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Intraspecies Androgenesis in Mud Loach (Misgurnus mizolepis): I. Optimization of the Egg Inactivation and Haploid Androgenesis Using Transgene Marker  

Nam Yoon-Kwon (Department of Aquaculture & Institute of Marine Living Modified Organisms (iMLMO), Pukyong Notional University)
Noh Choong-Hwan (Marine Resources and Research Department, Korea Ocean Research and Development Institute)
Kim Dong-Soo (Department of Aquaculture & Institute of Marine Living Modified Organisms (iMLMO), Pukyong Notional University)
Publication Information
Journal of Aquaculture / v.19, no.3, 2006 , pp. 166-172 More about this Journal
Abstract
Induced androgenesis, a form of artificial parthenogenesis is an important tool for the generation and use of genetically isogenic or clonal fish strain. An optimized protocol for the genetic inactivation of mud loach (Misgurnus mizolepis) oocytes (i.e. production of androgenetic haploid) was developed using UV-irradiation. Various dose levels of UV significantly affected the fertilizing capacity of the eggs, hatchability of embryos and incidence of haploidy. Based on the extensive examinations of treatment conditions on embryo viability and haploid incidence, the optimum dose level of UV irradiation was turned out to be $10,800ergs/mm^2$ with 56.9% of hatching success and 94.6% of haploidy. The overall yield of putative androgen under optimized treatment condition was more than 50% out of total eggs inseminated. The success of androgenetic reproduction of haploid genome was verified by flow cytometry and PCR amplification of transgene that is exclusive to either one of parental sexes. However, a small portion $(8\sim11%)$ of presumed androgenetic haploid larvae was proven to contain residual DNA fragment(s) from maternal parent.
Keywords
Induced androgenesis; Mud loach; Haploidy induction; Transgene marker;
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