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http://dx.doi.org/10.5536/KJPS.2020.47.1.9

Identification of Differentially Expressed Genes in Ducks in Response to Avian Influenza A Virus Infections  

Ndimukaga, Marc (Department of Animal Biotechnology, College of Agricultural and Life Sciences, Jeonbuk National University)
Won, Kyunghye (Department of Animal Biotechnology, College of Agricultural and Life Sciences, Jeonbuk National University)
Truong, Anh Duc (Department of Biochemistry and Immunology, National Institute of Veterinary Research)
Song, Ki-Duk (Department of Animal Biotechnology, College of Agricultural and Life Sciences, Jeonbuk National University)
Publication Information
Korean Journal of Poultry Science / v.47, no.1, 2020 , pp. 9-19 More about this Journal
Abstract
Avian influenza (AI) viruses are highly contagious viruses that infect many bird species and are zoonotic. Ducks are resistant to the deadly and highly pathogenic avian influenza virus (HPAIV) and remain asymptomatic to the low pathogenic avian influenza virus (LPAIV). In this study, we identified common differentially expressed genes (DEGs) after a reanalysis of previous transcriptomic data for the HPAIV and LPAIV infected duck lung cells. Microarray datasets from a previous study were reanalyzed to identify common target genes from DEGs and their biological functions. A total of 731 and 439 DEGs were identified in HPAIV- and LPAIV-infected duck lung cells, respectively. Of these, 227 genes were common to cells infected with both viruses, in which 193 genes were upregulated and 34 genes were downregulated. Functional annotation of common DEGs revealed that translation related gene ontology (GO) terms were enriched, including ribosome, protein metabolism, and gene expression. REACTOME analyses also identified pathways for protein and RNA metabolism as well as for tissue repair, including collagen biosynthesis and modification, suggesting that AIVs may evade the host defense system by suppressing host translation machinery or may be suppressed before being exported to the cytosol for translation. AIV infection also increased collagen synthesis, showing that tissue lesions by virus infection may be mediated by this pathway. Further studies should focus on these genes to clarify their roles in AIV pathogenesis and their possible use in AIV therapeutics.
Keywords
avian influenza virus; ducks; differentially expressed genes; transcriptomic response;
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