• Title/Summary/Keyword: Tag Detection

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Rapid Detection Kit for Staphylococcus aureus (황색포도상구균 신속 검출용 간이 진단키트)

  • Kim, Gi-Young;Park, Saet-Byeol;Moon, Ji-Hea;Lee, Sang-Dae;Lee, Sea-Rom;Jang, Youn-Jung
    • Korean Journal of Agricultural Science
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    • v.40 no.2
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    • pp.139-146
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    • 2013
  • This study was performed to develop a rapid test kit for pathogenic Staphylococcus in various samples. The rapid detection kit has been fabricated based on nitrocellulose lateral-flow strip. Colloidal gold and Staphylococcus antibodies were used as a tag and a receptor, respectively. Manually spotted Staphylococcus antibody and anti-mouse antibody on the surface of nitrocellulose membrane were used as test and control lines, respectively. Feasibility of the rapid kit to detect Staphylococcus aureus in samples were evaluated. The intensity of the color of the test line started to increase with the samples in which higher concentration of the cells were contained. The sensitivity of the sensor was $10^6$ cfu/mL Staphylococcus spiked in PBS. Also, the rapid test kit could detect $10^5$ cfu/mL of Staphylococcus in chicken meat extract.

Detection of cryIB Genes in Bacillus thuringiensis subsp. entomocidus and subsp. subtoxicus

  • CHOI, SOO KEUN;BYUNG SIK SHIN;BON TAG KOO;SEUNG HWAN PARK;AND JEONG IL KIM
    • Journal of Microbiology and Biotechnology
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    • v.4 no.3
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    • pp.171-175
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    • 1994
  • To find new crystal protein genes, we screened 42 Bacillus thuringiensis strains of serovar standards by Southern hybridization with a cryI-specific probe which was amplified from B. thuringiensis subsp. kurstaki HDl by polymerase chain reaction (PCR). Two strains, B. thuringiensis subsp. entomocidus HD9 and subsp. subtoxicus HD109, generated weak signals under the low-stringency hybridization conditions. Further analysis with Southern hybridization revealed that the two strains contained cryIB genes which are slightly different from those of B. thuringiensis subsp. thuringiensis HD2. These results were confirmed by PCR with cryIB-specific primers followed by the restriction analysis of PCR products.

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Protein Array Fabricated by Microcontact Printing for Miniaturized Immunoassay

  • Lee Woo-Chang;Lim Sang-Soo;Choi Bum-Kyoo;Choi Jeong-Woo
    • Journal of Microbiology and Biotechnology
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    • v.16 no.8
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    • pp.1216-1221
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    • 2006
  • A protein array was fabricated for a miniaturized immunoassay using microcontact printing ($\mu$CP). A polydimethylsiloxane (PDMS) stamp with a 5 $\mu$m$\times$5 /$\mu$m dimension was molded from a silicon master developed by photolithography. Under optimal fabrication conditions, including the baking, incubation, and exposure time, a silicon master was successfully fabricated with a definite aspect ratio. An antibody fragment was utilized as the ink for the $\mu$CP, and transferred to an Au substrate because of the Au-thiol (-SH) interaction. The immobilization and antibody-antigen interaction were investigated with fluorescence microscopy. When human serum albumin (HSA) was applied to the protein array fabricated with an antibody against HSA, the detection limit was 100 pg/ml of HSA when using a secondary antibody labeled with a fluorescence tag. The fabricated protein array maintained its activity for 14 days.

Detection of Allexiviruses in the Garlic Plants in Korea

  • Lee, Eun-Tag;Koo, Bong-Jin;Jung, Ji-Hue;Chang, Moo-Ung;Kang, Sang-Gu
    • The Plant Pathology Journal
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    • v.23 no.4
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    • pp.266-271
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    • 2007
  • The genomes of different allexiviruses were isolated and cloned from virus-infected garlic plants (Allium sativum), which were collected from farm fields in the southern provinces in Korea. The partial nucleotide sequences of the genomes from different allexiviruses were clearly identified in the virus-infected garlic plants. The cloned partial genomes of viruses in garlic plants showed a greater than 90% homology to previously identified allexiviruses and classified into species of GarV-A, -B, -C, -D, -E, and -X, demonstrating that species of allexivirus found in the other countries in the world are also widely distributed in the garlic plants in Korea.

Detection and prevention of laboratory Risk with Arduino sensors (아두이노 센서를 통한 실험실 위험 감지 및 예방)

  • Park, Min-Sik;Hong, Seok-Hun;Jin, Hyun-Tag;Ryu, Guan Hee
    • Proceedings of the Korea Information Processing Society Conference
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    • 2018.05a
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    • pp.6-8
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    • 2018
  • 본 논문은 아두이노를 사용하여 실내에서 발생하는 안전사고에 대하여 사전에 위험을 감지하고 예방하는 시스템을 다루고 있다. 아두이노에 진동감지센서, 화재감지센서, 공기농도센서, 온습도감지센서를 연결하여 각 센서의 수치에 따라 알림을 모니터에 나타내어 지진, 화재, 질식 사고를 예방 힐 수 있다.

Expressed Sequence Tag Analysis of Toxic Alexandrium tamarense and Identification of Saxitoxin Biosynthetic Genes (독성 Alexandrium tamarense 의 EST 분석 및 삭시톡신 생합성 유전자의 확인)

  • Chang, Man;Lee, Juyun;Chung, Youngjae;Lee, Gunsup;Kim, Dongguin;Lee, Taek-Kyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.7
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    • pp.3582-3588
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    • 2013
  • Expressed sequence tag (EST) library was constructed from A. tamarense. Base sequences of EST clones were analyzed and saxitoxin biosynthesis-related genes were cloned. Sequences of 827 clones were analyzed and 564 EST were functionally clustered using Blast searches against GenBank. Main genes in the EST had functions on cellular organization, cell metabolism, energy, cell cycle and DNA processing, cellular transport and transport, cell rescue, defense, death and aging, and transcription. Moreover, expression of S-adenosylmethionine synthetase and H2A histone family genes were increased in the toxic A. tamarense. These results show that two genes could be a good biomarkers for the detection of saxitoxin biosynthesis in the A. tamarense.

Performance Assessment of High-Speed Transponder System for Rail Transport on High-Speed Line (철도교통용 고속 트랜스폰더시스템 고속선 실차 성능평가)

  • Park, Sungsoo;Lee, Jae-Ho;Kim, Seong Jin
    • Journal of the Korean Society for Railway
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    • v.19 no.3
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    • pp.304-313
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    • 2016
  • It is necessary to receive telegrams transmitted by transponder tags installed along the track in order to detect the exact position of a high-speed train. In a high-speed railway environment, telegrams can be corrupted by the electromagnetic interference that comes from onboard electric train power equipment or wayside devices. In this study, we verified the railway environment compatibility of a high-speed transponder system developed as a train position detection system. We installed transponder tags on the Honam high-speed line and measured the number of error-free telegrams received from the transponder tag while the HEMU-430X was running at 268km/h~334km/h. Based on the measurement, we estimated the length of the contact zone formed between the transponder reader and tag. Field test results allow us to estimate how many error-free telegrams can be received when HEMU-430X is at speeds up to 400km/h.

Mobile application for preventing card loss using the NFC technology (NFC 기술을 이용한 카드 분실을 방지하기 위한 모바일 앱)

  • Kang, Ki-Beom;Jwa, Jeong-Woo;Kim, Soon-Whan;Kim, Heung-Soo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.12 no.1
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    • pp.181-188
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    • 2017
  • As smart phone users increase the number of users who insert credit cards into wallet type smart phone cases is increasing. In this paper, we developed an Android application for preventing credit card loss in a wallet type smart phone case using the NFC (Near Field Communication) technology of smart phone. The developed android application uses the NFC reading and writing functions of the smart phone. When the credit card is inserted into the wallet type smart phone case, the android application detects the NFC tag in the credit card and registers credit card information. When the credit card is removed from the smart phone case, the Android application automatically detects the credit card loss and notifies to the user. The user can check and finds the location information of the lost credit card on the map of the Android application. We can easily develop the Android application for preventing credit card loss when a function that automatically detects an leaving NFC tag is added to Android.

A Development of the DIRCM Effectiveness Analysis Simulator based on DEVS (DEVS 기반 DIRCM 효과도 분석 시뮬레이터 개발)

  • Shin, Baek-Cheon;Hur, Jang-Wook;Kim, Tag-Gon;Kim, Mi-Jeong
    • Journal of the Korea Society for Simulation
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    • v.27 no.2
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    • pp.115-123
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    • 2018
  • we analyzed simulation of the effectiveness for one or two DIRCM on a helicopter. The survival rate of helicopter followed increase of the deception rate of DIRCM. When the deception rate was over 70% at 100% detection rate, the survival rate was 10~30% when one DIRCM was installed and the survival rate was 70~80% when two DIRCMs were installed. When the detection rate was over 70% at 100% deception rate the survival rate was 10~30% case of one DIRCM was installed. survival rate was 20~30% when two were installed. Survival rate of 70~90% was observed with one DIRCM when the deception rate and detection rate were 100%, and 100% with two DIRCMs.

Performance Analysis of Wireless Communications between Tag and Reader in EPCglobal Gen-2 RFID System (EPCglobal Gen-2 RFID 시스템 태그와 리더간의 무선 전송 방식 성능 분석)

  • Yoon, Hee-Seok;Mohaisen, Manar;Chang, Kyung-Hi;Bae, Ji-Hoon;Choi, Gil-Young
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.18 no.9
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    • pp.1047-1056
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    • 2007
  • In this paper, we analyze the performance of the encoding and the modulation processes in the downlink and uplink of the EPCglobal Gen-2 system through the analysis and simulation. Furthermore, the synchronization issues on time and frequency domain and the preamble architecture are evaluated. By incorporating the encoding and the modulation technigue in the downlink and uplink, we assess the performance of the EPCglobal Gen-2 system. We also introduce the encoding and backscatter modulation process as well as the BER performance of FM0 code. In addition, the importance and the role of the frequency and time synchronization, such as the preamble and frame synchronization are explained. Through the simulation in the uplink on the detection probability through preamble, we find that the detection probability approaches 1 for 13 dB $E_b/N_0$.