[KSCI] Korea Science Citation Index Service

PRIP, a Novel Ins(1,4,5)P₃ Binding Protein, Functional Significance in Ca²⁺ Signaling and Extension to Neuroscience and Beyond

Kanematsu, Takashi (Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science and Station for Collaborative Research, Kyushu University)
Takeuchi, Hiroshi (Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science and Station for Collaborative Research, Kyushu University)
Terunuma, Miho (Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science and Station for Collaborative Research, Kyushu University)
Hirata, Masato (Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science and Station for Collaborative Research, Kyushu University)

Abstract

Investigation of chemically synthesized inositol 1,4,5-trisphosphate [ $Ins(1,4,5)P_3$ ] analogs has led to the isolation of a novel binding protein with a molecular size of 130 kDa, characterized as a molecule with similar domain organization to phospholipase C- ${\delta}1$ (PLC- ${\delta}1$ ) but lacking the enzymatic activity. An isoform of the molecule was subsequently identified, and these molecules have been named PRIP (PLC-related, but catalytically inactive protein), with the two isoforms named PRIP-1 and -2. Regarding its ability to bind $Ins(1,4,5)P_3$ via the pleckstrin homology domain, the involvement of PRIP-1 in $Ins(1,4,5)P_3$ -mediated $Ca^{2+}$ signaling was examined using COS-1 cells overexpressing PRIP-1 and cultured neurons prepared from PRIP-1 knock-out mice. Yeast two hybrid screening of a brain cDNA library using a unique N-terminus as bait identified GABARAP ( $GABA_A$ receptor associated protein) and PP1 (protein phosphatase 1), which led us to examine the possible involvement of PRIP in $GABA_A$ receptor signaling. For this purpose PRIP knock-out mice were analyzed for $GABA_A$ receptor function in relation to the action of benzodiazepines from the electrophysiological and behavioral aspects. During the course of these experiments we found that PRIP also binds to the b-subunit of $GABA_A$ receptors and PP2A (protein phosphtase 2A). Here, we summarize how PRIP is involved in $Ins(1,4,5)P_3$ -mediated $Ca^{2+}$ signaling and $GABA_A$ receptor signaling based on the characteristics of binding molecules.

Keywords

Calcium; $GABA_A$ Receptor; GABARAP; $Ins(1,4,5)P_3$ ; KO Mice; PH Domain; Phosphatase;

Citations & Related Records

Times Cited By Web Of Science : 12 (Related Records In Web of Science)

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KSCI

PRIP, a Novel Ins(1,4,5)P3 Binding Protein, Functional Significance in Ca2+ Signaling and Extension to Neuroscience and Beyond

PRIP, a Novel Ins(1,4,5)P₃ Binding Protein, Functional Significance in Ca²⁺ Signaling and Extension to Neuroscience and Beyond