• Journal of the Korea institute for structural maintenance and inspection
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• v.24 no.2
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• pp.9-14
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• 2020

Four developed patents have applied for a new type of Composite Cyclone Scrubber followed by the previous research (Cho and Kim, 2017), including dust reducing fan with filters. Regarding target installation and maintenance cost, 64% reduction for investment costs (6.2 billion won vs. 17 billion won) compared to existing road pollution reduction system, while social benefit costs increase by 43% compared to existing road pollution reduction measures (72.6 billion won vs. 50.8 billion won). The composition of the device is an air blower type spiral guide vane, and an injection pressure collecting dust efficiency. A nozzle varies Injection angle and contact range, spray liquid species (waterworks, salty water). The proposed patent tests are circulation water Time-by-Time Spray and collected 41.4% more increased micro dust since the sprayed water meets contaminated gas due to the 45° degree colliding, which is 141% increased conventional dust collector. (Ratio of collection over 85%). As regards the source of collection liquid, circulated rainwater and well water, we expect a huge amount of energy and economically saved eco-friendly system in our patent. Finally, the guided vane and metal filter reduced over 90% micro-dust, while sprayed water cleans the vane and filters, resultantly minimizing the maintenance budget. The preliminary evaluations of the developed design make it possible to reduce not only cheaper maintenance budget due to the characteristic water spraying but the cost of water comes from mainly rain and underground.

• Ecology and Resilient Infrastructure
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• v.3 no.4
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• pp.285-293
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• 2016

This study conducted the evaluations of stable channel and physical disturbance improvement for optimal construction of river structures by focusing on Wonju River. A stable slope was analyzed sectionally for stable channel design, and in order to satisfy the stable slope, river structure improvement scenarios were deduced. Accordingly, through physical disturbance improvement evaluation for each scenario, the study extracted the optimal scenario for stable channel design and physical disturbance improvements. The changes in physical habitat were analyzed when river structure improvements were made for stable channel design and physical disturbance improvement. Zacco koreanus, an indicator fish of the soundness of the aquatic ecosystem, was selected as a restoration target species by investigating the community characteristics of fish fauna and river environments in the Wonju River. The habitat suitability was investigated by the PHABSIM model with the habitat suitability index of Zacco koreanus. The results of the prediction evaluation showed a slight decrease in habitat suitability and weighted usable area. However, it was not influenced by the improvements in the river structure. The study suggested river structure arrangement methods that can improve physical soundness and safety of Wonju River, and confirmed that there were no effects to the changes in the physical habitat.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.3 no.4
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• pp.269-278
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• 2005

In order to produce only a pH-controlled solution without discharging any unwanted solution, this work has developed a continuous electrolytic system with a pH-adjustment reservoir being placed before an ion exchange membrane-equipped electrolyzer, where as a target solution was fed into the pH-adjustment reservoir, some portion of the solution in the pH-adjustment reservoir was circulated through the cathodic or anodic chamber of the electrolyzer depending on the type of the ion exchange membrane used, and some other portion of the solution in the pH-adjustment reservoir was discharged from the electrolytic system through the other counter chamber with its pH being controlled. The internal circulation of the pH-adjustment reservoir solution through the anodic chamber in the case of using a cation exchange membrane and that through the cathodic chamber in the case of using an anion exchange membrane could make the solution discharged from the other counter chamber effectively acidic and basic, respectively. The phenomena of the pH being controlled in the system could be explained by the electro-migration of the ion species in the solution through the ion exchange membrane under a cell potential difference between anode and cathode and its consequently-occurring non-charge equilibriums and electrolytic water- split reactions in the anodic and cathodic chambers.

• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.612-616
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• 2007

오랫동안 홍수조절, 용수공급, 전력생산, 레저 활동을 위해 건설된 댐들은 하천이나 호소의 생태적 기능보다는 치수 및 이수기능이 중요시되면서 운영 관리되어 왔다. 하지만 최근 들어 선진국을 중심으로 환경 및 생태의 중요성이 인식되면서 하천생태계 보호를 위한 환경유량 산정이 언급되고 있는데 이는 하천이 동 식물의 서식처로서 적절한 수질뿐만 아니라 수심, 유속, 하상재료, 먹이원, 어류를 보호할 수 있는 휴식 및 은신처 등의 다양한 조건을 제공할 수 있어야 한다고 제시되고 있다. 이들 조건 중 유량(Flow)은 하천에서 생물집단을 구성하는데 있어 물리적 서식조건의 주요한 결정인자로 작용하는데, 댐 하류하천의 자연유량(natural flow)을 변화시키면 생태학적 측면에서 기대치 않던 영향을 야기할 수 있다. 따라서 댐 건설 전 후의 자연유량과 조절유량 사이에서 생태학적 어류조사연구를 통한 차이점을 찾는 것은 매우 중요하다고 볼 수 있다. 본 연구의 최종목적은 수계 내 댐 건설 전 후의 어류군집과 유량 등 수리조건과의 연관성을 분석하여 하천구간별로 어류생태에 적합한 유량을 산정하는 것이다. 조사대상 구간은 금강본류를 대상으로 하되 용담댐과 대청댐을 중심으로 하여 10개 구간을 선정하였고. 과거로부터 2000년도까지와 용담댐 건설후인 $2002{\sim}2004$년도에 조사된 어류생태자료를 활용하였다. 용담댐 상류로부터 대청댐 하류에 이르기까지 전 구간을 대상으로 조사된 자료를 분석한 결과 총 20과 82종이 출현하였고, 이중 종 45종(54.9%)이 잉어과에 해당하며 미꾸리과(6종), 동자개과(4종), 망둑어과(5종)를 제외한 대다수의 분류군(Family)들은 단일 또는 2종으로만 구성되었다. 이들 중 천연기념물 제 259호로 지정되어 법적으로 보호되고 있는 어름치(금강에서 서식한 어름치를 따로 천연기념물 238호로 지정함)를 비롯하여 다묵장어를 포함한 7종의 멸종위기종이 확인되었다. 또한 각시붕어 등 총 29종(39.4%)의 높은 한국고유종 출현과 6종의 외래도입종도 확인되었다. 금강의 중 상류에 해당하는 구역을 포함하는 대청댐 상 하류 구간에서 대청댐 건설 전(1980년 이전)을 포함하는 2000년 이전 조사 자료가 가장 다양한 어류상과 특이어종(멸종위기종 및 한국고유종)을 보였고, 최근자료$(2002{\sim}2004)$를 살펴볼 때 용담댐 상 하류에서보다 대청댐 상 하류에서 멸종위기종(7종$\rightarrow$1종) 및 한국고유종(28종$\rightarrow$16종)의 출현감소와 외래도입종의 출현증가(1종$\rightarrow$6종)와 같은 주요한 어류군집 변화를 보였다. 이는 댐 건설에 따라 주로 계류성 어종을 중심으로 정수역 구간에서 인근 지류로 이동하게 되고 일부는 제한된 서식공간과 하천교란으로 인해 개체군이 극감하거나 일부 소멸된 종이 발생하였을 것으로 사료된다. 실측 유량자료 분석에 의하면 홍수기 최대유량 변화는 크지 않으나, 갈수기 최소유량은 크게 증가한 것을 알 수 있었고, 대다수 어류의 산란기인 봄철(5월, 6월) 최소유량은 증가하였으나, 최대유량은 감소한 것으로 분석되었다. 이러한 변화는 어류생태계에 긍정적인 면과 부정적인 면이 있으며, 향후 이러한 영향을 분석하기 위해 선택어종(Target species)별 생태환경 서식조건을 확인할 수 있는 조사기법 및 자료구축이 절실히 필요한 실정이다.

• Korean Journal of Microbiology
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• v.45 no.3
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• pp.268-274
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• 2009

A photosynthetic cyanobacterium Arthrospira platensis, well known for health food supplement, was studied as a target species for atmospheric $CO_2$ removal as well as biomass production. Although the biomass of A. platensis was massively produced in many countries, the recovery cost of its biomass is still high. The purpose of this study was to develop the A. platensis mutant strains which have enhanced growth rate and floatation activity to reduce the recovery cost. A. platensis KCTC AG20590 was treated with 0.24% ethyl methanesulfonate (EMS) for 20 min at room temperature. The mutant strain A. platensis M20CJK3 was finally selected by its morphological and physiological features. The morphology of the mutant A. platensis M20CJK3 was changed from loose-coiled form to tight-coiled form showing short pitch. The growth and $CO_2$ uptake rate of A. platensis M20CJK3 were improved about 15% and 17% compared with A. platensis KCTC AG20590, respectively. The floatation activity of A. platensis M20CJK3 was enhanced in 2-fold compared with that of A. platensis KCTC AG20590. Soluble proteins extracted from two strains were analyzed by two dimensional electrophoresis (2-DE) and MALDI-TOF MS/MS. Among 15 protein spots induced in 2-DE analysis, two spots were the proteins related to photosynthesis and electron transfer system of the other cyanobacteria. As a consequence, it seems that the tight-coiled mutant A. platensis M20CJK3 has an advantage of high growth rate and floatation activity which are beneficial for the mass cultivation and recovery.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2005.06a
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• pp.101-109
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• 2005

In order to produce only a pH-controlled solution without discharging any unused solution, this work has developed a continuous electrolytic system with a pH-adjustment reservoir being placed before an ion exchange membrane-equipped electrolyzer, where as a target solution was fed into the pH-adjustment reservoir, some portion of the solution in the pH-adjustment reservoir was circulated through the cathodic or anodic chamber of the electrolyzer depending on the type of the ion exchange membrane used, and some other portion of the solution in the pH-adjustment reservoir was discharged from the electrolytic system through other counter chamber with its pH being controlled as acid or base. The phenomena of the pH being controlled in the system could be explained by the electro-migration of the ion species in the solution through the ion exchange membrane under a cell potential difference between anode and cathode and its consequently-occurring non-charge equilibriums and electrolytic water- split reactions in the anodic and cathodic chambers.

• Journal of the Korean Society for Marine Environment & Energy
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• v.12 no.2
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• pp.91-95
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• 2009

To understand the effect of attachment substrate on the growth of benthic microalgae, we experimentally investigated the growth of benthic microalgae Nitzschia sp. (Jinhae Bay strain) with additions of glass beads in different sizes. The glass beads used in this study are 0.09-0.15 mm (G1), 0.25-0.50 mm (G2), 0.75-1.00 mm (G3) and 1.25-1.65 mm (G4). No addition of glass beads used as controls. Highest specific growth rate (0.37/day) and maximum cell density ($9,232{\pm}840$ cells/mL) of Nitzschia sp. showed at the smallest glass beads (G1), and the specific growth rate and maximum cell density were decreasing with increasing size of glass beads (specific growth rate and maximum cell density of G4 was 0.24/day and $6,397{\pm}524$ cells/mL, respectively). Moreover, specific growth rate of the control experiment (0.23/day) was significantly lower than their of G1 to G3 experiment. The results indicated that the attachment substrate for benthic microalgae as Nitzschia sp. is important factor which affecting the growth rate as well as cell density. Therefore, the physiological experiment of benthic microalgae seems to be necessary to preliminary experiment, which is addition or not of the attachment suitable substrate and the grain size for the target species of benthic microalgae.

• KSBB Journal
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• v.18 no.3
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• pp.190-196
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• 2003

An analytical system detecting DNA particularly utilizing a concept of membrane strip chromatography initially applied to home-version tests for, such as, pregnancy and ovulation has been developed. We have chosen S. typhimurium as model analyte among food-contaminating microorganisms that occurred in high frequencies, and invA gene, as a detection target, specific to Salmonella species. This gene was able to be amplified by PCR under optimal conditions employing newly designed primers in our laboratory. The PCR product was specifically measured via hybridization between the analyte and a DNA probe, which was a totally different feature from the conventional gel electrophoresis detecting the products based only on the molecular size. It is notable thar the DNA probe sequence was specially designed such that no separation of excess primers present after PCR was required. This was immobilized on a nitrocellulose (NC) membrane via streptavidin-biotin linkage minimizing a steric effect when the hybridization with the amplified DNA took place. The analyrical system detected the microorganism in a concentration of minimum $10^3$ cfu/mL (i.e., 10 cells per system), estimated from the standard curve, 20 to 40 minutes after adding the sample. This sneitivity was approximately 10 times higher than that of gel electrophoresis as an analytical tool conventionally used. Furthermore, the assay was able to be run at room temperature, which would ofter an extra advantage to users.

• Korean Journal of Poultry Science
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• v.47 no.1
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• pp.9-19
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• 2020

Avian influenza (AI) viruses are highly contagious viruses that infect many bird species and are zoonotic. Ducks are resistant to the deadly and highly pathogenic avian influenza virus (HPAIV) and remain asymptomatic to the low pathogenic avian influenza virus (LPAIV). In this study, we identified common differentially expressed genes (DEGs) after a reanalysis of previous transcriptomic data for the HPAIV and LPAIV infected duck lung cells. Microarray datasets from a previous study were reanalyzed to identify common target genes from DEGs and their biological functions. A total of 731 and 439 DEGs were identified in HPAIV- and LPAIV-infected duck lung cells, respectively. Of these, 227 genes were common to cells infected with both viruses, in which 193 genes were upregulated and 34 genes were downregulated. Functional annotation of common DEGs revealed that translation related gene ontology (GO) terms were enriched, including ribosome, protein metabolism, and gene expression. REACTOME analyses also identified pathways for protein and RNA metabolism as well as for tissue repair, including collagen biosynthesis and modification, suggesting that AIVs may evade the host defense system by suppressing host translation machinery or may be suppressed before being exported to the cytosol for translation. AIV infection also increased collagen synthesis, showing that tissue lesions by virus infection may be mediated by this pathway. Further studies should focus on these genes to clarify their roles in AIV pathogenesis and their possible use in AIV therapeutics.

• Proceedings of the Korea Crystallographic Association Conference
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• 2003.05a
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• pp.18-18
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• 2003

Thioredoxin (Trx) is a small redox protein that is ubiquitously distributed from achaes to human. In diverse organisms, the protein is involved in various physiological roles by acting as electron donor and regulators of transcription and apoptosis as well as antioxidants. Sequences of Trx within various species are 27～69% identical to that of E. coli and all Trx proteins have the same overall fold, which consists of central five β strands surrounded by four α helices. The N-terminal cysteine in WCGPC motif of Trx is redox sensitive and the motif is highly conserved. Compared with general cysteine, the N-terminal cysteine has low pKa value. The result leads to increased reduction activity of protein. Recently, novel thio.edoxin-related protein (TRP14) was found from rat brain. TRP14 acts as disulfide reductase like Trx1, and its redox potential and pKa are similar to those of Trx1. However, TRP14 takes up electrons from cytosolic thioredoxin reductase (TrxR1), not from the mitochondrial thioredoxin reductase (TrxR2). Biological roles of TES14 were reported to be involved in regulating TNF-α induced signaling pathways in different manner with Trx1. In depletion experiments, depletion of TRP14 increased TNF-α induced phosphorylation and degradation of IκBα more than the depletion Trx1 did. It also facilitated activation of JNK and p38 MAP kinase induced by TNF-α. Unlike Trx1, TRP14 shows neither interaction nor interference with ASK1. Here, we determined three-dimensional crystal structure of TRP14 by MAD method at 1.8Å. The structure reveals that the conserved cis-Pro (Pro90) and active site-W-C-X-X-C motif, which may be involved in substrate recognition similar to Trx1 , are located at the beginning position of strand β4 and helix α2, respectively. The TRP14 structure also shows that surface of TRP14 in the vicinity of the active site, which is surrounded by an extended flexible loop and an additional short a helix, is different from that of Trx1. In addition, the structure exhibits that TRP14 interact with a distinct target proteins compared with Trx1 and the binding may depend mainly on hydrophobic and charge interactions. Consequently, the structure supports biological data that the TRP14 is involved in regulating TNF-α induced signaling pathways in different manner with Trx1.