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http://dx.doi.org/10.5656/KSAE.2018.07.0.028

Mosquito Distribution and Detection of Flavivirus Using Real Time RT-PCR in Jeju Island, 2017  

Lee, Che-Wook (Laboratory of Veterinary Preventive Medicine, College of Veterinary Medicine, Jeju National University)
Hwang, Kyu-Kye (Laboratory of Veterinary Preventive Medicine, College of Veterinary Medicine, Jeju National University)
Publication Information
Korean journal of applied entomology / v.57, no.3, 2018 , pp. 177-183 More about this Journal
Abstract
This study examined the seasonal, regional distribution of mosquito vectors related with disease vectors in the Jeju. From March to November, sample were collected from 11 points in four environmentally different sites in Jeju Island. Samples were collected twice a month using a black-light trap and a BG sentinel trap. Overall, five genera and seven species types of 6,042 female mosquitos were collected. Among the collected mosquitos, 4,159 (68.8%) and 1,348 (24.4%) were Culex pipiens and Aedes albopictus, respectively, making them the dominant species. Additionally, collection using the black light trap produced 72.8 entities per trap in Jungang-dong service center in the center of the city, which was the highest value, while the lowest amount of 1.4 per trap was recovered from the airport. When the BG sentinel trap was used, the largest recovery was observed in the port, where there were 71.7 entities per trap, while the lowest amount of 28.3 entities per trap was recovered at Gealmae Eco Park. The overall number of mosquitoes collected started to increase from May, and reached the largest value of 1,156 (19.1%) in August. Trapped mosquitoes are created 364 pools of up to 50 grains per pool, by season, by environmental, and by species. When the pools were used, no flaviviral infection was observed upon real time RT-PCR.
Keywords
Mosquito; Jeju Island; Culex pipiens; Aedes albopictus; Flavivirus;
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1 Kim, C.Y., Oh, H., Song, J., Hur, M., Suh, J.H., Jheong, W.H,, Kim, J.T., Oh, H.S., Park, J.H., 2016. First detection of West Nile Virus in domestic pigeon in Korea. J. Vet. Sci. 17, 587-589.   DOI
2 Kim, H., Cha, G.W., Jeong, Y.E., Lee, W.G., Chang, K.S., Roh, J.Y., Yang, S.C., Park, M.Y., Park, C., Shin, E.H., 2015. Detection of Japanese encephalitis virus genotype V in Culex orientalis and Culex pipiens (Diptera: Culicidae) in Korea. PLoS. One. 10, doi: 10.1371/journal.pone.0116547.   DOI
3 Kim, H.C., Klein, T.A., Takhampunya, R., Evans, B.P., Mingmongkolchai, S., Kengluecha, A., Grieco, J., Masuoka, P., Kim, M.S., Chong, S.T., Lee, J.K., Lee, W.J., 2011. Japanese encephalitis virus in culicine mosquitoes (Diptera: Culicidae) collected at Daeseongdong, a village in the demilitarized zone of the Republic of Korea. J. Med. Entomol. 48, 1250-1256.   DOI
4 Kuno, G., Chang, G.J., Tsuchiya, K.R., Karabatsos, N., Cropp, C.B., 1998. Phylogeny of the genus Flavivirus. J. Virol. 72, 73-83.
5 Kutsuna, S., Kato, Y., Moi, M.L., Kotaki, A., Ota, M., Shinohara, K., Kobayashi, T., Yamamoto, K., Fujiya, Y., Mawatari, M., Sato, T., Kunimatsu, J., Takeshita, N., Hayakawa, K., Kanagawa, S., Takasaki, T., Ohmagari, N., 2015. Autochthonous dengue fever, Tokyo, Japan, 2014. Emerg. Infect. Dis. 21, 517-520.   DOI
6 Lee, D.K., 2017. Ecological characteristics and current status of infectious disease vectors in South Korea. J. Korean Med. Assoc. 60, 458-467.   DOI
7 Lee, S.H., Nam, K.W., Jeong, J.Y., Yoo, S.J., Koh, Y.S., Lee, S., Heo, S.T., Seong, S.Y., Lee, K.H., 2013. The effects of climate change and globalization on mosquito vectors: evidence from Jeju Island, South Korea on the potential for Asian tiger mosquito (Aedes albopictus) influxes and survival from Vietnam rather than Japan. PLoS. One. 8, e68512.   DOI
8 Murdock, C.C., Paaijmans, K.P., Cox-Foster, D., Read, A.F., Thomas, M.B., 2012. Rethinking vector immunology: the role of environmental temperature in shaping resistance. Nat. Rev. Microbiol. 10, 869-876.   DOI
9 Ravanini, P., Huhtamo, E., Ilaria, V., Crobu, M.G., Nicosia, A.M., Servino, L., Rivasi, F., Allegrini, S., Miglio, U., Magri, A., Minisini, R., Vapalahti, O., Boldorini, R., 2012. Japanese encephalitis virus RNA detected in Culex pipiens mosquitoes in Italy. Euro. Surveill. 17. pii: 20221.
10 Otero, M., Solari, H.G., Schweigmann, N., 2006. A stochastic population dynamics model for Aedes aegypti: formulation and application to a city with temperate climate. Bull. Math. Biol. 68, 1945-1974.   DOI
11 Ree, H.I., 2003. Taxonomic review and revised keys of the Korean mosquitoes (Diptera: Culicidae). Korean J. Entomol. 33, 39-52.   DOI
12 Reiter, P., 2001. Climate change and mosquito-borne disease. Environ. Health Perspect. 109, 141-161.
13 Schmidt, J.R., Elmansoury, H.K., 1963. Natural and experimental infection of Egyptian equines with West Nile virus. Ann. Trop. Med. Parasitol. 57, 415-427.   DOI
14 Yang, C.F., Chen, C.F., Su, C.L., Teng, H.J., Lu, L.C., Lin, C., Wang, C.Y., Shu, P.Y., Huang, J.H., Wu, H.S., 2010. Screening of mosquitoes using SYBR Green I-based real-time RT-PCR with group-specific primers for detection of Flaviviruses and Alphaviruses in Taiwan. J. Virol. Methods 168, 147-151.   DOI
15 Kim, H.C., Wilkerson, R.C., Pecor, J.E., Lee, W.J., Lee, J.S., O'Guinn, M.L., Klein, T.A., 2005. New records and reference collection of mosquitoes (Diptera: Culicidae) on Jeju Island, Republic of Korea, Entomol. Res. 35, 55-66   DOI