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Effects of Soshiho-tang on Hydrogen Peroxide-induced Oxidative Damage in Hepatocytes  

Seo, Sang-Hee (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM))
Oh, Su-Young (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM))
Lee, Ji-Seon (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM))
Cho, Won-Kyung (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM))
Kim, Tae-Soo (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM))
Ma, Jin-Yeul (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM))
Publication Information
The Journal of Internal Korean Medicine / v.32, no.4, 2011 , pp. 487-496 More about this Journal
Abstract
Objectives : The aim of this study was to investigate the hepatoprotective effect of Soshiho-tang (SSH) in mouse primary liver cells against hydrogen peroxide ($H_2O_2$)-induced oxidative stress. We also elucidated the molecular mechanism of hepatoprotective effect by SSH. Methods : Cell viability, level of ALT, AST and LDH, intracellular ROS level, mRNA expression and activity of antioxidant enzymes were used to evaluate hepatoprotection of SSH against $H_2O_2$. Target gene expressions were analyzed by real-time PCR. Results : Pre-treatment with SSH for 1 hour prevented cytotoxicity against $H_2O_2$. $H_2O_2$-induced ROS level decreased under SSH pre-treatment. mRNA expression of GPx and SOD increased in SSH-treated cells. In addition, HSP72 and HSP40 gene expression were elevated under SSH-treatment. Conclusions : These results indicate that SSH protects mouse primary liver cells from $H_2O_2$-induced oxidative injury. This hepatoprotective activity of SSH is mediated by decreasing intracellular ROS and increasing antioxidant enzyme expression (GPx and SOD) and stress response protein (HSP72 and HSP40).
Keywords
Soshiho-tang; hepatoprotective; oxidative stress; mouse primary liver cell;
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Times Cited By KSCI : 3  (Citation Analysis)
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