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Protein Array Fabricated by Microcontact Printing for Miniaturized Immunoassay  

Lee Woo-Chang (Interdisciplinary Program of Integrated Biotechnology, Sogang University)
Lim Sang-Soo (Department of Mechanical Engineering, Sogang University)
Choi Bum-Kyoo (Department of Mechanical Engineering, Sogang University)
Choi Jeong-Woo (Interdisciplinary Program of Integrated Biotechnology, Sogang University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.8, 2006 , pp. 1216-1221 More about this Journal
Abstract
A protein array was fabricated for a miniaturized immunoassay using microcontact printing ($\mu$CP). A polydimethylsiloxane (PDMS) stamp with a 5 $\mu$m$\times$5 /$\mu$m dimension was molded from a silicon master developed by photolithography. Under optimal fabrication conditions, including the baking, incubation, and exposure time, a silicon master was successfully fabricated with a definite aspect ratio. An antibody fragment was utilized as the ink for the $\mu$CP, and transferred to an Au substrate because of the Au-thiol (-SH) interaction. The immobilization and antibody-antigen interaction were investigated with fluorescence microscopy. When human serum albumin (HSA) was applied to the protein array fabricated with an antibody against HSA, the detection limit was 100 pg/ml of HSA when using a secondary antibody labeled with a fluorescence tag. The fabricated protein array maintained its activity for 14 days.
Keywords
Microcontact printing; poly(dimethylsiloxane); protein array; fluorescence microscopy; sandwich-type immunoassay;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
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