• Journal of Plant Biotechnology
• /
• v.43 no.3
• /
• pp.302-310
• /
• 2016

A study aimed at identifying putative drought responsive genes that confer tolerance to water stress deficit in tea plants was conducted in a 'rain-out shelter' using potted plants. Eighteen months old drought tolerant and susceptible tea cultivars were each separately exposed to water stress or control conditions of 18 or 34% soil moisture content, respectively, for three months. After the treatment period, leaves were harvested from each treatment for isolation of RNA and cDNA synthesis. The cDNA libraries were sequenced on Roche 454 high-throughput pyrosequencing platform to produce 232,853 reads. After quality control, the reads were assembled into 460 long transcripts (contigs). The annotated contigs showed similarity with proteins in the Arabidopsis thaliana proteome. Heat shock proteins (HSP70), superoxide dismutase (SOD), catalase (cat), peroxidase (PoX), calmodulinelike protein (Cam7) and galactinol synthase (Gols4) droughtrelated genes were shown to be regulated differently in tea plants exposed to water stress. HSP70 and SOD were highly expressed in the drought tolerant cultivar relative to the susceptible cultivar under drought conditions. The genes and pathways identified suggest efficient regulation leading to active adaptation as a basal defense response against water stress deficit by tea. The knowledge generated can be further utilized to better understand molecular mechanisms underlying stress tolerance in tea.

• Child Health Nursing Research
• /
• v.23 no.1
• /
• pp.70-80
• /
• 2017

Purpose: In this study a caregiver's role improvement program was developed and tested to identify the effect on uncertainty, stress, and role performance of caregivers with hospitalized children. Methods: The design of this study was a quasi-experimental study with a nonequivalent control group and a non-synchronized design. Thirty-three caregivers were assigned to the experimental group and 33 to the control group. Data were collected from March 5 2016 to April 10 2016. For the experimental treatment, each individual was given on-site education with situated learning (given 30 minutes each, for 2 sessions), and self-repetition learning activities were performed from the e-book. Data were analyzed using t-test, ${\chi}^2-test$, Fisher's exact test, paired t-test, and independent t-test. Results: The level of uncertainty and stress decreased, and role performance level improved for these caregivers with hospitalized children. Conclusion: The findings of this study show that using on-site education through situated learning and self-repetition learning with an e-book as in the caregiver's role improvement program is an effective intervention. Therefore, utilizing the caregiver role improvement program developed in this study is recommended as an effective intervention for caregivers of hospitalized children.

• International journal of advanced smart convergence
• /
• v.12 no.4
• /
• pp.443-450
• /
• 2023

One of the basic tasks in the automobile manufacturing process is to design a bare chassis, which is the basic frame of a vehicle, and a bracket is a member connecting various devices to the frame. Bracket, which is a member connecting the engine, transmission, and suspension, which are the core devices of driving and operating the vehicle, to the frame, must maintain safety during vehicle operation. If the bracket connecting the various devices constituting the vehicle to the frame does not have durability, serious accidents may occur during operation of the vehicle. In this study, we performed stress analysis on the brackets installed in the bare chassis of the 25-passenger bus in the development stage. Based on the stress analysis performed, an improved bracket dimension was proposed.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2010.05a
• /
• pp.927-928
• /
• 2010

• The Journal of Korean Obstetrics and Gynecology
• /
• v.26 no.2
• /
• pp.104-119
• /
• 2013

Purpose: The purpose of this study is to recognize menstrual pattern, stress, digestive disorder of female college students who have oligomenorrhea and effects of Gamiguibi-tang. Methods: We researched 30 female students who responded to have menstrual irregulation at ${\bigcirc}{\bigcirc}$ college by questionnaires investigating their menstruation, stress, digestive disorder, and changes of symptoms before and after taking Gamiguibi-tang. Results: 1. There was statistical correlation between menstrual pattern and Qi-Stasis Questionnaire symptoms. 2. There was statistical correlation between menstrual pattern and digestive disorder symptoms. 3. There was statistical correlation between improvement of Qi-Stasis Questionnaire symptoms and usage of Gamiguibi-tang. 4. There was statistical correlation between improvement of digestive disorder symptoms and usage of Gamiguibi-tang. Conclusions: This study showed the stress and digestive disorder symptoms are effectively relieved by Gamiguibi-tang. By this result we expect that Gamiguibi-tang can be used for oligomenorrhea caused by stress or digestive disorder.

• The Journal of Korean Society for School & Community Health Education
• /
• v.15 no.3
• /
• pp.95-103
• /
• 2014

Objectives: To identify work stress and affecting factors of the emergency medical technicians(EMT) and to provide basic data for work circumstance improvement and emergency medical service and duty efficiency elevation. Methods: The data were collected by questionnaire from 635 emergency medical technicians throughout the whole country from August 5 to September 20 2013 and analyzed by using the SPSS(version 18.0) program. Factors affecting stress were analyzed by Multiple Linear Regression Analysis(${\alpha}=0.05$). Results: Mean level of job stress was markedly high in the spot situation(3.43 point), user awareness(3.31 point), burden related to work(3.21 point), role and conflict as a professional job(3.10 point), unsuitable treatment(2.95 point) but was lowest in specialty demand of knowledge and skill(2.76 point). Conclusions: To diminish work stress, understanding and cooperation of the residents and reduction of burden related to work must be improved. Above all, positive interest of 119 and related organ and duty environment improvement will and effort and continuous measure establishment and support and cooperation of the government are needed.

• Corrosion Science and Technology
• /
• v.23 no.4
• /
• pp.266-277
• /
• 2024

Due to its good corrosion and heat resistance with excellent mechanical properties, 304L stainless steel is commonly used in the fabrication of spent nuclear fuel dry storage canisters. However, welds are sensitive to stress corrosion cracking (SCC) due to residual stress generation. Although SCC resistance can be improved by stress relieving the weld and changing the chloride environment, it is difficult to change corrosion environment for certain applications. Stress control in the weld can improve SCC resistance. Ultrasonic shot peening (USP) needs further research as compressive residual stresses and microstructure changes due to plastic deformation may play a role in improving SCC resistance. In this study, 304L stainless steel was welded to generate residual stresses and exposed to a chloride environment after USP treatment to improve SCC properties. Effects of USP on SCC resistance and crack growth of specimens with compressive residual stresses generated more than 1 mm from the surface were studied. In addition, correlations of compressive residual stress, grain size, intergranular corrosion properties, and pitting potential with crack propagation rate were determined and the improvement of SCC properties by USP was analyzed.

• The Journal of Internal Korean Medicine
• /
• v.32 no.4
• /
• pp.487-496
• /
• 2011

Objectives : The aim of this study was to investigate the hepatoprotective effect of Soshiho-tang (SSH) in mouse primary liver cells against hydrogen peroxide ($H_2O_2$)-induced oxidative stress. We also elucidated the molecular mechanism of hepatoprotective effect by SSH. Methods : Cell viability, level of ALT, AST and LDH, intracellular ROS level, mRNA expression and activity of antioxidant enzymes were used to evaluate hepatoprotection of SSH against $H_2O_2$. Target gene expressions were analyzed by real-time PCR. Results : Pre-treatment with SSH for 1 hour prevented cytotoxicity against $H_2O_2$. $H_2O_2$-induced ROS level decreased under SSH pre-treatment. mRNA expression of GPx and SOD increased in SSH-treated cells. In addition, HSP72 and HSP40 gene expression were elevated under SSH-treatment. Conclusions : These results indicate that SSH protects mouse primary liver cells from $H_2O_2$-induced oxidative injury. This hepatoprotective activity of SSH is mediated by decreasing intracellular ROS and increasing antioxidant enzyme expression (GPx and SOD) and stress response protein (HSP72 and HSP40).

• Journal of Korean Academy of Nursing Administration
• /
• v.12 no.2
• /
• pp.276-286
• /
• 2006

Purpose: The purpose of this study was to analyze the effects of self-efficacy and collective-efficacy on job stress in the nursing staff. Method: This study surveyed 160 nurses in three general hospitals in the Seoul and Gyung-gi province for two months starting in September 2004. The questionnaire consisted of 54 questions about job stress, 10 questions about self-efficacy, and 7 questions about collective-efficacy. The answers were on a scale rating of 5. The answer sheets were analyzed with descriptive statistics, the t-test, ANOVA, the tukey test, the Pearson correlation coefficients and stepwise multiple regression using SAS version 8.2. Result: The average job stress rating of the nurses was 3.11. The average self-efficacy and the average collective-efficacy were 3.41 and 3.39, respectively. The age, working department, income level, shift-work and hospital have influence on job stress. Efficacies such that self-efficacy and collective-efficacy have influence on job stress. The much efficacy makes the less job stress. The stepwise multiple regression revealed that the significant predictor of job stress was working department and hospital. Conclusion: This study showed that collective-efficacy as well as self-efficacy reduces job stress, so nursing intervention methods should promote collective-efficacy. The collective-efficacy improvement program should be developed to improve job performance, to improve cohesion of nursing units, and to improve satisfaction on the job. The next research could be to develop collective-efficacy improvement programs for nursing units.

• 종축개량
• /
• v.15 no.1
• /
• pp.39-43
• /
• 1993