Studies on Microbial Inulase (Part I) -A Study on the Isolation of an Inulase Producing strain and the Optimum Cultural Conditions for the Enzyme Production-

미생물(微生物) Inulase에 관한 연구(硏究) 제1보(第一報) -Inulase생산균주(生産菌株)의 분리(分離)와 효소생산(酵素生産)을 위한 배양조건(培養條件)의 검토(檢討)-

Penicillium sp I which produces a powerful hydrolysing enzyme was isolated from putrefid and dry Jerusalem artichoke medium. The strain was used to study on the optimum culture conditions for enzyme production. The results obtained are as follows: 1. Penicillium sp I was a vigorous strain to produce inulase. 2. The optimum culture conditions of the strain was examined in the Jerusalem artichoke extract medium and the synthetic medium. 3. Inulase productivity in the Jerusalem artichoke extract medium was higher than that of the synthetic medium. 4. The optimum culture period of the Jerusalem artichoke extract medium was four days, whereas that of the synthetic medium was five days. 5. The optimum temperature, pH and concentration in the Jerusalem artichoke extract medium were $30^{\circ}C$, 5.0 and 4.0% (W/V), respectively. Meanwhile, the optimum temperature, pH and concentration in the synthetic medium were $30{\sim}33^{\circ}C$, $5.0{\sim}6.0$, and $1.0{\sim}1.5%$ (W/V), respectively. 6. Corn steep liquor, peptone, $(NH_4)_2HPO_4,\;NH_4H_2PO_4,\;(NH_4)_2SO_4$, etc. were favorable as nitrogen sources. Of these, especially, Corn steep liquor and peptone as organic nitrogen sources caused an increase in inulase production in the synthetic medium. 7. All sugars except for inulin have no effect upon the inulase production. 8. KCl, $MgSO_4\;and\;FeSO_4$ were favourable mineral sources for inulase production.

돼지감자 시료(試料)(Putrefactived and dry Jerusalem artichoke)로 부터 강력(强力)한 Inulin가수분해(加水分解) 효소(酵素)를 생산(生産)하는 Penicillium sp. I을 분리선발(分離選拔)하였고 본균(本菌)을 사용(使用)하여 효소생산(酵素生産)에 관(關)한 최적배양조건(最適培養條件)을 검토(檢討)하여 다음과 같은 결론(結論)을 얻었다. 1. Penicillium sp. I은 Inulase생산력(生産力)이 강(强)한 균주(菌株)이다. 2. 본균(本菌)의 Inulase생산(生産)을 위한 최적배양(最適培養) 조건(條件)을 Jerusalem artichokoke extract media와 Basal media의 두 배양기(培養基)에서 결정(決定) 하였다. 3. Jerusalem artichoke extract media에서 Basal media보다 Inulase생산력(生産力)이 높았다. 4. Inulase생산능(生産能)도 Jerusalem artichoke extract media에서는 4일(日) Basal media에서는 5일(日) 배양(培養)으로써 최고치(最高値)에 도달하였다. 5. Inulin농도(濃度), pH. 온도조건(溫度條件) Jerusalem artichoke extract media에서는 opt. temp $30^{\circ}COpt.$ pH 5.0, Opt. conc 4.0%(W/V)로 나타나고 Basal media에서는 Opt temp $30{\sim}33^{\circ}C$, Opt. pH $5.0{\sim}6.0$, Opt conc $1.0{\sim}1.5%(W/V)$ 6. 질소원(窒素源)으로서는 Corn Steep Liquir, Peptone, $(NH_4)_2HPO_4,\;NH_4H_2PO_4,\;(NH_4)_2SO_4$등이 량호(良好)하고 특히 유기질소원(有機窒素源)을 가(加)함으로써 Basal meaia에서 Inulase생산(生産)을 증가(增加)시켰다. 7. 당원(糖源)의 이용(利用)으로써는 Inulin이외(以外)의 제당(諸糖)은 Inulase생산(生産)에 영향(影響)을 미치지 않았다. 8. 금속(金屬) 염류(鹽類)의 이용(利用)은 KCI. $MgSO_4,\;FeSO_4$등이 Inulase생산(生産)을 증가(增加)시켰다.