• BMB Reports
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• v.37 no.3
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• pp.298-303
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• 2004

In general, a SYPRO Ruby dye is well known as a sensitive fluorescence-based method for detecting proteins by one-or two-dimensional SDS-PAGE (1-DE or 2-DE). Based on the SYPRO Ruby dye system, the combined two-dimensional fibrin zymography (2-D FZ) with SYPRO Ruby staining was newly developed to identify the Bacillus sp. proteases. Namely, complex protein mixtures from Bacillus sp. DJ-4, which were screened from Doen-Jang (Korean traditional fermented food), showed activity on the zymogram gel. The gel spots on the SYPRO Ruby gel, which corresponded to the active spots showing on the 2-D FZ gel, were analyzed by a matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometric analysis. Five intracellular fibrinolytic enzymes of Bacillus sp. DJ-4 were detected through 2-D FZ. The gel spots on the SYPRO Ruby dye stained 2-D gel corresponding to 2-D FZ were then analyzed by MALID TOF MS. Three of the five gel spots proved to be quite similar to the ATP-dependent protease, extracellular neutral metalloprotease, and protease of Bacillus subtilis. Also, the extracellular proteases of Bacillus sp. DJ-4 employing this combined system were identified on three gels (e.g., casein, fibrin, and gelatin) and the proteolytic maps were established. This combined system of 2-D zymography and SYPRO Ruby dye should be useful for searching the specific protease from complex protein mixtures of many other sources (e.g., yeast and cancer cell lines).

• Journal of Microbiology and Biotechnology
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• v.16 no.5
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• pp.804-807
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• 2006

Bacterial serine proteases, especially those from Bacillus, have been extensively studied. Intracellular serine protease 1 (Isp1) is responsible for most of the proteolytic activity in B. subtilis. To identify Isp1 substrates and study its physiological functions, a mutant of Isp1, which has lost the enzymatic activity, was constructed. Through a GST affinity chromatographic method, several Bacillus proteins that specifically interacted with S246A mutant Isp1 protein were isolated and then identified by MALDI-TOF analysis. ClpC and elongation factor Tu (EF-Tu) were among those proteins specifically bound to mutant Isp1. In addition, several proteins involved in stationary phase adaptive response (such as RNA polymerase sigma factor, spoIIIE) were also identified. These findings led us to suggest that the major function of this serine protease, whose expression is greatly increased during the stationary phase, is to mediate transition of the cell into the stationary phase in a proper and timely manner.

• Journal of the Korean Wood Science and Technology
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• v.45 no.6
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• pp.772-786
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• 2017

This study investigated the characteristics of wood tar, produced as a byproduct during the production of charcoal using oak wood by the modified traditional kiln and mechanical steel kiln. The wood tar was analyzed with a number of techniques, including Py-GC/MS, NMR, MALDI-TOF, FT-IR, TG and DSC. The Py-GC/MS analysis indicated that modified traditional kiln generated a higher hydrocarbon ratio in the wood tar than that of mechanical steel kiln. On the other hand, mechanical steel kiln resulted in a higher proportion of phenolic and aromatic hydrocarbon components than that of modified traditional kiln. Those results were also confirmed by NMR analysis. The MALDI-TOF analysis suggested that the wood tar produced in the mechanical steel kiln had a slightly higher molecular weight than the wood tar produced in the modified traditional kiln. In addition, the FT-IR analysis showed characteristic peak of symmetrical stretching vibration of $CH_3$ from the modified traditional kiln while characteristic peaks of the C-C and C-O stretching vibration were observed from the mechanical steel kiln. Moreover, TG and DSC analysis suggested that the mechanical steel kiln is more thermally stable than that of modified traditional kiln. Those findings clearly showed that the method of making charcoal greatly affects the properties of wood tar.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.7
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• pp.4161-4165
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• 2013

Objective: To investigate discriminating protein patterns and potential biomarkers in serum samples between pre/postoperative pancreatic cancer patients and healthy controls. Methods: 23 serum samples from PC patients (12 preoperative and 11 postoperative) and 76 from healthy controls were analyzed using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique combined with magnetic beads-based weak cation-exchange chromatography (MB-WCX). ClinProTools software selected several markers that made a distinction between pancreatic cancer patients and healthy controls. Results: 49 m/z distinctive peaks were found among the three groups, of which 33 significant peaks with a P < 0.001 were detected. Two proteins could distinguish the preoperative pancreatic cancer patients from the healthy controls. About 15 proteins may be potential biomarkers in assessment of pancreatic cancer resection. Conclusion: MB-MALDI-TOF-MS method could generate serum peptidome profiles of pancreatic cancer and provide a new approach to identify potential biomarkers for diagnosis and prognosis of this malignancy.

• Journal of Korea Multimedia Society
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• v.15 no.8
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• pp.1067-1074
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• 2012

In this paper, we proposed a contents of Chinese characters learning based on gesture recognition using HMM(hidden markov model) algorithm. Input image of the system is obtained in 3-dimensional information from the TOF camera, and the method of gesture recognition is consisted of part of forecasting user's posture in two infrared images and part of recognizing gestures from continuous poses. In the communication between human and computer, this system provided convenience that user can manipulate it easily by not using any further equipment but action. Because this system raise immersion and interest by using two large display and various multimedia factor, it can maximize information transmission. The edutainment Chinese character contents proposed in this paper provide educational effect that use can master Chinese character naturally with interest, and it can be expected a synergy effect via content experience because it is based on gesture recognition.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.7
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• pp.1477-1486
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• 2012

In this paper, we propose Multi-view CGH Making System using method of generation of virtual view-point depth image. We acquire reliable depth image using TOF depth camera. We extract parameters of reference-view cameras. Once the position of camera of virtual view-point is defined, select optimal reference-view cameras considering position of it and distance between it and virtual view-point camera. Setting a reference-view camera whose position is reverse of primary reference-view camera as sub reference-view, we generate depth image of virtual view-point. And we compensate occlusion boundaries of virtual view-point depth image using depth image of sub reference-view. In this step, remaining hole boundaries are compensated with minimum values of neighborhood. And then, we generate final depth image of virtual view-point. Finally, using result of depth image from these steps, we generate CGH. The experimental results show that the proposed algorithm performs much better than conventional algorithms.

• Journal of the Korean Society for Nondestructive Testing
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• v.36 no.6
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• pp.474-482
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• 2016

A Kelvin foam plate - widely used in the energy and transport industries as a lightweight structural material - was examined to estimate its Young's modulus using ultrasound. An isotropic tetrakaidecahedron foam structure was designed in SolidWorks and printed using 3D printer with an ABS plastic material. The 3D printed foam structure was used to build a foam plate with a 14 mm thickness ($50mm{\times}100mm$ in size) for the ultrasonic test. The Kelvin foam plate, a significantly porous medium, was completely filled with paraffin wax to enable the ultrasound to penetrate through the porous medium. The acoustic wave velocity of the wax-filled Kelvin foam was measured using the time of flight (TOF) method. Furthermore, the elastic modulus of the Kelvin foam was estimated based on an elastic structural model developed in this study. The Young's modulus of the produced Kelvin foam was observed to be approximately 3.4% of the bulk value of the constituent material (ABS plastic). This finding is consistent with experimental and theoretical results reported by previous studies.

• Journal of the Korean Society of Radiology
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• v.1 no.1
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• pp.45-49
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• 2007

keV-neutron capture gamma-ray spectrum of $^{197}Au$(natural gold) sample have been measured in neutron energy range from 10 to 90 keV using the 3-MV pelletron accelerator of the Research Laboratory for Nuclear Reactors at the Tokyo Institute of Technology. Pulsed keV neutrons were produced from the $^7Li(p,n)^7Be$ reaction by bombarding on the $^7Li$ target with the 1.5-ns bunched proton beam. The incident neutron spectrum on the Au sample was measured by a $^6Li$-glass scintillation detector and TOF method. Capture gamma-rays from Au sample were measured by anti-Compton NaI(TI) spectrometer. Five average neutron energy regions were selected to obtain the neutron capture spectrum. Several gamma-ray peaks in the spectrum were found in the present experiment.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.12
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• pp.2792-2799
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• 2012

Multilateration that consists of three or more fixed nodes has been widely used in the field of indoor real time location system(RTLS). However, when one or two among fixed nodes are partially out of communication reachability due to obstruction and unstable node, it is difficult to obtain an efficient location information. In order to improve the challenges of poor ranging measurements and fluctuations in these environment, this paper presents, based on TOF(Time of Flight), a new algorithm which can reduce the inherent ranging measurements errors in the conventional multilateration using a vector prediction algorithm for the displacement estimation of mobile node and Kalman filter for an efficient distance average. Even if a person moves with mobile node and then it fails ranging measurement from some of fixed nodes, the proposed algorithm using a present and previous measurement value can predict the distance between mobile node and fixed node which fails ranging measurement. The experimental results are shown that the proposed system improves the localization accuracy and efficiency compared with conventional method, respectively.

• Journal of Embryo Transfer
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• v.25 no.4
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• pp.281-286
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• 2010

This study was to evaluate the protein profile of seminal plasma using 2-DE in Hanwoo. Seminal plasma was harvested from five mature Hanwoo, and seminal plasma protein was extracted by M-PER Mammalian Protein Extraction Reagent. Proteins were refined by clean-up kit and quantified by Bradford method until total protein was $300\;{\mu}l$. Immobilized pH gradient (IPG) strip was used 18 cm and 3~11 NL. SDS-PAGE was used 12% acrylamide gel. Each gels were visualized by comassie brilliant blue and silver staining. These spots were analyzed by MALDI-TOF MS and searched on NCBInr. The result, 20 proteins of 36 protein spots were searched through peptide sequencing on the NCBInr. 8 proteins profiled by 2-DE were proved through previous bovine studies and the name of each protein was albumin, nucleobindin, clusterin, TIMP-2, spermadhesin Z13, spermadhesin-1 and BSP proteins (BSP 30 kDa and BSP A1/A2). 12 new proteins were ATP synthase, protein MAK16 homolog, Transmembrane protein 214, E3 ubiquitin-protein ligase BRE1A, dual serine/threonine and tyrosine protein kinase, tissue factor pathway inhibitor 2, alpha-actinin-4, RUN domain-containing protein 3B, catenin alpha-1, protein-glutamine gamma-glutamyltransferase 2, plakophilin-1 and inter-alpha-trypsin inhibitor heavy chain H1 has not been previously described in the bovine seminal plasma study. These proteins may be contribute to define the type of proteins affecting fertility of male and improve the fertilizing ability of semen in Hanwoo.