• Journal of Korean Neurosurgical Society
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• v.61 no.3
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• pp.343-351
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• 2018

Diffuse intrinsic pontine glioma (DIPG) is a deadly paediatric brain cancer. Transient response to radiation, ineffective chemotherapeutic agents and aggressive biology result in rapid progression of symptoms and a dismal prognosis. Increased availability of tumour tissue has enabled the identification of histone gene aberrations, genetic driver mutations and methylation changes, which have resulted in molecular and phenotypic subgrouping. However, many of the underlying mechanisms of DIPG oncogenesis remain unexplained. It is hoped that more representative in vitro and preclinical models-using both xenografted material and genetically engineered mice-will enable the development of novel chemotherapeutic agents and strategies for targeted drug delivery. This review provides a clinical overview of DIPG, the barriers to progress in developing effective treatment, updates on drug development and preclinical models, and an introduction to new technologies aimed at enhancing drug delivery.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.25 no.1
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• pp.1-8
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• 2011

In this paper, the method to calculate chromaticity coordinate from spectral power distribution of LED is presented. Also, inverse tri-stimulus algorithm to find mixed luminance of red, green, blue LED from targeted luminance and chromaticity coordinate is proposed. Besides, digital light color control system of LED lamp applied this algorithm has been developed. In experiments, each chromaticity coordinate of red, green, blue LED calculated from this algorithm has relative percentage error of few % to measured values. Digital code is drawn from inverse tri-stimulus algorithm, and measured values of luminance and chromaticity coordinate of LED lamp digitally controlled by this code also have relative percentage error within a few % to targeted luminance and chromaticity coordinate.

• Bulletin of the Korean Chemical Society
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• v.34 no.12
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• pp.3782-3786
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• 2013

Non-small cell lung cancer (NSCLC) is the most common type of lung cancer and that accounts for 85% of lung cancer patients. Although several EGFR-targeted drugs have been developed in the treatment of NSCLC, the clinical efficacy of EGFR-targeted drugs in NSCLC is limited by the occurrence of drug resistance. In this regard, Hsp90 represents great promise as a therapeutic target of cancer due to its potential to simultaneously disable multiple signaling pathways. In this study, we discovered that a natural product, flavokawain B disrupted Hsp90 chaperoning function and impaired the growth of gefitinib-resistant non-small cell lung cancer (H1975). The result suggested that flavokawain B could serve as a potential lead compound to overcome the drug resistance in cancer chemotherapy.

• International Journal of High-Rise Buildings
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• v.5 no.3
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• pp.155-165
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• 2016

A much higher level of seismic performance is needed for supertall buildings due to increased demands for their functional continuities and the recognized needs for their continuing emergence in metropolitan areas. This paper analyzes, compares, and contrasts responses recorded during the 2011 Tohoku-oki Earthquake of different supertall buildings featuring conventional and vibration-controlled engineering systems. The superior performance and advantage of the latter are pointed out, and the typical dynamic properties, response characteristics, and effects on the secondary system are discussed. Ongoing efforts to enhance vibration control performance are described, covering the development of specifications, use of performance curves and targeted displacement design, and methods to find appropriate locations of damper installation resulting in a minimized amount of dampers.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.508-511
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• 2000

In situ hybridization with fluorescent oligonucleotides(FISH) was used to detect and localize microorganisms in the granules of lab-scale upflow anaerobic sludge blanket(UASB) reactors. An UASB reactor was seeded with mesophilically-grown($35^{\circ}\;C$) granular sludge, and thermophilically($55^{\circ}\;C$) operated by feeding with a synthetic wastewater. Sections of the granules were hybridized with 16S rRNA-targeted oligonucleotide probes for Eubacteria, Archaeabacteria, and specific phylogenetic groups of methanogens. FISH clearly showed the layed structure of thermophilic granules, which was consisted of outer bacterial cells and inner archaeal cells. Methanoseata-, Methanosarcina-like cells were also found to be localized inside the granules. These results demonstrated FISH was useful in studying the spatial organizations of methanogens and in situ morphologies and metabolic functions in thermophilic granular sludges.

• BMB Reports
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• v.45 no.1
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• pp.1-6
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• 2012

Hsp90 is one of the most conserved molecular chaperones ubiquitously expressed in normal cells and over-expressed in cancer cells. A pool of Hsp90 was found in cancer mitochondria and the expression of the mitochondrial Hsp90 homolog, TRAP1, was also elevated in many cancers. The mitochondrial pool of chaperones plays important roles in regulating mitochondrial integrity, protecting against oxidative stress, and inhibiting cell death. Pharmacological inactivation of the chaperones induced mitochondrial dysfunction and concomitant cell death selectively in cancer cells, suggesting they can be target proteins for the development of cancer therapeutics. Several drug candidates targeting TRAP1 and Hsp90 in the mitochondria have been developed and have shown strong cytotoxic activity in many cancers, but not in normal cells in vitro and in vivo. In this review, recent developments in the study of mitochondrial chaperones and the mitochondria-targeted chaperone inhibitors are discussed.

• Biomolecules & Therapeutics
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• v.22 no.4
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• pp.267-274
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• 2014

Drug development and preclinical trials are challenging processes and more than 80% to 90% of drug candidates fail to gain approval from the United States Food and Drug Administration. Predictive and efficient tools are required to discover high quality targets and increase the probability of success in the process of new drug development. One such solution to the challenges faced in the development of new drugs and combination therapies is the use of low-cost and experimentally manageable in vivo animal models. Since the 1980's, scientists have been able to genetically modify the mouse genome by removing or replacing a specific gene, which has improved the identification and validation of target genes of interest. Now genetically engineered mouse models (GEMMs) are widely used and have proved to be a powerful tool in drug discovery processes. This review particularly covers recent fascinating technologies for drug discovery and preclinical trials, targeted transgenesis and RNAi mouse, including application and combination of inducible system. Improvements in technologies and the development of new GEMMs are expected to guide future applications of these models to drug discovery and preclinical trials.

• English Language & Literature Teaching
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• v.18 no.2
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• pp.107-124
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• 2012

The current research dealt with two areas which have been at the center of attention in the field of second language acquisition (SLA): recasts and intake. Although these two are continuously discussed in many studies, not enough research has addressed how they are related. Hence, the current research sought to identify the relationship between learner intake of language forms delivered in the form of recasts in adult EFL classrooms and learner immediate responses to recasts (i.e., uptake). The data were collected from 11 classrooms. A total of 221 recast episodes were observed and learners' responses to recasts were analyzed. On the basis of the recast episodes, multiple-choice recognition tests were developed and administrated to measure learner intake of the linguistic forms targeted by recasts. The study found that a half of recasts brought about learner intake. In addition, the learners responded to recasts in many different ways and their ways of responding were closely related to their intake of linguistic forms targeted by recasts.

• Plant Biotechnology Reports
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• v.5 no.1
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• pp.9-17
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• 2011

With the development of next-generation sequencing technology, ever-expanding databases of genetic information from various organisms are available to researchers. However, our ability to study the biological meaning of genetic information and to apply our genetic knowledge to produce genetically modified crops and animals is limited, largely due to the lack of molecular tools to manipulate genomes. Recently, targeted cleavage of the genome using engineered DNA scissors called zinc finger nucleases (ZFNs) has successfully supported the precise manipulation of genetic information in various cells, animals, and plants. In this review, we will discuss the development and applications of ZFN technology for genome engineering and highlight recent reports on its use in plants.

• The Korean Journal of Emergency Medical Services
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• v.5 no.1
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• pp.53-61
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• 2001

The purpose of the study is to evaluate the effectiveness and competence level of trainees of Cardiopulmonary Resuscitation training targeted for policeman. Data were collected from 113 policemen by questionnaire on Feb, 2001. The data were analyzed by percentage, mean, paired t-test using SPSS program. The results of this study were as follows : 1. 17 questions score increase, and 1 question score decreased. 2 questions didn't change their score. 2. In post test, knowledge level of trainees increased compared to that of pretest(t=-13.055, p= .000). 3. Competence level also increased(t=-13.055, p= .000).