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In vitro Cytotoxicity Evaluation of Polydimethylsiloxane as a Biosensor Coating Material (바이오센서 코팅용 Polydimethylsiloxane의 생체외 세포독성 평가)

  • Park, Subeom;Lee, Jonghwan;Na, Kyunga;Jung, Jaeyeon;Kim, Myungjin;Park, Sungjae;Hyun, Jinho
    • Journal of Adhesion and Interface
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    • v.10 no.2
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    • pp.77-83
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    • 2009
  • PDMS was selected for a coating material of implantable biosensors and the cytotoxicity of extracts released from a polymer was evaluated using ISO 10993-5, Biological evaluation of medical devices-Part 5: Tests for in vitro cytotoxicity. Organo-tin was used as a positive control and a medium without serum was used as a negative control. Materials extract were prepared by incubating specimens in RPMI medium without serum ($125{\mu}L/cm^2$) for 24 h, 1 week and 6 weeks at $38^{\circ}C$. The evaluation of cytotoxicity was performed by two different methods : 1) seeding cells with extracts at the beginning 2) incubating extracts with cell sheets already formed on the plate. Both cell morphology and MTT numerical data were shown for the confirmation of cytotoxicity and cell spreading on the surface of PDMS.

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The Arch Type PV System Performance Evaluation of Multi Controlled Inverter for Improve the Efficiency (효율개선을 위한 다중제어 인버터방식의 아치형 PV System 성능 분석)

  • Lee, Mi-Yong;Park, Jeong-Min
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.11
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    • pp.5452-5457
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    • 2012
  • It is saving material cost and construction cost by replacing conventional building materials, and It has advantages for aesthetic value. In the Europe, the United States, Japan and other country research about BIPV is actively being carried out and marketability is also being infinity expanding. Arch type PV systems efficiency characteristics is different depending on PV array's directly connection, parallel connection and arches angle, but is a lack of analysis on this nowadays. When the arch type PV system design up, they consider about aesthetic value and they didn't consider about generation efficiency. In this paper, we try to improve the efficiency through optimization of arch type PV system and estimation of the efficiency parameters of the arch type PV system, such as latitude, longitude, temperature, insolation, arch angle and each kind loss from system organization. For improving Arched PV system efficiency, proposed multiple control inverter system, and using simulation tool of Arched PV system "Solar pro", flat-plate type and many arch type PV system configuration the driving characteristics were compared and analyzed.

Study of Examples for Air Bag Non-deployment Including Rear Collision and Failure Phenomenon by Damage of Control Parts in Vehicle Air Bag (자동차 에어백의 제어부품 불량에 의한 고장현상 및 후방 추돌에 관련된 에어백 미전개에 대한 사례 연구)

  • Lee, Il Kwon;Kim, Young Gyu;Moon, Hak Hook
    • Journal of the Korean Institute of Gas
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    • v.16 no.6
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    • pp.102-106
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    • 2012
  • The purpose of this paper is to study the failure cases in relation to system of Air Bag in vehicle happened in the field. In the first example, it was separated the soldering parts connected the wire pin between air bag module and clock spring of air bag. Whenever the pin shake by the car's vibration, the driver verified the malfunction phenomenon appeared air bag warning lamp on instrument panel in front of driver's seat. in car inside room. The second example, it verified the warning lamp lighting phenomenon of air bag by produced the circuit plate non-contacting of single an element in air bag electronic control unit. The third example, it verified the light of air bag warning indicator lamp by separated with soldering parts connecting inner pin and resistance terminal of seat belt pretensioner using passenger seat. The fourth example, when the passenger car crash a back of truck, the former bumper get jammed under the latter as the roof height of car low less than that. Therefore, the impact of Car's collision verified that don't transfer with body frame of vehicle because of no attachment impact sensor in it.

Characterization of Melanin-concentrating Hormone from Olive Flounder (Paralichthys olivaceus) (양식넙치 멜라닌 농축 호르몬의 특성)

  • Chung, In Young;Jeon, Jeong Min;Song, Young Hwan
    • Journal of Life Science
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    • v.28 no.3
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    • pp.284-292
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    • 2018
  • The melanin-concentrating hormone (MCH), a cyclic hypothalamic peptide composed of 17 amino acids, was initially identified in chum salmon (Oncorhynchus keta) as a regulator of pigmentation. Mammalian MCHs are cyclic hypothalamic peptides composed of 19 amino acids that regulate food intake and energy homeostasis. The present study examined not only MCH expression of different tissues but also the melanohore aggregation and intracellular $Ca^{2+}$ influx of fMCH and the other MCH. Real-time qPCR showed that MCH expressed specially in the brain, gonad, and ovary, and expression of MCH was observed during the developmental stages. In the application of synthetic fMCH and both types of synthetic fMCH, dN-fMCH and dC-fMCH, scale melanophore induced significant changes in aggregation activity with various concentrations of MCH. Also, compared to hMCH and sMCH, fMCH exhibited a 36~99.85% increase in relative potency (%), whereas aggregation of dN-fMCH and dC-fMCH remained in a high concentration. However, dispersion was induced rapidly according to be low concentration of dN-fMCH and dC-fMCH. We show that fMCH and its derivates were bound human MCHR1 and rat MCHR expressed in HEK293T cells with nano-molar affinity and are likely to be ligand-induced to mobilize intracellular $Ca^{2+}$. These results may provide new ligands for binding assay with MCHew ligands, as a structure similar to the mammalian MCH structure was discovered in fish. Once the fMCH receptor system is in place, it can be compared to the MCH system of mammals in terms of MCH function.

Effects of Dietary Green Tea on Meat Quality and Physico-chemical Properties of Broiler during Cold Storage (녹차 산물 급여가 계육의 저장 기간 중 육질과 이화학적 특성에 미치는 영향)

  • Jeong, J.Y.;Kim, G.D.;Jung, E.Y.;Hwang, Y.H.;Kim, S.H.;Kang, K.H.;Choi, Y.H.;Joo, S.T.;Park, G.B.
    • Korean Journal of Poultry Science
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    • v.36 no.1
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    • pp.77-84
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    • 2009
  • The objective of the study was to investigate the effects of dietary green tea on chicken meat quality and fatty acid content. Broilers were fed one of the four dietary treatments containing 0, 0.5, 1 and 3 % green tea for 10 days. Crude moisture, crude fat, crude ash, pH, meat color (CIE $L^*$, $a^*$, $b^*$) and fatty acid composition were measured in chicken breast meat during cold-storage. Crude fat did not differ significantly among treatments. Dietary green tea increased the amount of unsaturated fatty acids and decreased that of saturated fatty acids in chicken meat, resulting in increased ratios of unsaturated fatty acids to saturated fatty acids. The amount of linoleic acid (18:2) and linolenic acid (18:3) was increased with increasing dietary green tea. Lightness ($L^*$), pH, TBARS, VBN, and total plate count were not significantly different during storage. Dietary green tea did not affect storage properties of chicken meat. The results of this study imply that dietary with green tea may have positive effects on chicken meat quality.

Effect of Cooking and Packaging Methods on the Thiobarbituric Acid Reactive Substances and Cholesterol Oxidation Products of Turkey Thigh Meat Patties During Storage (가열방법과 포장조건이 칠면조육 패티의 저장중 지방산화와 콜레스테롤 산화물에 미치는 효과)

  • Hur, S.J.;Joo, S.T.;Park, G.B.;Kim, I.S.;Jin, S.K.
    • Journal of Animal Science and Technology
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    • v.46 no.3
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    • pp.397-404
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    • 2004
  • Turkey thigh meats with skin were ground twice through a 3-mm plate and patties were prepared. Patties were cooked using 5 different methods(oven cooking, pan frying, oil deep frying, boiling, and microwaving) to an internal temperature of 85${\sim}90^{\circ}C$ and packaged in either oxygen permeable PVC zipperbags or oxygen impermeable PVDC bags. The samples were analyzed for thiobarbituric acid reactive substances(TBARS) and cholesterol oxidation products(COPs) after storage at $4^{\circ}C$. The TBARS of cooked meat increased during the storage regardless of cooking methods, vacuum packaged thigh meat produced less TBARS and COPs than the aerobically packaged samples. At the beginning of storage, aerobically packaged meat cooked by boiling method produced higher TBARS than that of others. However, the TBARS of microwaved meat with vacuum packaging increased rapidly after 3 days of storage at $4^{\circ}C$. The amount of total COPs in cooked thigh meat increased linearly with storage time. The level of total COPs in aerobically packaged cooked meat was higher than the vacuum-packaged meat. Microwave produced higher level of total COPs in meat than other cooking methods during storage.

Bactericidal Efficacy of Fumagari OPP®, Fumigant Against Escherichia coli and Salmonella typhimurium (훈증소독제, Fumagari OPP®의 Escherichia coli와 Salmonella typhimurium에 대한 살균효과)

  • Park, Eun-Kee;Kim, Yongpal;Yu, Eun-Ah;Yoo, Chang-Yeol;Choi, Hyunju;Kim, Suk;Lee, Hu-Jang
    • Journal of Food Hygiene and Safety
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    • v.28 no.3
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    • pp.234-240
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    • 2013
  • This test was performed to evaluate the bactericidal efficacy of Fumagari OPP$^{(R)}$, fumigation disinfectant, containing 20% ortho-phenylphenol against Escherichia coli (E. coli) and Salmonella typhimurium (S. typhimurium). In preliminary tests, both E. coli and S. typhimurium working culture suspension number (N value) was $4.0{\times}10^8$ CFU/mL. And all of the colony numbers on the carriers exposed the fumigant (n1, n2, n3) were higher than 0.5N1 (the number of bacterial test suspentions by pour plate method), 0.5N2 (the number of bacterial test suspentions by filter membrane method) and 0.5N1, respectively. In addition, the mean number of bacteria recovered on the control-carriers (T value) was $3.4{\times}10^6$ CFU/mL. In the bactericidal effect of the fumigant, the reduction number of S. typhimurium and E. coli (d value) was 5.26 and 5.64 logCFU/mL, respectively. According to the French standard for the fumigant, the d value for the effective bactericidal fumigant should be over than 5 logCFU/mL. With the results of this study, Fumagari OPP$^{(R)}$ has an effective bactericidal activity, then the fumigant can be applied to disinfect food materials and kitchen appliances contaminated with pathogenic bacteria.

Identifying the Subjects of Nutrition Education for Junior and Senior High School Students (중.고등학생 대상 영양교육 현황 분석을 통한 영양교육 주제 도출)

  • Kang Yeo-Hwa;Yang Il-Sun;Kim Hye-Young;Lee Hae-Young
    • Journal of Nutrition and Health
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    • v.37 no.10
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    • pp.938-945
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    • 2004
  • The purposes of this study were to survey the present situation of nutrition education in junior/senior high school, to analyze the difference between the favorite subjects on students and the existing subject done by dietitians on nutrition education, and then to identify the subjects of nutritional education for junior/senior high school students. Self-administered questionnaires were given to dietitians and students and 142 of the 200 dietitians (71.0%) and 479 of the 564 students (84.9%) responded to the questionnaire on the nutritional education. According to the survey results, 76.1 % of school dietitians currently were doing nutrition education for students while the other 23.9% didn't have such education programs even though they recognized the necessity strongly. The target of nutrition education was student rather than parents or teacher generally and the principal measuring method for nutrition education effect was change of plate waste and leftover'. It was found that significant difference in the collector and source of nutritional education information between junior and senior high school dietitians (p< .05) . About ninety-eight percentage of dietitians felt that nutrition education should keep pace with meal service and this meant that almost respondent recognized the necessity of nutrition education for students. Because the most serious one among operational problems of nutrition education program was lack of enough time owing to too many affairs as dietitian, junior and senior high schools should support so that dietitians would share enough time for nutrition education. And also, a gap was existed between actual and favorite subjects on nutrition education. Significant differences existed in the following subjects; 'basic concept of food and nutrition', 'disease and nutrition', 'food culture in the world', 'cooking and culinary art', 'food of the 24 divisions of the year in the lunar calendar', 'food waste and environmental issue', 'food habit and food behavior', 'exercise and nutrition' (p< .001) 'table manner', 'food and individual sanitation', 'recommended dietary allowance' (p < .01) and 'menu planning' (p< .05) .Finally, 24 subjects of nutritional education were selected based on the survey results. These subjects would be expected to be able to contribute the effective and interesting nutrition education program for junior and senior high school students.

Analysis of genetic characteristics and development of substrate for cultivation in brown strains of Flammulina velutipes (갈색팽이버섯의 유전적 특성검정 및 배지재료 개발)

  • Choi, Jae-Sun;Noh, Jae-Goan;Jang, Who-Bong;Choi, Seong-Yeol;Min, Kyoung-Beom;Kong, Won-Sik
    • Journal of Mushroom
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    • v.5 no.1
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    • pp.13-20
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    • 2007
  • Analysis of genetic characteristics of Flammulina velutipes showed that strains have a range of 85% in genetic distribution diagram. According to this result, we divided these strains into five groups. In experiment of determinating the optimum media and condition in cultivating F. velutipes, we found the optimum temperature and pH range for hypha growth were $25^{\circ}C$ and 6.0 to 7.0, respectively. in addition, the best media for growth of that in plate was MCM (Mushroom Complete Media) which have a growth length from 68 to 83 mm. In vivo test, we observed that fast growth and good density of hyphae in mixture media of douglas fir sawdust, cotton seed meal and beet pulp (6:2:2 V/V). Also when we cultivated F. velutipes in this media, we harvested high yield of fruiting body.

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Cyclic Lond Testing for Strong Axis Joints Connected with SRC Column and RC Beams (SRC기둥-RC보 강축 접합부 상세의 구조성능 평가)

  • Moon, Jeong-Ho;Lee, Kang-Min;Lim, Jae-Hyung;Oh, Kyung-Hwan;Kim, Sung-Ho
    • Journal of the Korea Concrete Institute
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    • v.19 no.4
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    • pp.401-409
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    • 2007
  • The objective of this research is to provide better knowledge on the behavior of strong axis SRC column-RC beam joint, supported by experimental results, that can be broadly applicable to many structures. For this purpose, firstly literature reviews and field survey were made to classify the most commonly used for these types of joints. Then, experimental program was designed and performed including 6 SRC column-RC beam joint specimens designed with various joint details. Using the experimental results obtained from the quasi-static cyclic tests, structural performances of the joints such as hysteretic curves, maximum strength capacities, strength degradation beyond the maximum strength, ductilities, and energy dissipation capacities were investigated. Test results showed that specimens with wide beam shape (RCW-P, RCW-W, RCW-F) and T beam shape (RCT-W) showed better structural performances than the bracket type specimens (HBR-L, HBR-S). These specimens also revealed to have higher strength capacities than the nominal design strength. However, H beam bracket type specimens (HBR-L, HBR-S) need further study both analytically and experimentally to verify the reason for unexpected structural performances.