• Food Engineering Progress
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• v.15 no.2
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• pp.130-135
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• 2011

Acanthopanax sessiliflorus (A. sessiliflorus) has been known as a traditional medicine having anti-stress, antioxidative and platelet aggregation inhibitory effects. This study was undertaken to investigate the functional properties of water extracts from four parts of A. sessiliflorus. Root, stem, leaf and fruit extracts from A. sessiliflorus were prepared with hot water ($80^{\circ}C$). The contents of functional substances, eleutheroside B and E, polyphenol, antioxidative activity, nitrite scavenging ability and anti-cancer activity of the extracts were determined. The contents of eleutheroside E in stem, root and fruit extracts were 542.50 ${\mu}$g/g, 343.35 ${\mu}$g/g and 30.78 ${\mu}$g/g, respectively. A large part of eleutheroside B was found in fruit (372.01 ${\mu}$g/g) and root (289.33 ${\mu}$g/g) extracts. Root and stem extracts contained 227.21 mg/100g and 131.22 mg/100g of polyphenols, respectively. Antioxidative activities (electron donating ability) of stem and root extracts were 79.87% and 77.27%, respectively. It appears that the antioxidative activities were related to polyphenol contents of the extracts. Most extracts showed 76-81.5% of nitrite scavenging ability at pH 1.2. It reveals that water extract from parts of A. sessiliflorus can inhibit formation of nitrosoamine in food. Effects of the extracts on the growth of normal and cancer cell lines were investigated. Extracts showed no cytotoxicity to normal dendritic cell line (DC2.4). Especially, the root extract promoted the growth of normal cell line. Root and stem extracts had 20-23% of inhibitory effect against stomach cancer cell line (SNU-719) and liver cancer cell line (Hep3B). These result indicated that the extracts from A. sessiliflorus can be used as functional food materials with antioxidative activity and nitrite scavenging ability to eliminate nitrosoamine in food.

• Journal of Aquaculture
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• v.21 no.4
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• pp.278-284
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• 2008

Artificially-produced juvenile black sea bream Acanthopagrus schlegeli (total length $8.3{\pm}1.0\;cm$, body weight $11.2{\pm}4.2\;g$) were released in eelgrass, Zostera marina bed and their initial food organism and changes of body compositions were investigated for one month after release. Predation rates in fish sampled on 1st and 3rd days were 79%, increased up to 100% on 7th and 10th days, and then decreased on 30th days as 75%. Major prey organisms was composed of mainly Amphipoda and Gastropoda. Ratio of Amphipoda and Gastropoda in stomach were highest in fish sampled on 15th and 1st days after release, respectively. Crustacea and Algae were maintained about 20% during a sampling period. Visceral weight index (VWI) offish sampled 20th and 30th after release were significantly higher than that of initial. Carcass crude protein and lipid contents of released fish were showed significantly decreasing; however carcass n-3 HUFA composition was showed increasing tendency with the passage of time after release. Eelgrass bed was supposed to be helpful for the released fish to adjust their feeding habits and biochemical metabolism to the natural environment within a short period after release.

• Korean Journal of Environmental Biology
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• v.27 no.3
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• pp.297-305
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• 2009

The Asian paddle crab, Charybdis japonica (A. Milne-Edwards) is an important fishery resource in Korea. Despite its common occurrence and commercial importance, few studies have been carried out on the life cycle and trophic ecology of these populations inhabiting on the tidal flat. Food habits of C. japonica populations were investigated on the Jangbong tidal flat, Incheon, Korea. Monthly samples were taken using a modified otter trawl from November 1999 to January 2001. Based on the examination of stomach contents from 257 individuals, the frequencies of occurrence and the relative volumes of food items were analysed. As a result, the crustaceans (the brachyura, the cirripedia and the harpacticoid copepoda), the echinoderms (the ophiuroidea) and the molluscs (the cephalopoda Loligo beka, the bivalves Bivalvia unid. and Mytilus galloprovincialis) were important food for this species. To examine ontogenetic shifts in diets, individuals of C. japonica were categorized into 2 different size classes (${\leq}60mm$ and ${\geq}61mm$ in carapace width) representing sexually premature and mature stages. The food items of the small crabs (${\leq}60mm$) were dominated by the crustaceans with the harpacticoid copepods constituting 84.6% of total prey number. The next group was the bivalves and the brachyurans. On the other hand, the large crabs (${\geq}61mm$) mainly feed on the bivalvia (Mytilus galloprovincialis and Bivalvia unid.), the brachyurans (Crab unid.) and occasionally the cephalopods and fishes. The difference in proportions of the main prey items among these two size classes was statistically significant. Therefore, the feeding habits of the portunid crab, C. japonica changed with growth.

• Korean Journal of Environmental Biology
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• v.33 no.3
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• pp.338-344
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• 2015

In an effort to restore the amphibians in urban freshwater system the characteristics of feeding activity of Rana rugosa living in Gyeonggi-provinces (Yangpyeong-gun and Namyangju-si) was analyzed from 2013 to 2014. The stomach contents of R. rugosa was analyzed non-invasively and compared to organisms captured by sweeping and trapping in their habitat. As a result, Hymenoptera and Cleoptera were primarily preyed by R. rugosa. Particularly, the proportion of Formicidae was more than 98% among the preyed Hymenoptera. Trapped insects in the habitat of R. rugosa's in Namyangju were Hymenoptera (58%, Formicidae 99%), Collembola (17%), Orthoptera (10%) and Diptera (9%) in order in order. In Yangpyeong, trapped insects were Collembola (49%), Orthoptera (14%), Arachnida (9%), Diptera (9%), Cleoptera (7%) and Hymenoptera (3%). Even though Hemiptera and Diptera species are abundant in the streamside zones, R. rugosa could easily hunt Formicidae or Coleoptera on rocks or grassland at streamside. R. rugosa consume small sized ground-insects that are easily found rather than searching for the specific prey. Prey resource of urban stream may be not a limiting factor of R. rugosa inhabitation.

• Korean Journal of Ichthyology
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• v.27 no.2
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• pp.78-85
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• 2015

Population ecology of Rhodeus uyekii (Pisces: Acheilognathinae) was investigated at the Bongseocheon, Mankyeonggang (River) in Korea, from January to December 2014. It mainly inhabits the slow-flowing waters having mud bottoms. Measurement of the standard length of the population indicated that the following standardization: 24~29 mm are one year old, 30~35 mm are two years old, 36~39 mm are three years old, and >40 mm group are regarded over four years old (October in 2014). The spawning season was from March to June, with water temperatures in the range $11.5{\sim}27.0^{\circ}C$. The sex ratio of female : male was 1 : 0.77. The number of eggs in the ovaries was 104~168 ($mean{\pm}SD$: $124{\pm}18.1$). The size of the matured eggs was 2.8~3.5 ($3.2{\pm}0.15$) mm in the long axis, and 1.4~1.5 ($1.4{\pm}0.03$) mm in the short axis. The eggs were found in Unio douglasiae and the number of eggs, larvae or both was 3~18 ($10.2{\pm}5.0$). Stomach contents were mainly phytoplanktons such as the genera Navicular, Cocconeis, Cymbella (Bacilariophyceae) and Cloniophora (Chlorophyceae).

• The Journal of Internal Korean Medicine
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• v.30 no.2
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• pp.399-409
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• 2009

Objectives : The aim of this study was to experiment the antitumor activity of Agrimonia pilosa Ledebour (APL) in human stomach cancer (AGS) cell lines (in vitro) and male C57BL/6J mouse (in vivo). Methods : The effects of the ethanol extract from the plant on several transplantable rodent tumors were investigated in vitro by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy phenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay. DNA content analysis and Western blot analysis. Agrimonia pilosa Ledebour (APL) was given to rats with Lewis Lung Carcinoma (LLC) cells. The experimental rats were divided into 3 groups in vivo. Saline was injected into the abdominal cavity in the first group, 50 mg/kg APL was injected into the abdominal cavity in the second group and 100 mg/kg was injected into the abdominal cavity in the third group. After that, we checked their tumor volume periodically. Results : At first, human gastric cancer (AGS) cell lines (in vitro) showed decreased cell viability, and increased $sub-G_1$ contents. When we experimented rat intestinal epithelial (RIE)l as same condition, this result didn't show. With this, compared to normal cells, Agrimonia pilosa Ledebour (APL) led selectively to the extinction of cells only in human gastric cancer. Moreover, we showed that the traditional herbal medicine APL induced caspase-dependent apoptosis in AGS cells. Next, APL inhibited the growth of LLC-bearing mouse tumor. However, we could not verify APL induced caspase-dependent apoptosis in LLC-bearing mouse tumor. Conclusions : The roots of Agrimonia pilosa Ledebour (APL) contain some antitumor constituents.

• Korean Journal of Ichthyology
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• v.7 no.1
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• pp.56-63
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• 1995

Ecological study of the Cynoglossus joyneri was conducted based on the specimens from the western coast of Korea from 1992 to 1994. Ecological characteristics of this species such as gonadal development, occurrence of larvae, stomach contents, and environmental conditions were investigated. Few larvae of Cynoglossus joyneri occurred in Kunsan coast. This coast is influenced by the freshwater input of the Mangyong and Kum River, especially during the summer. Therefore, the larvae of C. joyneri were considered to be spawned and grown during their early life stage in the high saline water in outer bay. Spawning occur from June to September, having the peak spawning period from July to August. Biological minimum size of matured fishes containing mature oocytes is about 143.5mm in total length, which requires about 20 months after hatching. The ranges of total length by age are as follows : 0-ring group(floating stage), 30~70mm ; 1-ring group, 49.0~133.0mm 2-ring group, 128.6~167.0mm ; 3-ring group, 169.0~202.0mm ; 4-ring group, 200.7~240.0mm. The major food items of young fish under 8cm were copepods and invertebrate larvae, and those of adult fishes were annelids(polychaets) in winter, and bivalves and shrimps in summer and autumn. Therefore, it can be assumed that the population of C. joyneri could be reduced due to the decrease of food organisms caused by the continuous reclamation activities in the inner bays of the west coast of Korea.

• Journal of Aquaculture
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• v.20 no.2
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• pp.90-95
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• 2007

Artificially-produced juvenile black sea bream, Acanthopagrus schlegeli (total length $10.4{\pm}1.1\;cm$, body weight $18.7{\pm}3.4\;g$) were released into the Gamak Bay in Korea. And their initial feeding habits and body compositions were compared with those of cultured fish (CUL) for a period of one month after release. The released black sea bream commenced feeding on the 1st day after release, and predation rate was more than 80% during the sampling period. The stomach content of fish was composed of mainly fishing bait and krill in fish sampled on 15th days after release; however, its content changed to the natural organisms such as algae and small crustaceans when the fish was sampled on 20th days after release. Crude lipid content of carcass of fish in the sampling station A (SA) were lower than that of fish in the initial, sampling station B (SB) and CUL for the 5th and 10th days after release. Carcass n-3 HUFA composition, total amino acid and essential amino acid contents of released fish showed high trend compared to that of cultured fish in 30th days after release. The released fish have the ability to adjust their feeding habits and biochemical metabolism to the natural environment within a short period even though they showed preference to artificial feed in the early days after release.

• The Journal of Engineering Geology
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• v.10 no.1
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• pp.51-62
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• 2000

The $CO_2$-richrich water pumps or springs out at two sites (Sinchon and Kohran) consisting of Cretaceous sedimentary rocks in Kyungpook area. The water has been long known as its soda pop-liketaste and therapeutic effect against calcium deficit, stomach and skin troubles, etc. The water arecharacterized by a high $CO_2$ concentration $(P_{CO2}=0.29~l.01 atm)$ and electrical conductance (1,093~2,810$\mu$S/cm). The $CO_2$-rich water belongs to Ca(Na)-$HCO_3$ type in chemical classification. The contents of Ca, Mg, Na, HCO$_3$ and Fe of $CO_2$-rich water show much higher values than those of general groundwater Environmental isotopic data $(^2H/^1H, ^{18}O/^{16}O and ^3H/^1H)$ indicate that the water is ofmeteoric origin recharged after 1950s. The $CO_2$ in the springs seems to be originated from deep-seatedsource related to acidic porphyry and granite nearby sedimentary rocks. Carbonate minerals and albiteare likely to be the major source minerals of the dissoved inorganic constituents in the $CO_2$-rich water.The equilibrium state between major minerals and $CO_2$-rich water was calculated by a thermodynamicprogram.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.6
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• pp.462-468
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• 2004

In order to evaluate adaptation to the environment in released fish, digestive organ and body composition were compared among 1-year old cultured (CUL), released (REL) and wild (WIL) Black Sea Bream, Acanthopagrus schlegeli. Hepatosomatic index (HSI) of the REL was significantly lower than those of the CUL and WIL fish, but intestine weight index (IWI) of REL was significantly higher than those of the CUL and WIL fish. There were no significant differences in stomach weight index (SWI) and condition facto. (CF) among those different fish groups. The number of pyloric caeca of the REL and WIL fish were 4, but the $30{\%}$ of CUL fish was 3 counts. The moisture content of whole body in the WIL fish was significantly higher than the CUL and REL fish, but significant lowest in the crude lipid of whole body. The content of moisture and crude protein of dorsal muscle were no significant differences among the CUL, REL and WIL fish, but crude lipid content of WIL fish was significantly lower than the CUL and REL fish. Moisture content of CUL fish in the liver was significantly lower than the REL and WIL fish, and crude lipid was significant lowest in the WIL fish. Amino acid content of dorsal muscle in the WIL fish was highest in the total amino acid, EAA and E/A ratio, and CUL fish was highest EAA and E/A ratio in liver. EPA content in dorsal muscle of WIL fish was lower than CUL and REL fish, and the REL fish was highest in DHA content of the fatty acid. EPA content in liver of WIL fish was lower than CUL and REL fish, and DHA content in REL and WIL fish was highest and lowest. Crude protein content of CUL and WIL fish in the scale was significantly higher than REL fish, but there were no significant differences in contents of crude lipid and ash. Ca and P of scale were formed most of mineral and content of P in CUL fish was significantly higher than those of REL and WIL fish. There were no significant differences in Mg, K, Na and S of scale, but CUL fish was significantly lower than REL fish in C1. The results suggest that difference of digestive organs and body composition concluded it from differences of inhabitation environment and feed formulations.