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http://dx.doi.org/10.7314/APJCP.2013.14.7.4161

Biomarkers Screening Between Preoperative and Postoperative Patients in Pancreatic Cancer  

Li, Pei (Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University)
Yang, Juan (Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University/Key Laboratory of Environment and Disease-Related Gene, Ministry of Education)
Ma, Qing-Yong (Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University)
Wu, Zheng (Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University)
Huang, Chen (Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University/Key Laboratory of Environment and Disease-Related Gene, Ministry of Education)
Li, Xu-Qi (Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University)
Wang, Zheng (Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.7, 2013 , pp. 4161-4165 More about this Journal
Abstract
Objective: To investigate discriminating protein patterns and potential biomarkers in serum samples between pre/postoperative pancreatic cancer patients and healthy controls. Methods: 23 serum samples from PC patients (12 preoperative and 11 postoperative) and 76 from healthy controls were analyzed using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique combined with magnetic beads-based weak cation-exchange chromatography (MB-WCX). ClinProTools software selected several markers that made a distinction between pancreatic cancer patients and healthy controls. Results: 49 m/z distinctive peaks were found among the three groups, of which 33 significant peaks with a P < 0.001 were detected. Two proteins could distinguish the preoperative pancreatic cancer patients from the healthy controls. About 15 proteins may be potential biomarkers in assessment of pancreatic cancer resection. Conclusion: MB-MALDI-TOF-MS method could generate serum peptidome profiles of pancreatic cancer and provide a new approach to identify potential biomarkers for diagnosis and prognosis of this malignancy.
Keywords
Pancreatic cancer; biomarker; prognosis; mass spectrometry; magnetic beads;
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