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Characterization of the Functional Domains of Human Foamy Virus Integrase Using Chimeric Integrases  

Lee, Hak Sung (Department of Biotechnology and Bet Research Institute, Chung-Ang University)
Kang, Seung Yi (Department of Biotechnology and Bet Research Institute, Chung-Ang University)
Shin, Cha-Gyun (Department of Biotechnology and Bet Research Institute, Chung-Ang University)
Publication Information
Molecules and Cells / v.19, no.2, 2005 , pp. 246-255 More about this Journal
Abstract
Retroviral integrases insert viral DNA into target DNA. In this process they recognize their own DNA specifically via functional domains. In order to analyze these functional domains, we constructed six chimeric integrases by swapping domains between HIV-1 and HFV integrases, and two point mutants of HFV integrase. Chimeric integrases with the central domain of HIV-1 integrase had strand transfer and disintegration activities, in agreement with the idea that the central domain determines viral DNA specificity and has catalytic activity. On the other hand, chimeric integrases with the central domain of HFV integrase did not have any enzymatic activity apart from FFH that had weak disintegration activity, suggesting that the central domain of HFV integrase was defective catalytically or structurally. However, these inactive chimeras were efficiently complemented by the point mutants (D164A and E200A) of HFV integrase, indicating that the central domain of HFV integrase possesses potential enzymatic activity but is not able to recognize viral or target DNA without the help of its homologous N-terminal and C-terminal domains.
Keywords
Chimeric Integrase; Defective Point Mutants; Functional Domain;
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Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 7  (Related Records In Web of Science)
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