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http://dx.doi.org/10.5713/ab.21.0529

Effects of fermented soybean meal with Bacillus velezensis, Lactobacillus spp. or their combination on broiler performance, gut antioxidant activity and microflora  

Tsai, C.F. (Department of Animal Science, National Chung Hsing University)
Lin, L.J. (School of Chinese Medicine, College of Chinese Medicine, China Medical University)
Wang, C.H. (Central Union Oil Corporation)
Tsai, C.S. (Central Union Oil Corporation)
Chang, S.C. (Kaohsiung Animal Propagation Station, Livestock Research Institute, Council of Agriculture)
Lee, T.T. (Department of Animal Science, National Chung Hsing University)
Publication Information
Animal Bioscience / v.35, no.12, 2022 , pp. 1892-1903 More about this Journal
Abstract
Objective: A series of experiment were conducted to evaluate the effects of replacing a part of soybean meal (SBM) at 6% of broiler diets with fermented soybean meal (FSBM) obtained by single or two-stage fermentation by measuring growth performance, antioxidant activity in the jejunum and distal intestinal microflora. Methods: Soybean meal samples were prepared by single-stage fermentation using Bacillus velezensis (Bv) (FSBMB), or Lactobacillus spp. (as commercial control) (FSBML). Additional SBM sample was prepared by two-stage fermentation using Bv and subsequently using Lactobacillus brevis ATCC 367 (Lb) (FSBMB+L). Enzyme activity, chemical composition, trichloroethanoic acid-nitrogen solubility index (TCA-NSI) and antioxidant activity were measured. Then, in an in vivo study, 320 Ross308 broilers were divided into four groups with ad libitum supply of feed and water. Four groups were fed either a corn-soybean meal diet (SBM), or one of fermented SBM diets (FSBMB+L, FSBMB, and FSBML). Growth, serum characteristics, microflora, and the mRNA expression of selected genes were measured. Results: Compared to SBM, FSBMB+L contained lower galacto-oligosaccharide, allergic protein, and trypsin inhibitor, and higher TCA-NSI by about three times (p<0.05). Reducing power and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging ability correlated positively with the TCA-NSI content in FSBM. Growth performances were not significantly different among four groups. In jejunum of 35-day-old broilers, partial replacement of SBM by FSBMB+L increased the activity of superoxide dismutase and catalase (CAT), and the FSBMB group had the highest catalase activity (p<0.05). Partial replacement of SBM by FSBM increased relative mRNA expressions of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and peptide transporter 1 (PepT1) (p<0.05); however, FSBMB+L increased CAT mRNA level to 5 times of the control (p<0.05). Conclusion: Using Bv- and Lb-processed SBM through two-stage fermentation to partially replace 6% of diets will improve the gut's antioxidant activity under commercial breeding in broilers.
Keywords
Antioxidant; Broiler; Fermented Soybean Meal; Performance; TCA-soluble Peptides;
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1 Hati S, Vij S, Mandal S, Malik RK, Kumari V, Khetra Y. α- Galactosidase activity and oligosaccharides utilization by lactobacilli during fermentation of soy milk. J Food Process Preserv 2014;38:1065-71. https://doi.org/10.1111/jfpp.12063   DOI
2 Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 1959;31:426-8. https://doi.org/10.1021/ac60147a030   DOI
3 Latimer GW. AOAC International. Official methods of analysis of AOAC International. 19th ed. Gaithersburg, MD, USA: AOAC International; 2012.
4 Xie J, Du M, Shen M, Wu T, Lin L. Physico-chemical properties, antioxidant activities and angiotensin-I converting enzyme inhibitory of protein hydrolysates from Mung bean (Vigna radiate). Food Chem 2019;270:243-50. https://doi.org/10.1016/j.foodchem.2018.07.103   DOI
5 Guo S, Zhang Y, Cheng Q, et al. Partial substitution of fermented soybean meal for soybean meal influences the carcass traits and meat. Animals 2020;10:225. https://doi.org/10.3390/ani10020225   DOI
6 Lee MT, Lin WC, Lee TT. Potential crosstalk of oxidative stress and immune response in poultry through phytochemicals-a review. Asian-Australas J Anim Sci 2019;32:309-19. https://doi.org/10.5713/ajas.18.0538   DOI
7 Karas M. Influence of physiological and chemical factors on the absorption of bioactive peptides. Int J Food Sci Technol 2019;54:1486-96. https://doi.org/10.1111/ijfs.14054   DOI
8 Ayyadurai S, Charania MA, Xiao B, Viennois E, Merlin D. PepT1 expressed in immune cells has an important role in promoting the immune response during experimentally induced colitis. Lab Invest 2013;93:888-99. https://doi.org/10.10338/labinvest.2013.77   DOI
9 Handa CL, de Lima FS, Guelfi MFG, Georgetti SR, Ida EI. Multi-response optimisation of the extraction solvent system for phenolics and antioxidant activities from fermented soy flour using a simplex-centroid design. Food Chem 2016;197: 175-84. https://doi.org/10.1016/j.foodchem.2015.10.124   DOI
10 Yin L, Tai HM, Lee HH, Jiang ST. Proteolysis and Lactobacillus fermentation effects on the isoflavones biotransformation and removal of anti-nutritional factors of soy bean. J Mar Sci Technol 2014;22:525-30. https://doi.org/10.6119/JMST014-0613-1   DOI
11 Oyaizu M. Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986;44:307-15. https://doi.org/10.5264/eiyogakuzashi.44.307   DOI
12 Gyamfi MA, Yonamine M, Aniya Y. Free-radical scavenging action of medicinal herbs from Ghana Thonningia sanguinea on experimentally-induced liver injuries. Gen Pharmacol 1999;32:661-7. https://doi.org/10.1016/s0306-3623(98)00238-9   DOI
13 Chachaj R, Sembratowicz I, Krauze M, Ognik K. The effect of partial replacement of soybean meal with fermented soybean meal on chicken performance and immune status. J Anim Feed Sci 2019;28:263-71. https://doi.org/10.22358/jafs/110777/2019   DOI
14 He L, Han M, Qiao S, et al. Soybean antigen proteins and their intestinal sensitization activities. Curr Protein Pept Sci 2015;16:613-21. https://doi.org/10.2174/1389203716666150 630134602   DOI
15 Apajalahti, J, Vienola K. Interaction between chicken intestinal microbiota and protein digestion. Anim Feed Sci Technol 2016;221:323-30. https://doi.org/10.1016/j.anifeedsci.2016.05.004   DOI
16 Soumeh EA, Mohebodini H, Toghyani M, Shabani A, Ashayerizadeh A, Jazi V. Synergistic effects of fermented soybean meal and mannan-oligosaccharide on growth performance, digestive functions, and hepatic gene expression in broiler chickens. Poult Sci 2019;98:6797-807. https://doi.org/10.3382/ps/pez409   DOI
17 Sanjukta S, Rai AK. Production of bioactive peptides during soybean fermentation and their potential health benefits. Trends Food Sci Technol 2016;50:1-10. https://doi.org/10.1016/j.tifs.2016.01.010   DOI
18 Ashaolu TJ. Health applications of soy protein hydrolysates. Int J Pept Res Ther 2020;26:2333-43. https://doi.org/10.1007/s10989-020-10018-6   DOI
19 Cui J, Xia P, Zhang L, Hu Y, Xie Q, Xiang H. A novel fermented soybean, inoculated with selected Bacillus, Lactobacillus and Hansenula strains, showed strong antioxidant and anti-fatigue potential activity. Food Chem 2020;333:127527. https://doi.org/10.1016/j.foodchem.2020.127527   DOI
20 Yang J, Wu X, Chen H, Sun-waterhouse D, Zhong H, Cui C. A value-added approach to improve the nutritional quality of soybean meal by-product: Enhancing its antioxidant activity through fermentation by Bacillus amyloliquefaciens SWJS22. Food Chem 2019;272:396-403. https://doi.org/10.1016/j.foodchem.2018.08.037   DOI
21 Wu P, Golly MK, Guo Y, et al. Effect of partial replacement of soybean meal with high-temperature fermented soybean meal in antibiotic-growth-promoter-free diets on growth performance, organ weights, serum indexes, intestinal flora and histomorphology of broiler chickens. Anim Feed Sci Technol 2020;269:114616. https://doi.org/10.1016/j.anifeedsci.2020.114616   DOI
22 Toe CJ, Foo HL, Loh TC, Mohamad R, Rahim RA, Idrus Z. Extracellular proteolytic activity and amino acid production by lactic acid bacteria isolated from Malaysian foods. Int J Mol Sci 2019;20:1777. https://doi.org/10.3390/ijms20071777   DOI
23 Murugan M, Ragavan A. Broiler performance efficiency factor (BPEF) in commercial broiler production facilities with special reference to climate. Indian Vet J 2017;94:11-4.
24 Yuris A, Siow L. Comparative study of the antioxidant properties of three pineapple (Ananas comosus L.) Varieties. J Food Stud 2014;3:40-56. https://doi.org/10.5296/jfs.v3i1.4995   DOI
25 NRC. Nutrient requirements of poultry. 9th rev. ed. Washington, DC, USA: National Academy Press; 1994.
26 Liu Z, Guan X, Zhong X, Zhou X, Yang F. Bacillus velezensis DP-2 isolated from douchi and its application in soybean meal fermentation. J Sci Food Agric 2020 (2021?);101:1861-8. https://doi.org/10.1002/jsfa.10801   DOI
27 Zhu J, Gao M, Zhang R, et al. Effects of soybean meal fermented by L. plantarum, B. subtilis and S. cerevisieae on growth, immune function and intestinal morphology in weaned piglets. Microb Cell Fact 2017;16:191. https://doi.org/10.1186/s12934-017-0809-3   DOI
28 Ketnawa S, Ogawa Y. Comparative study on protein digestibility, protein patterns, antioxidant activities of raw, cooked and fermented soybeans. J Food Sci Agric Technol 2018;4(Specl Iss.):7-16
29 Mukherjee R, Chakraborty R, Dutta A. Role of fermentation in improving nutritional quality of soybean meal-a review. Asian-Australas J Anim Sci 2016;29:1523-9. https://doi.org/10.5713/ajas.15.0627   DOI
30 Alikwe PCN, Faremi AY, Egwaikhide PA. Biochemical evaluation of serum metabolites, enzymes and haematological indices of broiler-chicks fed with varying levels of rumen epithelial scraps in place of fish meal proteins. Res J Poult Sci 2010;3:27-31. https://doi.org/10.3923/rjpscience.2010.27.31   DOI