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Effects of fermented soybean meal with Bacillus velezensis, Lactobacillus spp. or their combination on broiler performance, gut antioxidant activity and microflora

  • Tsai, C.F.;Lin, L.J.;Wang, C.H.;Tsai, C.S.;Chang, S.C.;Lee, T.T.
    • Animal Bioscience
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    • v.35 no.12
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    • pp.1892-1903
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    • 2022
  • Objective: A series of experiment were conducted to evaluate the effects of replacing a part of soybean meal (SBM) at 6% of broiler diets with fermented soybean meal (FSBM) obtained by single or two-stage fermentation by measuring growth performance, antioxidant activity in the jejunum and distal intestinal microflora. Methods: Soybean meal samples were prepared by single-stage fermentation using Bacillus velezensis (Bv) (FSBMB), or Lactobacillus spp. (as commercial control) (FSBML). Additional SBM sample was prepared by two-stage fermentation using Bv and subsequently using Lactobacillus brevis ATCC 367 (Lb) (FSBMB+L). Enzyme activity, chemical composition, trichloroethanoic acid-nitrogen solubility index (TCA-NSI) and antioxidant activity were measured. Then, in an in vivo study, 320 Ross308 broilers were divided into four groups with ad libitum supply of feed and water. Four groups were fed either a corn-soybean meal diet (SBM), or one of fermented SBM diets (FSBMB+L, FSBMB, and FSBML). Growth, serum characteristics, microflora, and the mRNA expression of selected genes were measured. Results: Compared to SBM, FSBMB+L contained lower galacto-oligosaccharide, allergic protein, and trypsin inhibitor, and higher TCA-NSI by about three times (p<0.05). Reducing power and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging ability correlated positively with the TCA-NSI content in FSBM. Growth performances were not significantly different among four groups. In jejunum of 35-day-old broilers, partial replacement of SBM by FSBMB+L increased the activity of superoxide dismutase and catalase (CAT), and the FSBMB group had the highest catalase activity (p<0.05). Partial replacement of SBM by FSBM increased relative mRNA expressions of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and peptide transporter 1 (PepT1) (p<0.05); however, FSBMB+L increased CAT mRNA level to 5 times of the control (p<0.05). Conclusion: Using Bv- and Lb-processed SBM through two-stage fermentation to partially replace 6% of diets will improve the gut's antioxidant activity under commercial breeding in broilers.

Development of the Exocarp and Occurrence of Micro-cracking in 'Jinmi' Peaches (복숭아 '진미'의 외과피 발달과 미세균열)

  • Ma, Kyeong-Bok;Chun, Jong-Pil;Kim, Jung-Bae;Do, Kyung-Ran;Cho, Kwang-Sik;Choi, Jin-Ho;Hwang, Hae-Sung
    • Horticultural Science & Technology
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    • v.30 no.1
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    • pp.1-5
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    • 2012
  • This study was conducted to elucidate the cause of micro-cracking in the exocarp of 'Jinmi' peach (Prunus persica). through the microscopic observation of fruit skin development in 4 varieties such as 'Jinmi', 'Kanoiwa Hakuto', 'Kawanakajima Hakuto', and 'Yumyeong'. Micro-cracking was noted in 59.1% of 'Jinmi', 30.6% of 'Kanoiwa Hakuto', 21.5% 'Kawanakajima Hakuto' and 6.4% of 'Yumyeong', respectively. The development of intercellular spaces, which increased rapidly with the fruit development, was easily observed at 69 days after full bloom. Histological studies revealed that the number of outer epiderm cell layers of 'Jinmi' was smaller than that of the other three cultivars, and thinner than the 'Kawanakajima Hakuto' and 'Yumyeong'. Moreover, 'Jinmi' exhibited smaller and flatter shapes in the sub-epidermal cell layer than those of the 'Kawanakajima Hakuto' and 'Yumyeong' at harvest season. Therefore, these results suggest that micro-cracking of 'Jinmi' fruit skin was due to poor-developed outer epidermis and well-developed intercellular spaces just under exocarp as compared with other varieties.

Distribution of Soil and Water Temperatures and their Relation to Rice Growth in a Cold Water Irrigated Paddy Land (냉수(冷水) 관개답(灌漑畓)에서의 수온 및 지온 분포(分布)와 수도(水稻) 생육(生育) 및 수량 반응)

  • Kim, Lee-Yul;Im, Jeong-Nam;Yea, Jong-Doo;Kim, Dong-Jin
    • Korean Journal of Soil Science and Fertilizer
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    • v.15 no.4
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    • pp.221-225
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    • 1982
  • Soil and water temperature were measured at different distances from the irrigation inlet of a Paddy land supplied with $16^{\circ}C$ water. The rice growth and yields of Nongbaek variety (Japonica) and Milyang 23 variety (Indica${\times}$Japonica)were interrelated with average soil and water temperature from tillering to heading stage. Water temperature was asymtotically increased to $21^{\circ}C$ as cold water forwarded, while the distribution of soil temperature showed linear shape with distance overall, water temperature was 1 to $2^{\circ}C$ higher than soil temperature. Cold soil and water temperature shorterned culm length and panicle exertion and retarded heading date resulting in yield reduction, Response of rice growth and yield to water temperature was apparently different between two varieties. Nongbaek variety produced no grains below $17^{\circ}C$, but it's reproductive growth was possible from 17 to $20^{\circ}C$. A normal yield could be obtained at $21^{\circ}C$. Comparatively, no reproductive growth of Milyang 23 variety was Possible even at $19^{\circ}C$.

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The Local Effects of Coal-fired Power Plant Shutdown on PM2.5 Concentration: Evidence from a Policy Experiment in Korea (노후 석탄화력발전소 가동중단에 따른 발전소 주변지역의 초미세먼지 농도 감소효과 분석)

  • Yi, Donggyu;Sung, Jae-hoon
    • Environmental and Resource Economics Review
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    • v.27 no.2
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    • pp.315-337
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    • 2018
  • Korean government temporarily shut down the coal-fired power plants built before 30 years and more from 6/1/2017 to 6/31/2017. This treatment provides a credible natural experiment regarding the regional $PM_{2.5}$ concentration and coal-fired generators. Based on this feature of the treatment, this study analyzed the causality between the old coal-fired power plants and regional $PM_{2.5}$ concentration. To be specific, we categorized two pollution monitoring stations nearby coal-fired power plants in Yeongdong into a treatment station and a control station based on the distance from the power plants. The control station is similar to the treatment station geographically and topographically, but its $PM_{2.5}$ concentration would not be directly affected by coal-fired power plants in Yeongdong. A difference-in-difference method was applied to identify the effects of the old coal-fire power plants on regional $PM_{2.5}$ concentration. The results show that the temporary shutdown would decrease $PM_{2.5}$ concentration nearby coal-fired power plants in Yeongdong by $3.7{\sim}4.4{\mu}g/m^3$.

Mode of Action of Streptomycin Resistance in the Citrus Canker Pathogen (Xanthomonas smithii subsp. citri) in Jeju Island

  • Hyun, Jae-Wook;Kim, Hyo-Jung;Yi, Pyoung-Ho;Hwang, Rok-Yeon;Park, Eun-Woo
    • The Plant Pathology Journal
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    • v.28 no.2
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    • pp.207-211
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    • 2012
  • It has been known that streptomycin resistance in bacteria can occur as a results of chromosomal mutation or through gene acquisition or both. Chromosomal mutations for resistances are point mutations in the rpsL gene, which alter ribosomal protein S12. Acquired resistance has occurred when an $Sm^R$ plasmid carrying transposon Tn5393 with tandem strA-strB gene is transferred by conjugation. A total of 686 isolates of Xanthomonas smithii subsp. citri causal agent of citrus canker disease were collected from 26 citrus orchards in Jeju Island in 2003 and 2004 seasons. Forty-nine of 111 isolates from streptomycin non-sprayed orchards in 2003 season were resistant to streptomycin. Of 107 isolates from orchards sprayed one time with streptomycin, 58 isolates were resistant, and 166 of 221 isolates from orchards sprayed two times with streptomycin were resistant. In 12 orchards sprayed three or more times with streptomycin, 219 of 247 isolates were resistant to streptomycin. Twenty-five isolates of X. smithii subsp. citri were surveyed to identify the mechanisms of streptomycin resistance in this study. Twenty-one of these 25 isolates were resistant to streptomycin, and it was proven by PCR assay that 18 of the 21 streptomycin resistant isolates have the strB gene. In sixteen of the 21 streptomycin resistant isolates, it was occurred a point mutation altered codon lysine (AAG)-41 of rpsL gene to arginine (AGG). The streptomycin-sensitive isolates easily acquired the resistance by mixed culture with resistant isolates. The strB gene was amplified from the isolates that acquired the resistance by mixed culture, and one isolate of them was also point-mutated in codon 41 of rpsL gene to be resistant. In this study, most of the streptomycin-resistant isolates of X. smithii sub sp. citri in Jeju island expressed the resistance by both chromosomal point mutation and gene acquisition, and the resistance was easily acquired through conjugation by culture mixed with streptomycin resistant and sensitive strains.

PRIP, a Novel Ins(1,4,5)P3 Binding Protein, Functional Significance in Ca2+ Signaling and Extension to Neuroscience and Beyond

  • Kanematsu, Takashi;Takeuchi, Hiroshi;Terunuma, Miho;Hirata, Masato
    • Molecules and Cells
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    • v.20 no.3
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    • pp.305-314
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    • 2005
  • Investigation of chemically synthesized inositol 1,4,5-trisphosphate [$Ins(1,4,5)P_3$] analogs has led to the isolation of a novel binding protein with a molecular size of 130 kDa, characterized as a molecule with similar domain organization to phospholipase C-${\delta}1$ (PLC-${\delta}1$) but lacking the enzymatic activity. An isoform of the molecule was subsequently identified, and these molecules have been named PRIP (PLC-related, but catalytically inactive protein), with the two isoforms named PRIP-1 and -2. Regarding its ability to bind $Ins(1,4,5)P_3$ via the pleckstrin homology domain, the involvement of PRIP-1 in $Ins(1,4,5)P_3$-mediated $Ca^{2+}$ signaling was examined using COS-1 cells overexpressing PRIP-1 and cultured neurons prepared from PRIP-1 knock-out mice. Yeast two hybrid screening of a brain cDNA library using a unique N-terminus as bait identified GABARAP ($GABA_A$ receptor associated protein) and PP1 (protein phosphatase 1), which led us to examine the possible involvement of PRIP in $GABA_A$ receptor signaling. For this purpose PRIP knock-out mice were analyzed for $GABA_A$ receptor function in relation to the action of benzodiazepines from the electrophysiological and behavioral aspects. During the course of these experiments we found that PRIP also binds to the b-subunit of $GABA_A$ receptors and PP2A (protein phosphtase 2A). Here, we summarize how PRIP is involved in $Ins(1,4,5)P_3$-mediated $Ca^{2+}$ signaling and $GABA_A$ receptor signaling based on the characteristics of binding molecules.

Change of Physical Properties on Long-Term Fertilization of Compost and Silicate in Paddy Soils (퇴비 및 규산질비료의 장기연용에 따른 토양 물리적특성 변화)

  • Park, Chang-Young;Choi, Jyung;Park, Ki-Do;Jeon, Weon-Tai;Kwon, Hye-Young;Kang, Ui-Gum
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.3
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    • pp.175-181
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    • 2000
  • This study was carried out to investigate the change of soil physical properties in long-term fertilized paddy soils with a Fine silty family of Typic Halpaqueps (Pyeongtaeg series). Treatments fertilized consisted of no fertilizer, compost, NPK, NPK+compost for thirty one years and of NPK+silicate for seventeen years. Water stable aggregate and degree of aggregate stability, which were higher in surface-soil than sub-soil, were high in order of NPK + compost > NPK + silicate > compost > NPK > no fertilizer plot. The ratio of aggregate larger than 0.5mm was high at compost and silicate plots but that smaller than 0.5mm was high at no fertilizer and NPK plots. And this aggregate stability showed negative correlation with soil hardness and bulk density ; positive correlation with sedimentation volume of soils in water. Sedimentation volume of soils in water was a little higher in surface-soil than sub-soil and in wet soil than dry soil, respectively. Pore space ratio and water retention capacity of soils were the most increased by the application of compost and not affected by silicate as in cases of liquid limit and plastic limit. Ignition loss of soils was high in order of NPK + compost > compost > NPK + silicate > NPK > no fertilizer plot. And field shattering ratio of soil mass smaller than 25.4mm was relatively high in NPK + compost, compost, and silicate plots.

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Propagation Environments of a Suburban Area (교외지역 전파환경을 위한 예측모델 제안)

  • Kim, Jae-Sub;Park, Chang-Kyun
    • The Journal of the Acoustical Society of Korea
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    • v.16 no.4
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    • pp.49-56
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    • 1997
  • In mobile communications, it is very important that we predict the propagation environments of radiation pattern, in order to decide the service area, select the best location of the best station, design the cell etc. Therefore, by analyzing the propagation prediction model that is varied according to the kind of antenna, the beam angle, the terrain and obstacles, we expect that the economic operating of communication networks, the calling quality and the service of subscriber will be enhanced. In this paper, we select the around of Seji base station in Naju-city Chonnam for modern suburban area and measure the field strength to propose the optimal propagation prediction model for suburban areas. We propose the propagation prediction model that, it is not found in the other models until now, consists of the correction coefficient with the relative differences of antenna effective height of the base station and mobile station for minimizing errors. Finally, comparing the results of the field test with the computer simulation(PPGIS : Propagation Prediction Geographic Information System) results for the Hata model, the Egri model, the Carey model and the propose model, we confirm the property of the proposed model.

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A Compatibility Study Between New Allocation to Maritime Mobile Satellite Service and Earth Exploration Satellite Service in X-band (X 대역 해상이동위성업무 추가 주파수 분배를 위한 지구탐사위성업무와의 양립성 연구)

  • Oh, Dae-Sub;Jung, Nam-Ho;Kim, Sooyoung
    • Journal of Satellite, Information and Communications
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    • v.8 no.3
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    • pp.41-46
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    • 2013
  • In this paper, we present the compatibility study results including the frequency sharing criteria between new allocation to maritime mobile satellite service and Earth exploration satellite service in the 7/8 GHz. The transmitting Earths station of MMSS in the 8025 - 8400 MHz band would make harmful interference to the receiving Earth station of EESS operating in the same frequency band. In order to ensure the compatibility with EESS, the separation distance is provided as a frequency sharing criteria. The republic of Korea has a plan to launch the geostationary satellite around 2017 and EESS Earth station will be operated in 8025 - 8400 MHz band. Therefore, we calculate the interference levels and separation distance using the system parameters of two Earth station systems. As results of the study, the separation distances for LOS path and Non-LOS path due to the geographical characteristics are shown around 471 km and 200 km, respectively.

The Interface Test between LEO Satellite and Ground Station (저궤도위성과 지상국 간 접속 검증 시험)

  • Kwon, Dong-Young;Jung, Ok-Chul;Kim, HeeSub
    • Aerospace Engineering and Technology
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    • v.11 no.2
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    • pp.49-56
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    • 2012
  • LEO Satellite performs the operations and missions by FSW(Flight Software) after separation from a launch vehicle. Many of the operations by FSW are automatically conducted by the algorithms of FSW. In the case of the IAC(Initial Activation and Checkout) operations, a mission scheduling, an orbit transition, etc, however, a decision and a control of the satellite operators or manufacturers are required in order to operate the satellite safely. For this, the wireless communication channel between a satellite and a ground station should be prepared to receive telemetries and to transmit tele-commands for controlling FSW properly. Therefore, the verification of the interface between KOMPSAT-3 and a ground station is essential. This verification test is named the satellite end-to-end test. In this paper, we show the design process of the satellite end-to-end test and test results.