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Angioimmunoblastic T-Cell Lymphoma with Polyclonal Proliferation of Plasma Cells: A Cautionary Note for Flow Cytometry Interpretations (유세포 분석의 주의사항: 혈관면역모세포성 T세포 림프종에서 관찰된 다클론성 형질세포)

  • Shin, Woo Yong;Bang, Hae In;Kim, Jung-Ah;Kim, Jieun;Park, Rojin
    • Korean Journal of Clinical Laboratory Science
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    • v.54 no.1
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    • pp.68-72
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    • 2022
  • Angioimmunoblastic T-cell lymphoma (AITL) is a lymphoproliferative disorder of mature T follicular helper cells. Atypical lymphoid cells were observed in the bone marrow of an 80-year-old woman, and the flow cytometric determined immunophenotypes of B-cells were unusual, that is, CD19+, CD20-, and CD22- with lambda light chain restriction. Initially, we suspected BM involvement of B-cell lymphoma based on the presence of abnormal B-cells. However, the patient was diagnosed with AITL involving BM. A re-analysis of flow cytometric immunophenotyping revealed a minor, aberrant T-cell population, and the lambda light chain restriction observed by surface staining was considered non-specific binding. This case demonstrates B-cells in patients with EBV-positive T-cell lymphoma may exhibit immunophenotypes resembling those of plasma cells, and that proliferation of abnormal B-cells or plasma cells could also potentially mask underlying T-cell lymphoma. A more integrated approach is required for accurate diagnosis.

Suppressive Effects on Lipid Accumulation and Expression of Interleukin-1β-Mediated Inducible Nitric Oxide Synthase in 3T3-L1 Preadipocytes by a Standardized Commercial Noni Fruit Juice (Noni Fruit Juice의 3T3-L1 지방전구세포 분화 억제 및 인터루킨-1β 유도 Inducible Nitric Oxide Synthase 염증유전자 발현 감소 효과)

  • Byeong-Churl Jang
    • Journal of Korean Medicine for Obesity Research
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    • v.23 no.1
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    • pp.1-9
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    • 2023
  • Objectives: Noni fruit juice (NFJ) is liquor extracted from Morinda citrifolia (noni) fruit and has been used as an herbal remedy in many countries. However, the NFJ's anti-adipogenic and anti-inflammatory effects on adipocytes are poorly understood. The purpose of this study was to explore the commercially standardized NFJ effects on lipid accumulation throughout 3T3-L1 preadipocytes differentiation and interleukin-1β (IL-1β)-mediated inducible nitric oxide synthase (iNOS) expression in 3T3-L1 preadipocytes. Methods: Cellular lipid accumulation and triglyceride (TG) content in differentiating 3T3-L1 preadipocytes were assessed subsequently via the Oil Red O staining and AdipoRed assay. MTS assay was used to examine NFJ cytotoxicity in (differentiating) 3T3-L1 preadipocytes. Immunoblotting and reverse transcriptase polymerase chain reaction analysis were used to measure the expression levels of target protein and mRNA in (differentiating) 3T3-L1 preadipocytes, respectively. Results: NFJ treatment at 150 μL/mL led to a substantial reduction of fat accumulation and TG content during 3T3-L1 adipogenesis with no discernable impact on the cell viability. Of note, while NFJ treatment (150 μL/mL) largely inhibited the CCAAT/enhancer-binding protein-α (C/EBP-α) and peroxisome proliferator-activated receptor-β (PPAR-β) protein expressions, it did not influence PPAR-γ in differentiating 3T3-L1 preadipocytes. Of interest, treatment with IL-1β at 20 ng/mL for 4 hours elicited in firm induction of iNOS mRNA expression in 3T3-L1 preadipocytes. However, NFJ treatment at 100 or 200 μL/mL greatly attenuated the IL-1β-induced iNOS mRNA expression in 3T3-L1 preadipocytes. Conclusions: NFJ has anti-adipogenic and anti-inflammatory effects on (differentiating) 3T3-L1 preadipocytes which are in part intervened via control of the expression of C/EBP-α, PPAR-β, and iNOS.

Prevalence of Trichomonas vaginalis in Women Visiting 2 Obstetrics and Gynecology Clinics in Daegu, South Korea

  • Goo, Youn-Kyoung;Shin, Won-Sik;Yang, Hye-Won;Joo, So-Young;Song, Su-Min;Ryu, Jae-Sook;Lee, Won-Myung;Kong, Hyun-Hee;Lee, Won-Ki;Lee, Sang-Eun;Lee, Won-Ja;Chung, Dong-Il;Hong, Yeonchul
    • Parasites, Hosts and Diseases
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    • v.54 no.1
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    • pp.75-80
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    • 2016
  • This study explored epidemiological trends in trichomoniasis in Daegu, South Korea. Wet mount microscopy, PCR, and multiplex PCR were used to test for Trichomonas vaginalis in vaginal swab samples obtained from 621 women visiting 2 clinics in Daegu. Of the 621 women tested, microscopy detected T. vaginalis in 4 (0.6%) patients, PCR detected T. vaginalis in 19 (3.0%) patients, and multiplex PCR detected T. vaginalis in 12 (1.9%) patients. Testing via PCR demonstrated high sensitivity and high negative predictive value for T. vaginalis. Among the 19 women who tested positive for T. vaginalis according to PCR, 94.7% (18/19) reported vaginal signs and symptoms. Notably, more than 50% of T. vaginalis infections occurred in females younger than 30 years old, and 58% were unmarried. Multiplex PCR, which simultaneously detects pathogens from various sexually transmitted infections, revealed that 91.7% (11/12) of patients were infected with 2 or more pathogens. Mycoplasma hominis was the most prevalent co-infection pathogen with T. vaginalis, followed by Ureaplasma urealyticum and Chlamydia trachomatis. Our results indicate that PCR and multiplex PCR are the most sensitive tools for T. vaginalis diagnosis, rather than microscopy which has been routinely used to detect T. vaginalis infections in South Korea. Therefore, clinicians should take note of the high prevalence of T. vaginalis infections among adolescent and young women in order to prevent persistent infection and transmission of this disease.

A Study on the Consumption of Korean Traditional Rice Cakes by College Students (전통 떡류에 대한 대학생들의 이용 현황에 관한 연구)

  • 정효선;서경화;신민자
    • Korean journal of food and cookery science
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    • v.20 no.1
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    • pp.26-33
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    • 2004
  • The purpose of this study was to investigate the consumption patterns of traditional Korean rice cake among college students. Self administered questionnaires were collected from 512 college students in the Seoul, Kyunggi, Chungchung, Kyungsang, Junla and Gangwondo areas. The data were statistically analysed using frequency analysis, chi-squared and t-tests and a one-way ANOVA. By examining the results of the student's perceptions of traditional Korean rice cake, it was found that most know little, or only a moderate amount, about this type of food. Meanwhile, more than half the students liked traditional Korean rice cake, as this type of food satisfied their need for a traditional Korean taste, while the reason for disliking was that they were more familiar with western-style confectionary. Most students answered that they only ate traditional Korean rice cake on special occasions, such as big holidays or ceremonies for the dead ancestors. Most answered that they bought those foods at conventional markets, the mile in the neighborhood, (Eds note: I dont't understand, do you mean, “within a mile of their neighborhood”\ulcorner) or atbig malls. The factor they considered the most important at the time of purchase was the quality (taste), which demonstrates the need for the development of traditional Korean rice cakes, with new tastes and shapes, which still satisfy the Korean's taste, whilemaintaining the traditional taste. For the questions that asked about the problems faced by the traditional Korean rice cake manufacturing industry, and the reasons for low consumption, the students responded that buying this type of food was difficult as the outlets selling themwere not easy to find. Our results seem to suggest that there are almost no specialty stores for the sale of traditional Korean rice cake compared to the other types of cakes that are scattered all over the country. (Eds note: this is only my opinion, and I maybe wrong, but I would have thought that trying to market traditional Korean rice cakes at local convenience stores, rather than speciality stores, would make them more available to the general public. Their marketing at speciality stores will maintain the status quo, i.e. people will go to the speciality stores to purchase product for holidays and special occasions, whereas they will go to the local convenience stores to buy general everyday snacks etc.)

Effect of pH Control on Physico-chemical Characteristics of Chicken Breast Surimi (닭가슴살 Surimi의 이화학적 특성에 미치는 pH 조절의 영향)

  • Jin Sang-Keun;Kim Il-Suk;Hur Sun-Jin;Park Ki-Hun;Ha Ji-Hee;Kang Seoc-Mo;Choi Yeung-Joon;Kim Jin-Soo
    • Food Science of Animal Resources
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    • v.26 no.1
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    • pp.64-69
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    • 2006
  • This study was conducted to determine the effect of pH adjustment on physico-chemical characteristics of chicken breast surimi. The chicken breast meat was ground with distilled water, of which pH was then adjusted to 2.5 (T1), 3.0 (T2), 10.5 (T3) and 11.0 (T4) for surimi manufacture, respectively. Water content was higher in order of T4>T1>T3>T2 (p<0.05). Crude protein and crude fat were higher in T3 and T4 compared with T1 and T2 (p<0.05). $L^*$ values, myofibrillar protein and water holding capacity of T2 and T4 were higher than those of T1 and T3 (p<0.05). T4 had the lowest yield among the treatments (p<0.05). T1 was higher in yield and pH, whereas breaking force and deformation were higher in T1 (p<0.05). $a^*$ was higher in order of T3>T2>T4>T1 and $b^*$ was lower in T1 compared with other treatments (p<0.05). In textural properties, the chewiness values of T2 and T3 were higher than those of T1 and T4, the hardness was higher in order of T2>T3>T4>T1 (p<0.05). Cohesiveness and gumminess of T1 showed higher values than those of other treatments (p<0.05). In sensory evaluation, the note for appearance was higher in T2 than other treatments (p<0.05), however other traits were not significantly different (p>0.05). Therefore, the alkaline processing (T4, pH 11.0) would be recommended.

Epidemiological Note on the Clonorchiasis in Samrangjin Eup, Milyang Gun, Kyongsang Nam Do (=province) (경상남도(慶尙南道) 밀양군(密陽郡) 삼랑진읍(三浪津邑)에 있어서의 간흡충(肝吸虫) 감염실태조사(感染實態調査))

  • Rim, Han-Jong;Joo, Kyoung-Hwan;Eom, Kee-Seon;Park, Soo-Bae
    • Journal of agricultural medicine and community health
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    • v.7 no.1
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    • pp.80-89
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    • 1982
  • In order to observe the endemic status of Clonrchis sinensis infection in Samrangjin eup, Milyang gun, Kyongsang nam do, a total number of 294 stool specimens were collected from the inhabitants who lived in the basin of the Nakdong river. The specimens were examined by cellophane thick smear technique and Stoll's egg dilution technique during the period from May to June 1983. The epidemiological status was analysed statistically by the regression equation and catalytic curv with the results obtained from this area. The results are as follows : 1) The infestation rate of Clonorchis sinensis in 294 was 49.0%. 2) No remarkable difference was observed between male (52.5%) and female (44.8%). 3) Average E.P.G. by Stoll's egg dilution technique in this area was 9,597. 4) The degree of the intensity of Clonorchis infection by E.P.G. count was distributed as 10.5% in very heavy infection, 14.7% in heavy infection, 47.4% in moderate infection, and 29.3% in light infection. 5) The intensity of endemicity in this area was represented with the regression equation calculated with the cumulative percentages of E.P.G. counts. Regression equation was y=3.40+1.23 log x and Cs. $D._{50}$ was 19.99. 6) The two stage catalytic model was applied and the calculation lead to the equation $y=1296(e^{-0.008t}-e^{-0.035t})$ ; a=0.035 > b=0.008 in this surveyed area. 7) Other helminthic infection rate in this area was 7.1% in A. lumbricoides, 9.5% in T. trichiura and 4.1% in E. vermicularis respectively.

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Study on the Dispute for the Dominium of Diàoyútái(Senkaku Islands) and Legal Principles between the Countries Concerned (조어대(센카쿠열도)의 영유권 분쟁과 당사국간 법리에 관한 연구)

  • Yang, Hee Cheol;Kim, Jin Wook
    • Ocean and Polar Research
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    • v.36 no.3
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    • pp.255-276
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    • 2014
  • The dispute between China and Japan regarding $Di\grave{a}oy\acute{u}t\acute{a}i$ is believed to be quite similar to the controversy surrounding Dokdo in terms of historical and post-war processes except for the point that the phenomena of occupation is different with regard to Dokdo. China's claim to $Di\grave{a}oy\acute{u}t\acute{a}i$ is based on historical title and continuous use while the basis of Japan's claim is summarized as preoccupancy of ownerless land. Even though Japan acknowledges that China discovered $Di\grave{a}oy\acute{u}t\acute{a}i$, Japan claims that the act to establish sovereignty over the island from the standpoint of International Law was not taken by China. However, at that time, effective occupation was not an essential prerequisite for the acquisition of a territory. That is to say, from a legal perspective, the legal right for an area could be established based on the discovery of the land, and so it is thought that Japan is applying the current criteria of International Law in a manner that is inappropriate. When we review the post-war process, the San Francisco Peace Treaty does not directly mention $Di\grave{a}oy\acute{u}t\acute{a}i$. But based on the said treaty, we can note that Japan gave up all rights for the southern area that is north of the boundary line that equates to latitude $29^{\circ}$ and that includes the Ryuku Islands and $Di\grave{a}oy\acute{u}t\acute{a}i$. Of course, the provisions for the territory in the San Francisco Peace Treaty and its disposal are not the final factor for the judgment regarding dominium of $Di\grave{a}oy\acute{u}t\acute{a}i$. However, it seems clear that Japan's attitude and interpretation regarding the issues of $Di\grave{a}oy\acute{u}t\acute{a}i$, the Kuril Islands and Dokdo is problematic.

A Study on Musical Home Environment and Children's Musical Development (가정의 물리적, 인적 음악 환경과 아동의 음악성 발달에 관한 연구)

  • 김명순;이소희
    • Journal of the Korean Home Economics Association
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    • v.37 no.7
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    • pp.83-94
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    • 1999
  • The purpose of this study was to explore musical development of 3- to S-year-old children and their musical home environment. The subjects were one hundred ninety-four children and their mothers enrolled in four kindergartens in Seoul. Each child sang the birthday song with peers in a birthday play setting. It was audiotaped for the children to sing the song. Questionnaire of musical home environment developed by the researchers was used for the mothers. The children's rhythm and pitch development were coded by the scoring categories of Project Spectrum(Krechevsky, 1994). The data were analyzed by t-test, ANOVA, Scheffe, and Pearson correlation. The results of this study were as follows: Firstly, there was no a significant difference in the children's rhythm development among three age-groups as well as between boys and girls. Among rhythm subcategories, the unit of note was ranked in the highest score and the pulse the next. Secondly, there were significant differences in children's pitch development among three age-groups and between boys and girls. The older children significantly achieved higher scores than the younger. Among pitch subcategories, the contour was ranked in the highest score and the interval the next. Thirdly, the children's musical development and their physical home environment related to music were correlated positively. The children's pitch development was significantly related to the mothers' musical attitude and the children's rhythm development to the mothers' educational levels.

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Comparison of Cancer Nursing. Interventions Recorded in Nursing Notes with Nursing Interventions Perceived by Nurses of an Oncology Unit - Patients with Terminal Cancer - (간호일지 상의 간호중재와 지각된 간호중재의 수행빈도 비교 -말기 암환자를 중심으로-)

  • Chai Ja-Yun;Jang Keum-Seang
    • Journal of Korean Academy of Nursing
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    • v.35 no.3
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    • pp.441-450
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    • 2005
  • Purpose: The purposes of this study were to determine the core nursing interventions in nursing notes and the practice which was perceived by nurses of an oncology unit with patients with terminal cancer. Also, comparing interventions in nursing notes with interventions in perceived practice was done. Method: Subjects were 44 nursing records of patients with terminal cancer who had died from Jan. to Dec. 2002 at C University Hospital and 83 nurses who were working on an oncology unit for more than one year. Data was collected using a Nursing Interventions Classification and analyzed by means of mean and t-test. Results: The most frequent nursing intervention was 'nausea management' in the nursing note and was 'medication administration: oral' in perceived practice. The frequency of nursing interventions in the nursing record was lower than in perceived practice. Conclusion: This study finds that nurses actually practice nursing care, but they may omit records. To correct for omitted nursing records, development of a systematic nursing record system, continuous education and feedback is recommended.

The Effect of Dramatic Play Using Economic-Fairy Tales on Preschoolers' Basic-Economic Understanding and Consumer Behavior (경제동화를 활용한 극놀이 활동이 유아의 경제개념 및 소비자행동에 미치는 영향)

  • Chae, Young-Ran
    • Korean Journal of Human Ecology
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    • v.19 no.3
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    • pp.443-453
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    • 2010
  • This study analyzed the effects of dramatic play using Economic-fairy tales on young children's basic- economic understanding and the consumer behavior. The participants in this study were 42 young children who attended a kindergarten in G city. The mean age of participants was 70 months. The experimental group participated in 'Dramatic play using Economic-fairy tales' while the control group participated in a 'Discussion activity using Economic-fairy tales'. The experiment used 'The Basic-Economic Concept Test' and 'The Consumer Behavior Test' in the collection of data and T-test was used to statistically analyze the data. Significant differences were found between the experimental group and comparison group in both the post-test score of Basic-Economic Understanding and of Consumer Behavior NOTE. In conclusion, the results of this study suggested that the technique of dramatic play using Economic-fairy tales which was developed in this study are probably effective in developing young children's basic-economic understanding and consumer behavior.