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http://dx.doi.org/10.5369/JSST.2019.28.6.355

Immunoaffinity Characteristics of Exosomes from Breast Cancer Cells Using Surface Plasmon Resonance Spectroscopy  

Sohn, Young-Soo (Department of Biomedical Engineering, Daegu Catholic University)
Na, Wonhwi (Nano Biofluignostics Research Center, Korea University)
Jang, Dae-Ho (Nano Biofluignostics Research Center, Korea University)
Publication Information
Journal of Sensor Science and Technology / v.28, no.6, 2019 , pp. 355-359 More about this Journal
Abstract
Exosomes, known as nanoscale extracellular vesicles in the range of 30-150 nm, are known to contain clinically significant information. However, there is still insufficient information on exosomal membrane proteins for cancer diagnosis. In this work, we investigated the characteristics of the membrane proteins of exosomes shed by cultured breast cancer cell lines using a surface plasmon resonance (SPR) spectroscopy and pre-activated alkanethiols modified sensor chips. The antibodies of breast cancer biomarkers such as MCU-16, EpCAM, CD24, ErbB2, and CA19-9 were immobilized on the pre-activated alkanethiols surfaces without any activation steps. The purified exosomes were loaded onto each antibody surface. The affinity rank of the antibody surfaces was decided by the relative capture efficiency factors for the exosomes. In addition, an antibody with a relative capture efficiency close to 100% was tested with exosome concentration levels of 104/µl, 105/µl, and 106/µl for quantitative analysis.
Keywords
Exosome; Breast cancer; Surface plasmon resonance; Relative capture efficiency factor; Antibody;
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1 W. Wang, X. Xu, B. Tian, Y. Wang, L. Du, T. Sun, Y. Shi, X. Zhao, and J. Jing, "The diagnostic value of serum tumor markers CEA. CA19-9, CA125, CA15-3, and TPS in metastatic breast cancer", Clin. Chim. Acta, Vol. 470, pp. 51-55, 2017.   DOI
2 H. Sipova and J. Homola, "Surface plasmon resonance sensing of nucleic acids: A review", Anal. Chim. Acta, Vol. 773, pp. 9-23, 2013.   DOI
3 C. Liu, F. Hu, W. Yang, J. Xu, and Y. A. Chen, "Critical review of advances in surface plasmon resonance imaging sensitivity", Trends Anal. Chem., Vol. 97, pp. 354-362, 2017.   DOI
4 C. Lertvachirapaiboon, A. Baba, S. Ekgasit, K. Shinbo, K. Kato, and F. Kaneko, "Transmission surface plasmon resonance techniques and their potential biosensor applications", Biosens. Bioelectron., Vol. 99, pp. 399-415, 2018.   DOI
5 C. G. Zhang, S. J. Chang, K. Settu, C. J. Chen, and J. T. Liu, "High-sensitivity glycated hemoglobin (HbA1c) aptasensor in rapid-prototyping surface plasmon resonance", Sens. Actuators B, Vol. 279, pp. 267-273, 2019.   DOI
6 S. G. Patching, "Surface plasmon resonance spectroscopy for characterisation of membrane protein-ligand interactions and its potential for drug discovery," Biochim. Biophys. Acta Biomembr., Vol. 1838, pp. 43-55, 2014.   DOI
7 M. Mahmoudpour, J. E. N. Dolatabadi, M. Torbati, and A. Homayouni-Rad, "Nanomaterials based surface plasmon resonance signal enhancement for detection of environmental pollutions", Biosens. Bioelectron., Vol. 127, pp. 72-84, 2019.   DOI
8 B. J. Yakes, J. Buijs, C. T. Elliott, and K. Campbell, "Surface plasmon resonance biosensing: Approaches for screening and characterizing antibodies for food diagnostics", Talanta, Vol. 156-157, pp. 55-63, 2016.   DOI
9 C. Lawson, J. M. Vicencio, D. M. Yellon, and S. M. Davidson, "Microvesicles and exosomes: new players in metabolic and cardiovascular disease", J. Endocrinol., Vol. 288, pp. R57-R71, 2016.
10 S. H. Jalalian, M. Ramezani, S. A. Jalalian, K. Abnous, and S. M. Taghdisi, "Exosomes, new biomarkers in early cancer detection", Anal. Biochem., Vol. 571, pp. 1-13, 2019.   DOI
11 L. Console, M. Scalise, and C. Indiveri, "Exosomes in inflammation and role as biomarkers", Clin. Chim. Acta, Vol. 488, pp. 165-171, 2019.   DOI
12 M. R. Fernando, C. Jiang, G. D. Krzyzanowski, and W. L. Ryan, "New evidence that a large proportion of human blood plasma cell-free DNA is localized in exosomes," PLoS ONE, Vol. 12, pp. e0183915(1)-e0183915(15), 2017.   DOI
13 T. Pisitkun, R. F. Shen, and M. A. Knepper, "Identification and proteomic profiling of exosomes in human urine", Proc. Natl. Acad. Sci. U.S.A., Vol. 101, pp. 13368-13373, 2004.   DOI
14 V. Palanisamy, S. Sharma, A. Deshpande, H. Zhou, J. Gimzewski, and D. T. Wong, "Nanostructural and transcriptomic analyses of human saliva derived exosomes", PLoS ONE, Vol. 5, pp. e8577(1)-e8577(11), 2010.   DOI
15 D. L. M. Rupert, C. Lasser, M. Eldh, S. Block, V. P. Zhdanov, J. O. Lotvall, M. Bally, and F. Hook, "Determination of Exosome Concentration in Solution Using Surface Plasmon Resonance Spectroscopy", Anal. Chem., Vol. 86, pp. 5929-5936, 2014.   DOI
16 C. Admyre, S. M. Johansson, K. R. Qazi, J.-J. Filen, R. Lahesmaa, M. Norman, E. P. A. Neve, A. Scheynius, and S. Gabrielsson, "Exosomes with Immune Modulatory Features Are Present in Human Breast Milk", J. Immunol., Vol. 179, pp. 1969-1978, 2007.   DOI
17 S. W. Ferguson and J, Nguyen, "Exosomes as therapeutics: The implications of molecular composition and exosomal heterogeneity", J. Control. Release, Vol. 228, pp. 179-190, 2016.   DOI
18 O. P. Kallioniemi, A. Kallioniemi, W. Kurisu, A. Thor, L. C. Chen, H. S. Smith, F. M. Waldman, D. Pinkel, and J. W. Gray, "ERBB2 amplification in breast cancer analyzed by fluorescence in situ hybridization", Proc. Natl. Acad. Sci. U.S.A., Vol. 89, pp. 5321-5325, 1992.   DOI
19 I. Helwa, J. Cai, M. D. Drewry, A. Zimmerman, M. B. Dinkins, M. L. Khaled, M. Seremwe, W. Michael Dismuke, E. Bieberich, W. D. Stamer, M. W. Hamrick, and Y. Liu, "Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents", PLoS ONE, Vol. 12, pp. e0170628(1)-e0170628(22), 2017.   DOI
20 C. Beyer and D. S. Pisetsky, "The role of microparticles in the pathogenesis of rheumatic diseases", Nat. Rev. Rheumatol., Vol. 6, pp. 21-29, 2010.   DOI
21 L. A. Torre, F. Bray, R. L. Siegel, J. Ferlay, J. Lortet-Tieulent, and A. Jemal, "Global cancer statistics, 2012", CA. Cancer J. Clin., Vol. 65, pp. 87-108, 2015.   DOI
22 R. Siegel, D. Naishadham, and A. Jemal, "Cancer statistics, 2012", CA. Cancer J. Clin., Vol. 62, pp. 10-29, 2012.   DOI
23 S. Reinartz, S. Failer, T. Schuell, and U. Wagner, "CA125 (MUC16) gene silencing suppresses growth properties of ovarian and breast cancer cells", Eur. J. Cancer, Vol. 48, pp. 1558-1569, 2012.   DOI
24 N. M. R. Abd El-Maqsoud and D. M. Abd El-Rehim, "Clinicopathologic Implications of EpCAM and Sox2 Expression in Breast Cancer", Clin. Breast Cancer, Vol. 14, pp. e1-e9, 2014.   DOI
25 U. Eletxigerra, J. Martinez-Perdiguero, S. Merino, R. Barderas, R. M. Torrente-Rodriguez, R. Villalonga, J. M. Pingarron, and S. Campuzano, "Amperometric magnetoimmunosensor for ErbB2 breast cancer biomarker determination in human serum, cell lysates and intact breast cancer cells", Biosens. Bioelectron., Vol. 70, pp. 34-41, 2015.   DOI
26 K. Suyama, H. Onishi, A. Imaizumi, K. Shinkai, M. Umebayashi, M. Kubo, Y. Mizuuchi, Y. Oda, M. Tanaka, M. Nakamura, and M. Katano, "CD24 suppresses malignant phenotype by downregulation of SHH transcription through STAT1 inhibition in breast cancer cells", Cancer Lett., Vol. 374, pp. 44-53, 2016.   DOI