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http://dx.doi.org/10.3746/jkfn.2014.43.7.1009

Characterization of Probiotic and Functional Properties of Lactobacillus curvatus ML17, a Mukeunji Starter  

Kim, Hyo Ju (Food Research Institute, Jeonnam Bioindustry Foundation)
Sung, Hea Mi (Food Research Institute, Jeonnam Bioindustry Foundation)
Shin, Hyun-Kyung (Food Research Institute, Jeonnam Bioindustry Foundation)
Kim, Ki Myong (Dept. of Food and Nutrition, Honam University)
Yang, Eun Ju (Food Research Institute, Jeonnam Bioindustry Foundation)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.43, no.7, 2014 , pp. 1009-1016 More about this Journal
Abstract
This study investigated the probiotic and functional characteristics of Mukeunji starter, Lactobacillus curvatus ML17, isolated from Mukeunji. Lb. curvatus ML17 was confirmed as a safe microorganism due to its non-hemolytic activity and non-production of harmful ${\beta}$-glucuronidase and ${\beta}$-glucosidase. Tolerance to artificial gastric and bile juice of Lb. curvatus ML17 was investigated. After incubation in artificial gastric and bile juice, the number of surviving cells was $1.38{\times}10^8$ CFU/mL. According to the results of adhesion assay, this strain also exhibited good adhesion to Caco-2 cells. Lb. curvatus ML17 showed good antimicrobial activity against food borne pathogens, especially Micrococcus luteus, Bacillus cereus, Salmonella enterica subsp. enterica, and Pseudomonas aeruginosa. Cell-free extract of Lb. curvatus ML17 exhibited high levels of DPPH scavenging capacity and inhibitory effects on growth of AGS human gastric adenocarcinoma cells and HT-29 human colon carcinoma cells. These results suggest that Lb. curvatus ML17 has potential for application in functional foods.
Keywords
Mukeunji; Lactobacillus curvatus; adhesion to Caco-2; antimicrobial activity; anticancer activity;
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Times Cited By KSCI : 21  (Citation Analysis)
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1 Yu JJ, Park HJ, Kim SG, Oh SH. 2009. Isolation, identification, and characterization of Weissella strains with high ornithine producing capacity from kimchi. Korean J Microbiol 45: 339-345.   과학기술학회마을
2 Sandire WE, Muralidhara KS, Elliker PR, England DC. 1972. Lactic acid bacteria in food and health; a review with special reference to enteropathogenic Escherichia coli as well as certain enteric diseases and their treatment with antibiotics and lactobacilli. J Milk Food Technol 35: 691-702.   DOI
3 Chung HJ, Kim HR, Yoo MJ. 2005. Changes in texture and sensory properties of low-temperature and long-term fermented Baechu kimchi during the fermentation. Korean J Food Culture 20: 426-432.   과학기술학회마을
4 Lee KH, Park JY, Jeong SJ, Kwon GH, Lee HJ, Chang HC, Chung DK, Lee JH, Kim JH. 2007. Characterization of paraplantaricin C7, a novel bacteriocin produced by Lactobacillus paraplantarum C7 isolated from kimchi. J Microbiol Biotechnol 17: 287-296.   과학기술학회마을
5 Kang MR, Kim DR, Kim TW, Park SH, Kim HJ, Jang JY, Han ES. 2012. Selection of probiotic bacteria from Yulmoo Kimchi using a stimulated human intestinal model system. J Korean Soc Food Sci Nutr 41: 396-401.   과학기술학회마을   DOI   ScienceOn
6 Lim SM. 2010. Resistance to reactive oxygen species and antioxidant activities of some strains of lactic acid bacteria from the mustard leaf kimchi. Korean J Microbiol 46: 375-382.   과학기술학회마을
7 Lee IH, Lee SH, Lee IS, Park YK, Chung DK, Choue R. 2008. Effects of probiotic extracts of kimchi on immune function in NC/Nga mice. Korean J Food Sci Technol 40: 82-87.   과학기술학회마을
8 Shin K, Chae O, Park I, Hong S, Choe T. 1998. Antitumor effects of mice fed with cell lysate of Lactobacillus plantarum isolated from Kimchi. Korean J Biotechnol Bioeng 13: 357-363.   과학기술학회마을
9 Yu MH, Im HG, Im NK, Hwang EY, Choi JH, Lee EJ, Kim JB, Lee IS, Seo HJ. 2009. Anti-hypertensive activities of Lactobacillus isolated from kimchi. Korean J Food Sci Technol 41: 428-434.   과학기술학회마을
10 Yoon JY, Jung KO, Kim SH, Park KY. 2004. Antiobesity effect of baek-kimchi (whitish baechu kimchi) in rats fed high fat diet. J Food Sci Nutr 9: 259-264.   과학기술학회마을   DOI
11 Kim HJ, Shin HK, Yang EJ. 2013. Production and fermentation characteristics of mukeunji with a mixed starter. J Korean Soc Food Sci Nutr 42: 1467-1474.   과학기술학회마을   DOI
12 Ji SH, Han WC, Lee JC, Cheong C, Kang SA, Lee JH, Jang KH. 2009. Effect of low temperature on the qualities of long-term fermented kimchi (Korean pickled cabbage). Korean J Food Preserv 16: 804-809.   과학기술학회마을
13 Lee JJ, Jung HO, Lee MY, Chang HC. 2012. Characteristics of mandu with ripened Korean cabbage kimchi. Korean J Food Preserv 19: 209-215.   과학기술학회마을   DOI   ScienceOn
14 Kim SE, Kim YH, Lee H, Kim DO, Kim HY. 2012. Probiotic properties of lactic acid bacteria isolated from mukeunji, a long-term ripened kimchi. Food Sci Biotechnol 21: 1135-1140.   과학기술학회마을   DOI
15 Cederbaum AI, Lu Y, Wu D. 2009. Role of oxidative stress in alcohol-induced liver injury. Arch Toxicol 83: 519-548.   DOI   ScienceOn
16 Kobayashi Y, Tohyama K, Terashima T. 1974. Studies on biological characteristics of Lactobacillus. II. Tolerance of the multiple antibiotic resistance strain Lactobacillus casei PSR 3002 to artificial digestive fluids. Japan J Bacteriol 29: 691-697.   DOI   ScienceOn
17 Yang EJ, Chang HC. 2007. Characterization of bacteriocinlike substances produced by Bacillus subtilis MJP1. Kor J Microbiol Biotechnol 35: 339-346.
18 Nanno M, Morotomi M, Takayama H, Kuroshima T, Tanaka R, Mutai M. 1986. Mutagenic activation of biliary metabolites of benzo(a)pyrene by $\beta$-glucuronidase-positive bacteria in human faeces. J Med Micribiol 22: 351-355.   DOI
19 Brand-Williams W, Cufelier ME, Berset C. 1995. Use of free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 28: 25-30.   DOI
20 Hawksworth G, Drasar BS, Hill MJ. 1971. Intestinal bacteria and the hydrolysis of glycosidic bonds. J Med Microbiol 4: 451-459.   DOI
21 Cumming JH, Macfarlane GT. 1991. The control and consequences of bacterial fermentation in the human colon. J Appl Microbiol 70: 443-459.
22 Goldin BR. 1990. Intestinal microflora: metabolism of drugs and carcinogens. Ann Med 22: 43-48.   DOI
23 EFSA. 2007. Introduction of a qualified presumption of safety (QPS) approach for assessment of selected microorganisms referred to EFSA. EFSA J 587: 1-16.
24 Hebert EM, Saavedra L, Taranto MP, Mozzi F, Magni C, Nader ME, Font de Valdez G, Sesma F, Vignolo G, Raya RR. 2012. Genome sequence of the bacteriocin-producing Lactobacillus curvatus strain CRL705. J Bacteriol 194: 538-539.   DOI
25 Park DY, Ahn YT, Park SH, Huh CS, Yoo SR, Yu R, Sung MK, McGregor RA, Choi MS. 2013. Supplementation of Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 in diet-induced obese mice is associated with gut microbial changes and reduction in obesity. PLoS One 8: e59470.   DOI
26 Casaburi A, Di Monaco R, Cavella S, Toldrá F, Ercolini D, Villani F. 2008. Proteolytic and lipolytic starter cultures and their effect on traditional fermented sausages ripening and sensory traits. Food Microbiol 25: 335-347.   DOI
27 Lee NK, Kim HW, Choi SY, Paik HD. 2003. Some probiotic properties of some lactic acid bacteria and yeasts isolated from jeot-gal. Kor J Microbiol Biotechnol 31: 297-300.   과학기술학회마을
28 Castellano P, Gonzalez C, Carduza F, Vignolo G. 2010. Protective action of Lactobacillus curvatus CRL705 on vacuum- packaged raw beef. Effect on sensory and structural characteristics. Meat Sci 85: 394-401.   DOI
29 Cebrian R1, Banos A, Valdivia E, Perez-Pulido R, Martinez-Bueno M, Maqueda M. 2012. Characterization of functional, safety, and probiotic properties of Enterococcus faecalis UGRA10, a new AS-48-producer strain. Food Microbiol 30: 59-67.   DOI
30 Park CW, Youn M, Jung YM, Kim H, Jeong Y, Lee HK, Kim HO, Lee I, Lee SW, Kang KH, Park YH. 2008. New functional probiotic Lactobacillus sakei probio 65 alleviates atopic symptoms in the mouse. J Med Food 11: 405-412.   과학기술학회마을   DOI   ScienceOn
31 Ann YG. 2011. Probiotic lactic acid bacteria. Korean J Food & Nutr 24: 817-832.   과학기술학회마을   DOI   ScienceOn
32 Reis JA, Paula AT, Casarotti SN, Penna ALB. 2012. Lactic acid bacteria antimicrobial compounds: characteristics and applications. Food Eng Rev 4: 124-140.   DOI
33 Lehto EM, Salminen S. 1997. Adhesion of two Lactobacillus strains, one Lactococcus, and one Propionibacterium strain to cultured human intestinal Caco-2 cell line. Biosci Microflora 16: 13-17.   DOI
34 Lee J. 2005. Adhesion of kimchi Lactobacillus strains to Caco-2 cell membrane and sequestration of aflatoxin B1. J Korean Soc Food Sci Nutr 34: 581-585.   DOI
35 Shimamura S, Abe F, Ishibashi N, Miyakawa H, Yaeshima T, Araya T, Tomita M. 1992. Relationship between oxygen sensitivity and oxygen metabolism of Bifidobacterium species. J Dairy Sci 75: 3296-3306.   DOI   ScienceOn
36 Lim KS, Huh CS. 2006. Adhesion of bifidobacteria to Caco-2 cells and in relation to cell surface hydrophobicity. Korean J Food Sci Ani Resour 26: 497-502.   과학기술학회마을
37 Li S, Zhao Y, Zhang L, Zhang X, Huang L, Li D, Niu C, Yang Z, Wang Q. 2012. Antioxidant activity of Lactobacillus plantarum strains isolated from traditional Chinese fermented foods. Food Chem 135: 1914-1919.   DOI
38 Zitzelsberger W, Götz F, Schleifer KH. 1984. Distribution of superoxide dismutases, oxides, and NADH peroxides and various streptococci. FEMS Microbiol Lett 21: 243-246.   DOI   ScienceOn
39 Kim HS, Ham JS. 2003. Antioxidative ability of lactic acid bacteria. Korean J Food Sci Ani Resour 23: 186-192.   과학기술학회마을
40 Kato I, Kobayashi S, Yokokura T, Mutai M. 1981. Antitumor activity of Lactobacillus casei in mice. Gann 72: 517-523.
41 Friend BA, Shahani KM. 1984. Antitumor properties of lactobacilli and dairy products fermented by lactobacilli. J Food Prot 47: 717-723.   DOI
42 Yeo MH, Kim DM, Kim YH, Kim JH, Baek H, Chung MJ. 2008. Antitumor activity of CBT-AK5 purified from Lactobacillus casei against Sarcoma-180 infected ICR mice. Korean J Dairy Sci Technol 26: 23-30.   과학기술학회마을
43 Gilliland SE, Staley TE, Bush LJ. 1984. Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct. J Dairy Sci 67: 3045-3051.   DOI   ScienceOn
44 Wang K, Li W, Rui X, Chen X, Jiang M, Dong M. 2014. Characterization of a novel exopolysaccharide with antitumor activity from Lactobacillus plantarum 70810. Int J Biol Macromol 63: 133-139.   DOI
45 Uccello M, Malaguarnera G, Basile F, D'agata V, Malaguarnera M, Bertino G, Vacante M, Drago F, Biondi A. 2012. Potential role of probiotics on colorectal cancer prevention. BMC Surgery 12(Suppl 1): S35.
46 Kim DS, Cho HW, Kim DH, Oh KH. 2013. Functional characterization of Lactobacillus sakei JK-17 isolated from longterm fermented Kimchi, Muk Eun Ji. Korean J Biotechnol Bioeng 28: 18-23.
47 Hong HJ, Kim E, Cho D, Kim TS. 2010. Differential suppression of heat-killed lactobacilli isolated from kimchi, a Korean traditional food, on airway hyper-responsiveness in mice. J Clin Immunol 30: 449-458.   DOI   ScienceOn