• Journal of Microbiology and Biotechnology
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• 제31권9호
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• pp.1323-1329
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• 2021

Micro-scale magnetic beads are widely used for isolation of proteins, DNA, and cells, leading to the development of in vitro diagnostics. Efficient isolation of target biomolecules is one of the keys to developing a simple and rapid point-of-care diagnostic. A zinc finger protein (ZFP) is a double-stranded (ds) DNA-binding domain, providing a useful scaffold for direct reading of the sequence information. Here, we utilized two engineered ZFPs (Stx2-268 and SEB-435) to detect the Shiga toxin (stx2) gene and the staphylococcal enterotoxin B (seb) gene present in foodborne pathogens, Escherichia coli O157 and Staphylococcus aureus, respectively. Engineered ZFPs are immobilized on a paramagnetic bead as a detection platform to efficiently isolate the target dsDNA-ZFP bound complex. The small paramagnetic beads provide a high surface area to volume ratio, allowing more ZFPs to be immobilized on the beads, which leads to increased target DNA detection. The fluorescence signal was measured upon ZFP binding to fluorophore-labeled target dsDNA. In this study, our system provided a detection limit of ≤ 60 fmol and demonstrated high specificity with multiplexing capability, suggesting a potential for development into a simple and reliable diagnostic for detecting multiple pathogens without target amplification.

• 전자공학회논문지
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• 제53권2호
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• pp.133-140
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• 2016

해안 수중 감시를 위하여 분산센서망를 해안에 설치하고, 이를 이용하여 표적을 탐지하고 표적의 위치를 추정하는 연구가 많이 이루어지고 있다. Zhou 등은 표적 탐지만 가능한 간단한 구조의 센서들로 구성된 분산센서망에서 표적을 탐지한 센서들의 위치 정보를 활용하여 표적의 위치를 추정하는 기법을 제안하였다. Zhou 등이 제안한 기법은 다른 기존의 기법에 비해 표적탐지 신호의 전파모델에 대한 파라미터들을 별도로 추정할 필요가 없고, 연산량이 적으며, 분산센서망에서 적은 량의 데이터만 송수신하여도 된다. 그러나 Zhou 기법은 표적의 위치 추정오차가 크다. Ryu는 추정오차를 줄이기 위하여 Zhou 기법을 수정하였다. 수정된 Zhou 기법은 Zhou 기법보다 추정성능이 향상되었지만, 여전히 비교적 큰 추정오차를 가지고 있다. 본 논문에서는 수정된 Zhou 기법으로 구한 표적의 방위각을 나타내는 직선과 표적을 탐지한 센서들과의 기하학적 구조를 고려한 표적위치 추정기법을 제안하였으며, 수정된 Zhou 기법에 기반을 두고 있다. 제안한 기법의 표적위치 추정성능이 Zhou 기법과 수정된 Zhou 기법 보다 향상되었음을 컴퓨터 시뮬레이션을 통하여 확인하였다.

• 전자공학회논문지
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• 제49권9호
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• pp.362-369
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• 2012

최근에 능동소나 분야에서 분산센서망을 이용하여 표적을 탐지하는 연구가 많이 이루어지고 있다. Zhou 등은 표적의 탐지만 가능한 간단한 구조의 센서들로 구성된 분산센서망에서 라인피팅(line fitting)을 이용하여 표적의 위치를 추정하는 기법을 제안하였다. 이 기법은 ML(Maximum Likelihood) 기법에 비해 3가지 장점을 가지고 있다. 첫째는, 음파전달 모델에 대한 파라미터들을 추정할 필요가 없으며, 둘째는 연산량이 적다. 셋째는 분산센서망에서 센서들이 표적을 탐지했다는 정보만 이용하기 때문에 데이터처리 센터는 적은 량의 데이터만 수집하여도 된다. 그러나 이 기법은 표적의 위치 추정오차가 크다는 단점을 가지고 있다. 본 논문에서는 Zhou의 기법이 가지는 큰 위치 추정오차를 줄이기 위하여 Zhou가 제안한 표적위치 추정기법을 수정하였다. 본 논문에서 제안한 수정된 표적위치 추정기법은 Zhou의 기법보다 40.7%의 위치 추정오차가 감소하는 성능향상을 보였다.

• 전자공학회논문지SC
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• 제47권6호
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• pp.27-32
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• 2010

오늘날 FMCW 레이더 시스템은 차량의 능동 안전 시스템에 널리 활용되어지고 있다. 레이더 시스템에서 탐지확률을 높이고 오경보율을 줄이기 위해서는 정지타겟을 포함한 클러터 성분을 제거하고 관심 있는 이동타겟을 효과적으로 탐지하기 위한 알고리즘이 필요하다. 본 논문에서는 이동타겟 탐지 및 클러터 제거 알고리즘을 제안한다. 먼저 제안된 이동타겟 비트주파수 검출 필터를 이용하여 클러터를 제거한다. 검출된 이동타겟 비트주파수들을 이용하여 데이터-결합 한 후, 제로-도플러 제거 알고리즘을 이용하여 남아있는 클러터를 제거한다. 이 알고리즘을 검증하기 위해 실제 FMCW 레이더를 이용하여 도로 상에서 실험하였다.

• 대한원격탐사학회지
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• 제40권2호
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• pp.167-177
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• 2024

With advancements in satellite technology, interest in target detection and identification is increasing quantitatively and qualitatively. Synthetic Aperture Radar(SAR) images, which can be acquired regardless of weather conditions, have been applied to various areas combined with machine learning based detection algorithms. However, conventional studies primarily focused on the detection of stationary targets. In this study, we proposed a method to identify moving targets using an algorithm that integrates sub-aperture SAR images and cosine similarity calculations. Utilizing a transformer-based deep learning target detection model, we extracted the bounding box of each target, designated the area as a region of interest (ROI), estimated the similarity between sub-aperture SAR images, and determined movement based on a predefined similarity threshold. Through the proposed algorithm, the quantitative evaluation of target identification capability enhanced its accuracy compared to when training with the targets with two different classes. It signified the effectiveness of our approach in maintaining accuracy while reliably discerning whether a target is in motion.

• ETRI Journal
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• 제35권1호
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• pp.80-88
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• 2013

A passive infrared or pyroelectric infrared (PIR) sensor is mainly used to sense the existence of moving objects in an indoor environment. However, in an outdoor environment, there are often outbreaks of false alarms from environmental changes and other sources. Therefore, it is difficult to provide reliable detection outdoors. In this paper, two algorithms are proposed to reduce false alarms and provide trustworthy quality to surveillance systems. We gather PIR signals outdoors, analyze the collected data, and extract the target features defined as window energy and alarm duration. Using these features, we model target and false alarms, from which we propose two target decision algorithms: window energy detection and alarm duration detection. Simulation results using real PIR signals show the performance of the proposed algorithms.

• 선박안전
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• 통권38호
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• pp.69-77
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• 2015

If we don't know the intention of altering course of a target ship when being in a head-on or a crossing situation, we may be confused about our decision making to change our course for collision avoidance and be in a danger of collision. In order to solve these problems, we need to develop an automatic detection system on altering course of a target ship for efficient collision avoidance. In this paper, we proposed an early detection system on altering course of a target ship using the steering wheel signal. This system will contribute to the reduction of collision accidents and also be used to the VTS system and the analysis of marine accidents.

• 한국컴퓨터정보학회논문지
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• 제21권10호
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• pp.49-54
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• 2016

In this paper, the confirmation method of target detection for the vehicle mounted metal detector (MD) has been described. The vehicle mounted MD with the arrayed 6 coils to detect the width of 2.4 m was demonstrated. It is important and necessary to inform the location of the objects detected by the MD. The confirmation method of target detection was verified by using the MD GUI and the analysis of the receive signal processing. The receive signal processing is performed by comparing the threshold and the difference of the signal calibrated at initial location and the signal detected at present location.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1991년도 한국자동제어학술회의논문집(국내학술편); KOEX, Seoul; 22-24 Oct. 1991
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• pp.120-124
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• 1991

In this paper, we consider the maneuvering detection and target tracking problem in uncertain linear discrete-time systems. The maneuvering detection is based on X$^{2}$ test[2,71, where Kalman filters have been utilized so far. The target tracking is performed by the maneuvering input compensation based on a maximum likelihood estimator. KF has been known to diverge when some modelling errors exist and fail to detect the maneuvering and to track the target in uncertain systems. Thus this paper adopt the FIR filter[l], which is known to be robust to modelling errors, for maneuvering detection and target tracking problem. Various computer simulations show the superior performance of the FIR filter in this problem.

• 선박안전
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• 통권36호
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• pp.71-78
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• 2014

If we don't know the intention of altering course of a target ship when being in a head-on or a crossing situation, we may be confused about our decision making to change our course for collision avoidance and be in a danger of collision. In order to solve these problems, we need to develop an automatic detection system on altering course of a target ship for efficient collision avoidance. In this paper, we proposed an early detection system on altering course of a target ship using the steering wheel signal. This system will contribute to the reduction of collision accidents and also be used to the VTS system and the analysis of marine accidents.