• Korean Journal of Agricultural Science
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• v.50 no.4
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• pp.917-925
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• 2023

Efforts for developing the domestic horse industry in South Korea are evident through the various national policies. Proper management of stables for ensuring the health of horses is essential to sustain the growing equine industry. This study aimed to measure the fine dust emissions in stables based on the type and quantity of bedding used for horses, for establishing guidelines pertaining to bedding use in stables. The stables accommodated 12 horses. Sawdust, wood shavings, wood pellet, and straw were chosen as treatments. Three different quantities (approximately 3, 6, and 9 cm in height) were applied for each type. Fine dust measurements were carried out at three time points, with each measuring period lasting for approximately three weeks. Measurements included PM2.5 and PM10 fine dust levels. The initial analysis revealed that, sawdust with 9 cm bedding had the highest dust levels; approximately 54.6 ㎍/m³, for PM2.5 and 95.3 ㎍/m³, for PM10. Sawdust bedding at the highest quantity (9 cm) exhibited significantly higher initial fine dust emissions. These findings suggest that bedding materials with smaller particle size, such as sawdust and wood shavings, tend to produce finer dust. Initially, the fine dust emissions decreased in all bedding types and quantities, possibly due to the increased moisture content of bedding owing to horse manure production. However, emissions increased subsequently due to ammonia production.

• The Korean Journal of Malacology
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• v.30 no.1
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• pp.57-77
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• 2014

The bivalve molluscan fauna of Ulsan Bay, East Sea coast of Korea, is summarized, based on original and literature data. The fauna consists of 61 species belonging to 20 families. Seven species are identified only to genus level. Two species (Carditellopsis toneana (Yokoyama, 1922), Carditidae and Fulvia hungerfordi (G.B. Sowerby III, 1901), Cardiidae) are new records for the East Sea coast of Korea, and one species (Crenella decussata (Montagu, 1808), Mytilidae) is a new record for Korea. Biogeographically, Ulsan Bay's bivalve fauna is subtropical with a predominance of tropical-subtropical species, 21 species, or 39% of the total species number, subtropical, 14 species, or 26%, and subtropical-boreal (mostly subtropical-lowboreal), 11 species, 21%, totalling 86%. A remarkable feature of the Ulsan Bay fauna is the presence of tropical-subtropical species not found in Yeongil Bay but common in tidal flats and shallow waters of the Yellow Sea and the southern part of Korea. A cold water mass appearing off the southeast coast of Korea near Ulsan in summer seems responsible for the presence of boreal-arctic species in this area.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.10
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• pp.1407-1415
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• 2016

Isolation and culture of spermatogonial stem cells (SSCs) are attractive for production of genetic modified offspring. In the present study, buffalo spermatogonial stem-like cells were isolated, cultured and expression pattern of different germ cell marker genes were determined. To recover spermatogonia, testes from age 3 to 7 months of buffalo were decapsulated, and seminiferous tubules were enzymatically dissociated. Two types of cells, immature sertoli cell and type A spermatogonia were observed in buffalo testes in this stage. Germ cell marker genes, OCT3/4 (Pou5f1), THY-1, c-kit, PGP9.5 (UCHL-1) and Dolichos biflorus agglutinin, were determined to be expressed both in mRNA and protein level by reverse transcription polymerase chain reaction and immunostaining in buffalo testes and buffalo spermatogonial stem-like cells, respectively. In the following, when the isolated buffalo buffalo spermatogonial stem-like cells were cultured in the medium supplemented 2.5% fetal bovine serum and 40 ng/mL glial cell-derived neurotrophic factor medium, SSCs proliferation efficiency and colony number were significantly improved than those of other groups (p<0.05). These findings may help in isolation and establishing long term in vitro culture system for buffalo spermatogonial stem-like cells, and accelerating the generation of genetic modified buffaloes.

• Journal of Embryo Transfer
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• v.28 no.3
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• pp.223-227
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• 2013

The objective of the study is to assess temperament of a horse based on general temperament test by a questionnaire survey. Five test criteria were identified: gentleness, patience, aggressiveness, sensitivity, and friendliness, each on a 5-point scale. 114 horses bred at the National Institute of Animal Science (NIAS) in 2010, 2011 and 2012. The horses recorded scores of 3.6~3.9 for gentleness, 3.1~3.6 for patience, 3.4~4.0 for aggressiveness, 2.8~3.2 for sensitivity, and 3.4~3.8 for friendliness, the overall score for sensitivity the lowest. Horses born in 2012 scored lower than the rest in all five areas at a statistically significant level (P<0.05). By gender, the colts scored higher than the fillies in all five areas, but the discrepancy was not statistically significant. Factor analysis yielded only one factor, and the Cronbach's ${\alpha}$ value was 0.980 for standardization of Factor 1, indicating a high reliability of internal consistency. The correlation coefficients among the test criteria ranged between 0.85 and 0.91 (P<0.01). The assessment criteria used in this study are expected to provide a useful basis designing a temperament test horses.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1098-1102
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• 2018

Objective: Temperament can be defined as a type of behavioral tendency that appears in a relatively stable manner in responses to various external stimuli over time. The aim of this study was to estimate genetic parameters for the records of temperament testing that are used to improve the temperament of Jeju crossbred (Jeju${\times}$Thoroughbred) horses. Methods: This study was conducted using 205 horses (101 females and 104 males) produced between 2010 and 2015. The experimental animals were imprinted and tamed according to the Manual for Horse Taming and Evaluation for Therapeutic Riding Horses and evaluated according to the categories for temperament testing (gentleness, patience, aggressiveness, sensitivity, and friendliness) between 15 months and 18 months of age. Each category was scored on a five-point linear scale. Genetic parameters for the test categories were analyzed using a multi-trait mixed model with repeated records. The ASReml program was used to analyze the data. Results: The heritability of gentleness, patience, aggressiveness, sensitivity and friendliness ranged from 0.08 to 0.53. The standard errors of estimated heritability ranged from 0.13 to 0.17. The test categories showed high genetic correlations with each other, ranging from 0.96 to 0.99 and high repeatability, ranging from 0.70 to 0.73. Conclusion: The results of this study showed that the test categories had moderate heritability and high genetic correlations, but additional studies may be necessary to use the results for the improvement programs of the temperament of Jeju crossbred horses.

• Journal of Animal Science and Technology
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• v.65 no.3
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• pp.511-518
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• 2023

This study examined the association between functional sequence variants (FSVs) of myosin heavy chain 3 (MYH3) genotypes and collagen content in a Landrace and Jeju native pig (JNP) crossbred population. Four muscles (Musculus longissimus dorsi, Musculus semimembranosus, Musculus triceps brachii, and Musculus biceps femoris) were used for the analysis of meat collagen content, and the same animals were genotyped for the FSVs of the MYH3 gene by using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Three FSVs of MYH3 genotypes were identified and had genotype frequencies of 0.358, 0.551, and 0.091 for QQ, Qq, and qq, respectively. QQ animals for the FSVs of the MYH3 genotypes showed higher collagen content in their M. longissimus dorsi (p < 0.001), M. semimembranosus (p < 0.001), M. triceps brachii (p < 0.001), and M. biceps femoris (p < 0.001) than qq homozygous animals. After the validation of this result in other independent populations, the FSVs of MYH3 genotypes can be a valuable genetic marker for improving collagen content in porcine muscles and can also be applied to increase the amount of collagen for biomedical purposes.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.57 no.4
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• pp.467-478
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• 2024

The fish assemblage characteristics in the coastal waters of Sinchang-ri in the Western region of Jeju Island were determined using trammel net samples collected from 2012 to 2022. During the survey period, 81 fish species (43 families, 12 orders) were identified. Tetraodontiformes (4 families, 9 species) and Perciformes (26 families, 57 species) accounted for 84% of the total number. Annual species variation was highest in 2017 and 2019 (36 species) and lowest in 2015 (26 species). Seasonal species variation was higher in summer (peak, 58 species) than winter (peak, 41 species). The number of individuals (1,509) and biomass (446.5 kg) peaked in August (summer) and were lowest in February (winter; 415 individuals, and 150.7 kg). 41 (50.6%) subtropical fish species were identified, and the number of species was highest in 2020 {21 (61.8%) and lowest in 2014, 2017 [16-18 (47.1-47.4%)]}. Subtropical fish species, individuals and biomass were significantly correlated with environmental factors (summer temperatures) at the sampling sites. Scarbreast tuskfish Choerodon azurio, balloon fish Diodon holocanthus, flag fish Goniistius zonatus, bluestriped angelfish Chaetodontoplus septentrionalis, hairy stingfish Scorpaenopsis cirrhosa, threeline grunt Parapristipoma trilineatum, and blackfin sweeper Pempheris japonica were the dominant species of subtropical fish in the study sites.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.4
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• pp.500-508
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• 2016

This study was conducted to investigate the effects of Momordica charantia saponin (MCS) on ruminal fermentation of maize stover and abundance of selected microbial populations in vitro. Five levels of MCS supplements (0, 0.01, 0.06, 0.30, 0.60 mg/mL) were tested. The pH, $NH_3-N$, and volatile fatty acid were measured at 6, 24, 48 h of in vitro mixed incubation fluids, whilst the selected microbial populations were determined at 6 and 24 h. The high dose of MCS increased the initial fractional rate of degradation at t-value = 0 ($FRD_0$) and the fractional rate of gas production (k), but decreased the theoretical maximum of gas production ($V_F$) and the half-life ($t_{0.5}$) compared with the control. The $NH_3-N$ concentration reached the lowest concentration with 0.01 mg MCS/mL at 6 h. The MSC inclusion increased (p<0.001) the molar proportion of butyrate, isovalerate at 24 h and 48 h, and the molar proportion of acetate at 24 h, but then decreased (p<0.05) them at 48 h. The molar proportion of valerate was increased (p<0.05) at 24 h. The acetate to propionate ratio (A/P; linear, p<0.01) was increased at 24 h, but reached the least value at the level of 0.30 mg/mL MCS. The MCS inclusion decreased (p<0.05) the molar proportion of propionate at 24 h and then increased it at 48 h. The concentration of total volatile fatty acid was decreased (p<0.001) at 24 h, but reached the greatest concentration at the level of 0.01 mg/mL and the least concentration at the level of 0.60 mg/mL. The relative abundance of Ruminococcus albus was increased at 6 h and 24 h, and the relative abundance of Fibrobacter succinogenes was the lowest (p<0.05) at 0.60 mg/mL at 6 h and 24 h. The relative abundance of Butyrivibrio fibrisolvens and fungus reached the greatest value (p<0.05) at low doses of MCS inclusion and the least value (p<0.05) at 0.60 mg/mL at 24 h. The present results demonstrates that a high level of MCS quickly inhibits in vitro fermentation of maize stover, while MCS at low doses has the ability to modulate the ruminal fermentation pattern by regulating the number of functional rumen microbes including cellulolytic bacteria and fungi populations, and may have potential as a feed additive applied in the diets of ruminants.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.7
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• pp.1092-1099
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• 2007

Two experiments were conducted to determine the additivity of apparent or true digestibility of phosphorus (P) in soybean meal (SBM), peas, faba beans, corn, oats, broken rice meal, rough rice meal, buckwheat, and sorghum for growing pigs. Chromic oxide (0.3%) was used as a digestion marker in both experiments. Each experiment lasted for 12 d, which consisted of a 7-d dietary adaptation period followed by a 5-d fecal collection period. Experiment 1 involved 6 diets: the SBM-based control diet; 4 diets with corn, oats, rough rice meal and broken rice meal substituted for SBM; and an additional diet with a representative mixture of the 5 ingredients. In Experiment 2, 6 diets were prepared similarly, except that the tested ingredients besides SBM were faba beans, peas, buckwheat, and sorghum. In each experiment, six barrows with an initial average individual BW of 20.5 kg were fed one of the six diets according to a $6{\times}6$ Latin square design. The apparent and true P digestibility values for the nine tested ingredients were determined by the substitution method. There were no differences (p>0.05) between the determined and the predicted true P digestibility values for the mixture of ingredients in Experiments 1 and 2. However, the determined and the predicted apparent P digestibility values for the mixture of ingredients differed (p = 0.059) in Experiment 1, but not in Experiment 2. These results indicate that true P digestibility values are additive in ingredients containing low levels of phytate phosphorus and anti-nutritional factors, whereas the apparent P digestibility values are not always additive in single feed ingredients for growing pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.2
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• pp.230-240
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• 2016

Information on the effects of different yeast species on ruminal fermentation is limited. This experiment was conducted in a $3{\times}4$ factorial arrangement to explore and compare the effects of addition of three different live yeast species (Candida utilis 1314, Saccharomyces cerevisiae 1355, and Candida tropicalis 1254) at four doses (0, $0.25{\times}10^7$, $0.50{\times}10^7$, and $0.75{\times}10^7$ colony-forming unit [cfu]) on in vitro gas production kinetics, fiber degradation, methane production and ruminal fermentation characteristics of maize stover, and rice straw by mixed rumen microorganisms in dairy cows. The maximum gas production (Vf), dry matter disappearance (IVDMD), neutral detergent fiber disappearance (IVNDFD), and methane production in C. utilis group were less (p<0.01) than other two live yeast supplemented groups. The inclusion of S. cerevisiae reduced (p<0.01) the concentrations of ammonia nitrogen ($NH_3$-N), isobutyrate, and isovalerate compared to the other two yeast groups. C. tropicalis addition generally enhanced (p<0.05) IVDMD and IVNDFD. The $NH_3$-N concentration and $CH_4$ production were increased (p<0.05) by the addition of S. cerevisiae and C. tropicalis compared with the control. Supplementation of three yeast species decreased (p<0.05) or numerically decreased the ratio of acetate to propionate. The current results indicate that C. tropicalis is more preferred as yeast culture supplements, and its optimal dose should be $0.25{\times}10^7$ cfu/500 mg substrates in vitro.