• Microbiology and Biotechnology Letters
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• v.48 no.1
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• pp.24-31
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• 2020

Five genes (mxbDM, mxcQE and mxaB) are responsible for the transcription of methanol oxidation genes in Methylobacterium strains. Among these, MxbDM and MxcQE constitute the two-component system (TCS) regulating methanol metabolism. In this study, we integrated the methanol-sensing domain of MxbD and MxcQ with the EnvZ/OmpR from Escherichia coli. The domain-swapping strategy resulted in chimeric histidine kinases (HK's) MxbDZ and MxcQZ AM1 containing recombinant E. coli. Real-time quantitative PCR was used to monitor OmpC expression mediated by the chimeric HK and response regulator (RR) OmpR. Further, an ompC promoter based fluorescent biosensor for sensing methanol was developed. GFP fluorescence was studied both qualitatively and quantitatively in response to environmental methanol. GFP measurement also confirmed ompC expression. Maximum fluorescence was observed at 0.05% methanol and 0.01% methanol using MxbDZ and MxcQZ AM1, respectively. Thus the chimeric HK containing E. coli were found to be highly sensitive to methanol, resulting in a rapid response making them an ideal sensor.

• Molecules and Cells
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• v.19 no.2
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• pp.246-255
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• 2005

Retroviral integrases insert viral DNA into target DNA. In this process they recognize their own DNA specifically via functional domains. In order to analyze these functional domains, we constructed six chimeric integrases by swapping domains between HIV-1 and HFV integrases, and two point mutants of HFV integrase. Chimeric integrases with the central domain of HIV-1 integrase had strand transfer and disintegration activities, in agreement with the idea that the central domain determines viral DNA specificity and has catalytic activity. On the other hand, chimeric integrases with the central domain of HFV integrase did not have any enzymatic activity apart from FFH that had weak disintegration activity, suggesting that the central domain of HFV integrase was defective catalytically or structurally. However, these inactive chimeras were efficiently complemented by the point mutants (D164A and E200A) of HFV integrase, indicating that the central domain of HFV integrase possesses potential enzymatic activity but is not able to recognize viral or target DNA without the help of its homologous N-terminal and C-terminal domains.

• Journal of Plant Biotechnology
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• v.4 no.4
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• pp.163-170
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• 2002

Radiation induced and somaclonal variations were investigated in the regenerates from gamma irradiated and controlled embryogenic callus (EC) of sweetpotato cvs., Yulmi and White Star by morphological, RAPD and AFLP analysis. Most (approx. 90%) of the EC produced somatic embryos developed into plantlets after being transferred to the auxin-free medium. The frequency of morphological variants derived from the irradiated callus ranged from 3 to 7.8% compared to 0.1-1.1% of that derived from the non-irradiated. Morphological variants were selected from the regenerates and analyzed by RAPD and AFLP procedures. RAPD polymorphisms of Yulmi and White Star regenerates from irradiated calli were 8.8% and 6.1%, respectively. However, the polymerphisms among regenerates from the non-irradiation treatment in these two cultivars were non-detectable and 3%, respectively. AFLP polymorphisms of Yulmi and White Star regenerates from irradiated calli were 29.9% and 28.6%, respectively. while the frequencies for those form non-irradiated calli were 8.5% and 5.6%, respectively. Both the control plants and variants from the nonirradiated were clustered together, while variants from irradiated were separated from the group by Nearest-Neighbor-Interchange Branch Swapping Abbreviation: EC (Embryogenic callus), AFLP (Amplified Fragment Length Polymorphism), RAPD (Random amplified polymorphic DNA)

• Journal of Computing Science and Engineering
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• v.8 no.3
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• pp.157-172
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• 2014

Contemporary embedded systems often use NAND flash memory instead of hard disks as their swap space of virtual memory. Since the read/write characteristics of NAND flash memory are very different from those of hard disks, an efficient page replacement algorithm is needed for this environment. Our analysis shows that temporal locality is dominant in virtual memory references but that is not the case for write references, when the read and write references are monitored separately. Based on this observation, we present a new page replacement algorithm that uses different strategies for read and write operations in predicting the re-reference likelihood of pages. For read operations, only temporal locality is used; but for write operations, both write frequency and temporal locality are used. The algorithm logically partitions the memory space into read and write areas to keep track of their reference patterns precisely, and then dynamically adjusts their size based on their reference patterns and I/O costs. Without requiring any external parameter to tune, the proposed algorithm outperforms CLOCK, CAR, and CFLRU by 20%-66%. It also supports optimized implementations for virtual memory systems.

• Journal of KIISE:Computing Practices and Letters
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• v.16 no.7
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• pp.753-758
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• 2010

In this paper we propose an efficient marker tracking system that exploits IR and web cameras. The proposed method solves the marker swap problem and allows for fast and responsive marker tracking. We use color information gathered from the IR reflector to assign a unique identification to each marker. We can locate each marker withthe IR camera and also identify the marker uniquely by using color information provided by the web camera. The experiment results show that marker swapping can be eliminated effectively. Furthermore, our approach allows for faster and more responsive marker tracking.

• Phonetics and Speech Sciences
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• v.5 no.4
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• pp.91-98
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• 2013

This study investigates the difference in the evaluation of foreign-accentedness of English pronunciation across subject groups, evaluated accents, and compared components. This study independently evaluates the prosody and segmentals of the foreign-accented English sentences by pairwise difference rating. Using the prosody swapping technique, segmentals and prosody of the English sentences read by native speakers of American English (one male and one female) were combined with the corresponding segmentals and prosody of the English sentences read by male and female native speakers of Chinese, Japanese or Korean (one male and one female from each native language). These stimuli were evaluated by 4 different subject groups: native speakers of American English, Korean, Chinese, and Japanese. The results showed that the Japanese subject group scored higher in prosody difference than in segmental difference while the other groups scored the other way around. This study is significant in that the attitude toward the difference in segmentals and prosody of the foreign accents of English varies with the native language of the subject group. In other words, for native speakers of some languages, the difference in prosody could have a greater influence on the foreign-accentedness than the difference in segmentals, while for native speakers of other languages the other way around.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.44 no.5
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• pp.42-49
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• 2007

This paper introduces a study on reliability-driven device placement using simulated annealing algorithm which can be applicable to MCM or electronic systems embedded in a spacecraft running at thermal conduction environment. Reliability of the unit's has been predicted with the devices' junction temperatures calculated from FDM solver and optimized by simulated annealing algorithm. Simulated annealing in this paper adopts swapping devices method as a perturbation. This paper describes and compares the optimization simulation results with respect to two objective functions: minimization of failure rate and minimization of average junction temperature. Annealing temperature variation simulation case and equilibrium coefficient variation simulation case are also presented at the two respective objective functions. This paper proposes a new approach for reliability optimization of MCM and electronic systems considering those simulation results.

• Journal of Broadcast Engineering
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• v.20 no.4
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• pp.606-618
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• 2015

Computer graphics(CG) is being important technique in media contents such as movie and TV. Especially, face replacement technique which replaces the faces between different images have been studied as a typical technology of CG by academia and researchers for a long time. In this paper, we propose the face replacement method between target and reference images under different illumination environment without 3D model. In experiments, we verified that the proposed method could naturally replace the faces between reference and target images under different illumination condition.

• Human Ecology Research
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• v.55 no.4
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• pp.351-362
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• 2017

Collaborative consumption (CC) occurs in organized systems or networks in which participants conduct sharing activities in the form of renting, lending, trading, bartering, and swapping of goods, services, transportation solutions, space, or money. Information and communications technologies (ICTs) that have emerged with CC. CC is expected to alleviate social problems such as hyper-consumption, pollution, and poverty by lowering the cost of economic coordination. In this study, we investigate the influence of educational experience and expected benefits of CC participation (intended to using and providing CC) of Adolescent Consumers. The subjects for the study were 418 high school students. Data was analyzed through frequency analysis, mean, standard deviation, t-test, ANOVA, Pearson's correlation, and hierarchical multiple regression analysis using SPSS Win 21.0. The results of this study are as follows. First, the significant positive relationship found between CC participants (intent to use and provide CC), educational experience (home education, school education, and mass media) and expected benefits (social benefit, economic benefit, enjoyment, community effect, and reputation). Second, enjoyment, mass media, reputation, social benefit, home education and school education values were variables that influenced the using participation intention for CC. Third, the major variables influencing the providing participation intention CC were home education, enjoyment, gender, community effect, and mass media values.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.1A
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• pp.70-75
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• 2005

The efficient hardware design of the the algorithm is important in wide variety of DSP. One example is OFDM(Orthogonal Frequency Division Multiplexing) based WLAN(Wireless Local Area Network) systems which place high requirements on throughput and power consumption on FFT. The output RAM is composed of two banks of $64{\times}W.$ The banks are swapped immediately following the falling edge or the start signal strobe. This bank swapping allows 64-Point FFT to continue Processing samples and to continue filling the alternative bank, without affecting the data flow outputs.