• The Korean Journal of Mycology
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• v.39 no.3
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• pp.175-179
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• 2011

Light wavelength is the major factor of fruit body development associated with mushroom cultivation, but its wavelength range in Pleurotus eryngii is poorly understood. Using four kinds of light emitting diode (LED) including blue (475 nm), green (525 nm), yellowed (590 nm) and red (660 nm), we investigated to elucidate suitable light wavelength during primordium formation and fruit body development of P. eryngii on bottle cultivation. Primordia formation did not occur in blue light and red light. The morphological properties of fruit body in fluorescent lamp and blue light irradiation were showed thicker and larger pileus than those in other LEDs. However, length of stipe in fluorescent lamp and blue light was shorter than that of other LEDs. The DPPH radical was high in blue light, green light, and yellow light except for red light, and the polyphenol was high in four kinds of LED sources. And ergosterol was the highest in the green light. Thus, the high-quality mushroom production of P. eryngii is possible to green light condition considering productivity and functional materials.

• The Korean Journal of Mycology
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• v.39 no.3
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• pp.231-234
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• 2011

This study was performed to develop new Hypsizygus marmoreus cultivars that have enhanced functional materials and improved physiological characteristics with mutagenesis by gamma ray irradiation. Protoplasts of H. marmoreus brown strain HYM-056 were irradiated by gamma ray for mutagenesis, and then 2,000 clones of mutants were randomly selected and the fruiting bodies were induced by bottle culture. Among them, 157 isolates with fast-growing, heavy and many fruiting body-producing were selected. The isolates were cultured in plastic bottle containing rice bran, barley hulls and fir sawdust to form the fruiting bodies. About 100 days after inoculation, characteristic of fruiting bodies were investigated. The isolates were divided into 6 groups based on color, shape and size of pileus, and length, diameter, number and weight of stipe. In addition, the genetic variation of the isolates was analyzed by URP-PCR fingerprinting.

• Journal of the Korean Wood Science and Technology
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• v.29 no.4
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• pp.79-88
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• 2001

Lectin was isolated from shiitake mushroom (Lentinula edodes) with 0.15 M NaCl solution, and purified by the following procedures : precipitation by ammonium sulfate, anion exchange column chromatography on DEAE Sephadex A-50 and hydroxyapatite column chromatography. The fresh pileus part of the mushroom contained more than two times of lectin compared to the stipe part, and lectins and its activity were reduced by heating. The extraction yield of crude lectin was 46.03%, 28% yield after purification on on DEAE Sephadex A-50 column chromatography. Some amino acids, aspartic acid, serine, alanine and histidine, were increased by purification process. Relatively low molecular weight parts of lectin had the agglutinating activity for rabbit blood, and its molecular weight was about 23 kDa The molecular weights of purified lectins, LA-a and LB-b, by the hydroxyapatite column chromatography were 24 kDa and 23 kDa, respectively.

• Journal of Mushroom
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• v.3 no.4
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• pp.129-132
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• 2005

A new functional mushroom cultivated variety "Jangsaeng" of Fomitella fraxinea was developed by crossing monokaryons derived from collected strains for contribution to mushroom farmers. The major characteristics of this mushroom were compared to Yeongji 2ho of Ganoderma lucidum because of their morphological similarities. The optimal temperature of mycelial growth and fruitbody sprouting and growth were 30 and 25~30, respectively. The surface color of fruitbody pilus was reddish brown and the fruitbody was flat kidney-shaped like Ganoderma lucidum except that the stipe was absent. Its texture was so tough and rigid that the yield per 1,000 ml bottle might be as high as 136 g. The hot water extract of this medicinal mushroom is fit to drink just like Ganoderma lucidum but its taste is not bitter.

• Journal of Mushroom
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• v.9 no.3
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• pp.96-100
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• 2011

MST247ns(Hwaseong #2) was developed by the method of Di-mon mating between monokaryotic strains derived from "Hwaseong #1" and dikaryotic strain "Suhan #1". The optimum temperature of mycelial growth was $25-30^{\circ}C$. The optimum temperature of primordia formation and fruiting body development were $8-15^{\circ}C$ and $9-14^{\circ}C$. Days of primordia formation were 4-5 days later Suhan #1. The stipes were longer than "Suhan #1". The surfaces of stipe were white and the tissues got harder and more elastic. Therefore, the management of growth environment under low temperatures was relatively easy and storability got much better.

• Journal of Mushroom
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• v.6 no.3_4
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• pp.115-120
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• 2008

A new commercial strain 'Garlmoe' was developed by crossing monokaryons from selected wild strains. The optimal temperature of mycelial growth was $25^{\circ}C$ but it needed to adjust to $16{\sim}18^{\circ}C$ when incubated at the bottle cultivation. The optimal temperatures of fruiting body initiation and development were almost same to others like as $14^{\circ}C$ and $7{\sim}10^{\circ}C$, respectively. The color of 'Garlmoe' was typical to wild winter mushroom, that is, pileus was brown and lower part of stipe was dark brown. The surface on pileus of 'Garlmoe' was sticky 'Garlmoe' was a good commercial strain with high quality and high productivity characterized as quite even budding habit, straight and strong stipes and convex type pilei. The taste of this variety was very excellent because of better chewing feeling than white ones.

• The Korean Journal of Mycology
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• v.26 no.3 s.86
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• pp.314-320
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• 1998

A 0.4 kb cDNA fragment was isolated from mRNA of UV or mechanical wound damaged Pleurotus sajor-caju by the differential display method. Expression of the gene corresponding to this cDNA fragment was highly induced by mechanical wound damage or UV treatment. This gene showed only basal level expression in mycelia, stipe, and cap under normal growth conditions. Sequencing analysis showed that this cDNA fragment contains partial open reading frame. Homology search using genbank database revealed that although this gene do not have homology with already reported wound induced genes, it has a significant sequence homology in defined region with the cdc2-related protein kinase gene which is known to be involved in negative regulation of meiotic maturation in Xenopus oocytes.

• The Korean Journal of Mycology
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• v.49 no.1
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• pp.45-55
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• 2021

Artomyces microspora is a genus of coral fungi from the family Auriscalpiaceae that have sporophores which are clavarioid, profusely and pyxidately branched, and devoid of a conspicuous stipe. These fungi can be found in summer and fall. This study aimed to decipher fundamental information regarding optimal growth conditions of Artomyces microsporus mycelia, including pH, temperature, carbon sources, and nitrogen sources. Based on the assessment of colony diameter and mycelial density, the optimal culture medium, temperature, and pH for mycelial growth were found to be PDA, 25 ℃, and pH 5.0, respectively. Furthermore, the study revealed that the optimal carbon and nitrogen sources for mycelial growth were 1% soluble starch and 2% malt extract, respectively. The other suitable inorganic nitrogen sources were deemed to be 0.1% NH₄H₄PO₄ and 0.1% aspartic acid.

• The Korean Journal of Mycology
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• v.43 no.3
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• pp.149-157
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• 2015

We examined taxonomic characteristics and safety of eight Nuruk molds that are widely used for making soybean paste, soy sauce and alcoholic beverages in Korea. HK1 from Hakyeong Fermentation Co., SW101 from Suwon Fermentation Co., CF1001, CF1002, CF1003 from Chungmoo Fermaentation Co. and KACC 93210 are yellow-Nuruk molds, and SW201 from Suwon Fermentation Co. and CF1005 from Chungmoo Fermentation Co. are white-Nuruk molds. Six strains of yellow-Nuruk molds were identified as Aspergillus oryzae. HK1, SW101, CF1001 and CF1003 of yellow-Nuruk molds have middle length of stipes ($711{\sim}1,121{\mu}m$), and CF1003 (for sake) produced less conidia and more hyphae than HK1, SW101 and CF1001 (for soybean paste). CF 1002 used for soy sauce has shorter stipes ($543{\mu}m$) and is clustered into IBLB-group based on omtA gene analysis although the other yellow-Nuruk molds are clustered into ICAo group. KACC 93210 isolated from traditional Korean Meju has very short stipes (average $270{\mu}m$), and showed velvety colonies although the others showed floccose colonies. The strain has different DNA sequences of omtA gene from other strains in NCBI GenBank as well as strains used in Korea, suggesting that it is unique from other strains published. SW201 and CF1005 of white-Nuruk molds were identified as Aspergillus luchuensis or A. luchuensis mut. Kawachii that is known as safe, non-toxigenic fungus. The six strains of yellow-Nuruk molds did not produce mycotoxins including aflatoxin, cyclopiazonic acid, and sterigmatocystin. Therefore, eight strains of Nuruk molds used for making soy sauce, soybean paste and alcoholic beverages in Korea were proved to be safe in this study.

• Journal of Bio-Environment Control
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• v.19 no.2
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• pp.77-81
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• 2010

The effects of $CO_2$ concentration on fruit-body formation and yield of Pleurotus ferulae (KME65003) mushroom were examined in the growing facilities for bottle cultivation. The $CO_2$ concentration levels in the growing facilities were 500 ppm, 1000 ppm, 1500 ppm and 2000 ppm of $CO_2$, controlled by different ventilation amount. Yield of fruit body was highest of 102.4 g/bottle at 1000 ppm treatment and lowest of 75.1 g/bottle at 2000 ppm. As the $CO_2$ concentration increased up to 2000 ppm, the first pinhead formation and fruit-body growing period took longer, so total growing period took 16 days at 500 ppm, but 23 days at 2000 ppm. The number of pinhead formation was highest of 12.2/bottle at 1500 ppm and valid stipes was highest of 2.8/bottle at 1000 ppm. Fruit body characteristics such as pileus and stipe diameter, and stipe length were not significant at different $CO_2$ concentration. The fruit body ratio of 20~50 g range among the whole fruit body which was classified by weight was highest of 60.3% at 1000 ppm. As a result, the suitable $CO_2$ concentration of growth and yield of Pleurotus ferulae was showed as 1000 ppm.