• Title/Summary/Keyword: TRAP1

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Comparison of Occurrences of Coleoptera by Three Sampling Methods in Mt. Yeonyeop Area, Korea (채집법에 따른 연엽산 일대 딱정벌레목의 출현상 비교 분석)

  • Jeong Jong-Kook;Lee Seung-Il;Choi Jae-Seok;Kwon Oh-Kil
    • Korean Journal of Environmental Biology
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    • v.23 no.3 s.59
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    • pp.228-237
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    • 2005
  • To compare the occurrence of Coleoptera by different sampling methods such as light trap, pitfall trap and sweeping, we collected samples every month from April to September,2004 in the Mt. Yeonyeop, Gangwon-do, Korea. According to the sampling methods, the species composition, abundance and dry weight were completely different. We collected 151 species in 35 families (690 individuals) by sweeping method, 148 species in 30 families (689 individuals) by light trap, and 112 species in 18 families (1,674 individuals) by pitfall trap, respectively. The dry weight in collected sample was about 181.46 g in pitfall trap,39.85 g in light trap, and 10.89 g in sweeping method, respectively. Relatively high flight and small-sized beetles such as Coccinellidae, Nitidulidae, Scarabaeidae were collected in light trap. The species diversity was high in July. Unlike the samples collected in light trap, the pitfall trap samples were big-sized saprophagous or carnivorous beetles such as Carabidae, Silphidae, Staphylinidae. The pitfall trap showed relatively the higher number of individual and lower species diversity compared to other methods. The major samples collected by sweeping method were small-sized carnivorous or herbivorous beetles such as Chrysomelidae, Curculionidae, Coccinellidae. The peak of species diversity occurred in May. The similarity was calculated with the Jaccard's index over the light trap-pitfall trap was 0.07, light trap-sweeping was 0.10, and pitfall trap-sweeping was 0.01. Consequently, similarity of sampling methods was relatively low. In conclusion, efficiency of the each sampling methods significantly differed in the species composition of Coleoptera. This study emphasize the necessity of using three sampling methods in the area of diversity research.

TRAP1 regulation of mitochondrial life or death decision in cancer cells and mitochondria-targeted TRAP1 inhibitors

  • Kang, Byoung-Heon
    • BMB Reports
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    • v.45 no.1
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    • pp.1-6
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    • 2012
  • Hsp90 is one of the most conserved molecular chaperones ubiquitously expressed in normal cells and over-expressed in cancer cells. A pool of Hsp90 was found in cancer mitochondria and the expression of the mitochondrial Hsp90 homolog, TRAP1, was also elevated in many cancers. The mitochondrial pool of chaperones plays important roles in regulating mitochondrial integrity, protecting against oxidative stress, and inhibiting cell death. Pharmacological inactivation of the chaperones induced mitochondrial dysfunction and concomitant cell death selectively in cancer cells, suggesting they can be target proteins for the development of cancer therapeutics. Several drug candidates targeting TRAP1 and Hsp90 in the mitochondria have been developed and have shown strong cytotoxic activity in many cancers, but not in normal cells in vitro and in vivo. In this review, recent developments in the study of mitochondrial chaperones and the mitochondria-targeted chaperone inhibitors are discussed.

Mosquito Distribution and Detection of Flavivirus Using Real Time RT-PCR in Jeju Island, 2017 (제주지역 모기의 계절적 발생소장 및 Real Time RT-PCR을 이용한 Flavivirus 감염조사(2017))

  • Lee, Che-Wook;Hwang, Kyu-Kye
    • Korean journal of applied entomology
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    • v.57 no.3
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    • pp.177-183
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    • 2018
  • This study examined the seasonal, regional distribution of mosquito vectors related with disease vectors in the Jeju. From March to November, sample were collected from 11 points in four environmentally different sites in Jeju Island. Samples were collected twice a month using a black-light trap and a BG sentinel trap. Overall, five genera and seven species types of 6,042 female mosquitos were collected. Among the collected mosquitos, 4,159 (68.8%) and 1,348 (24.4%) were Culex pipiens and Aedes albopictus, respectively, making them the dominant species. Additionally, collection using the black light trap produced 72.8 entities per trap in Jungang-dong service center in the center of the city, which was the highest value, while the lowest amount of 1.4 per trap was recovered from the airport. When the BG sentinel trap was used, the largest recovery was observed in the port, where there were 71.7 entities per trap, while the lowest amount of 28.3 entities per trap was recovered at Gealmae Eco Park. The overall number of mosquitoes collected started to increase from May, and reached the largest value of 1,156 (19.1%) in August. Trapped mosquitoes are created 364 pools of up to 50 grains per pool, by season, by environmental, and by species. When the pools were used, no flaviviral infection was observed upon real time RT-PCR.

Biodiversity of Invertebrate on Organic and Conventional Pear Orchards (유기와 관행재배 배 과수원의 무척추동물의 종 다양성 연구)

  • Kim, Do-Ik;Kim, Seon-Gon;Ko, Sug-Ju;Kang, Beom-Ryong;Choi, Duck-Soo;Lim, Gyeong-Ho;Kim, Sang-Soo
    • Korean Journal of Organic Agriculture
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    • v.19 no.1
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    • pp.93-107
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    • 2011
  • This research was carried out to investigate invertebrate fauna with organic and conventional pear orchards, which used four collected methods; soil sampling for soil microorganism, pitfall, malaise, and black light trap for over ground species. Collected species were 37 species, 1,184 individuals in organic and 28 species, 501 individuals by soil sampling in conventional pear fields. Those were 38 species, 646 individuals and 29 species, 440 individuals by pitfall trap, 55 species 650 individuals and 47 species, 508 individuals by malaise trap, and 23 species, 201 individuals and 9 species, 42 individuals by black light trap. Collembola was collected 389 individuals in organic which was 5 times than in conventional in soil sampling. In pitfall trap, that was 183 individuals which was 3 times. The diversity indices of organic pear orchards were 1.956 in May, 2.638 in August and those of conventional was 1.426 in May, 2.011 in August in soil sampling. In pitfall trap, the dominant species were spiders, collembollan, and coleopteran. Among Coleoptera, indicator insects for the evaluation of agricultural environment suggested were Eusilpha jakowelewi as organic pear orchard and Anisodactykus punctatipennis and Pheropsophus jessoensis as conventional. Malaise trap was collected dominant species as Diptera and Hymenoptera of Braconidae and Ichneumonidae. The diversity indices of organic pear orchards were 2.952, 3.120, and 2.010 in pitfall, malaise and black light trap in over ground invertebrate sampling. The highest diversity was in malaise trap. The higher diversity indices, the lower dominance indices.

A study on the Trap Density of Silicon Oxide (실리콘 산화막의 트랩 밀도에 관한 연구)

  • 김동진;강창수
    • Journal of the Korean Institute of Telematics and Electronics T
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    • v.36T no.1
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    • pp.13-18
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    • 1999
  • The trap density by the stress bias in silicon oxides with different thicknesses has been investigated. The trap density by stress bias was shown to be composed of on time current and off time current. The on time trap density was composed of dc current. The off time trap density was caused by the tunneling charging and discharging of the trap in the interfaces. The on time trap density was used to estimate to the limitations on oxide thicknesses. The off time trap density was used to estimate the data retention in nonvolatile memory devices.

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Simple Self Trap Cropping System to Control Tukra Mealy Bug (Maconellicoccus hirsutus Green) Incidence on Mulberry (Morus spp.)

  • Latha, K. Lavanya;Rao, T.V.S.S.;Rao, J. V. Krishna;Jayaraj, S.;Reddy, N. Sivarami;Naik, S. Sankar
    • International Journal of Industrial Entomology and Biomaterials
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    • v.17 no.2
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    • pp.201-203
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    • 2008
  • A field study on self trap cropping system in controlling tukra mealy bug Maconellicoccus hirsutus Green) incidence on mulberry (Morus spp.; V-1 variety; $3'{\times}3'$ spacing) gardens was undertaken in selected sericulture farmers' mulberry gardens of Pydeti village (Parigi Mandal, Anantapur District, Andhra Pradesh, India). The study was conducted for three years (2004, 2005 and 2006). Four treatments (T0; control/no treatment, T1; self trap cropping rows with 10 row interval, T2; self trap cropping rows with 10 row interval and treating these rows only with 0.5% neem oil emulsion and T3; self trap cropping rows with 10 rows intervals and treating all the mulberry rows including self trap cropping rows with 0.5% neem oil emulsion) were considered. The results indicated that the incidence increased in T0 and T1 while the same suppressed in T2 and T3. The suppression (%) of tukra incidence between T2 and T3 was not significant. Hence, T2 only was recommended to farmers as its economical viable practice. The results are discussed based on the importance of tukra, its suppression and cost of treatment.

Molecular Cloning of Differentially Expressed Genes in First Trap Leaf of Dionaea muscipula by Fluorescent Differential Display (형광 Differential Display법에 의한 파리지옥풀 포충잎트랩 특이발현 유전자 탐색)

  • Kang, Kwon-Kyoo;Lee, Keun-Hyang;Park, Jin-Heui;Hong, Kyong-Ei
    • Journal of Plant Biotechnology
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    • v.30 no.4
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    • pp.307-313
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    • 2003
  • Fluorescent differential display (FDD) is a method for identifying differentially expressed genes in eukaryotic cells. The mRNA FDD technology works by systematic amplification of the 3' terminal regions of mRNAs. This method involve the reverse transcription using anchored primers designed to bind 5'boundary of the poly A tails, followed by polymerase chain reaction (PCR) amplification with additional upstream primers of arbitrary sequences. The amplified cDNA subpopulations are separated by denaturing polyacrylamide electrophoresis. To identify the genes involved in the development of first trap leaf, we applied a FDD method using mRNAs from leaf base, first trap leaf and flower tissue, respectively. We screened several genes that expressed specifically in first trap leaf. Nucleotide sequence analysis of these genes revealed that these were protease inhibitor (PI), myo-inositol-1-phosphate synthase and lipocalin-type prostaglandin D synthase. Northern blot analysis showed that these genes were expressed specifically in first trap leaf (in vivo and in vitro). FDD could prove to be useful for simultaneous scanning of transcripts from multiple cDNA samples and faster selection of differentially expressed transcripts of interest.

Development of Rope Winding Device for Safety Fishing Operation of Small Trap Fishing Vessel (소형 통발어선의 안전조업을 위한 로프 권양장치 연구)

  • Kim, Dae-Jin;Jang, Duck-Jong;Park, Ju-Sam
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.28 no.1
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    • pp.19-29
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    • 2022
  • The result of a questionnaire survey conducted on fishermen using coastal fish traps shows that fall accidents during trap dropping and pulling constitute the highest proportion of accidents at 42.1 %, whereas slipping accidents on the deck or stricture accidents to the body due to the trap winding device constitute 21.1 % each. In addition, 53.2 % of all surveyed subjects responded that trap pulling is the most dangerous task, followed by fish sorting 33.8 %, and trap dropping 9.1 %. As for the main items requested by fishermen for improving the trap winding device, 36.8 % indicated a method to easily lift the trap from the water to the work deck, and 31.6 % indicated a method to overcome the rope tension and prevent slip when pulling the trap to reduce the accidents. The small trap fishing vessel winding device proposed herein can increase the winding force by strengthening the rope contact area and friction coefficient via an appropriate contact angle between the driving roller of the winding device and the rope. When the contact angles between the driving roller and the rope are 1°, 5°, 9°, 14° and 19°, the rope tension showed a difference according to each contact angle. When the contact angle is 9°, the rope tension is the highest at 392.62 kgf. Based on these experimental results, a prototype winding device is manufactured, and 25 traps are installed on a rope with a total length of 100 m at 4 m intervals in the sea, and then the rope tension is measured during trap pulling. As a result, the rope tension increases rapidly at the initial stage of trap pulling and shows the highest value of 31.89 kgf, which subsequently decreases significantly. Therefore, it is appropriate to design the winding force of a small trap fishing vessel winding device based on the maximum tension value of the rope specified at the beginning of the trap pulling operation.

Effect of NCF Trap on Electromigration Characteristics of Cu/Ni/Sn-Ag Microbumps (NCF Trap이 Cu/Ni/Sn-Ag 미세범프의 Electromigration 특성에 미치는 영향 분석)

  • Ryu, Hyodong;Lee, Byeong-Rok;Kim, Jun-beom;Park, Young-Bae
    • Journal of the Microelectronics and Packaging Society
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    • v.25 no.4
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    • pp.83-88
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    • 2018
  • The electromigration (EM) tests were performed at $150^{\circ}C$ with $1.5{\times}10^5A/cm^2$ conditions in order to investigate the effect of non-conductive film (NCF) trap on the electrical reliability of Cu/Ni/Sn-Ag microbumps. The EM failure time of Cu/Ni/Sn-Ag microbump with NCF trap was around 8 times shorter than Cu/Ni/Sn-Ag microbump without NCF trap. From systematic analysis on the electrical resistance and failed interfaces, the trapped NCF-induced voids at the Sn-Ag/Ni-Sn intermetallic compound interface lead to faster EM void growth and earlier open failure.

Trap characteristics of charge trap type NVSM with reoxidized nitrided oxide gate dielectrics (재산화 질화산화 게이트 유전막을 갖는 전하트랩형 비휘발성 기억소자의 트랩특성)

  • 홍순혁;서광열
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.12 no.6
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    • pp.304-310
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    • 2002
  • Novel charge trap type memory devices with reoxidized oxynitride gate dielectrics made by NO annealing and reoxidation process of initial oxide on substrate have been fabricated using 0.35 $\mu \textrm{m}$ retrograde twin well CMOS process. The feasibility for application as NVSM memory device and characteristics of traps have been investigated. For the fabrication of gate dielectric, initial oxide layer was grown by wet oxidation at $800^{\circ}C$ and it was reoxidized by wet oxidation at $800^{\circ}C$ after NO annealing to form the nitride layer for charge trap region for 30 minutes at $850^{\circ}C$. The programming conditions are possible in 11 V, 500 $\mu \textrm{s}$ for program and -13 V, 1ms for erase operation. The maximum memory window is 2.28 V. The retention is over 20 years in program state and about 28 hours in erase state, and the endurance is over $3 \times 10^3$P/E cycles. The lateral distributions of interface trap density and memory trap density have been determined by the single junction charge pumping technique. The maximum interface trap density and memory trap density are $4.5 \times 10^{10} \textrm{cm}^2$ and $3.7\times 10^{18}/\textrm{cm}^3$ respectively. After $10^3$ P/E cycles, interlace trap density increases to $2.3\times 10^{12} \textrm{cm}^2$ but memory charges decreases.