• Journal of Korean Society of Steel Construction
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• v.21 no.6
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• pp.597-608
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• 2009

In this paper, large-deformation elasto-plastic analysis of space frames that considersjoint connection properties is presented. This method is based on the large-deformation formula with finite rotation, which was developed initially for elastic systems, and is extended herein to include the elasto-plastic effect and the member joint connection properties of semi-rigid what?. The analytical method was derived from the Eulerian concept, which takes into consideration the effects of large joint translations and rotations. The localmember force-deformation relationships were obtained from the beam-column approach, and the change caused by the axial strain in the member chord lengths and flexural bowing were taken into account. The effect of the axial force of the member on bending and torsional stiffness, and on the plastic moment capacity, is included in the analysis. The material is assumed to be ideally elasto-plastic, and yielding is considered concentrated at the member ends in the form of plastic hinges. The semi-rigid properties of the member joint connection are considered based on the power or linear model. The arc length method is usedto trace the post-buckling range of the elastic and elasto-plastic problems with the semi-rigid connection. A sample non-linear buckling analysis was carried out with the proposed space frame formulations to demonstrate the potential of the developed method in terms of its accuracy and efficiency.

• Journal of Korean Society of Steel Construction
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• v.22 no.3
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• pp.271-280
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• 2010

This study was programmed to fabricate a beam-to-column connection that is limited to a steel-welded moment connection with full-scale members, using SN steel. A cyclic seismic test was conducted of the nine specimens that were fabricated by choosing the test variable for the weld access hole geometry, connection design method, and RBS. From the test results, failure modes, the moment-drift behavior, and the strain distribution were provided. From the specimen material properties, the beam's nominal plastic flexural capacity and classified qualified connection as a special moment flame were calculated. By analyzing the skeleton part and the baushinger part, a range of strength-raising effects, and deformation ratios were provided, with which the seismic performance of the specimens were evaluated. The test results showed that the specimens eliminated their weld access holes that demonstrated higher seismic performance than the specimens' existing weld access holes, and that the WUF-W connection that was reinforced by the supplemental fillet weld around the shear tap that was fastened by five bolts demonstrated superior seismic performance.

• Journal of Mushroom
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• v.13 no.3
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• pp.228-232
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• 2015

'Nongjin-go' is a new breed strain of Lentinula edodes, saw-dust bag variety. It is a cross combination of dikaryon Lentinula edodes ASI 3305mut and monokaryon L5-16 of L. edodes ASI 3305(Sanjo701ho). We crossbred them by 2011 and verified productive capacity from 2012 to 2013 in Rural Development Administration. Optimum temperature of mycelial growth is $30^{\circ}C$ and it of fruit-body primodium formation is range from 15 to $23^{\circ}C$. Nongjigo is agricultured at mid-high temperature well. Fruiting body is platy-hemisphere, light brown and centralizing. And bast is formed around edge of pileus. Yield productions per period is regular than 'Sanjo701'. Plastic bag culture medium is 1.5 kilogram and culture periods are 90~100 days. As its browning of pileus in culture is a little slow, Light and ventilation is needed a lot in light-culturing. Humidity is controlled properly for its color in fruit-body growing. Tested culture medium is consisted of 80% Oak-Tree saw-dust and 20% rice-bran.

• Korean Journal of Microbiology
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• v.42 no.4
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• pp.326-331
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• 2006

In the present study, we examined biosorption characteristics of heavy metals onto the extracellular polysaccharide (EPS) produced by the purple nonsulfur photosynthetic bacteria Rhodopseudomonas sp. KH4, which was isolated from a stream in Anyang, Kyonggi-Do. When Cd (100 mg/L) and Cu (100 mg/L) were added to EPS (1.0 g/L) in the optimal condition (Cd; pH 8, Cu; pH 5, $40^{\circ}C$), 84.2 mg/L of Cd and 70.0 mg/L of Cu were adsorbed within 30 min and 10 min, respectively. When 100 mg/L of Cd and Cu were present as mixture, 16.8 mg/L of Cd and 48.7 mg/L of Cu were adsorbed at $25^{\circ}C$, pH 5. The maximum adsorption capacity determined by fitting Langmuir isotherms model was suitable for describing the biosorption of Cd (76.9 mg/g) and Cu (67.1 mg/g) by EPS. The neutral monosaccharide in the EPS determined by GC consisted of arabinose (2.4%), glucose (7.1%) and mannose (90.5%).

• Journal of Nutrition and Health
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• v.41 no.2
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• pp.119-126
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• 2008

This study was conducted to investigate the effect of soy protein hydrolyzate on lipid metabolism and antioxidant activity in the rat. Thirty-eight male rats of Sprague-Dawley strain were divided into five groups: casein, isolated soy protein (ISP), seoritae protein hydrolyzate (SH), soluble soy protein hydrolyzate (SS), and insoluble soy protein hydrolyzate (IS). The control diet (casein group) contained 20% casein protein and experimental diet contained 10% casein and 10% isolated soy-protein or soy-protein hydrolyzate. Fecal lipid content was increased and lipid apparent absorption rate was decreased significantly by the ISP group at the first week of experimental period. Blood triglyceride, total cholesterol, LDL-cholesterol and atherogenic index (AI) were decreased by soy protein hydrolyzate groups than casein group. Liver total lipid, triglyceride and cholesterol were not different among groups, but showed decreasing tendencies in soyprotein hydrolyzate groups. The lipid lowering effect was prominent in the IS group among soy protein hydrolyzate groups. Total antioxidant activity showed increasing tendency in the seoritae hydrolyzate group. Liver superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase activities also showed higher tendencies in the seoritae hydrolyzate group than other groups. In conclusion, insoluble soyprotein hydrolyzate was more effective in lowering body lipids and seoritae hydrolyzate had higher antioxidant capacity among soy protein hydrolyzates.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.4
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• pp.100-107
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• 2018

The objective of this study is to assess the strengthening effects of fiber reinforced polymer(FRP) sheets such as Carbon fiber, Glass fiber, and PET(polyethylene terephthalate) on reinforced concrete flexural members. Variables of theoretical analysis are types of strengthening materials, material properties and amount of strengthening materials. A virtual flexural member without FRP sheets was created as a control specimen to understand the structural behavior of the non-strengthened specimen in terms of elastic and ultimate cross section. In total, 11 specimens including one non-strengthened and ten strengthened specimens were investigated. Various variables such as types of strengthening, strengthening properties, and amount of strengthening were studied to compare the behavior of the control specimen with those of strengthened specimens with regard to moment-curvature relationship. Results of theoretical analysis showed that the moment capacity of strengthened specimens was superior to that of the control specimen. However, the control specimen indicated the best ductility among all the specimens. As the amount of strengthening increased, flexural performance was improved. Furthermore, the results indicated that the ductile effect of members was affected by the ultimate strain of FRP sheets. The strengthening effect on the damaged member was similar to that on the non-damaged one since there was less than 10% difference in terms of flexural strength and ductility. Therefore, even if a damaged member is treated as non-damaged for analysis there is probably no noticeable difference.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.7
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• pp.1009-1016
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• 2014

This study investigated the probiotic and functional characteristics of Mukeunji starter, Lactobacillus curvatus ML17, isolated from Mukeunji. Lb. curvatus ML17 was confirmed as a safe microorganism due to its non-hemolytic activity and non-production of harmful ${\beta}$-glucuronidase and ${\beta}$-glucosidase. Tolerance to artificial gastric and bile juice of Lb. curvatus ML17 was investigated. After incubation in artificial gastric and bile juice, the number of surviving cells was $1.38{\times}10^8$ CFU/mL. According to the results of adhesion assay, this strain also exhibited good adhesion to Caco-2 cells. Lb. curvatus ML17 showed good antimicrobial activity against food borne pathogens, especially Micrococcus luteus, Bacillus cereus, Salmonella enterica subsp. enterica, and Pseudomonas aeruginosa. Cell-free extract of Lb. curvatus ML17 exhibited high levels of DPPH scavenging capacity and inhibitory effects on growth of AGS human gastric adenocarcinoma cells and HT-29 human colon carcinoma cells. These results suggest that Lb. curvatus ML17 has potential for application in functional foods.

• Journal of Aquaculture
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• v.19 no.3
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• pp.166-172
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• 2006

Induced androgenesis, a form of artificial parthenogenesis is an important tool for the generation and use of genetically isogenic or clonal fish strain. An optimized protocol for the genetic inactivation of mud loach (Misgurnus mizolepis) oocytes (i.e. production of androgenetic haploid) was developed using UV-irradiation. Various dose levels of UV significantly affected the fertilizing capacity of the eggs, hatchability of embryos and incidence of haploidy. Based on the extensive examinations of treatment conditions on embryo viability and haploid incidence, the optimum dose level of UV irradiation was turned out to be $10,800ergs/mm^2$ with 56.9% of hatching success and 94.6% of haploidy. The overall yield of putative androgen under optimized treatment condition was more than 50% out of total eggs inseminated. The success of androgenetic reproduction of haploid genome was verified by flow cytometry and PCR amplification of transgene that is exclusive to either one of parental sexes. However, a small portion $(8\sim11%)$ of presumed androgenetic haploid larvae was proven to contain residual DNA fragment(s) from maternal parent.

• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.39-45
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• 2016

Pink Rot on melon and White Stain Symptom on grape are caused by Trichothecium roseum, one of the most important diseases of grape and melon. These diseases have been occurred in national-wide in Korea and causes irreversible damage on the grape and the melon at harvest season. This research presents the evaluation of the capacity of Bacillus subtillis HK2 to protect both melon and grape against T. reseum and establishes its role as a biocontrol agent. In this study, we isolated a Bacillus strain HK2 from rhizosphere soil, identified it as Bacillus subtillis by 16S rRNA analysis and demonstrated its antifungal activity against T. roseum. Under I-plate assay it was observed that the effect of hyphal growth inhibition was not due to production of volatile compounds. The optimum culture condition of HK2 was found at 30℃ and initial pH of 7.0. Application of HK2 culture suspension reduced 90.2% of white stain symptom on grape as compared to control, resulting in greater protection to grape against T. roseum infestation. Butanol extract of HK2 culture purified using flash column chromatography. The antifungal material was a polar substance as it showed antifungal activity in polar elute. Therefore, our results indicated a clear potential of B. subtilis HK2 to be used for biocontrol of Pink rot in melon and white stain symptom on grape caused by T. roseum.

• Journal of the Earthquake Engineering Society of Korea
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• v.13 no.1
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• pp.35-43
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• 2009

Two types of piloti-type high-rise RC building structures having irregularity in the lower two stories were selected as prototypes, and nonlinear time history analysis was performed using OpenSees to verify the analysis technique and to investigate the seismic capacity of those buildings. One of the buildings studied had a symmetrical moment-resisting frame (BF), while the other had an infilled shear wall in only one of the exterior frames (ESW). A fiber model, consisting of concrete and reinforcing bar represented from the stress-strain relationship, was adapted and used to simulate the nonlinearity of members, and MVLEM (Multi Vertical Linear Element Model) was used to simulate the behavior of the wall. The analytical results simulate the behavior of piloti-type high-rise RC building structures well, including the stiffness and yield force of piloti stories, the rocking behavior of the upper structure and the variation of the axial stiffness of the column due to variation in loading condition. However, MVLEM has a limitation in simulating the abrupt increasing lateral stiffness of a wall, due to the torsional mode behavior of the building. The design force obtained from a nonlinear time history analysis was shown to be about $20{\sim}30%$ smaller than that obtained in the experiment. For this reason, further research is required to match the analytical results with real structures, in order to use nonlinear time history analysis in designing a piloti-type high-rise RC building.