• 제목/요약/키워드: Surface plasmon resonance(SPR)

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폴리스티렌 나노 비드를 이용한 플라즈모닉 나노 구조체의 광학 특성 (Optical Characteristics of Plasmonic Nano-structure Using Polystyrene Nano-beads)

  • 김두근;정병규;김홍승;김태룡;김선훈;기현철;김태언;신재철;최영완
    • 한국전기전자재료학회논문지
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    • 제28권4호
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    • pp.244-248
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    • 2015
  • We proposed and demonstrated the double layered metallic nano-hole structure using polystyrene beads process to enhance the sensitivity of surface plasmon resonance (SPR). The double layered SPR structures are calculated using the finite-difference time-domain (FDTD) method for the width, thickness, and period of the metallic nano-hole structures. The thickness of the metal film and the metallic nano-hole is 30 and 20 nm in the 214 nm wide nano-hole size, respectively. The double layered SPR structures are fabricated with monolayer polystyrene beads of 420 nm wide. The sensitivities of the conventional SPR sensor and the double layered SPR sensor are obtained to 42.2 and 52.1 degree/RIU, respectively.

Immunoaffinity Characteristics of Exosomes from Breast Cancer Cells Using Surface Plasmon Resonance Spectroscopy

  • Sohn, Young-Soo;Na, Wonhwi;Jang, Dae-Ho
    • 센서학회지
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    • 제28권6호
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    • pp.355-359
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    • 2019
  • Exosomes, known as nanoscale extracellular vesicles in the range of 30-150 nm, are known to contain clinically significant information. However, there is still insufficient information on exosomal membrane proteins for cancer diagnosis. In this work, we investigated the characteristics of the membrane proteins of exosomes shed by cultured breast cancer cell lines using a surface plasmon resonance (SPR) spectroscopy and pre-activated alkanethiols modified sensor chips. The antibodies of breast cancer biomarkers such as MCU-16, EpCAM, CD24, ErbB2, and CA19-9 were immobilized on the pre-activated alkanethiols surfaces without any activation steps. The purified exosomes were loaded onto each antibody surface. The affinity rank of the antibody surfaces was decided by the relative capture efficiency factors for the exosomes. In addition, an antibody with a relative capture efficiency close to 100% was tested with exosome concentration levels of 104/µl, 105/µl, and 106/µl for quantitative analysis.

플라즈모닉스 현상을 이용한 전반사 기반 다층 유전체 박막 센서의 특성 분석 (Characteristics Analysis of Total Internal Reflection-based Dielectric Multi-layer Sensor Using Plasmonics Phenomena)

  • 김홍승;이태경;김두근;정유라;오금윤;이병현;기현철;최영완
    • 한국전기전자재료학회논문지
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    • 제25권7호
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    • pp.516-520
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    • 2012
  • In this paper, we have theoretically analyzed and designed a dielectric multi-layer sensor with a SPR (surface plasmon resonance) using analytical calculation and FDTD (finite difference time-domain) methods. The proposed structure is composed of periodic layer and thin metal film. It has many advantages. One of that is a high sensitivity of the SPR. Another is a high Q-factor of the characteristics in the PhC (photonic crystals) micro-cavity structure. The incident light has double resonance characteristics, because the filtered light by PhC structure, dielectric multi-layer, is met the thin metal film for SPR effect. We have also observed the change of resonance characteristics according to the variation of effective index on the metal film.

금 나노입자의 광열효과를 이용한 온열화장품 개발 (Development of Thermo-Cosmetics Using Photothermal Effect of Gold Nanoparticles)

  • 이재열;김보미;박세호;최요한;심규동;문성배;장의순;양선아;지광환
    • 대한화장품학회지
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    • 제41권1호
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    • pp.27-34
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    • 2015
  • 나노입자의 응용은 1970년대부터 발전되어 왔다. 금속 나노입자에 빛을 조사하면 나노입자 표면에서 플라즈몬 공명(SPR, surface plasmon resonance)을 일으킨다. SPR 효과는 금속표면에 입사한 빛에너지에 의해 전자가 여기하며 공명을 일으켜 진동을 발생시키는 현상을 말한다. 여기 된 전자들이 금속원자들과 충돌을 일으키며 열에너지로 전환될 수 있는데 이를 금속의 광열효과(photothermal effect)라고 한다. 우리는 광열 효과를 이용하여 온열 화장품의 개발 가능성을 제시하고자 한다. 온열 화장품의 개발을 위해 생체 독성이 적은 금 나노입자를 선택하여 광열 효과에 있어서의 세포 생체적합성과 열효율을 살펴보았다. 금 나노입자의 합성 상태는 금 나노입자가 갖는 독특한 흡광 스펙트럼으로 확인하였으며, 금 나노입자의 농도는 원자 흡광분석기로 측정하였다. 세포의 독성평가는 MTT assay와 이중 염색법을 사용하였으며, 금 나노입자의 광열 효과는 제논 램프를 광원으로 하여 금 나노입자의 농도의 증가에 따른 광열 효과증대를 적외선-열화상 카메라로 확인하였다. 금 나노입자의 광열 효과를 온열 화장품에 적용한다면 한대 지방의 기후, 또는 겨울철에 태양 에너지를 열에너지로 전환시켜 피부에 손실된 열을 공급, 피부온도 유지에 도움이 되고 피부건강에 긍정적 효과를 주리라 사료된다.

Facile Preparation of Pyrene-templated Hexagonal-shaped Gold Nanoplates

  • Lim, Eun-Kyung;Jang, Eunji;Haam, Seungjoo;Huh, Yong-Min
    • Applied Science and Convergence Technology
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    • 제23권1호
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    • pp.48-53
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    • 2014
  • We have formulated hexagonal-shaped gold nanoplates in a single-step for photothermal therapy that gold ions to gold particles using pyrenyl dextran as reducible stabilizer and template. They exhibit anisotropic structure with broad surface plasmon resonance (SPR) band into near-infrared (NIR) spectrum enabling photothermal therapy. These gold nanoplates are also confirmed biocompatibility and high uptake efficiency due to binding with dextran molecules on the surface of gold nanoplates and cells. From in vitro phtothermal ablation study under NIR laser, gold nanoplates have the potential to use as photothermal agents.

Minor Coat Protein pIII Domain (N1N2) of Bacteriophage CTXф Confers a Novel Surface Plasmon Resonance Biosensor for Rapid Detection of Vibrio cholerae

  • Shin, Hae Ja;Hyeon, Seok Hywan;Cho, Jae Ho;Lim, Woon Ki
    • 한국미생물·생명공학회지
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    • 제49권4호
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    • pp.510-518
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    • 2021
  • Bacteriophages are considered excellent sensing elements for platforms detecting bacteria. However, their lytic cycle has restricted their efficacy. Here, we used the minor coat protein pIII domain (N1N2) of phage CTXφ to construct a novel surface plasmon resonance (SPR) biosensor that could detect Vibrio cholerae. N1N2 harboring the domains required for phage adsorption and entry was obtained from Escherichia coli using recombinant protein expression and purification. SDS-PAGE revealed an approximate size of 30 kDa for N1N2. Dot blot and transmission electron microscopy analyses revealed that the protein bound to the host V. cholerae but not to non-host E. coli K-12 cells. Next, we used amine-coupling to develop a novel recombinant N1N2 (rN1N2)-functionalized SPR biosensor by immobilizing rN1N2 proteins on gold substrates and using SPR to monitor the binding kinetics of the proteins with target bacteria. We observed rapid detection of V. cholerae in the range of approximately 103 to 109 CFU/ml but not of E. coli at any tested concentration, thereby confirming that the biosensor exhibited differential recognition and binding. The results indicate that the novel biosensor can rapidly monitor a target pathogenic microorganism in the environment and is very useful for monitoring food safety and facilitating early disease prevention.

Development of SPR Gas Sensor for Small Molecules Using Molecularly Imprinted Polymer Thin Films

  • 장성우;진성일;박찬량
    • 한국재료학회:학술대회논문집
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    • 한국재료학회 2011년도 춘계학술발표대회
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    • pp.242.2-242.2
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    • 2011
  • Molecularly imprinted polymer thin films were applied to develop a gas sensor based on the surface plasmon resonance phenomenon for small gaseous molecules such as toluene and xylene. The imprinted polymer films were synthesized via photo-polymerization method using various combination of templates, functional monomers and cross-linkers. The temperature of pre-polymerization solutions and the power of UV light were controlled for optimized performance of gas sensing. The morphology and porosity of the polymer films were controlled by varying the mixing ratios of the pre-polymerization solutions and confirmed by atomic force microscopy. By fitting the adsorption/desorption sensorgrams to conventional kinetic models, the effects of different templates and cross-linkers were interpreted in term of the structural differences of the polymer networks formed on the gold film. The sensitivity and selectivity of sensors were estimated for toluene and xylene, and also for humidity and other gaseous molecules such as formaldehyde and ammonia.

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광섬유 표면 플라즈몬 공명 센서를 이용한 스트레인 및 온도 측정 (Measurement of temperature and strain using fiber-optic surface plasmon resonance sensor)

  • 정재훈;김민욱
    • 전기전자학회논문지
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    • 제11권2호
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    • pp.77-82
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    • 2007
  • 본 논문에서는 광섬유 표면 플라즈몬 공명 센서를 이용하여 스트레인 및 온도 변화에 따른 센서를 제시하였다. 광섬유 센서의 구조는 광섬유의 클래딩 일부를 제거하여 크롬, 은, 금을 증착한 다층 박막 구조를 모델로 하였고, dip 파장의 변화가 선형성을 보임으로써 효율적인 센서로의 활용 가능성을 보이고자 한다.

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SPR 바이오센서를 이용한 미생물 인식 시스템 (A System to Recognize Microorganisms Using SPR Biosensor)

  • 조용진;김남수
    • 한국농업기계학회:학술대회논문집
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    • 한국농업기계학회 2003년도 동계 학술대회 논문집
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    • pp.200-208
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    • 2003
  • 미생물수를 측정하는 가장 정확하고 보편적인 방법은 배양법이다. 그러나 배양법은 많은 시간과 노력이 소요되기 때문에 현장에서 실시간에 준하는 측정법으로 사용하기에는 원천적으로 한계를 가지고 있다. 이러한 한계를 극복하기 위해서는 실시간(real-time) 또는 준실시간(near real-time)으로 미생물을 검출할 수 있는 기술이 요구된다. 표면플라즈몬공명(surface plasmon resonance: SPR)은 생물분자 또는 미생물을 실시간 또는 준실시간으로 검출할 수 있는 센서로서 특이성(specificity)과 정확도(accuracy) 측면에서 일찍이 큰 관심을 받아왔다. 1902년, Wood(1902)는 반사 회절격자를 사용하여 연속광원의 스펙트럼을 관찰한 결과, 회절광 스펙트럼에서 어두운 좁은 밴드를 발견하였으며, Fano(1941)는 이 현상이 surface plasma waves와 관련이 있음을 이론적으로 밝혔다. (중략)

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