• 제목/요약/키워드: Synthetic medium

검색결과 366건 처리시간 0.033초

Effects of Some Amino Acids on Ammonia Secretion and Extracellular Protease Activity by Three Oomycetes in Synthetic Medium with or without Glucose

  • Ali, Esam H.
    • Mycobiology
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    • 제33권1호
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    • pp.23-29
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    • 2005
  • The effects of different concentrations of three amino acids as carbon and or nitrogen sources on mycelial dry weights, changes in pH values of synthetic medium, ammonia secretion and extracellular protease activity by three zoosporic fungi, pathogens of fish and shellfish, were studied. As compared with the control, the addition of isoleucine and aspartic acid as nitrogen sources were generally stimulative for mycelial dry weight production whereas phenylalanine was inhibitory irrespective to the tested fungal species. When amino acids served as carbon and nitrogen sources, the mycelial dry weights of the three fungi were increased (mostly non-significantly) relative to untreated control but weights were decreased as the concentrations of the three amino acids raised. The addition of individual amino acids as carbon and nitrogen sources to the medium significantly increased pH values of the medium comparable to the control. The addition of each of the three amino acids as carbon and nitrogen sources to the medium significantly induced ammonia secretion by the three species of zoosporic fungi. Ammonia secretion in synthetic medium amended with amino acids as nitrogen source raised by the three zoosporic fungi relative to untreated control except in case of Achlya racemosa treated with isoleucine. Extracellular protease activity was almost promoted in case of Achlya proliferoides and Saprolegnia furcata cultures treated with isoleucine and aspartic acid individually in presence of glucose and vice versa in case of phenylalanine. However, extracellular protease activity of A. racemosa decreased compared with the control at various concentrations of isoleucine and both phenylalanine and aspartic acid assumed inconsistent effects. Extracellular protease activity of the three zoosporic fungi in the medium devoid of glucose varied depending upon zoosporic fungal species, the tested amino acid and the applied concentrations. The values of protease activity were approximately less two folds than that obtained in presence of glucose.

어독성 실험에 따른 방근재 친환경 특성 분석 (Analyses on Environment-friendliness of Root Barrier Materials based on Fish Toxicity Test)

  • 우지근;김성균
    • 한국환경복원기술학회지
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    • 제13권4호
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    • pp.42-51
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    • 2010
  • The purpose of this study is to analyze the characteristics of environment-friendliness of Root Barrier materials based on comprehensive experiments on harmfulness of Root Barrier materials and fish toxicity of Oryzias latipes mortality to verify eco-toxicity of each method of construction and Root Barrier material, which are to be applied by taking eco-toxicity into account when building ecological flows in upper areas on natural and artificial grounds. As a result, the following conclusions have been reached in this study: 1. In regard of the harmfulness analyzed, each material showed a different result of analytical value in each lab tank. Compared to lab tank, pH (la), DO (lb), T-N (VI) and T-coli (la) were in the same grade, but COD, SS, T-P and F-coli were less than that of control, respectively increased or decreased by material were analyzed. 2. In the experiment of fish toxicity, Barrier sheet was found to have 66.7% of fish mortality, indicating strong fish toxicity. Synthetic rubber system root barrier sheet (20.0%) was analyzed to have medium fish toxicity, while Synthetic resin system root barrier-waterproof sheet (3.3%), Synthetic rubber system membrane root barrier sheet (3.3%) and Synthetic resin system root barrier sheet (0.0%) showed relatively lower mortality and fish toxicity. To sum up such results as found in the experiment mentioned so far, the values of harmfulness and root penetration resistance analyzed were different in each lab tank, but there was absolutely little correlation with the mortality gained from the experiment on fish toxicity. In the experiment of fish toxicity, environment-friendly root barrier materials were analyzed, and it was found that Synthetic resin system root barrier sheet, Synthetic resin system root barrier waterproof sheet and Synthetic rubber system membrane root barrier sheet are highly environment-friendly. In contrast, Synthetic rubber system root barrier sheet was found to have medium-level environment-friendliness. Also, Barrier sheet was analyzed to have low environment-friendliness.

직류전원을 이용한 배전급 차단기의 등가 3상 합성투입시험법 (Equivalent three-phase synthetic making test for medium voltage circuit breaker of distribution system using DC power)

  • 박병락;조만영;김진석;신희상;김재철
    • 조명전기설비학회논문지
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    • 제25권7호
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    • pp.105-113
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    • 2011
  • The study about three-phase synthetic making test using DC power has been performed in order to increase the making test capacity on Vacuum Circuit Breaker. And, it made possible to solve the limitations that short-circuit testing facilities can not fulfill the testing requirements of VCB exceeding three-phase 36[kV] 31.5[kA]. By using DC power and high speed spark-gap switch, this method made the equivalence with the pre-arc that occurred during the making process under the fault condition of power system. As results, KERI(Korea Electrotechnology Research Institute) could have capacity to carry out type test for VCB under three-phase 52[kV] 40[kV], which satisfies the IEC Standard.

Evaluating the Mechanical Properties of Fiber Yarns for Developing Synthetic Fiber Chains

  • Kim, Kyeongsoo;Kim, Taewan;Kim, Namhun;Kim, Dokyoun;Kang, Yongjun;Kim, Seonjin
    • 한국해양공학회지
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    • 제35권6호
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    • pp.426-433
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    • 2021
  • In this study, three types of synthetic fiber materials were evaluated, namely, DM20, SK78, and T147, to replace steel chains in shipbuilding and offshore fields with fiber chains as there is increasing demand for chains with lighter weights and improved usabilities. The strength and quasi-static stiffness were analyzed to select suitable yarns for the fiber chains. The durability of the yarn was evaluated by performing a 3-T (time to rupture) test as a specific tension level. The results of the experiment revealed excellent dynamic stiffness in DM20 and highest values of the windward and leeward stiffness in T147. 3-T linear design characteristic curves for a specific tension level were derived for the three types of fiber materials. The findings of this study can provide insights for improving strength and durability in fiber chain design.

Evaluation of Field Applicability of Phosphorus Removal Capability and Growth of Bacillus sp. 3434 BRRJ According to Environmental Factors

  • Yoo, Jin;Kim, Deok-Hyun;Chung, Keun-Yook
    • 한국토양비료학회지
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    • 제49권1호
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    • pp.87-92
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    • 2016
  • With the population growth and industrialization, the characteristics of discharged waste water and sewage have become more diverse. The removal of phosphorus (P) in the wastewater is essential for the prevention of eutrophication in the river and stream. This study was performed in order to estimate the field application of the Bacillus sp. 3434 BRRJ. Bacillus sp. 3434 BRRJ was cultured in the raw wastewater and synthetic medium at the 5 L reactor. The best optimum conditions for P removal by Bacillus sp. 3434BRRJ in the synthetic medium at the 5 L reactor were as follows: temperature, $30^{\circ}C$; P concentration, 20 mg/L; carbon sources, glucose + acetate (1:1); oxygen concentration, alternatively anaerobic and aerobic conditions. P removal efficiency under the optimum condition was 89.4%. In case of wastewater, P removal efficiency was 95.5% under controlled at $30^{\circ}C$. Through this study we confirmed that P removal by Bacillus sp. 3434BRRJ in case of wastewater was as effective as the synthetic medium. It is considered that Bacillus sp. 3434 BRRJ can be applied to the treatment of wastewater in order to biologically remove P from the wastewater on a large scale.

중발포 소화약제 소화성능 분석을 통한 중발포기 적용에 관한 연구 (A Study on the Application Medium Expansion Foam Extinguisher by a Fire Performance Analysis used Medium Expention Foam Agent)

  • 이장원
    • 한국재난정보학회 논문집
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    • 제15권3호
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    • pp.402-408
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    • 2019
  • 연구목적 : 포소화설비에 사용되는 중발포 소화약제는 기류에 영향이 적어 방출거리를 유지 할 수 있을뿐만 아니라 소화약제도 효율적으로 사용 할 수 있기 때문에 중발포기를 이용하여 발포배율 및 소화성능을 실험으로 밝히고자 한다. 연구방법 : 본 연구를 위해 중발포 시험장치를 제작하여, 합성계면활성 제포를 대상으 로 유류화재(B급) 소화성능 및 발포배율 분석과 포소화약제 소모량을 측정하고 분석하였다. 연구결과: 중발포기로 발포배율을 측정한 결과 팽창율이 26.1배의 중발포 배율로 나타났으며, 유류화재 20단위 소화모형 실험결과 소화시간이 고발포 및 저발포 보다 빠르게 나타나, 수동식설비인 호스릴 또는 중발포기 적용이 가능할 것으로 본다. 결론: 본 연구를 통해 중발포 포소화약제 발포성능 및 소화성능이 우수하게 나타나는 점을 고려하여 중발포 소화약제를 활용한 호스릴 또는 중발포기의 기술기준 도입이 필요하다.

A study on development of simulation model of Underwater Acoustic Imaging (UAI) system with the inclusion of underwater propagation medium and stepped frequency beam-steering acoustic array

  • L.S. Praveen;Govind R. Kadambi;S. Malathi;Preetham Shankpal
    • Ocean Systems Engineering
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    • 제13권2호
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    • pp.195-224
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    • 2023
  • This paper proposes a method for the acoustic imaging wherein the traditional requirement of the relative movement between the transmitter and target is overcome. This is facilitated through the beamforming acoustic array in the transmitter, in which the target is illuminated by the array at various azimuth and elevation angles without the physical movement of the acoustic array. The concept of beam steering of the acoustic array facilitates the formation of the beam at desired angular positions of azimuth and elevation angles. This paper substantiates that the combination of illumination of the target from different azimuth and elevation angles with respect to the transmitter (through the beam steering of beam forming acoustic array) and the beam steering at multiple frequencies (through SF) results in enhanced reconstruction of images of the target in the underwater scenario. This paper also demonstrates the possibility of reconstruction of the image of a target in underwater without invoking the traditional algorithms of Digital Image Processing (DIP). This paper comprehensively and succinctly presents all the empirical formulae required for modelling the acoustic medium and the target to facilitate the reader with a comprehensive summary document incorporating the various parameters of multi-disciplinary nature.

Micro-propagation Factors Essential for Mass Production of Synthetic Seeds in Banana

  • Hassanein A. M.;Ibrahiem I. A.;Galal A. A.;Salem J. M. M.
    • Journal of Plant Biotechnology
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    • 제7권3호
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    • pp.175-181
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    • 2005
  • This work described some essential factors necessary for micro-propagation of banana for mass production of synthetic seeds for germ plasm conservation, and how peroxides activity of conserved tissue was influenced. Shoot tips of field grown plants were used to obtain shoot clusters on shoot proliferation medium (MS medium supplemented with 5 mg/l BAP). Using longitudinally-split shoot tip technique, 18720, 8640, 7488, 2016 plantlets were obtained from one shoot tip of Maghraby, Grand Naine, Balady, and Williams, respectively, in six subculture, one month each, on solid medium. Shoot tips excised from in vitro grown plantlets were encapsulated in calcium-alginate beads and stored at $4^{\circ}C$ for one month on half-strength MS basal medium without growth regulators or sugars. After one month all the viable-conserved synseeds formed shoots when they were transferred to MS basal medium, some of them showed synchronous formation of shoot and root systems in one week. Plants retrieved from encapsulated shoot tips were hardened off and transferred to soil.

미생물(微生物) Inulase에 관한 연구(硏究) 제1보(第一報) -Inulase생산균주(生産菌株)의 분리(分離)와 효소생산(酵素生産)을 위한 배양조건(培養條件)의 검토(檢討)- (Studies on Microbial Inulase (Part I) -A Study on the Isolation of an Inulase Producing strain and the Optimum Cultural Conditions for the Enzyme Production-)

  • 김기철
    • Applied Biological Chemistry
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    • 제18권1호
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    • pp.42-51
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    • 1975
  • 돼지감자 시료(試料)(Putrefactived and dry Jerusalem artichoke)로 부터 강력(强力)한 Inulin가수분해(加水分解) 효소(酵素)를 생산(生産)하는 Penicillium sp. I을 분리선발(分離選拔)하였고 본균(本菌)을 사용(使用)하여 효소생산(酵素生産)에 관(關)한 최적배양조건(最適培養條件)을 검토(檢討)하여 다음과 같은 결론(結論)을 얻었다. 1. Penicillium sp. I은 Inulase생산력(生産力)이 강(强)한 균주(菌株)이다. 2. 본균(本菌)의 Inulase생산(生産)을 위한 최적배양(最適培養) 조건(條件)을 Jerusalem artichokoke extract media와 Basal media의 두 배양기(培養基)에서 결정(決定) 하였다. 3. Jerusalem artichoke extract media에서 Basal media보다 Inulase생산력(生産力)이 높았다. 4. Inulase생산능(生産能)도 Jerusalem artichoke extract media에서는 4일(日) Basal media에서는 5일(日) 배양(培養)으로써 최고치(最高値)에 도달하였다. 5. Inulin농도(濃度), pH. 온도조건(溫度條件) Jerusalem artichoke extract media에서는 opt. temp $30^{\circ}COpt.$ pH 5.0, Opt. conc 4.0%(W/V)로 나타나고 Basal media에서는 Opt temp $30{\sim}33^{\circ}C$, Opt. pH $5.0{\sim}6.0$, Opt conc $1.0{\sim}1.5%(W/V)$ 6. 질소원(窒素源)으로서는 Corn Steep Liquir, Peptone, $(NH_4)_2HPO_4,\;NH_4H_2PO_4,\;(NH_4)_2SO_4$등이 량호(良好)하고 특히 유기질소원(有機窒素源)을 가(加)함으로써 Basal meaia에서 Inulase생산(生産)을 증가(增加)시켰다. 7. 당원(糖源)의 이용(利用)으로써는 Inulin이외(以外)의 제당(諸糖)은 Inulase생산(生産)에 영향(影響)을 미치지 않았다. 8. 금속(金屬) 염류(鹽類)의 이용(利用)은 KCI. $MgSO_4,\;FeSO_4$등이 Inulase생산(生産)을 증가(增加)시켰다.

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3D SIMULATIONS OF RADIO GALAXY EVOLUTION IN CLUSTER MEDIA

  • O'NEILL SEAN M.;SHEARER PAUL;TREGILLIS IAN L.;JONES THOMAS W.;RYU DONGSU
    • 천문학회지
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    • 제37권5호
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    • pp.605-609
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    • 2004
  • We present a set of high-resolution 3D MHD simulations exploring the evolution of light, supersonic jets in cluster environments. We model sets of high- and low-Mach jets entering both uniform surroundings and King-type atmospheres and propagating distances more than 100 times the initial jet radius. Through complimentary analyses of synthetic observations and energy flow, we explore the detailed interactions between these jets and their environments. We find that jet cocoon morphology is strongly influenced by the structure of the ambient medium. Jets moving into uniform atmospheres have more pronounced backflow than their non-uniform counterparts, and this difference is clearly reflected by morphological differences in the synthetic observations. Additionally, synthetic observations illustrate differences in the appearances of terminal hotspots and the x-ray and radio correlations between the high- and low-Mach runs. Exploration of energy flow in these systems illustrates the general conversion of kinetic to thermal and magnetic energy in all of our simulations. Specifically, we examine conversion of energy type and the spatial transport of energy to the ambient medium. Determination of the evolution of the energy distribution in these objects will enhance our understanding of the role of AGN feedback in cluster environments.