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http://dx.doi.org/10.1007/s11816-010-0161-0

Targeted genome engineering via zinc finger nucleases  

Kim, Seok-Joong (ToolGen, Inc., Biotechnology Incubating Center, Seoul National University)
Kim, Jin-Soo (Department of Chemistry, Seoul National University)
Publication Information
Plant Biotechnology Reports / v.5, no.1, 2011 , pp. 9-17 More about this Journal
Abstract
With the development of next-generation sequencing technology, ever-expanding databases of genetic information from various organisms are available to researchers. However, our ability to study the biological meaning of genetic information and to apply our genetic knowledge to produce genetically modified crops and animals is limited, largely due to the lack of molecular tools to manipulate genomes. Recently, targeted cleavage of the genome using engineered DNA scissors called zinc finger nucleases (ZFNs) has successfully supported the precise manipulation of genetic information in various cells, animals, and plants. In this review, we will discuss the development and applications of ZFN technology for genome engineering and highlight recent reports on its use in plants.
Keywords
Zinc finger nucleases; Genome engineering Gene targeting; Genetically modified organism;
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