resist Updat. 7: 53-67, 2004   DOI   ScienceOn
15 Corriveau, C.C. and Danner, R.L. Endotoxin as a therapeutic target in septic shock. Infect Agents Dis. 2: 35-43, 1993
16 Mitchell, J.A., Belvisi, M.G., Akarasereenont, P., Robbins, R.A., Kwon, O.J., Croxtall, J., Barnes, P.J. and Vane, J.R. Induction of cyclooxygenase-2 by cytokines in human pulmonary epithelial cells: regulation by dexamethasone. Br. J. Pharmacol. 113: 1008-1014, 1994   DOI   ScienceOn
17 Castranova, V. Role of nitric oxide in the progression of pneumoconiosis. Biochemistry. 69: 32-37, 2004   DOI   ScienceOn
18 Watson, W.H., Zhao, Y. and Chawla, R.K. S-adenosylmethionine attenuates the lipopolysaccharide-induced expression of the gene for the tumour necrosis factor-a. Biochem J. 342: 21-25, 1999   DOI   ScienceOn
19 Kubes, P. and McCaferty, D.M. Nitric oxide and intestinal inflammation. Am. J. Med. 109: 150-158, 2000   DOI   ScienceOn
20 Palmer, R.M., Ashton, D.S. and Moncada, S. Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature. 333: 664-666, 1998   DOI   ScienceOn
21 Chun, S.C., Jee, S.Y., Lee, S.G., Park, S.J., Lee, J.R. and Kim, S.C. Anti-Inflammatory Activity of the Methanol Extract of Moutan Cortex in LPS-Activated Raw264.7 Cells. Evid Based Complement Alternat Med. 4(3):327-333, 2007   DOI   ScienceOn
22 Roshak, A.K., Jackson, J.R., Mcgough, K., Chabot-Fletcher, M., Mochan, E. and Marshall, L.A. Manipulation of distinct NF-kappaB proteins alters interleukin-1beta-induced human rheumatoid synovial fibroblast prostaglandin E2 formation. J Biol Chem. 271: 31496-31501, 1996   DOI   ScienceOn
23 Yoshimura, A. Signal transduction of inflammatory cytokines and tumor development. Cancer Sci. 97: 439-447, 2006   DOI   ScienceOn
24 Beutler, B. and Cerami, A. The biology of cachectin/TNF-$\alpha$ primary mediator of the host response. Annu Rev Immunol. 7: 625-655, 1989   DOI   ScienceOn
25 Takabayashi, T., Shimizu, S., Clark, B.D., Beinborn, M., Burke, J.F. and Gelfand, J.A. Interleukin-1 upregulates anaphylatoxin receptors on mononuclear cells. Surgery. 135(5):544-554, 2004   DOI   ScienceOn
26 West, M.A., Seatter, S.C., Bellingham, J. and Clair, L. Mechanisms of reprogrammed macrophage endotoxin signal transduction after lipopolysaccharide pretreatment. Surgery. 118(2):220-228, 1995   DOI   ScienceOn
27 Kim, Y.W., Zhao, R.J., Park, S.J., Lee, J.R., Cho, I.J., Yang, C.H., Kim, S.G. and Kim, S.C. Anti-inflammatory effects of liquiritigenin as a consequence of the inhibition of NF-kB-dependent iNOS and proinflammatory cytokines production. Br J Pharmacol. pp 165-173, 2008
28 Zhai, Z., Haney, D., Wu, L., Solco, A., Murphy, P.A., Wurtele, E.S., Kohut, M.L. and Cunnick, J.E. Alcohol extracts of Echinacea inhibit production of nitric oxide and tumor necrosis factor-alpha by macrophages in vitro. Food Agric Immunol. 18: 221-236, 2007   DOI   ScienceOn
29 Gruenwald, J., Brendler, T. PDR for Herbal Medicines (4th Edition). Thomson Healthcare. pp 252-254. 2004
30 Hedger, M.P. and Meinhardt, A. Cytokines and the immune-testicular axis. J Reprod Immunol. 58: 1-26, 2003   DOI   ScienceOn