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Measurement and Estimation for the Clearance of Radioactive Waste Contaminated with Radioisotopes for Medical Application (의료용 방사성폐기물 자체처분을 위한 방사능 측정 및 평가)

  • Kim, Changbum;Park, MinSeok;Kim, Gi-Sub;Jung, Haijo;Jang, Seongjoo
    • Progress in Medical Physics
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    • v.25 no.1
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    • pp.8-14
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    • 2014
  • The amounts of radioactive wastes to be disposed in the medical institute have been increased due to development of radiation diagnosis and therapy rapidly. They are produced mostly by the very short lived radioisotopes such as $^{18}F$ used in PET/CT, $^{99m}Tc$, $^{123}I$, $^{125}I$ and $^{201}Tl$, etc. IAEA proposed a criteria for the clearance level of waste which depends on the individual ($10{\mu}Sv/y$) and collective dose (1 man-Sv/y), and concentration of each nuclide (IAEA Safety Series No 111-P-1.1, 1992 and IAEA RS-G-1.7, 2004). Radioactive wastes of $^{18}F$, $^{99m}Tc$, $^{123}I$, $^{125}I$ and $^{201}TI$ in the several types of container like Marinelli beaker, vial and plastic, were collected to measure the concentration of the waste of each nuclide in accordance with IAEA criteria. The measurement method and procedure of determining specific activity of the wastes using gamma emitters like MCA, gamma counter and beta emitters were developed. For the efficiency calibration of the detectors, CRM (certified reference material) which has the same dimension and shape was provided by Korea Research Institute of Standards and Science (KRISS). Correction factor of the radioactivity decay was calculated based on the measurement results, and the consideration of mutual relation with theoretical equation. The result of this study will be proposed as ISO standard.

Improvement of Peroxidase Productivity by Optimization of Medium Composition and Cell Inoculum Size in Suspension Cultures of Sweet Potato (Ipomoea batatas) (고구마(Ipomoea batatase)현탁배양에서 배지조성 및 세포접종량의 적정화에 의한 Pemxidase생산성 향상)

  • 곽상수;김수경;정경희;유순희;박일현;유장렬
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.2
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    • pp.91-97
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    • 1994
  • To improve the productivity of peroxidase (POD) of cell line SP-47 derived from cell suspension cultures of sweet potato (Ipomoea batatas (L) Lam.cv White Star), we optimized culture conditions including the composition and concentration of plant growth regulators and carbon source, and the cell inoculum size. When one g (fr wt) of cells was inoculated into 50 mL TL medium supplemented with l mg/L 2,4-D and 30g/L sucrose in 300 mL Erlenmeyer flask at 25$^{\circ}C$ in the dark (100rpm), the POD activity per g cell dry wt was maximized to be about 6,800 units after 25 days of subculture, which was about 30 times higher than that of intact roots of horseradish plants grown in the greenhouse, but the cell growth was maximum after 15 days of subculture. The protein content per g cell dry wt maintained almost plateau and after 25 days of subculture decreased as culture Proceeded further whereas the POD specific activity (unit/mg protein) was about two times higher after subculture and continuously increased from 12 days to the end of cultures (40 days). The POD isozyme patterns showed almost the same regardless of cell growth stage, but some acidic isozymes were slightly increased after 25 days of subculture. These results indicate that POD activity in suspension cultures of sweet potato is closely associated with cell growth and stresses derived from cell culture renditions and medium depletion. Due to its high POD activity the SPL47cell line seems to be suitable for the mass production of POD.

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Labeling and Biodistribution of Re-188-DTPA (Diethylenetriaminepentaaceticacid) (Re-188-DTPA (Diethylenetriaminepentaacetic acid)의 표지와 생체내 분포)

  • Lee, Jin;Kim, Young-Ju;Chang, Young-Soo;Jeong, Jae-Min;Shin, Seung-Ae;Chung, June-Key;Lee, Myung-Chul;Koh, Chang-Soon;Lee, Dong-Soo
    • The Korean Journal of Nuclear Medicine
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    • v.31 no.4
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    • pp.427-432
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    • 1997
  • Re-188 is useful candidate for therapeutic radionuclide because it has a physical half life of 17 hours, contains beta emissions suitable for therapy(maximum energy 2.12MeV) and emits a gamma ray that is suitable for quantitative diagnostic scanning(155keV). To use Re-188 as a radionuclide compound of angioplasty balloon radiotherapy, we investigated the labelling method and biodistribution of Re-188-DTPA We postulated that labeled Re-188-DTPA is preferable because it would be excreted via urinary system more easily than other compounds. To label Re-188 with DTPA, 1ml of 222MBq(6mCi) of Re-188 was added to DTPA solution(DTPA 20mg, $SnCl_2{\cdot}2H_2O$ 10mg, pH 3.5) and boiled at $100^{\circ}C$ for 120min in water bath. pH was adjusted to 5 with 2.3% sodium acetate. Labeling efficiency was measured using TLC-SG(acetone, saline). We evaluated biodistribution of Re-188-DTPA in sacrificed mice at 10 and 60 minutes after injection. We acquired images of kidneys, and drew time-activity curves in normal dogs and rats and calculated Tmax and Tl/2 in rats. The labelling efficiency was 95.7% on average. Labelling of Re-188-DTPA was.stable(90% after 5hours) in vitro at room temperature. According to time-activity curves of dogs and rats, it took 15 to 20 minutes after injection for Re-188-DTPA to be washed out through kidneys. In conclusion, Re-188-DTPA was successfully labeled, Re-188-DTPA was stable in vitro and was excreted early via kidneys in animals. We could recommend Re-188-DTPA as radionuclide of potential use in angioplasty balloon radiotherapy.

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Effects of Dietary Onion Supplementation of on the Physicochemical Properties of Duck Meat (양파의 급여가 오리고기의 이화학적 특성에 미치는 영향)

  • 송영민;진상근;김일석;조용조;김회윤;하경희;남기윤
    • Food Science of Animal Resources
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    • v.24 no.1
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    • pp.66-72
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    • 2004
  • The study was conducted to investigate the effect of dietary onion supplementation on physicochemical properties of duck meat. The ducks were raised for 38 days(1,500 g of live weight) in rice paddle and slaughtered. Samples including breast and leg were stored at 4$^{\circ}C$ for 12hrs and then used as experimental materials. The ducks were allotted into 3 treatments(C-0%, T1-3%, T2-6%) according to dietary supplementation of onion. The ducks meat were stored at 4${\pm}$1$^{\circ}C$ for 12hrs. In proximate analyses, the moisture, crude protein and crude ash of breast muscle were significantly increased(p<0.05) with increasing onion percentages but crude fat content was significantly decreased(p<0.05). The moisture and crude protein of leg muscles were significantly increased(p<0.05) with increasing onion percentages but crude fat and crude ash were significantly decreased(p<0.05). The pH of breast muscle was significantly decreased(p<0.05) with increasing onion percentages, the pH of leg muscle was be highest in T1(p<0.05). Water holding capacity(WHC) significantly increased(p<0.05) with increasing onion percentage in both muscles. Hunter L* was significantly decreased with increasing onion percentage in breast muscles while Hunter b* significantly increased(p<0.05). In fatty acids composition of duck meat, mono-unsaturated fatty acid(MUFA) significantly decreased(p<0.05) while poly-unsaturated fatty acid(PUSFA) and EFA(essential fatty acid) were significantly high(p<0.05) with increasing onion percentage.

The First Record on the Rhynchocypris kumgangensis from Taehwa River, Korea (태화강에서 처음 채집된 금강모치(Rhynchocypris kumgangensis))

  • Byeon, Hwa-Keun
    • Korean Journal of Environment and Ecology
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    • v.30 no.4
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    • pp.745-750
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    • 2016
  • Morphological characteristics and population characteristics of the Rhynchocypris kumgangensis were investigated at Taehwa River from April 2014 to April 2015. This is the first record on R. kumgangensis not only from Guksu stream (the upstream region of Taehwa River, Ulsan-si Ulchu-gun Beomseo-eup Kuksu-ri) but also from the stream flowing into the Gangneungnamdae stream, in the southern part of East Sea, Korea. The morphometric characteristics of R. kumgangensis in the Taehwa River and the Han River are very similar except that the population at Taehwa River showed shorter caudal peduncle length than caudal peduncle depth when compared with the population at Han River. In terms of meristic character the population at Taehwa River showed fewer numbers of scales at the lateral line, above the lateral line and below the lateral line. Other than that, the two populations showed the same meristic characters. The fish cohabiting with R. kumgangensis mainly composed of Zacco platypus (25.2%), Rhynchocypris oxycephalus (24.1%) and Zacco koreanus (21.8%). Length frequency distribution analysis indicated that the length of the group ranged between 45~65 mm and the average length was 54.3 mm. Calculation of the length-weight relation of R. kumgangensis showed the value of constant a as 0.000002 and b as 3.34, and the Condition factor (K) was 1.10 an on average.

Endoscopic Laser Surgery in Laryngeal Carcinoma (후두 악성종양에 대한 내시경적 레이저 수술의 적용)

  • Kim Kwang-Hyun;Sung Myung-Whun;Lee Hyo-Jeong;Lee Dong-Wook;Park Bum-Jung;Seong Weon-Jin;Min Yang-Gi;Lee Chul-Hee;Rhee Chae-Seo;Lee Sang-Jun;Roh Jong-Lyel
    • Korean Journal of Head & Neck Oncology
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    • v.18 no.1
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    • pp.36-40
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    • 2002
  • Background and Objectives: To determine if laser endoscopic microsurgery is a reliable and appropriate approach in the treatment of laryngeal carcinomas. Materials and Methods: Retrospective study of 62 patients treated with CO2 laser from June 1988 to November 2000 at Seoul National University Hospital for laryngeal squamous cell carcinoma. All patients were treated with curative intention. Fifty three untreated patients with laryngeal carcinoma (39 glottic and 14 supraglottic carcinoma patients) had primary carbon dioxide laser microsurgery. Nine radiation failure patients were treated. Postoperative radiotheray was done for 17 patients. Neck dissection was performed simultaneously for 4 supraglottic cases with cervical nodal metastasis. Mean follow-up duration was 40 months. Results: In primary laser surgery group, distribution of tumors (American Joint Committee on Cancer, 1997) were 38 cases with Tl, 13 cases with T2, 2 cases with T3. Cure rate was 88.7%(47/53) and local control rate was 92.5%(49/53). Larynx was preserved in 94%(50/53) of patients. The overall 5-year survival rate(Kaplan-Meier) was 81.5%. In radiation failure group, 56% of patients were recurred after laser surgery. Conclusion: Laser surgery could be a better treatment modality for early laryngeal cancers and selected advanced cases. Additional radiation therapy should be considered if resection margin is not satisfactory.

Morphological and Skeletal Development of Larvae and Juveniles of Entomacrodus stellifer lighti (Herre) (저울베도라치, Entomacrodus stellifer lighti 자치어(仔稚魚)의 형태(形態)와 골격발달)

  • Kim, Yong-Uk;Han, Kyeong-Ho;Kang, Chung-Bae
    • Korean Journal of Ichthyology
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    • v.4 no.2
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    • pp.31-43
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    • 1992
  • The blenniid fish, Entomacrodus stellifer light(Herre) is distributed in the coastal waters of southern Japan and south-eastern Korea. Matured adults of blenniid fish were collected from the rocky shore of Namchun-dong, Nam-gu, Pusan, Korea in July, 1991. The observations were made on the morphology and skeletal development of the reared larvae and juveniles. The lavae reached 5.80mm in mean total length(MTL) in 10 days after hatching. A this time the larvae have absorbed the yolk completely and became postlarvae. Melanophores are distributed on the fin membrane of the lower part of pectoral fin and ventral margins of tail. The larvae averaged 6.20mm in TL and the caudal notochord flex at $45^{\circ}$ in 13 days after hatching. The part of the fin-fold of the future dorsal and anal fins became high. The lavae reached juvenile stage in 31 days after hatching and attained 15.80mm in MTL, and all fin-rays was formed. Ossification of the cranium took place at 6.20mm of MTL(11 days after hatching) in parasphenoid. Vertebrae began to develop from the anterior end to ossify posteriorly. Ossification of all bones nearly completed when the larvae reached to 15.80mm in MTL (31 days after hatching).

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A Study on Knowledge, Educational Needs and Educational Experience for Indwelling Catheter Management in the Families of Home Care Clients (유치도뇨관 삽입 가정간호대상자 가족의 유치도뇨관 관리에 관한 지식, 교육요구 및 교육경험)

  • Kim, Jung-Soon;Lee, Sang-Ju;Kang, In-Soon
    • Journal of Home Health Care Nursing
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    • v.12 no.1
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    • pp.92-116
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    • 2005
  • Purpose: This study was a predicative survey to provide home care clients with indwelling urinary catheters for furnishing basic educational material to their families by analyzing the family members' knowledge, educational needs and educational experience about indwelling catheter management. Method : The subjects consisted of 108 family members who cared for home care clients with indwelling catheters. Data were collected by home care nurses in nine hospitals in Pusan, who directly interviewed with them through questionnaires from Sep. 6th to 30th in 2004. The questionnaires for data collection were developed through pre-survey and reference review. The collected data was analyzed by using frequency, percentile, mean, variation, t-test, ANOVA on SPSS 10.1 package. Results : There were characters of home care clients with indwelling catheters: women(67.6%) were more than men; the average age of them was $69.60{\pm}14.99$ years old; neurogenic and cerebrovascular diseases(80.0%) were the most common disease group; 81.5% of them were totally dependent on others in terms of level of activity. Home care clients' families had these characters: women(76.9%) were more than men; the average age was $54.5{\pm}13.70$ years old; 46.3% of them were parents or sons or daughters in terms of relation with patient; tl1e average care period was $39.8{pm}34.20$ months. Level of knowledge about indwelling catheter management of the family members were 69.8% and its mean were $20.24{\pm}4.53$. Educational needs were 90.9% and its mean were $14.55{\pm}3.56$. Educational experience were 53.3% and its mean were $8.53{\pm}4.30$. 'Complications in using an indwelling catheter', 'Symptoms to call for a home care nurse' and 'Method to attach an indwelling catheter' were high ranked in both educational needs and educational experience. 'Anatomy of urinary tract', 'Functions of urinary tract' and 'Catheter placement' were low ranked in both educational needs and educational experience Level of knowledge about indwelling catheter management of the families showed a significant difference according to their age(F=5.35, p=0.01). Educational needs showed a significant difference according to care period(F=3.06, p=0.04). Conclusion: the family members' level of knowledge and educational experience about indwelling catheter management and their educational were not sufficient while their educational needs were high. In other words although the family members were acknowledged education needs, but the care for the patients with indwelling catheter weren't performed well because of their lack of related knowledge. Therefore systematic educational programs about indwelling catheter management for home care clients and their families should be made on the base of this study.

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Effects of Water Temperature on Egg Development, Hatching and Laval Growth Rearing of the Pacific Cod Gadus macrocephalus (태평양 대구 Gadus macrocephalus 채란, 부화 및 자어성장에 미치는 수온의 영향)

  • Lee, Jeong-Yong;Lee, Chae-Sung;Kim, Wan-Ki;Park, Sang-Un;Min, Byung-Hwa
    • Journal of Aquaculture
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    • v.20 no.4
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    • pp.260-264
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    • 2007
  • We investigated egg collection and the effects of water temperature (4, 7, 10, 13 and $16^{\circ}C$) on egg development, hatching and larval growth of Pacific cod Gadus macrocephalus under laboratory conditions. Fertilized eggs were round in shape ($1.01{\pm}0.03\;cm$) and adhesive to nylon nets. Fertilization rate was 68% by wet method. The time of egg development was negatively proportional to water temperature with the range of $4^{\circ}C$ to $13^{\circ}C$. Eggs hatched only at $7^{\circ}C$ after 288 hours of fertilization and $10^{\circ}C$ after 192 hours. Hatching rate was highest as 65% at $7^{\circ}C$ followed by $34.4^{\circ}C$ at $10^{\circ}C$. Survival rate was 18.3% at $7^{\circ}C$ and 5.2% at $10^{\circ}C$.

Estimation of Genetic Parameters and Trends for Weaning-to-first Service Interval and Litter Traits in a Commercial Landrace-Large White Swine Population in Northern Thailand

  • Chansomboon, C.;Elzo, M.A.;Suwanasopee, T.;Koonawootrittriron, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.5
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    • pp.543-555
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    • 2010
  • The objectives of this research were the estimation of genetic parameters and trends for weaning-to-first service interval (WSI), and litter traits in a commercial swine population composed of Landrace (L), Large White (T), LT, and TL animals in Chiang Mai, Northern Thailand. The dataset contained 4,399 records of WSI, number of piglets born alive (NBA), litter weight of live piglets at birth (LBW), number of piglets at weaning (NPW), and litter weight at weaning (LWW). Variance and covariance components were estimated with REML using 2-trait analyses. An animal model was used for WSI and a sire-dam model for litter traits. Fixed effects were farrowing year-season, breed group of sow, breed group of boar (litter traits), parity, heterosis (litter traits), sow age, and lactation length (NPW and LWW). Random effects were boar (litter traits), sow, permanent environment, and residual. Heritabilities for direct genetic effects were low for WSI (0.04${\pm}$0.02) and litter traits (0.05${\pm}$0.02 to 0.06${\pm}$0.02). Most heritabilities for maternal litter trait effects were 20% to 50% lower than their direct counterparts. Repeatability for WSI was similar to its heritability. Repeatabilities for litter traits ranged from 0.15${\pm}$0.02 to 0.18${\pm}$F0.02. Direct genetic, permanent environment, and phenotypic correlations between WSI and litter traits were near zero. Direct genetic correlations among litter traits ranged from 0.56${\pm}$0.20 to 0.95${\pm}$0.05, except for near zero estimates between NBA and LWW, and LBW and LWW. Maternal, permanent environment, and phenotypic correlations among litter traits had similar patterns of values to direct genetic correlations. Boar genetic trends were small and significant only for NBA (-0.015${\pm}$0.005 piglets/yr, p<0.004). Sow genetic trends were small, negative, and significant (-0.036${\pm}$0.013 d/yr, p<0.01 for WSI; -0.017${\pm}$0.005 piglets/yr, p<0.007, for NBA; -0.015${\pm}$0.005 kg/yr, p<0.01, for LBW; -0.019${\pm}$0.008 piglets/yr, p<0.02, for NPW; and -0.022${\pm}$0.006 kg/yr, p<0.003, for LWW). Permanent environmental correlations were small, negative, and significant only for WSI (-0.028${\pm}$0.011 d/yr, p<0.02). Environmental trends were positive and significant only for litter traits (p<0.01 to p<0.0003). Selection based on predicted genetic values rather than phenotypes could be advantageous in this population. A single trait analysis could be used for WSI and a multiple trait analysis could be implemented for litter traits.